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The map label for this gene is hupB [H]

Identifier: 150008430

GI number: 150008430

Start: 2160860

End: 2162581

Strand: Direct

Name: hupB [H]

Synonym: BDI_1812

Alternate gene names: 150008430

Gene position: 2160860-2162581 (Clockwise)

Preceding gene: 150008429

Following gene: 150008431

Centisome position: 44.91

GC content: 49.71

Gene sequence:

>1722_bases
ATGTCAGAAAGAATTGTCATAGACCCCATTACCCGTATAGAAGGCCACCTGCGGGTAGAGGCTGAAATAGAAGGTGGCAC
GATCAAGAACGCTTATAGCTCGGGTACTATGGTGCGGGGCATCGAGAATATCGTAAAAGACCGGGATCCCCGGGATGTAT
GGGCTTTCGTAGGCCGTGTTTGCGGCGTATGTACTTCCATCCACTCGCTTGCCTCGGTACGGGCGGTCGAGAACGCACGG
GGTATCGTGATCCCTCCCAACGCCAGTATGGTTCGTAATATCATGCAAGCCGTATTGTACATGCATGACCATACGGTGCA
TTTCTATCAATTGCATGCATTGGATTGGGTAGATGTGGTATCCGCCTTAAAAGCCGATCCAAAAGCCGCCGCTTTACTTG
CCCAGCAACTTTCTCCATGGGCAAAGAATACCGAGGGGTATTTCACCGCCACTCAAGAACGACTAAAGAAGTTCGTTGCC
AGCGGGCAGCTTGGTATATTCGCCAATGGGTATTGGGGACATCCGGACTATAAACTTACGCCGGAACAGAACCTTATCGC
TACCGTCCATTATCTGGACGCATTGGAATGGCAGAAAGAGGTCGTCAAGGTACACGCTGTGTTCGGGGGCAAGAATCCGC
ATCCAAACTATATCGTAGGCGGTATGCCTTGCAGCATCGACTTAAATGAGGCCAATGCGATCAACGCAGATCGACTCGCT
CTGGTAAAGCAGAAACTAGAGGAGGCGAAAACTTTCATTAATCAAGTGTATATCCCAGATTTATTGATGATCGCCAACGT
CTATAAAGACAAATGGAGTAAGATTGGCGGAGGCGTACGAAACTATTTGTCTTACGGAGACTACCCGGTGTTCGATCTCG
GGGAAGTGGAAAGTTATAAGATCCCCAGAGGAATCGTCTTGGATCGTGACCTTAGCAAGGTACATCCCGTAGACGCTAAC
TCCCCGGAAGAAATCAAGGAGTACATTTATCATTCATGGTATAAATACACGCAAGGAGACAAAGCCGGTTTGCATCCCTA
CGAGGGAGAGACCCATCTGGAATATACTGGCCCTCGTCCCCCCTATAAGTTATTGGACGTAGAGGATAAATATAGTTGGA
TCAAGACGCCACGGTGGAAACAGGAACCAATGGAAGTGGGTCCGTTAGCCCGTTTGATCGTAGCTTACGCCGCAGGGAAG
GAACCTCAAAAGAGTATCGTAGACGAGACTTTACGTCAGCTTGACTTACCGGTAGATGCTTTATTCTCTACCCTAGGACG
TACCGCCGCTCGTGGATTGGAATGTAGAATGGCAGCAGACTGGGCCCTTGAGTTTTTCAACCAATTGATCAAGAACCTCG
AGAACGGGGATTCCCGGATGGCAAACAGTGCCGAATGGGAACGGGAGAACTGGCCCGATCATGAGCAAGGCGTAGGATTG
GCCGAAGCTCCCCGTGGAGCATTAGCCCATTTTATCGTGATCGACAAGAACCGGGTAAAGAACTACCAGATGGTAGTGCC
TACTACTTGGAACGCCTCACCGAGGGATGAGAACGGCGTATTGTCCGCCTACGAATCCGCTTTGATCGGTACGCCGATAT
ACGATCCGAAGCAACCCCTAGAGATTCTTCGGACGATCCACTCTTTCGATCCTTGTCTGGCTTGTGCCGTGCATCTGTAT
GACGAGGAAGGAAAATATGTGCACCAAGTTGATACATTCTAA

Upstream 100 bases:

>100_bases
TATCGCCGTACATGCCGTAGCAACTAACATACGGAAAAAAGAGCTTATCGACAATGATGAGCCGGAAAGTAAATCTACGA
TCTAATAAAACACTACTATC

Downstream 100 bases:

>100_bases
AAAACAATAGAGCCATGAAGAGTCGTAAGAAACGTTTAAGAGAAGTGTATGTATGGGAATTACCCGTACGTATCTATCAT
TGGGTGAATGCGCTCTGTAT

Product: stable NiFe hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSERIVIDPITRIEGHLRVEAEIEGGTIKNAYSSGTMVRGIENIVKDRDPRDVWAFVGRVCGVCTSIHSLASVRAVENAR
GIVIPPNASMVRNIMQAVLYMHDHTVHFYQLHALDWVDVVSALKADPKAAALLAQQLSPWAKNTEGYFTATQERLKKFVA
SGQLGIFANGYWGHPDYKLTPEQNLIATVHYLDALEWQKEVVKVHAVFGGKNPHPNYIVGGMPCSIDLNEANAINADRLA
LVKQKLEEAKTFINQVYIPDLLMIANVYKDKWSKIGGGVRNYLSYGDYPVFDLGEVESYKIPRGIVLDRDLSKVHPVDAN
SPEEIKEYIYHSWYKYTQGDKAGLHPYEGETHLEYTGPRPPYKLLDVEDKYSWIKTPRWKQEPMEVGPLARLIVAYAAGK
EPQKSIVDETLRQLDLPVDALFSTLGRTAARGLECRMAADWALEFFNQLIKNLENGDSRMANSAEWERENWPDHEQGVGL
AEAPRGALAHFIVIDKNRVKNYQMVVPTTWNASPRDENGVLSAYESALIGTPIYDPKQPLEILRTIHSFDPCLACAVHLY
DEEGKYVHQVDTF

Sequences:

>Translated_573_residues
MSERIVIDPITRIEGHLRVEAEIEGGTIKNAYSSGTMVRGIENIVKDRDPRDVWAFVGRVCGVCTSIHSLASVRAVENAR
GIVIPPNASMVRNIMQAVLYMHDHTVHFYQLHALDWVDVVSALKADPKAAALLAQQLSPWAKNTEGYFTATQERLKKFVA
SGQLGIFANGYWGHPDYKLTPEQNLIATVHYLDALEWQKEVVKVHAVFGGKNPHPNYIVGGMPCSIDLNEANAINADRLA
LVKQKLEEAKTFINQVYIPDLLMIANVYKDKWSKIGGGVRNYLSYGDYPVFDLGEVESYKIPRGIVLDRDLSKVHPVDAN
SPEEIKEYIYHSWYKYTQGDKAGLHPYEGETHLEYTGPRPPYKLLDVEDKYSWIKTPRWKQEPMEVGPLARLIVAYAAGK
EPQKSIVDETLRQLDLPVDALFSTLGRTAARGLECRMAADWALEFFNQLIKNLENGDSRMANSAEWERENWPDHEQGVGL
AEAPRGALAHFIVIDKNRVKNYQMVVPTTWNASPRDENGVLSAYESALIGTPIYDPKQPLEILRTIHSFDPCLACAVHLY
DEEGKYVHQVDTF
>Mature_572_residues
SERIVIDPITRIEGHLRVEAEIEGGTIKNAYSSGTMVRGIENIVKDRDPRDVWAFVGRVCGVCTSIHSLASVRAVENARG
IVIPPNASMVRNIMQAVLYMHDHTVHFYQLHALDWVDVVSALKADPKAAALLAQQLSPWAKNTEGYFTATQERLKKFVAS
GQLGIFANGYWGHPDYKLTPEQNLIATVHYLDALEWQKEVVKVHAVFGGKNPHPNYIVGGMPCSIDLNEANAINADRLAL
VKQKLEEAKTFINQVYIPDLLMIANVYKDKWSKIGGGVRNYLSYGDYPVFDLGEVESYKIPRGIVLDRDLSKVHPVDANS
PEEIKEYIYHSWYKYTQGDKAGLHPYEGETHLEYTGPRPPYKLLDVEDKYSWIKTPRWKQEPMEVGPLARLIVAYAAGKE
PQKSIVDETLRQLDLPVDALFSTLGRTAARGLECRMAADWALEFFNQLIKNLENGDSRMANSAEWERENWPDHEQGVGLA
EAPRGALAHFIVIDKNRVKNYQMVVPTTWNASPRDENGVLSAYESALIGTPIYDPKQPLEILRTIHSFDPCLACAVHLYD
EEGKYVHQVDTF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=577, Percent_Identity=51.1265164644714, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789368, Length=586, Percent_Identity=45.5631399317406, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 64586; Mature: 64455

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERIVIDPITRIEGHLRVEAEIEGGTIKNAYSSGTMVRGIENIVKDRDPRDVWAFVGRV
CCCCEEECCHHHCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
CGVCTSIHSLASVRAVENARGIVIPPNASMVRNIMQAVLYMHDHTVHFYQLHALDWVDVV
HHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHH
SALKADPKAAALLAQQLSPWAKNTEGYFTATQERLKKFVASGQLGIFANGYWGHPDYKLT
HHHCCCHHHHHHHHHHHCCHHCCCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCEEC
PEQNLIATVHYLDALEWQKEVVKVHAVFGGKNPHPNYIVGGMPCSIDLNEANAINADRLA
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCEEECCCCCCCCHHHHH
LVKQKLEEAKTFINQVYIPDLLMIANVYKDKWSKIGGGVRNYLSYGDYPVFDLGEVESYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEECCCCCCEE
IPRGIVLDRDLSKVHPVDANSPEEIKEYIYHSWYKYTQGDKAGLHPYEGETHLEYTGPRP
CCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
PYKLLDVEDKYSWIKTPRWKQEPMEVGPLARLIVAYAAGKEPQKSIVDETLRQLDLPVDA
CCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LFSTLGRTAARGLECRMAADWALEFFNQLIKNLENGDSRMANSAEWERENWPDHEQGVGL
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHCCCC
AEAPRGALAHFIVIDKNRVKNYQMVVPTTWNASPRDENGVLSAYESALIGTPIYDPKQPL
CCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
EILRTIHSFDPCLACAVHLYDEEGKYVHQVDTF
HHHHHHHCCCHHHHHHHHEECCCCCEEEECCCC
>Mature Secondary Structure 
SERIVIDPITRIEGHLRVEAEIEGGTIKNAYSSGTMVRGIENIVKDRDPRDVWAFVGRV
CCCEEECCHHHCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
CGVCTSIHSLASVRAVENARGIVIPPNASMVRNIMQAVLYMHDHTVHFYQLHALDWVDVV
HHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHH
SALKADPKAAALLAQQLSPWAKNTEGYFTATQERLKKFVASGQLGIFANGYWGHPDYKLT
HHHCCCHHHHHHHHHHHCCHHCCCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCEEC
PEQNLIATVHYLDALEWQKEVVKVHAVFGGKNPHPNYIVGGMPCSIDLNEANAINADRLA
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCEEECCCCCCCCHHHHH
LVKQKLEEAKTFINQVYIPDLLMIANVYKDKWSKIGGGVRNYLSYGDYPVFDLGEVESYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEECCCCCCEE
IPRGIVLDRDLSKVHPVDANSPEEIKEYIYHSWYKYTQGDKAGLHPYEGETHLEYTGPRP
CCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
PYKLLDVEDKYSWIKTPRWKQEPMEVGPLARLIVAYAAGKEPQKSIVDETLRQLDLPVDA
CCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LFSTLGRTAARGLECRMAADWALEFFNQLIKNLENGDSRMANSAEWERENWPDHEQGVGL
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHCCCC
AEAPRGALAHFIVIDKNRVKNYQMVVPTTWNASPRDENGVLSAYESALIGTPIYDPKQPL
CCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
EILRTIHSFDPCLACAVHLYDEEGKYVHQVDTF
HHHHHHHCCCHHHHHHHHEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2402452; 2103457 [H]