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The map label for this gene is hupA [H]

Identifier: 150008429

GI number: 150008429

Start: 2159747

End: 2160844

Strand: Direct

Name: hupA [H]

Synonym: BDI_1811

Alternate gene names: 150008429

Gene position: 2159747-2160844 (Clockwise)

Preceding gene: 150008428

Following gene: 150008430

Centisome position: 44.89

GC content: 53.37

Gene sequence:

>1098_bases
ATGGAAAATAAAAGACCGTCCGTTTACGAAGAGTGCCGTCAGAAAGGCATCTCCCGCCGGGACTTCCTGAAGTTCTGTAC
CACCATGGCCGCCCTTATGGGATTGGAGGCTTCGGGAGTGGCACAGGTAGTAAATGCCTTGGAGACGAAACCCCGCCTTC
CGATCATCTGGCTACACCTACAGGAATGTACCTGTTGTACCGAATCGTTTATCCGTGCCGCCCACCCGATCGTGGCGACC
TTGTTGTTAGACAAGATTTCCCTAGACTATACCGAGACGCTGATGGCCGCCGCCGGCGAACAGGCCGAGGCCGCTAAGGA
AGAGACCATGAAAAAGTATTACGGCAATTATCTACTGATGATCGAGGGGTCGCTACCTACCAAGGACGAGGCTTATTGTT
GCGTAGGCGGCAAGAGTGCCTTGCAGATCACCGAAGAGGCCGCCGCAGGAGCTAAAGCGATCATCGCTTGGGGCAACTGC
GCTTCCGCCGGTTGCGTGCAAGCGGCGAACCCGAATCCTACCGGCGCTAAAGGTATTCATAAAGTCATCAAAGGTAAACC
TATTATTAATGTACAAGGCTGTCCTCCGATCGCCGACGTGATGGCGGGTGTCATTATTTATATGTTGACTTTCGAACGCA
TGCCGCAATTGGACGGATTGGGCCGTCCGAAGATGTTCTACTCCCGCCGAGTACATGATACGTGCTACCGCCGTGCGAAT
TTCGACGCCGGCTTGTTCGTGGAGAGCTTCGACGATGAGAACGCCAAGCATGGCTACTGCTTGTATAAAGTGGGATGCAA
AGGTCCGAGCACCTATAACTCGTGCGGTATCATCAAATGGAACGAGGGCACCAGTTACCCGATCCAGAGCGGTCACCCCT
GTTTGGGTTGCAGCGAGGAGAATTTCTGGGACAACAGCCCGTTCTATAAACGTATGCCCGATATACACGGATTCGGTATC
GAGGCCACGGCGGATCAGATCGGTCTGGCTCTAGGAGCCGCTACCGCCGCCGGTATCGCCGTACATGCCGTAGCAACTAA
CATACGGAAAAAAGAGCTTATCGACAATGATGAGCCGGAAAGTAAATCTACGATCTAA

Upstream 100 bases:

>100_bases
CGGGTCAAGATGAAGACATTATACGGTAACTACCGGATCATCGATATGCTAAGCGGGGAACAACTCCCCCGTATTTGTTA
GAATACTTAATTGCTGAGAT

Downstream 100 bases:

>100_bases
TAAAACACTACTATCATGTCAGAAAGAATTGTCATAGACCCCATTACCCGTATAGAAGGCCACCTGCGGGTAGAGGCTGA
AATAGAAGGTGGCACGATCA

Product: putative uptake hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MENKRPSVYEECRQKGISRRDFLKFCTTMAALMGLEASGVAQVVNALETKPRLPIIWLHLQECTCCTESFIRAAHPIVAT
LLLDKISLDYTETLMAAAGEQAEAAKEETMKKYYGNYLLMIEGSLPTKDEAYCCVGGKSALQITEEAAAGAKAIIAWGNC
ASAGCVQAANPNPTGAKGIHKVIKGKPIINVQGCPPIADVMAGVIIYMLTFERMPQLDGLGRPKMFYSRRVHDTCYRRAN
FDAGLFVESFDDENAKHGYCLYKVGCKGPSTYNSCGIIKWNEGTSYPIQSGHPCLGCSEENFWDNSPFYKRMPDIHGFGI
EATADQIGLALGAATAAGIAVHAVATNIRKKELIDNDEPESKSTI

Sequences:

>Translated_365_residues
MENKRPSVYEECRQKGISRRDFLKFCTTMAALMGLEASGVAQVVNALETKPRLPIIWLHLQECTCCTESFIRAAHPIVAT
LLLDKISLDYTETLMAAAGEQAEAAKEETMKKYYGNYLLMIEGSLPTKDEAYCCVGGKSALQITEEAAAGAKAIIAWGNC
ASAGCVQAANPNPTGAKGIHKVIKGKPIINVQGCPPIADVMAGVIIYMLTFERMPQLDGLGRPKMFYSRRVHDTCYRRAN
FDAGLFVESFDDENAKHGYCLYKVGCKGPSTYNSCGIIKWNEGTSYPIQSGHPCLGCSEENFWDNSPFYKRMPDIHGFGI
EATADQIGLALGAATAAGIAVHAVATNIRKKELIDNDEPESKSTI
>Mature_365_residues
MENKRPSVYEECRQKGISRRDFLKFCTTMAALMGLEASGVAQVVNALETKPRLPIIWLHLQECTCCTESFIRAAHPIVAT
LLLDKISLDYTETLMAAAGEQAEAAKEETMKKYYGNYLLMIEGSLPTKDEAYCCVGGKSALQITEEAAAGAKAIIAWGNC
ASAGCVQAANPNPTGAKGIHKVIKGKPIINVQGCPPIADVMAGVIIYMLTFERMPQLDGLGRPKMFYSRRVHDTCYRRAN
FDAGLFVESFDDENAKHGYCLYKVGCKGPSTYNSCGIIKWNEGTSYPIQSGHPCLGCSEENFWDNSPFYKRMPDIHGFGI
EATADQIGLALGAATAAGIAVHAVATNIRKKELIDNDEPESKSTI

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=332, Percent_Identity=55.421686746988, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1789371, Length=287, Percent_Identity=44.9477351916376, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39758; Mature: 39758

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKRPSVYEECRQKGISRRDFLKFCTTMAALMGLEASGVAQVVNALETKPRLPIIWLHL
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEE
QECTCCTESFIRAAHPIVATLLLDKISLDYTETLMAAAGEQAEAAKEETMKKYYGNYLLM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEE
IEGSLPTKDEAYCCVGGKSALQITEEAAAGAKAIIAWGNCASAGCVQAANPNPTGAKGIH
EECCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCHHHHH
KVIKGKPIINVQGCPPIADVMAGVIIYMLTFERMPQLDGLGRPKMFYSRRVHDTCYRRAN
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
FDAGLFVESFDDENAKHGYCLYKVGCKGPSTYNSCGIIKWNEGTSYPIQSGHPCLGCSEE
CCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
NFWDNSPFYKRMPDIHGFGIEATADQIGLALGAATAAGIAVHAVATNIRKKELIDNDEPE
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SKSTI
CCCCC
>Mature Secondary Structure
MENKRPSVYEECRQKGISRRDFLKFCTTMAALMGLEASGVAQVVNALETKPRLPIIWLHL
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEE
QECTCCTESFIRAAHPIVATLLLDKISLDYTETLMAAAGEQAEAAKEETMKKYYGNYLLM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEE
IEGSLPTKDEAYCCVGGKSALQITEEAAAGAKAIIAWGNCASAGCVQAANPNPTGAKGIH
EECCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCHHHHH
KVIKGKPIINVQGCPPIADVMAGVIIYMLTFERMPQLDGLGRPKMFYSRRVHDTCYRRAN
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
FDAGLFVESFDDENAKHGYCLYKVGCKGPSTYNSCGIIKWNEGTSYPIQSGHPCLGCSEE
CCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
NFWDNSPFYKRMPDIHGFGIEATADQIGLALGAATAAGIAVHAVATNIRKKELIDNDEPE
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SKSTI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2325631 [H]