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The map label for this gene is dnaG [H]

Identifier: 150008464

GI number: 150008464

Start: 2201806

End: 2203923

Strand: Direct

Name: dnaG [H]

Synonym: BDI_1848

Alternate gene names: 150008464

Gene position: 2201806-2203923 (Clockwise)

Preceding gene: 150008450

Following gene: 150008465

Centisome position: 45.76

GC content: 47.97

Gene sequence:

>2118_bases
ATGATCGATCAACCTACCATAGACCGGATATTGGACGCGGCGAACATCGTGGATGTTGTCTCTGAATTCGTGACTCTTCG
GAAACGAGGGATCAACTATGTAGGATTATGCCCATTTCATTCGGACAAATCACCGTCTTTTTACGTATCCCCCTCAAAGA
ATATCTGTAAGTGTTTCGCTTGCGGAGAAGGTGGAACCGCCGTTCACTTCATCATGAAGCATGAGCAACTCAGCTATTTC
GACGCTTTACGCTTTCTGGCGAAAAAATACAATATAGAGATACAGGAACGGGAACTTACCGAGAAAGAGAAACAAATCCG
TAGCGACCGGGAAAGTATGCTTATCGTCAATAGTTGGGCGGGACAATACTTCTCTACTTTATTACATGAGCATGTTGAAG
GGAAAAGCGTAGGTATGCGTTACTTTGCCGAGCGAGGATTGAGGGAAGATATCATCCGTAAATTCCAGCTCGGGTATAGC
TTGGACCAGCGGGATGCTTTATACAAAACCGCTATAAAAGCAGGTTATAAAAAGGAATTCCTCGAGAAAACCGGTTTAGT
TATCGCTTATGATAATGGAAACGTGAACGATCGTTTTCGGGGACGTGTCATCTTTCCCGTCCATACGCTAAGCGGAAAGG
TCGTGGCATTCGGCGGACGTGTATTGAAAAAGGACGAGAAGACCGCGAAGTACGTAAACTCTCCCGAAAGCGAGATTTAT
CATAAGAGTAACGAGCTTTACGGCATCTATTTCGCCAAGCAAGCAATCATGAAACAGGATCGCTGTTTCTTGGTGGAAGG
ATATATGGACGTGATCGGTATGCACCAAGTGGGCGTAGAAAACGTGGTTGCCTCCTCGGGTACGGCGCTCACGCAAGGGC
AAATCCGGCTCATACATCGTTTCACGAATAATATCACGGTATTATACGATGGAGACGCCGCCGGAATCAAAGCGGCCCTC
CGGGGTATCGATATGTTATTGGAAGAAGGGATGAACGTCAAGGTCGTGCTTCTTCCGGCGGGAGAAGATCCGGACTCGTT
TGCCCGTAGCCATAGCGCGAGCGAGTTCGCAGAGTTTATCAGCCAGAACGAGACGGATTTCATCCGTTTCAAGACAAAGT
TACTATTGGCGGAAGCGGGGGGCGATCCGATCAAACGTTCCGCCCTGATCTCGGATATTATCCGTACGGTAGCGATCATA
CCTGATAATATCGCTCGCTCCGTGTATATCCGGGAATGTAGTACGACCATGGAGATAGACGAGCAAGTCTTACTCAACGA
GGTCAATAAGATCCGTCTCAACAAGGAAGAGAACCAAGCGGCTAAATCCGTAAGGAATACACCGCCCGTTCAATCTCCCG
TCAATACGATTCCGGAGTATCCGGATTTCCCCGGCTACCAACCGTATACACCGGAAGAGGCGAATACCCTTCCTCCGGAA
AATATTCCGCCTCCACCGCCCGAGGACTATATTCCGGAAGAGGAAGCGGGTCCGCCCCCTACTCCTCCATACGAGGTTCC
GACGGCTCCCAACATACAGGTCCAACCGAAACGCTCTCCTTTCGAGGCTTACGAATTGGCTCTTTTACGCTATATCGTAC
GATACGGCGAACGTGTCCTCTATGACTACGTAGATGAGGAGACCAACGAGCATGTTATCATGCGGGTCGCCGATTATATC
CGTTTCGATCTGGAACGTGATGACCTTACGTTTTATACGCCGACCTTCAAGCAAATGTTGGACGAGGCTGCCGAGCATTG
CCAGAACGAGGGTTTTATGGCCACTCGTTATTTCCTTGCCCATCCGGACCCGAACATCAGCCGGCTTGCCGCCAACCTTA
TCAGCGATAAGTACCAGCTTAGTAAATATCATACGAAGTTCAGGGAACTGGAGCAAGAAGAAGATAAATTGGATTACTTG
GTACCCCGGGAAATCTATTCTATGAAAGATGCCTATATCCTCTATAAGATAAAGGATATCCAAGCGAAAATCAAAGAGGC
TCAAAACAAGGGAGACATGGAGCTGATCTTTGATTTAATGAAACAGAATGCCCACCTCAACGAGATCAAGAACGTGCTCT
GCAAAGAGCTGGGTGAGCGTATCGTATTAAAAATGTAA

Upstream 100 bases:

>100_bases
ATATAGCAAAAAATAACCAAACACAAGCAAACATCAAGCCTTGTTTGTGTTTGGTTATTTTTCACTACATTTGTGTTCGC
TAAAAAAGGAGTAATTTAAA

Downstream 100 bases:

>100_bases
TAAAAAAACATCTATGAATTTCCTTGAGACAGAAAACGTGGTAAAACAGTACGCCGGGCATCTGGCGCTGAACAAGGTAA
GTATCCAAGTTCCTCAAGGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MIDQPTIDRILDAANIVDVVSEFVTLRKRGINYVGLCPFHSDKSPSFYVSPSKNICKCFACGEGGTAVHFIMKHEQLSYF
DALRFLAKKYNIEIQERELTEKEKQIRSDRESMLIVNSWAGQYFSTLLHEHVEGKSVGMRYFAERGLREDIIRKFQLGYS
LDQRDALYKTAIKAGYKKEFLEKTGLVIAYDNGNVNDRFRGRVIFPVHTLSGKVVAFGGRVLKKDEKTAKYVNSPESEIY
HKSNELYGIYFAKQAIMKQDRCFLVEGYMDVIGMHQVGVENVVASSGTALTQGQIRLIHRFTNNITVLYDGDAAGIKAAL
RGIDMLLEEGMNVKVVLLPAGEDPDSFARSHSASEFAEFISQNETDFIRFKTKLLLAEAGGDPIKRSALISDIIRTVAII
PDNIARSVYIRECSTTMEIDEQVLLNEVNKIRLNKEENQAAKSVRNTPPVQSPVNTIPEYPDFPGYQPYTPEEANTLPPE
NIPPPPPEDYIPEEEAGPPPTPPYEVPTAPNIQVQPKRSPFEAYELALLRYIVRYGERVLYDYVDEETNEHVIMRVADYI
RFDLERDDLTFYTPTFKQMLDEAAEHCQNEGFMATRYFLAHPDPNISRLAANLISDKYQLSKYHTKFRELEQEEDKLDYL
VPREIYSMKDAYILYKIKDIQAKIKEAQNKGDMELIFDLMKQNAHLNEIKNVLCKELGERIVLKM

Sequences:

>Translated_705_residues
MIDQPTIDRILDAANIVDVVSEFVTLRKRGINYVGLCPFHSDKSPSFYVSPSKNICKCFACGEGGTAVHFIMKHEQLSYF
DALRFLAKKYNIEIQERELTEKEKQIRSDRESMLIVNSWAGQYFSTLLHEHVEGKSVGMRYFAERGLREDIIRKFQLGYS
LDQRDALYKTAIKAGYKKEFLEKTGLVIAYDNGNVNDRFRGRVIFPVHTLSGKVVAFGGRVLKKDEKTAKYVNSPESEIY
HKSNELYGIYFAKQAIMKQDRCFLVEGYMDVIGMHQVGVENVVASSGTALTQGQIRLIHRFTNNITVLYDGDAAGIKAAL
RGIDMLLEEGMNVKVVLLPAGEDPDSFARSHSASEFAEFISQNETDFIRFKTKLLLAEAGGDPIKRSALISDIIRTVAII
PDNIARSVYIRECSTTMEIDEQVLLNEVNKIRLNKEENQAAKSVRNTPPVQSPVNTIPEYPDFPGYQPYTPEEANTLPPE
NIPPPPPEDYIPEEEAGPPPTPPYEVPTAPNIQVQPKRSPFEAYELALLRYIVRYGERVLYDYVDEETNEHVIMRVADYI
RFDLERDDLTFYTPTFKQMLDEAAEHCQNEGFMATRYFLAHPDPNISRLAANLISDKYQLSKYHTKFRELEQEEDKLDYL
VPREIYSMKDAYILYKIKDIQAKIKEAQNKGDMELIFDLMKQNAHLNEIKNVLCKELGERIVLKM
>Mature_705_residues
MIDQPTIDRILDAANIVDVVSEFVTLRKRGINYVGLCPFHSDKSPSFYVSPSKNICKCFACGEGGTAVHFIMKHEQLSYF
DALRFLAKKYNIEIQERELTEKEKQIRSDRESMLIVNSWAGQYFSTLLHEHVEGKSVGMRYFAERGLREDIIRKFQLGYS
LDQRDALYKTAIKAGYKKEFLEKTGLVIAYDNGNVNDRFRGRVIFPVHTLSGKVVAFGGRVLKKDEKTAKYVNSPESEIY
HKSNELYGIYFAKQAIMKQDRCFLVEGYMDVIGMHQVGVENVVASSGTALTQGQIRLIHRFTNNITVLYDGDAAGIKAAL
RGIDMLLEEGMNVKVVLLPAGEDPDSFARSHSASEFAEFISQNETDFIRFKTKLLLAEAGGDPIKRSALISDIIRTVAII
PDNIARSVYIRECSTTMEIDEQVLLNEVNKIRLNKEENQAAKSVRNTPPVQSPVNTIPEYPDFPGYQPYTPEEANTLPPE
NIPPPPPEDYIPEEEAGPPPTPPYEVPTAPNIQVQPKRSPFEAYELALLRYIVRYGERVLYDYVDEETNEHVIMRVADYI
RFDLERDDLTFYTPTFKQMLDEAAEHCQNEGFMATRYFLAHPDPNISRLAANLISDKYQLSKYHTKFRELEQEEDKLDYL
VPREIYSMKDAYILYKIKDIQAKIKEAQNKGDMELIFDLMKQNAHLNEIKNVLCKELGERIVLKM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=359, Percent_Identity=35.0974930362117, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 80646; Mature: 80646

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQPTIDRILDAANIVDVVSEFVTLRKRGINYVGLCPFHSDKSPSFYVSPSKNICKCFA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCEEEEEE
CGEGGTAVHFIMKHEQLSYFDALRFLAKKYNIEIQERELTEKEKQIRSDRESMLIVNSWA
ECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCEEEEEHHH
GQYFSTLLHEHVEGKSVGMRYFAERGLREDIIRKFQLGYSLDQRDALYKTAIKAGYKKEF
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LEKTGLVIAYDNGNVNDRFRGRVIFPVHTLSGKVVAFGGRVLKKDEKTAKYVNSPESEIY
HHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCHHCCCHHHHHHCCCCHHHHH
HKSNELYGIYFAKQAIMKQDRCFLVEGYMDVIGMHQVGVENVVASSGTALTQGQIRLIHR
HCCCCEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHCHHHHHHCCCCEECCCHHHEEEE
FTNNITVLYDGDAAGIKAALRGIDMLLEEGMNVKVVLLPAGEDPDSFARSHSASEFAEFI
HHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHH
SQNETDFIRFKTKLLLAEAGGDPIKRSALISDIIRTVAIIPDNIARSVYIRECSTTMEID
HCCCCHHHEEHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEHHHCCCHHHHH
EQVLLNEVNKIRLNKEENQAAKSVRNTPPVQSPVNTIPEYPDFPGYQPYTPEEANTLPPE
HHHHHHHHHHEECCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
NIPPPPPEDYIPEEEAGPPPTPPYEVPTAPNIQVQPKRSPFEAYELALLRYIVRYGERVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
YDYVDEETNEHVIMRVADYIRFDLERDDLTFYTPTFKQMLDEAAEHCQNEGFMATRYFLA
HHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEE
HPDPNISRLAANLISDKYQLSKYHTKFRELEQEEDKLDYLVPREIYSMKDAYILYKIKDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEHHH
QAKIKEAQNKGDMELIFDLMKQNAHLNEIKNVLCKELGERIVLKM
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCCC
>Mature Secondary Structure
MIDQPTIDRILDAANIVDVVSEFVTLRKRGINYVGLCPFHSDKSPSFYVSPSKNICKCFA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCEEEEEE
CGEGGTAVHFIMKHEQLSYFDALRFLAKKYNIEIQERELTEKEKQIRSDRESMLIVNSWA
ECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCEEEEEHHH
GQYFSTLLHEHVEGKSVGMRYFAERGLREDIIRKFQLGYSLDQRDALYKTAIKAGYKKEF
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LEKTGLVIAYDNGNVNDRFRGRVIFPVHTLSGKVVAFGGRVLKKDEKTAKYVNSPESEIY
HHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCHHCCCHHHHHHCCCCHHHHH
HKSNELYGIYFAKQAIMKQDRCFLVEGYMDVIGMHQVGVENVVASSGTALTQGQIRLIHR
HCCCCEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHCHHHHHHCCCCEECCCHHHEEEE
FTNNITVLYDGDAAGIKAALRGIDMLLEEGMNVKVVLLPAGEDPDSFARSHSASEFAEFI
HHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHH
SQNETDFIRFKTKLLLAEAGGDPIKRSALISDIIRTVAIIPDNIARSVYIRECSTTMEID
HCCCCHHHEEHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEHHHCCCHHHHH
EQVLLNEVNKIRLNKEENQAAKSVRNTPPVQSPVNTIPEYPDFPGYQPYTPEEANTLPPE
HHHHHHHHHHEECCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
NIPPPPPEDYIPEEEAGPPPTPPYEVPTAPNIQVQPKRSPFEAYELALLRYIVRYGERVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHH
YDYVDEETNEHVIMRVADYIRFDLERDDLTFYTPTFKQMLDEAAEHCQNEGFMATRYFLA
HHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEE
HPDPNISRLAANLISDKYQLSKYHTKFRELEQEEDKLDYLVPREIYSMKDAYILYKIKDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEHHH
QAKIKEAQNKGDMELIFDLMKQNAHLNEIKNVLCKELGERIVLKM
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]