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The map label for this gene is glpK

Identifier: 150008434

GI number: 150008434

Start: 2164569

End: 2166068

Strand: Reverse

Name: glpK

Synonym: BDI_1816

Alternate gene names: 150008434

Gene position: 2166068-2164569 (Counterclockwise)

Preceding gene: 150008435

Following gene: 150008433

Centisome position: 45.02

GC content: 50.07

Gene sequence:

>1500_bases
ATGAATTTTCGAAATAAATATGTGTTGGCGATCGATCAGGGAACCACTAGCTCCCGGGCTATCCTGTTTAATCATCAAGG
AACTATTATTACGTTGGCTCAGAAGCCATTCCAACAGTATTTCCCGAAACCGGGCTGGGTAGAGCACGACCCGAATGAGA
TTTGGTACACCCAATCGTCTGTCATCAAGGAGGCGATGGCAAAAGCGGATGTCACGGATAGCCATATCGCCTGTATCGGA
ATCGCTAACCAACGGGAGACTACCATCATCTGGGACCGGGAGACTGGCTTTCCCGTATACAATGCGATCGTATGGCAGGA
TCGCCGTACGGCGGATTATTGCGAGGAACTTAAATCCCAAGGCTGGGCGGAACGTATCCTGCAGAAGACCGGGTTGGTGA
TCGACGCTTATTTCTCCGCCACGAAGATAAAATGGATATTGGATAACGTAAAGGGTATCCGGGAGCGTGCGGAGCGGGGA
GAACTATGCTTTGGAACGGTAGATACTTGGCTGGTTTGGAAACTTACCCGGGGCGCTCAATTCATTACGGATGTGACGAA
CGCTTCCCGTACCATGCTTTTCAATATCCGTACTTTGCAATGGGACCAAGAGTTATTGGACTTGTTTACAATACCTGCCT
CGATGTTACCGCAAGTGAAAGCCTGTAGCGAGGTTTACTGCGAGACATCAACTCCTATTTTTAAGAAAGGCATTCCGGTA
TCCGGTATGGCCGGCGACCAACAGGCGGCTCTGTTCGGCCAGCTCTGCGTAGAGGATGGTATGATCAAGACAACCTACGG
AACCGGCTGCTTTATGATCCTGAATACCGGTAAGGAACCCGTTCTTTCCCAGAATAACCTATTGACAACGATCGCATGGA
AATTAGGGGATCAGACGACCTATGCGCTGGAAGGTAGTGTCTTTGTGGGAGGTGCCGTCGTTCAATGGTTGAGGGATGGG
ATCGGCTTGATTCCGAATGCCTCGATTACGGAGCAAATGGCGAAATCCGTCAGTGATAATGGGGGCGTTTATTTTGTGCC
GGCGTTAACAGGCTTGGGTGCGCCGTATTGGGATCAATACGCACGTGGCGCTATTATCGGGATTACCCGCGGAACTACGG
CGGCCCACTTGACCCGTGCGGCTTTGGAGGGCATCTGCTATCAAGTATATGATGTATTGATGGCCATGGAAAACGATATT
CACGCCAAGCCGAAAGAGATTCGGGTAGATGGCGGTGCTATCGCTAATAACTTCTTGATGCAATTCCAGTCCGATATCTG
CCGCTGTCCGGTCGTACGTCCGAATGTATTGGAGACGACTGCTTTGGGAGCCGCTTATTTGGCGGGCTTGGCGATCGGCT
ATTGGAAGGATATCGATGAACTGAAAGAACAATGGTGCTTGGATAAGGTCTTTAACCCACAAATGAAAGAGGATACCGCC
CGTAAATTATTGAACGAATGGCATAAGGCGGTGGGGCGTTCTCAGAATTGGGCTGAATAA

Upstream 100 bases:

>100_bases
ACGAGACTTGGACCTCTGCTCAGGTAAAGGCGTTTAATGAGGTTGCCTCGAATTATATACTGAATTGACAAATTGATAAA
CGATACTAATATAGAAGGAT

Downstream 100 bases:

>100_bases
TGAAAATCATCTTATAGGCAGATTAACAAGTTAACAAATTCTTAAATTATTATCTTATGTCTCCATTTTTAGCGGAATTA
TTAGGGACTATGCTCTTGAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNFRNKYVLAIDQGTTSSRAILFNHQGTIITLAQKPFQQYFPKPGWVEHDPNEIWYTQSSVIKEAMAKADVTDSHIACIG
IANQRETTIIWDRETGFPVYNAIVWQDRRTADYCEELKSQGWAERILQKTGLVIDAYFSATKIKWILDNVKGIRERAERG
ELCFGTVDTWLVWKLTRGAQFITDVTNASRTMLFNIRTLQWDQELLDLFTIPASMLPQVKACSEVYCETSTPIFKKGIPV
SGMAGDQQAALFGQLCVEDGMIKTTYGTGCFMILNTGKEPVLSQNNLLTTIAWKLGDQTTYALEGSVFVGGAVVQWLRDG
IGLIPNASITEQMAKSVSDNGGVYFVPALTGLGAPYWDQYARGAIIGITRGTTAAHLTRAALEGICYQVYDVLMAMENDI
HAKPKEIRVDGGAIANNFLMQFQSDICRCPVVRPNVLETTALGAAYLAGLAIGYWKDIDELKEQWCLDKVFNPQMKEDTA
RKLLNEWHKAVGRSQNWAE

Sequences:

>Translated_499_residues
MNFRNKYVLAIDQGTTSSRAILFNHQGTIITLAQKPFQQYFPKPGWVEHDPNEIWYTQSSVIKEAMAKADVTDSHIACIG
IANQRETTIIWDRETGFPVYNAIVWQDRRTADYCEELKSQGWAERILQKTGLVIDAYFSATKIKWILDNVKGIRERAERG
ELCFGTVDTWLVWKLTRGAQFITDVTNASRTMLFNIRTLQWDQELLDLFTIPASMLPQVKACSEVYCETSTPIFKKGIPV
SGMAGDQQAALFGQLCVEDGMIKTTYGTGCFMILNTGKEPVLSQNNLLTTIAWKLGDQTTYALEGSVFVGGAVVQWLRDG
IGLIPNASITEQMAKSVSDNGGVYFVPALTGLGAPYWDQYARGAIIGITRGTTAAHLTRAALEGICYQVYDVLMAMENDI
HAKPKEIRVDGGAIANNFLMQFQSDICRCPVVRPNVLETTALGAAYLAGLAIGYWKDIDELKEQWCLDKVFNPQMKEDTA
RKLLNEWHKAVGRSQNWAE
>Mature_499_residues
MNFRNKYVLAIDQGTTSSRAILFNHQGTIITLAQKPFQQYFPKPGWVEHDPNEIWYTQSSVIKEAMAKADVTDSHIACIG
IANQRETTIIWDRETGFPVYNAIVWQDRRTADYCEELKSQGWAERILQKTGLVIDAYFSATKIKWILDNVKGIRERAERG
ELCFGTVDTWLVWKLTRGAQFITDVTNASRTMLFNIRTLQWDQELLDLFTIPASMLPQVKACSEVYCETSTPIFKKGIPV
SGMAGDQQAALFGQLCVEDGMIKTTYGTGCFMILNTGKEPVLSQNNLLTTIAWKLGDQTTYALEGSVFVGGAVVQWLRDG
IGLIPNASITEQMAKSVSDNGGVYFVPALTGLGAPYWDQYARGAIIGITRGTTAAHLTRAALEGICYQVYDVLMAMENDI
HAKPKEIRVDGGAIANNFLMQFQSDICRCPVVRPNVLETTALGAAYLAGLAIGYWKDIDELKEQWCLDKVFNPQMKEDTA
RKLLNEWHKAVGRSQNWAE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=43.7375745526839, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=43.5387673956262, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=43.5387673956262, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI42794763, Length=508, Percent_Identity=43.1102362204724, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=34.3195266272189, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=59.3561368209256, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1789987, Length=503, Percent_Identity=23.8568588469185, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790005, Length=466, Percent_Identity=23.175965665236, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787789, Length=504, Percent_Identity=23.4126984126984, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789168, Length=491, Percent_Identity=23.4215885947047, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789138, Length=472, Percent_Identity=20.9745762711864, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=502, Percent_Identity=41.6334661354582, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=25.8252427184466, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=261, Percent_Identity=32.9501915708812, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=547, Percent_Identity=36.9287020109689, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=42.0948616600791, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655555, Length=466, Percent_Identity=42.274678111588, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655560, Length=466, Percent_Identity=42.274678111588, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655570, Length=466, Percent_Identity=42.274678111588, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655574, Length=466, Percent_Identity=42.274678111588, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655565, Length=466, Percent_Identity=42.274678111588, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24654573, Length=433, Percent_Identity=42.9561200923788, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=32.1292775665399, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=33.2657200811359, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=30.6796116504854, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=30.0567107750473, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24656372, Length=373, Percent_Identity=32.171581769437, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI45552909, Length=309, Percent_Identity=30.7443365695793, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_PARD8 (A6LCZ1)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303177.1
- ProteinModelPortal:   A6LCZ1
- SMR:   A6LCZ1
- STRING:   A6LCZ1
- GeneID:   5306966
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1816
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- BioCyc:   PDIS435591:BDI_1816-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55778; Mature: 55778

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 15-15 BINDING 19-19 BINDING 85-85 BINDING 137-137 BINDING 246-246 BINDING 268-268 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRNKYVLAIDQGTTSSRAILFNHQGTIITLAQKPFQQYFPKPGWVEHDPNEIWYTQSS
CCCCCCEEEEEECCCCCCEEEEEECCCCEEEECCCHHHHHCCCCCCCCCCCCCEEECHHH
VIKEAMAKADVTDSHIACIGIANQRETTIIWDRETGFPVYNAIVWQDRRTADYCEELKSQ
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHC
GWAERILQKTGLVIDAYFSATKIKWILDNVKGIRERAERGELCFGTVDTWLVWKLTRGAQ
CHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCH
FITDVTNASRTMLFNIRTLQWDQELLDLFTIPASMLPQVKACSEVYCETSTPIFKKGIPV
HHHHHCCCCEEEEEEEEEEHHHHHHHHHHHCCHHHCHHHHHHHHHHCCCCCCHHHCCCCC
SGMAGDQQAALFGQLCVEDGMIKTTYGTGCFMILNTGKEPVLSQNNLLTTIAWKLGDQTT
CCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEEECCCCEE
YALEGSVFVGGAVVQWLRDGIGLIPNASITEQMAKSVSDNGGVYFVPALTGLGAPYWDQY
EEECCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHH
ARGAIIGITRGTTAAHLTRAALEGICYQVYDVLMAMENDIHAKPKEIRVDGGAIANNFLM
HCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHH
QFQSDICRCPVVRPNVLETTALGAAYLAGLAIGYWKDIDELKEQWCLDKVFNPQMKEDTA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RKLLNEWHKAVGRSQNWAE
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNFRNKYVLAIDQGTTSSRAILFNHQGTIITLAQKPFQQYFPKPGWVEHDPNEIWYTQSS
CCCCCCEEEEEECCCCCCEEEEEECCCCEEEECCCHHHHHCCCCCCCCCCCCCEEECHHH
VIKEAMAKADVTDSHIACIGIANQRETTIIWDRETGFPVYNAIVWQDRRTADYCEELKSQ
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHC
GWAERILQKTGLVIDAYFSATKIKWILDNVKGIRERAERGELCFGTVDTWLVWKLTRGAQ
CHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCH
FITDVTNASRTMLFNIRTLQWDQELLDLFTIPASMLPQVKACSEVYCETSTPIFKKGIPV
HHHHHCCCCEEEEEEEEEEHHHHHHHHHHHCCHHHCHHHHHHHHHHCCCCCCHHHCCCCC
SGMAGDQQAALFGQLCVEDGMIKTTYGTGCFMILNTGKEPVLSQNNLLTTIAWKLGDQTT
CCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEEECCCCEE
YALEGSVFVGGAVVQWLRDGIGLIPNASITEQMAKSVSDNGGVYFVPALTGLGAPYWDQY
EEECCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHH
ARGAIIGITRGTTAAHLTRAALEGICYQVYDVLMAMENDIHAKPKEIRVDGGAIANNFLM
HCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHH
QFQSDICRCPVVRPNVLETTALGAAYLAGLAIGYWKDIDELKEQWCLDKVFNPQMKEDTA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RKLLNEWHKAVGRSQNWAE
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA