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The map label for this gene is bioD-B [H]

Identifier: 150008532

GI number: 150008532

Start: 2277578

End: 2278258

Strand: Direct

Name: bioD-B [H]

Synonym: BDI_1918

Alternate gene names: 150008532

Gene position: 2277578-2278258 (Clockwise)

Preceding gene: 150008531

Following gene: 150008534

Centisome position: 47.34

GC content: 49.63

Gene sequence:

>681_bases
ATGAACCATCAAACGAAAGAAAATGTATTATTTATCTCCGGCATAGACACGAACGTCGGAAAGACCGTAGCCACCGGAAT
GATCGCCAAGGCATTGGCGAAAGCCGGAAAGAAAGTGATCACCCAAAAGATGATACAGACCGGATGCGAGCACGTATCGG
AGGATATCGAGGCACACCGCCAAATCCAAGGGCTCCCCTTTACCGACGAGGATACGCAAGGACTGACTTGTCCTTATATC
TTCACCTATCCCTGCTCTCCTCATATGGCGGCGGCGAAAGATGGAAGACGTATCGATTTAGACACGATCACCCAAGCCAC
TCGGCGATTGCGAGAGACCTACGAGTATGTATTACTGGAAGGAGCTGGAGGACTGATGGTTCCGAACGACATGGAATCCC
TCACGATCGATTATGTAAAGGAACAGGCGTATCCCTTGATACTTGTCACGTCCGGCAAGCTGGGAAGTATCAATCATACT
TTGCTCAGCCTGTACGCCTGCGAACGATACGGCATCGAGGTAAAAGCCATCGTCTATAACCAATATCCAAGCATCGACCC
ACTGATCGAGGCGAATACCTTGGAGTATCTAAAGGCCAAATTCCCGGAGATCCCCTTAATCCTCCTTCCGGAAATGAAAA
AGGGAAGCGAGGAGCTTCCCGATATTCGCCCGTTGCTATAA

Upstream 100 bases:

>100_bases
GATCAATAGCGCAGAAGAAAAGGAATAAATCTCTTAGAGACAAGGAGTAATATCCATAGAGACAAAAGATTAATCAAGCA
ACTATTTAAACAACAGAACA

Downstream 100 bases:

>100_bases
CGCCCTATCAAGCCGGATCAGGAAACAGATCCAGTATTTTCTGATCCGCTTTTCTCCGGATCTCCGGTGTAATGCTATCC
TTCACCATTTTCAATACGAA

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2 [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNHQTKENVLFISGIDTNVGKTVATGMIAKALAKAGKKVITQKMIQTGCEHVSEDIEAHRQIQGLPFTDEDTQGLTCPYI
FTYPCSPHMAAAKDGRRIDLDTITQATRRLRETYEYVLLEGAGGLMVPNDMESLTIDYVKEQAYPLILVTSGKLGSINHT
LLSLYACERYGIEVKAIVYNQYPSIDPLIEANTLEYLKAKFPEIPLILLPEMKKGSEELPDIRPLL

Sequences:

>Translated_226_residues
MNHQTKENVLFISGIDTNVGKTVATGMIAKALAKAGKKVITQKMIQTGCEHVSEDIEAHRQIQGLPFTDEDTQGLTCPYI
FTYPCSPHMAAAKDGRRIDLDTITQATRRLRETYEYVLLEGAGGLMVPNDMESLTIDYVKEQAYPLILVTSGKLGSINHT
LLSLYACERYGIEVKAIVYNQYPSIDPLIEANTLEYLKAKFPEIPLILLPEMKKGSEELPDIRPLL
>Mature_226_residues
MNHQTKENVLFISGIDTNVGKTVATGMIAKALAKAGKKVITQKMIQTGCEHVSEDIEAHRQIQGLPFTDEDTQGLTCPYI
FTYPCSPHMAAAKDGRRIDLDTITQATRRLRETYEYVLLEGAGGLMVPNDMESLTIDYVKEQAYPLILVTSGKLGSINHT
LLSLYACERYGIEVKAIVYNQYPSIDPLIEANTLEYLKAKFPEIPLILLPEMKKGSEELPDIRPLL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=174, Percent_Identity=32.7586206896552, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081944, Length=203, Percent_Identity=29.5566502463054, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25174; Mature: 25174

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQTKENVLFISGIDTNVGKTVATGMIAKALAKAGKKVITQKMIQTGCEHVSEDIEAHR
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIQGLPFTDEDTQGLTCPYIFTYPCSPHMAAAKDGRRIDLDTITQATRRLRETYEYVLLE
HHCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCEECHHHHHHHHHHHHHHHHEEEEE
GAGGLMVPNDMESLTIDYVKEQAYPLILVTSGKLGSINHTLLSLYACERYGIEVKAIVYN
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEE
QYPSIDPLIEANTLEYLKAKFPEIPLILLPEMKKGSEELPDIRPLL
CCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNHQTKENVLFISGIDTNVGKTVATGMIAKALAKAGKKVITQKMIQTGCEHVSEDIEAHR
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIQGLPFTDEDTQGLTCPYIFTYPCSPHMAAAKDGRRIDLDTITQATRRLRETYEYVLLE
HHCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCEECHHHHHHHHHHHHHHHHEEEEE
GAGGLMVPNDMESLTIDYVKEQAYPLILVTSGKLGSINHTLLSLYACERYGIEVKAIVYN
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEE
QYPSIDPLIEANTLEYLKAKFPEIPLILLPEMKKGSEELPDIRPLL
CCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]