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The map label for this gene is hypF [H]

Identifier: 150008425

GI number: 150008425

Start: 2154929

End: 2157211

Strand: Reverse

Name: hypF [H]

Synonym: BDI_1807

Alternate gene names: 150008425

Gene position: 2157211-2154929 (Counterclockwise)

Preceding gene: 150008433

Following gene: 150008424

Centisome position: 44.84

GC content: 49.28

Gene sequence:

>2283_bases
ATGGCGAAGAATAAAGTATATGCGTTGATTATTCAAGGCTTGGTTCAAGGGGTTGGTTTTCGCCCTTTTATCTACCGGAT
CGCTAAAGATCTGGGGATGAAAGGTTGTGTGGAGAATATGAATAATGGAGTACGTATATTGGTTGCCGCTACTCCGGATG
ATCGGGATTTGCTGATAAGTCGTATACGGACCGAGCATCCTCGTGTCGCTTATATCCATCGGATCTCCTATACATCTACG
GAAATGGATGAAGATGATTTTGACGATTTTACGATTACGCCCAGCCATTCAGAATCGGATGAGGTCACGCAGGTCTCCCC
GGATATCGCTGTTTGTGCGGATTGTATGCGAGACCGGACTACGCAACCCCATCGAATCGGATATCCTTTTATTAATTGTA
CGCATTGTGGCCCTCGTTTCTCTATTATCCGGGACCTTCCTTACGACCGTTCGCAGACAACGATGGGCGGTTTCCTTATG
TGTCCCGATTGTGAGAAAGAATATACGAATGTTATCGATCGCCGTTTCCATGCCCAACCGGTCGCTTGCAATCATTGTGG
CCCGACTTATTACGCGACTTATAACGAGGAGACTTATATCGATTATGAGACCTTATTGAAACTTACCTCTCGTCTATTGC
TTGGTGGAGAGGTTATTGCGGCTAAAGGAATCGGAGGCTATCATTTGATTTGTGATGCCTCGAACGAGAGAGCGGTTGCC
CGTCTGCGAGAGATAAAGCAGCGGGATACCAAACCATTCGCTGTTATGTTCCGTGATCTGGAACATTTGCAAGTCTATAC
GGCAACGGAGCCGATGGAGGAGCGATGCCTCGTCTCTTGGCGCCGTCCGATCGTGCTGCTGAGGCAACGGAGCCGGTTAG
CTTCCGGAATCAATCCGGGCATGCATACGCTAGGATGTATGTTGTCTTATATGCCGATTCATTACGATTGGTTTGCTCGT
ACAGGGATACCTGCGTTGGTAATGACCAGCGGGAATCTAAGCGATTTGCCGATAGCGATCACCCCAGAGGATGCGGAGGC
GCAATTAGCGGGAAAGGTAGCGATCCTCTTGCATCATAATCGCCCGATCCATAACCGGGTAGATGATTCTGTGTTGCAGG
TCTGCGGAGGACAACCCTGCTTGATCCGTCGTTCCCGAGGCTATGTGCCGGAACCCTTTTTCACGGATACAAATGTGGAA
GGGATCATGGCTTTCGGTGCGGAGAAAGTGAACACGTTCGCTTTGGGAAAAGGAGAGACGATCCTGCAAAGTCAATATAT
TGGCGACTTAAAGAACTGGGAGACTTTCCGGTTCTATACGGAATCTATGGAGCGGTTTCGTCATTTGTTTCGTTTCAATC
TCCAGCGATTGGTTTGTGATCTGCATCCGGATTATCTTTCCAGCCAAGAGGCGGAACGTATCTCGAAATCTCTTTCCTTA
CCTTTATTAAAGGTACAACATCATCATGCTCATGCCGCTGCTTGTATGTTGGAGCATGGATTGAATGAGCCGGTTCTCGC
TATCGTGATGGATGGGACAGGTCTGGGTGATGATGGTAAGGTTTGGGGAGGCGAGTTCTTCTTTTGTGACCGAGCGAAAT
ACCGTCGCCTTTCCCATTTTGAGTATGTCCCGTTACCGGGAGGCGACAAGGCGGCTGAGGAGCCATGGCGAATGGTTGTC
GCCTATTTGTGGCATTATTTCAAGGACGAACCGTCGGGTATTCCTTATCCGGCCGATTTCGTGGAACGCATAGGCACGGA
AAGGATCGCTATGCTTGAACGAATGATGGAGAAAGGTGTGAATACACCCTATACATCCAGCGCTGGCCGGCTGTTTGATG
CGGTAGCTTCTTTATTGGGGATTTGCGATGTCTCCAGCCACCAAGCGGAGGCTCCGGTCTTATTGGAGCAGGCGGCGATG
GGAGAGAGGAACGCTTATGCGTATCCAGTCTCGGCGGAGGGAGAGGAAATCTCTTTTTATTCTTTGTTTGAGGCATTATT
GCATGATAAGACGAACGAGGTCCCGGTGAGCTTGATATCCGCCCGTTTCCATACGACATTGGCTTCCTTGTTTGTGCAGA
AAGCCCGCTTGTTGATTAAACGGACGAAAGCGACACAAGTGGTTATCTCGGGAGGATGTTTCCAGAATAAATTATTGACG
GAGTCGATGAGGAGACAGTTCATCATGGCTGGGGTCCCCGTCTATATTCCGTCTCGTATACCTTGTAACGATGGGGGGAT
AGCCGTGGGGCAATTAACGATCGCCTCGGTGACTCCCTTTTAA

Upstream 100 bases:

>100_bases
AAATTGATAAATATTATTTATAAGATGATAGGTAATATCTCTTTAGTAAATACGATAGTCGATTCGTGTCTGATTAGACA
TTTATTCCTATATTTGTGCT

Downstream 100 bases:

>100_bases
AAGAGATCAGATATGCATGAGTTGTCTATAGCCCGTAGCATCGTGGAGCTGGTCGAGGAACAAGCGGATAACCGGGGAGC
CTCGGTGGTCGAAGAGTTGG

Product: hydrogenase maturation factor

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MAKNKVYALIIQGLVQGVGFRPFIYRIAKDLGMKGCVENMNNGVRILVAATPDDRDLLISRIRTEHPRVAYIHRISYTST
EMDEDDFDDFTITPSHSESDEVTQVSPDIAVCADCMRDRTTQPHRIGYPFINCTHCGPRFSIIRDLPYDRSQTTMGGFLM
CPDCEKEYTNVIDRRFHAQPVACNHCGPTYYATYNEETYIDYETLLKLTSRLLLGGEVIAAKGIGGYHLICDASNERAVA
RLREIKQRDTKPFAVMFRDLEHLQVYTATEPMEERCLVSWRRPIVLLRQRSRLASGINPGMHTLGCMLSYMPIHYDWFAR
TGIPALVMTSGNLSDLPIAITPEDAEAQLAGKVAILLHHNRPIHNRVDDSVLQVCGGQPCLIRRSRGYVPEPFFTDTNVE
GIMAFGAEKVNTFALGKGETILQSQYIGDLKNWETFRFYTESMERFRHLFRFNLQRLVCDLHPDYLSSQEAERISKSLSL
PLLKVQHHHAHAAACMLEHGLNEPVLAIVMDGTGLGDDGKVWGGEFFFCDRAKYRRLSHFEYVPLPGGDKAAEEPWRMVV
AYLWHYFKDEPSGIPYPADFVERIGTERIAMLERMMEKGVNTPYTSSAGRLFDAVASLLGICDVSSHQAEAPVLLEQAAM
GERNAYAYPVSAEGEEISFYSLFEALLHDKTNEVPVSLISARFHTTLASLFVQKARLLIKRTKATQVVISGGCFQNKLLT
ESMRRQFIMAGVPVYIPSRIPCNDGGIAVGQLTIASVTPF

Sequences:

>Translated_760_residues
MAKNKVYALIIQGLVQGVGFRPFIYRIAKDLGMKGCVENMNNGVRILVAATPDDRDLLISRIRTEHPRVAYIHRISYTST
EMDEDDFDDFTITPSHSESDEVTQVSPDIAVCADCMRDRTTQPHRIGYPFINCTHCGPRFSIIRDLPYDRSQTTMGGFLM
CPDCEKEYTNVIDRRFHAQPVACNHCGPTYYATYNEETYIDYETLLKLTSRLLLGGEVIAAKGIGGYHLICDASNERAVA
RLREIKQRDTKPFAVMFRDLEHLQVYTATEPMEERCLVSWRRPIVLLRQRSRLASGINPGMHTLGCMLSYMPIHYDWFAR
TGIPALVMTSGNLSDLPIAITPEDAEAQLAGKVAILLHHNRPIHNRVDDSVLQVCGGQPCLIRRSRGYVPEPFFTDTNVE
GIMAFGAEKVNTFALGKGETILQSQYIGDLKNWETFRFYTESMERFRHLFRFNLQRLVCDLHPDYLSSQEAERISKSLSL
PLLKVQHHHAHAAACMLEHGLNEPVLAIVMDGTGLGDDGKVWGGEFFFCDRAKYRRLSHFEYVPLPGGDKAAEEPWRMVV
AYLWHYFKDEPSGIPYPADFVERIGTERIAMLERMMEKGVNTPYTSSAGRLFDAVASLLGICDVSSHQAEAPVLLEQAAM
GERNAYAYPVSAEGEEISFYSLFEALLHDKTNEVPVSLISARFHTTLASLFVQKARLLIKRTKATQVVISGGCFQNKLLT
ESMRRQFIMAGVPVYIPSRIPCNDGGIAVGQLTIASVTPF
>Mature_759_residues
AKNKVYALIIQGLVQGVGFRPFIYRIAKDLGMKGCVENMNNGVRILVAATPDDRDLLISRIRTEHPRVAYIHRISYTSTE
MDEDDFDDFTITPSHSESDEVTQVSPDIAVCADCMRDRTTQPHRIGYPFINCTHCGPRFSIIRDLPYDRSQTTMGGFLMC
PDCEKEYTNVIDRRFHAQPVACNHCGPTYYATYNEETYIDYETLLKLTSRLLLGGEVIAAKGIGGYHLICDASNERAVAR
LREIKQRDTKPFAVMFRDLEHLQVYTATEPMEERCLVSWRRPIVLLRQRSRLASGINPGMHTLGCMLSYMPIHYDWFART
GIPALVMTSGNLSDLPIAITPEDAEAQLAGKVAILLHHNRPIHNRVDDSVLQVCGGQPCLIRRSRGYVPEPFFTDTNVEG
IMAFGAEKVNTFALGKGETILQSQYIGDLKNWETFRFYTESMERFRHLFRFNLQRLVCDLHPDYLSSQEAERISKSLSLP
LLKVQHHHAHAAACMLEHGLNEPVLAIVMDGTGLGDDGKVWGGEFFFCDRAKYRRLSHFEYVPLPGGDKAAEEPWRMVVA
YLWHYFKDEPSGIPYPADFVERIGTERIAMLERMMEKGVNTPYTSSAGRLFDAVASLLGICDVSSHQAEAPVLLEQAAMG
ERNAYAYPVSAEGEEISFYSLFEALLHDKTNEVPVSLISARFHTTLASLFVQKARLLIKRTKATQVVISGGCFQNKLLTE
SMRRQFIMAGVPVYIPSRIPCNDGGIAVGQLTIASVTPF

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=778, Percent_Identity=35.9897172236504, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 85570; Mature: 85439

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKVYALIIQGLVQGVGFRPFIYRIAKDLGMKGCVENMNNGVRILVAATPDDRDLLIS
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCHHHHHH
RIRTEHPRVAYIHRISYTSTEMDEDDFDDFTITPSHSESDEVTQVSPDIAVCADCMRDRT
HHHCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEECCCHHHHHHHHHHCCC
TQPHRIGYPFINCTHCGPRFSIIRDLPYDRSQTTMGGFLMCPDCEKEYTNVIDRRFHAQP
CCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
VACNHCGPTYYATYNEETYIDYETLLKLTSRLLLGGEVIAAKGIGGYHLICDASNERAVA
CEECCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCHHHH
RLREIKQRDTKPFAVMFRDLEHLQVYTATEPMEERCLVSWRRPIVLLRQRSRLASGINPG
HHHHHHHHCCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
MHTLGCMLSYMPIHYDWFARTGIPALVMTSGNLSDLPIAITPEDAEAQLAGKVAILLHHN
HHHHHHHHHHCCCEEEHHHHCCCCEEEEECCCCCCCCEEECCCCCCHHHCCEEEEEEECC
RPIHNRVDDSVLQVCGGQPCLIRRSRGYVPEPFFTDTNVEGIMAFGAEKVNTFALGKGET
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHEEECCHHCCEEECCCCCH
ILQSQYIGDLKNWETFRFYTESMERFRHLFRFNLQRLVCDLHPDYLSSQEAERISKSLSL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCC
PLLKVQHHHAHAAACMLEHGLNEPVLAIVMDGTGLGDDGKVWGGEFFFCDRAKYRRLSHF
CEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEECCEEEEECCHHHHHCCCC
EYVPLPGGDKAAEEPWRMVVAYLWHYFKDEPSGIPYPADFVERIGTERIAMLERMMEKGV
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCC
NTPYTSSAGRLFDAVASLLGICDVSSHQAEAPVLLEQAAMGERNAYAYPVSAEGEEISFY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHH
SLFEALLHDKTNEVPVSLISARFHTTLASLFVQKARLLIKRTKATQVVISGGCFQNKLLT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
ESMRRQFIMAGVPVYIPSRIPCNDGGIAVGQLTIASVTPF
HHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCC
>Mature Secondary Structure 
AKNKVYALIIQGLVQGVGFRPFIYRIAKDLGMKGCVENMNNGVRILVAATPDDRDLLIS
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCHHHHHH
RIRTEHPRVAYIHRISYTSTEMDEDDFDDFTITPSHSESDEVTQVSPDIAVCADCMRDRT
HHHCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEECCCHHHHHHHHHHCCC
TQPHRIGYPFINCTHCGPRFSIIRDLPYDRSQTTMGGFLMCPDCEKEYTNVIDRRFHAQP
CCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
VACNHCGPTYYATYNEETYIDYETLLKLTSRLLLGGEVIAAKGIGGYHLICDASNERAVA
CEECCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCHHHH
RLREIKQRDTKPFAVMFRDLEHLQVYTATEPMEERCLVSWRRPIVLLRQRSRLASGINPG
HHHHHHHHCCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
MHTLGCMLSYMPIHYDWFARTGIPALVMTSGNLSDLPIAITPEDAEAQLAGKVAILLHHN
HHHHHHHHHHCCCEEEHHHHCCCCEEEEECCCCCCCCEEECCCCCCHHHCCEEEEEEECC
RPIHNRVDDSVLQVCGGQPCLIRRSRGYVPEPFFTDTNVEGIMAFGAEKVNTFALGKGET
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHEEECCHHCCEEECCCCCH
ILQSQYIGDLKNWETFRFYTESMERFRHLFRFNLQRLVCDLHPDYLSSQEAERISKSLSL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCC
PLLKVQHHHAHAAACMLEHGLNEPVLAIVMDGTGLGDDGKVWGGEFFFCDRAKYRRLSHF
CEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEECCEEEEECCHHHHHCCCC
EYVPLPGGDKAAEEPWRMVVAYLWHYFKDEPSGIPYPADFVERIGTERIAMLERMMEKGV
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCC
NTPYTSSAGRLFDAVASLLGICDVSSHQAEAPVLLEQAAMGERNAYAYPVSAEGEEISFY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHH
SLFEALLHDKTNEVPVSLISARFHTTLASLFVQKARLLIKRTKATQVVISGGCFQNKLLT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
ESMRRQFIMAGVPVYIPSRIPCNDGGIAVGQLTIASVTPF
HHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190; 9492269 [H]