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The map label for this gene is metF [H]

Identifier: 150008401

GI number: 150008401

Start: 2117336

End: 2118289

Strand: Direct

Name: metF [H]

Synonym: BDI_1777

Alternate gene names: 150008401

Gene position: 2117336-2118289 (Clockwise)

Preceding gene: 150008397

Following gene: 150008402

Centisome position: 44.01

GC content: 42.98

Gene sequence:

>954_bases
ATGAAAGTCATTGATCTGATAAGGAATAGTAAGAGTACCGCTTTTTCTTTCGAGATATTACCGCCGCTGAAAGGTAACAG
TATCCAGAAGGTATATAATGTGATTGACAAGTTGAAGGAATTCGACCCTAAATACATAAATATCACCTCCCATCATAGCG
AGTTCGTCTATAAAACCTTACCAGACGGAAGTTTCCAGAAAGTAAACATCCGTAAACGTCCGGGCTCCGTAGCGATCGCT
TCGGCTATCCAGAATAAATACGGTATTCCTGCCGTACCCCATATTATATGTAAAGGCTTTACGAAAGACGAGACGGAATA
CGCTTTGATCGATTTGAACTTCCTTGGCGTAAACAACCTCTTAATATTAAGGGGAGATATAAAGACCTTAGAGGCCTCCC
AGCAAAAACCGGAGTTATATCACGACCACGCAACTGATTTACAACAGCAGGTAAATCATTTCAACGAAGGTATTGCGTTA
GACGGTTCCAAGATAGACGGTATAGAGACTCCCTTCTCGTACGGCATGGCTTGCTATCCGGAGAAACACGAGGAAGCACC
CAATATGGAATCCGATATCTATTACTTAAAGGAGAAAGTAAAAAATGGCGCTGAATATCTGGTAACCCAGATGTTTTTCG
ATAATGACAAGTACTATGCGTTCGTGGATCGTTGCCGTGCGGAAGGTATCACGGTTCCCATCATCCCCGGTATCAAACCG
ATCGTTTTCAAGAACCAATTAACGGTTCTTCCGAAGATATTCCGTGCGGATATCCCAGAGCCTTTCGCGACAGAATTACG
TAAATGTAAAGATGATGCGGAAGCGAAAGAGGTGGGTGTGGAATGGTGTATACAGCAGTGCAAGGATCTGATCGCTCATG
GCGTACCGAGCTTACATTTCTACACAATGATGGCCTCGGATAGCGTTTACAAAATAGCAAAAGAAATTTATTGA

Upstream 100 bases:

>100_bases
ACACGAAAGCGCTCAGTTAGCCGCTTTGTTAGATTAATGATCGTAAATGTAAGCTATTTCGAAGTATATTTGCTACATTC
GCGTCAAAAATAATTAAAAT

Downstream 100 bases:

>100_bases
GATACAATAGGGGTTTTTCCACGCTGAGTTGTCTAATAGAAGTACACGATTGAACAATCATGTACTAAGTATGGTTATTT
TTAAAATTCTAAGGTAATGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKVIDLIRNSKSTAFSFEILPPLKGNSIQKVYNVIDKLKEFDPKYINITSHHSEFVYKTLPDGSFQKVNIRKRPGSVAIA
SAIQNKYGIPAVPHIICKGFTKDETEYALIDLNFLGVNNLLILRGDIKTLEASQQKPELYHDHATDLQQQVNHFNEGIAL
DGSKIDGIETPFSYGMACYPEKHEEAPNMESDIYYLKEKVKNGAEYLVTQMFFDNDKYYAFVDRCRAEGITVPIIPGIKP
IVFKNQLTVLPKIFRADIPEPFATELRKCKDDAEAKEVGVEWCIQQCKDLIAHGVPSLHFYTMMASDSVYKIAKEIY

Sequences:

>Translated_317_residues
MKVIDLIRNSKSTAFSFEILPPLKGNSIQKVYNVIDKLKEFDPKYINITSHHSEFVYKTLPDGSFQKVNIRKRPGSVAIA
SAIQNKYGIPAVPHIICKGFTKDETEYALIDLNFLGVNNLLILRGDIKTLEASQQKPELYHDHATDLQQQVNHFNEGIAL
DGSKIDGIETPFSYGMACYPEKHEEAPNMESDIYYLKEKVKNGAEYLVTQMFFDNDKYYAFVDRCRAEGITVPIIPGIKP
IVFKNQLTVLPKIFRADIPEPFATELRKCKDDAEAKEVGVEWCIQQCKDLIAHGVPSLHFYTMMASDSVYKIAKEIY
>Mature_317_residues
MKVIDLIRNSKSTAFSFEILPPLKGNSIQKVYNVIDKLKEFDPKYINITSHHSEFVYKTLPDGSFQKVNIRKRPGSVAIA
SAIQNKYGIPAVPHIICKGFTKDETEYALIDLNFLGVNNLLILRGDIKTLEASQQKPELYHDHATDLQQQVNHFNEGIAL
DGSKIDGIETPFSYGMACYPEKHEEAPNMESDIYYLKEKVKNGAEYLVTQMFFDNDKYYAFVDRCRAEGITVPIIPGIKP
IVFKNQLTVLPKIFRADIPEPFATELRKCKDDAEAKEVGVEWCIQQCKDLIAHGVPSLHFYTMMASDSVYKIAKEIY

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=308, Percent_Identity=31.1688311688312, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1790377, Length=298, Percent_Identity=30.8724832214765, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25147441, Length=318, Percent_Identity=28.9308176100629, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25147436, Length=318, Percent_Identity=28.9308176100629, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=312, Percent_Identity=27.2435897435897, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325234, Length=333, Percent_Identity=27.027027027027, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36039; Mature: 36039

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIDLIRNSKSTAFSFEILPPLKGNSIQKVYNVIDKLKEFDPKYINITSHHSEFVYKTL
CCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEC
PDGSFQKVNIRKRPGSVAIASAIQNKYGIPAVPHIICKGFTKDETEYALIDLNFLGVNNL
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCE
LILRGDIKTLEASQQKPELYHDHATDLQQQVNHFNEGIALDGSKIDGIETPFSYGMACYP
EEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHCCEECCC
EKHEEAPNMESDIYYLKEKVKNGAEYLVTQMFFDNDKYYAFVDRCRAEGITVPIIPGIKP
CHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHCCCEEEECCCCCC
IVFKNQLTVLPKIFRADIPEPFATELRKCKDDAEAKEVGVEWCIQQCKDLIAHGVPSLHF
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YTMMASDSVYKIAKEIY
EEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKVIDLIRNSKSTAFSFEILPPLKGNSIQKVYNVIDKLKEFDPKYINITSHHSEFVYKTL
CCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEC
PDGSFQKVNIRKRPGSVAIASAIQNKYGIPAVPHIICKGFTKDETEYALIDLNFLGVNNL
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCE
LILRGDIKTLEASQQKPELYHDHATDLQQQVNHFNEGIALDGSKIDGIETPFSYGMACYP
EEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHCCEECCC
EKHEEAPNMESDIYYLKEKVKNGAEYLVTQMFFDNDKYYAFVDRCRAEGITVPIIPGIKP
CHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHCCCEEEECCCCCC
IVFKNQLTVLPKIFRADIPEPFATELRKCKDDAEAKEVGVEWCIQQCKDLIAHGVPSLHF
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YTMMASDSVYKIAKEIY
EEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]