Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 150008444

GI number: 150008444

Start: 2177325

End: 2178023

Strand: Direct

Name: rsmE [H]

Synonym: BDI_1826

Alternate gene names: 150008444

Gene position: 2177325-2178023 (Clockwise)

Preceding gene: 150008443

Following gene: 150008445

Centisome position: 45.25

GC content: 47.35

Gene sequence:

>699_bases
ATGCAGATTTTTTATACACCAGATATAGCTATCAAGCCGGAATTACCGGAAGAAGAAGCAGGACATTGTATACGGGTACT
TCGTCTAGGTGAAGGCGATGAGATAGTATTGACGGATGGGCAAGGCTCTTTTTATAAAGCGGCTATTAGCCGGGCGCATC
CCAAACATTGTGAGGTGACCCTCTTAGAGAGTTGGAAACAACCCGATCTCTGGAACTTCAATCTCCATATAGCCATAGCT
CCTACCAAAAACATGGACCGGATGGAGTGGTTCGTGGAGAAAGCGACGGAAATAGGGATCAATGCGATCACCTGTTTGAA
TTGTCGTTTCTCGGAGCGTAAAGAGATCAAGCCGATTCGATTGGAGAAGATATTGGTCAGTGCCATGAAGCAATCCCAGA
AAGCGACTTTGCCGGCGTTAGAAGGAATGACAGATTTCAAGAAGTTTGTCGCCACTCCCTTTGATGGGCGTAAGTTTATC
GCCCATTGCGAGGAAGGCGAGAAGCCACTATTAAAACATACGTATCAGCCGGGAGAGAATGCCTTAATCCTCATCGGACC
GGAAGGTGATTTTAGCCCGGAAGAAATCAAACTGGCTCAAGAGAATGGCTTCGAGCCGATCTCTTTAGGAGAGAGCCGAC
TGCGTACGGAAACGGCCGCGCTAGTGGCTTGCCATACGATTCATGTGTTAAATCAATAG

Upstream 100 bases:

>100_bases
TGATCGGCGGAGCCTTGGTCTCTGTGCTATCGGCTACTATAATTGGTATGATCCTTGGGTAATTGAAAATTGAGAATTGA
GAATTGAAAATTAGATGGTC

Downstream 100 bases:

>100_bases
AATAATCCTATTTCCATTAAAAATACGTTAATCCTCATAAGGGTTATTAAGCAAATTGCTACATTTGTAATGATATTAAC
AAATCTAATCTAATAACAAT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MQIFYTPDIAIKPELPEEEAGHCIRVLRLGEGDEIVLTDGQGSFYKAAISRAHPKHCEVTLLESWKQPDLWNFNLHIAIA
PTKNMDRMEWFVEKATEIGINAITCLNCRFSERKEIKPIRLEKILVSAMKQSQKATLPALEGMTDFKKFVATPFDGRKFI
AHCEEGEKPLLKHTYQPGENALILIGPEGDFSPEEIKLAQENGFEPISLGESRLRTETAALVACHTIHVLNQ

Sequences:

>Translated_232_residues
MQIFYTPDIAIKPELPEEEAGHCIRVLRLGEGDEIVLTDGQGSFYKAAISRAHPKHCEVTLLESWKQPDLWNFNLHIAIA
PTKNMDRMEWFVEKATEIGINAITCLNCRFSERKEIKPIRLEKILVSAMKQSQKATLPALEGMTDFKKFVATPFDGRKFI
AHCEEGEKPLLKHTYQPGENALILIGPEGDFSPEEIKLAQENGFEPISLGESRLRTETAALVACHTIHVLNQ
>Mature_232_residues
MQIFYTPDIAIKPELPEEEAGHCIRVLRLGEGDEIVLTDGQGSFYKAAISRAHPKHCEVTLLESWKQPDLWNFNLHIAIA
PTKNMDRMEWFVEKATEIGINAITCLNCRFSERKEIKPIRLEKILVSAMKQSQKATLPALEGMTDFKKFVATPFDGRKFI
AHCEEGEKPLLKHTYQPGENALILIGPEGDFSPEEIKLAQENGFEPISLGESRLRTETAALVACHTIHVLNQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=222, Percent_Identity=32.8828828828829, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26166; Mature: 26166

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIFYTPDIAIKPELPEEEAGHCIRVLRLGEGDEIVLTDGQGSFYKAAISRAHPKHCEVT
CEEEECCCEEECCCCCHHHHCCEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCEEE
LLESWKQPDLWNFNLHIAIAPTKNMDRMEWFVEKATEIGINAITCLNCRFSERKEIKPIR
EECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
LEKILVSAMKQSQKATLPALEGMTDFKKFVATPFDGRKFIAHCEEGEKPLLKHTYQPGEN
HHHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCCHHEEECCCCCCCHHHCCCCCCCC
ALILIGPEGDFSPEEIKLAQENGFEPISLGESRLRTETAALVACHTIHVLNQ
EEEEECCCCCCCHHHEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MQIFYTPDIAIKPELPEEEAGHCIRVLRLGEGDEIVLTDGQGSFYKAAISRAHPKHCEVT
CEEEECCCEEECCCCCHHHHCCEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCEEE
LLESWKQPDLWNFNLHIAIAPTKNMDRMEWFVEKATEIGINAITCLNCRFSERKEIKPIR
EECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
LEKILVSAMKQSQKATLPALEGMTDFKKFVATPFDGRKFIAHCEEGEKPLLKHTYQPGEN
HHHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCCHHEEECCCCCCCHHHCCCCCCCC
ALILIGPEGDFSPEEIKLAQENGFEPISLGESRLRTETAALVACHTIHVLNQ
EEEEECCCCCCCHHHEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]