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The map label for this gene is glpF [H]

Identifier: 150008433

GI number: 150008433

Start: 2163775

End: 2164512

Strand: Reverse

Name: glpF [H]

Synonym: BDI_1815

Alternate gene names: 150008433

Gene position: 2164512-2163775 (Counterclockwise)

Preceding gene: 150008434

Following gene: 150008425

Centisome position: 44.99

GC content: 47.7

Gene sequence:

>738_bases
ATGTCTCCATTTTTAGCGGAATTATTAGGGACTATGCTCTTGATCCTGATGGGAGGAGGAGTTGTAGCGAATGTCTGTTT
GTCGAAAACAAAAGGCAATGGAGCGGGCTGGATAGCGATAACGACCGCTTGGGCTTTAGGCGTATTTATCGGTGTTGTGG
TAGCCGGCCCTTATAGTGGCGCACATTTGAATCCGGCGGTATCGATCGGCTTGGCGATCGGAGGGACTTTCCCTTGGTGT
GATGTATGTACTTATATAGCCGGGCAAATGATAGGTGCCGCTTTGGGCGCTTTATTGGTCTGGCTGGTTTATAAGGATCA
CTTCAACGCTACGGATGATCCGGATGCGGAATTAGGCGTATTCTGTACGAGTCCTGCTATCAAAGATTATCCAATTAATT
TCTTAGGTGAGATGATCGGTACGTTCGCCTTGATGTTTGTCGTATTTTTTATAACGGACGGAGAACTAACCTACGAAAGC
AATAGTACCTTGCCGATAGGTTTAGGTTCGGTAGGTGCCATTCCGGTAGCGTTTACGGTCTGGGTAATCGGCCTTTCCTT
AGGTGGAACTACCGGTTACGCGATTAACCCTGCCCGTGATTTAGCTCCCCGGTTCATACACTTTATTTTGCCGATCAAGG
GAAAAGGCAGCAGCCATTGGGAGTATGCTTGGGTACCTGTTTTAGGGCCGATAGCTGGGGCCGCTATTGCCGGTATGCTC
TATTATGTATTGAAATAA

Upstream 100 bases:

>100_bases
AATGGCATAAGGCGGTGGGGCGTTCTCAGAATTGGGCTGAATAATGAAAATCATCTTATAGGCAGATTAACAAGTTAACA
AATTCTTAAATTATTATCTT

Downstream 100 bases:

>100_bases
TCTCTTGTATCATCCGGATCACTTTCGGAACCGCCGCTTTTACCTCGGGTGTCAGATCCATTCCGACCTCGTTGATATTG
AGGGCGGAGATGACAATGAG

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSPFLAELLGTMLLILMGGGVVANVCLSKTKGNGAGWIAITTAWALGVFIGVVVAGPYSGAHLNPAVSIGLAIGGTFPWC
DVCTYIAGQMIGAALGALLVWLVYKDHFNATDDPDAELGVFCTSPAIKDYPINFLGEMIGTFALMFVVFFITDGELTYES
NSTLPIGLGSVGAIPVAFTVWVIGLSLGGTTGYAINPARDLAPRFIHFILPIKGKGSSHWEYAWVPVLGPIAGAAIAGML
YYVLK

Sequences:

>Translated_245_residues
MSPFLAELLGTMLLILMGGGVVANVCLSKTKGNGAGWIAITTAWALGVFIGVVVAGPYSGAHLNPAVSIGLAIGGTFPWC
DVCTYIAGQMIGAALGALLVWLVYKDHFNATDDPDAELGVFCTSPAIKDYPINFLGEMIGTFALMFVVFFITDGELTYES
NSTLPIGLGSVGAIPVAFTVWVIGLSLGGTTGYAINPARDLAPRFIHFILPIKGKGSSHWEYAWVPVLGPIAGAAIAGML
YYVLK
>Mature_244_residues
SPFLAELLGTMLLILMGGGVVANVCLSKTKGNGAGWIAITTAWALGVFIGVVVAGPYSGAHLNPAVSIGLAIGGTFPWCD
VCTYIAGQMIGAALGALLVWLVYKDHFNATDDPDAELGVFCTSPAIKDYPINFLGEMIGTFALMFVVFFITDGELTYESN
STLPIGLGSVGAIPVAFTVWVIGLSLGGTTGYAINPARDLAPRFIHFILPIKGKGSSHWEYAWVPVLGPIAGAAIAGMLY
YVLK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=254, Percent_Identity=38.1889763779528, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI4502187, Length=253, Percent_Identity=35.1778656126482, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI157266307, Length=249, Percent_Identity=34.136546184739, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI22538420, Length=253, Percent_Identity=32.8063241106719, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI310133356, Length=193, Percent_Identity=33.160621761658, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI310114181, Length=193, Percent_Identity=33.160621761658, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1790362, Length=257, Percent_Identity=32.295719844358, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994009, Length=238, Percent_Identity=34.8739495798319, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531429, Length=249, Percent_Identity=32.1285140562249, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531431, Length=249, Percent_Identity=32.1285140562249, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=250, Percent_Identity=34.8, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71992966, Length=257, Percent_Identity=30.7392996108949, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=29.9595141700405, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=29.9595141700405, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=252, Percent_Identity=27.3809523809524, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=239, Percent_Identity=25.9414225941423, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=36.5740740740741, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322985, Length=295, Percent_Identity=29.4915254237288, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24652747, Length=244, Percent_Identity=28.6885245901639, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45551084, Length=244, Percent_Identity=28.6885245901639, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25635; Mature: 25504

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFLAELLGTMLLILMGGGVVANVCLSKTKGNGAGWIAITTAWALGVFIGVVVAGPYSG
CCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC
AHLNPAVSIGLAIGGTFPWCDVCTYIAGQMIGAALGALLVWLVYKDHFNATDDPDAELGV
CCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
FCTSPAIKDYPINFLGEMIGTFALMFVVFFITDGELTYESNSTLPIGLGSVGAIPVAFTV
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHHHHH
WVIGLSLGGTTGYAINPARDLAPRFIHFILPIKGKGSSHWEYAWVPVLGPIAGAAIAGML
HHHHHHCCCCCCCEECCHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHH
YYVLK
HHHCC
>Mature Secondary Structure 
SPFLAELLGTMLLILMGGGVVANVCLSKTKGNGAGWIAITTAWALGVFIGVVVAGPYSG
CHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC
AHLNPAVSIGLAIGGTFPWCDVCTYIAGQMIGAALGALLVWLVYKDHFNATDDPDAELGV
CCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
FCTSPAIKDYPINFLGEMIGTFALMFVVFFITDGELTYESNSTLPIGLGSVGAIPVAFTV
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHHHHH
WVIGLSLGGTTGYAINPARDLAPRFIHFILPIKGKGSSHWEYAWVPVLGPIAGAAIAGML
HHHHHHCCCCCCCEECCHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHH
YYVLK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]