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The map label for this gene is dnaX [C]

Identifier: 150008402

GI number: 150008402

Start: 2118544

End: 2119668

Strand: Direct

Name: dnaX [C]

Synonym: BDI_1778

Alternate gene names: 150008402

Gene position: 2118544-2119668 (Clockwise)

Preceding gene: 150008401

Following gene: 150008404

Centisome position: 44.03

GC content: 47.91

Gene sequence:

>1125_bases
ATGTATTTTAAAGATATCATCGGGCAAGAGGAAGTAAAGGAACGGTTGAGGCAATCGACTCGGACAGGGATCGTACCCCA
TGCGCAGTTATTCACGGAGCAAGGGGGGGCCGGGGCGTTTCCGCTGGCTTTGGCTTACGCCCGTTATCTGAATTGTACGA
ACCGGACGGAGACGGACGCTTGCGGACGTTGCCCGTCATGCCTTAAATATGACGAATTGGCACATCCCGATTTGCATTTC
GTATTCCCAATCGTAGCCAAGAAAGAGAAAAAGAAAGAGGTCTGCGATGATTATCTCAGTGAATGGCGTGGGTTCCTCAA
AGAACATCCCTATTTCAATATGGATGAATGGCTGGATTATATCGAGGCCGGAAACTCACAAGCGATCATCTATTCCAAGG
AAAGCGATGAGATTATCCATAAACTGAGCCTAAAGATTTATGAGGCGGAATATCGTATCCTGATGGTTTGGTTGCCGGAG
AAACTACATCCTACTTGCGCCAACAAGCTTCTGAAAATCATAGAGGAGCCTCCTATGAAAACGGTTATCCTTATGGTATC
CGAGACACCGGACCTTATCCTCGGGACCATCCAATCCCGTGCGCAACGCATCCACATCCGTCCCATTGAGACCGATGCGA
TCATGAATGCCATGGTATCCCGTTTCGGGCTTTCGCCCGACGACGCTAAACACGTCGCTCACCTCTCCTCCGGAAGTTTT
ATCAAGGCGATGGAGGCGATTAGCTTGGGCGAGGAAAATAAATTCTTTCTGGAGCAATTCAAGGCCATGATGCGAAACTC
ATGGGCACGAAACGTAAAAGGCATGAAAGCGATGGCGGACGTCATGGCAGGGATCGGACGGGAACGACAAAAAAACTTCC
TCTCCTATTGTCAGCATTTGATCCGGGAGAACTTCATGTACCGGTTCCAATCGCCGGAACTTAATTATATGAATCTGGAC
GAGGCTAGCTTCTCCGTTAAGTTCTCCCCTTTCGTCAACGAGCGAAACGTGATCGATTTGATGGAAGAATTGGCGAAAGC
GGAACGCCATATCACACAGAACGTGAATGCCAAGATGGTCTTTTTTGATTTATCCTTACGAATTACCGTACTAATCAAAA
GATAA

Upstream 100 bases:

>100_bases
TCTATATATGTCAATTGATATTTACAAAACCCCGCACCTTTTGATATCTGAATACTTTTCCGTAACTTCGTTCCTTATTC
TATAGAAAAGGAAATATCTC

Downstream 100 bases:

>100_bases
GTCTTCAGATTTTCTTGTTACGCCAATTTCCTTTCCATAGATAGAAGAAAGAAGAAAGGATCATGATATTATATACATAC
TCCGTAGTCCAGCAAACCGC

Product: putative DNA polymerase III subunit delta

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Delta Subunit; DNA Polymerase III Subunit Delta; DNA-Directed DNA Polymerase; DNA Polymerase III Gamma/Tau Subunit; DNA Polymerase III Gamma/Tau Subunits-Like Protein; DNA Polymerase III Gamma And Tau Subunit Protein; DNA Polymerase III Gamma/Tau Subunits; DNA Polymerase III Subunits Gamma And Tau; DNA Polymerase III Delta&Apos; DNA-Directed DNA Polymerase Delta Prime Subunit; DNA Polymerase III Gamma And Tau Subunit; DNA Polymerase III Delta Subunit HolB

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MYFKDIIGQEEVKERLRQSTRTGIVPHAQLFTEQGGAGAFPLALAYARYLNCTNRTETDACGRCPSCLKYDELAHPDLHF
VFPIVAKKEKKKEVCDDYLSEWRGFLKEHPYFNMDEWLDYIEAGNSQAIIYSKESDEIIHKLSLKIYEAEYRILMVWLPE
KLHPTCANKLLKIIEEPPMKTVILMVSETPDLILGTIQSRAQRIHIRPIETDAIMNAMVSRFGLSPDDAKHVAHLSSGSF
IKAMEAISLGEENKFFLEQFKAMMRNSWARNVKGMKAMADVMAGIGRERQKNFLSYCQHLIRENFMYRFQSPELNYMNLD
EASFSVKFSPFVNERNVIDLMEELAKAERHITQNVNAKMVFFDLSLRITVLIKR

Sequences:

>Translated_374_residues
MYFKDIIGQEEVKERLRQSTRTGIVPHAQLFTEQGGAGAFPLALAYARYLNCTNRTETDACGRCPSCLKYDELAHPDLHF
VFPIVAKKEKKKEVCDDYLSEWRGFLKEHPYFNMDEWLDYIEAGNSQAIIYSKESDEIIHKLSLKIYEAEYRILMVWLPE
KLHPTCANKLLKIIEEPPMKTVILMVSETPDLILGTIQSRAQRIHIRPIETDAIMNAMVSRFGLSPDDAKHVAHLSSGSF
IKAMEAISLGEENKFFLEQFKAMMRNSWARNVKGMKAMADVMAGIGRERQKNFLSYCQHLIRENFMYRFQSPELNYMNLD
EASFSVKFSPFVNERNVIDLMEELAKAERHITQNVNAKMVFFDLSLRITVLIKR
>Mature_374_residues
MYFKDIIGQEEVKERLRQSTRTGIVPHAQLFTEQGGAGAFPLALAYARYLNCTNRTETDACGRCPSCLKYDELAHPDLHF
VFPIVAKKEKKKEVCDDYLSEWRGFLKEHPYFNMDEWLDYIEAGNSQAIIYSKESDEIIHKLSLKIYEAEYRILMVWLPE
KLHPTCANKLLKIIEEPPMKTVILMVSETPDLILGTIQSRAQRIHIRPIETDAIMNAMVSRFGLSPDDAKHVAHLSSGSF
IKAMEAISLGEENKFFLEQFKAMMRNSWARNVKGMKAMADVMAGIGRERQKNFLSYCQHLIRENFMYRFQSPELNYMNLD
EASFSVKFSPFVNERNVIDLMEELAKAERHITQNVNAKMVFFDLSLRITVLIKR

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 43285; Mature: 43285

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKDIIGQEEVKERLRQSTRTGIVPHAQLFTEQGGAGAFPLALAYARYLNCTNRTETDA
CCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC
CGRCPSCLKYDELAHPDLHFVFPIVAKKEKKKEVCDDYLSEWRGFLKEHPYFNMDEWLDY
CCCCCCCCCHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IEAGNSQAIIYSKESDEIIHKLSLKIYEAEYRILMVWLPEKLHPTCANKLLKIIEEPPMK
HHCCCCEEEEEECCHHHHHHHHHHHEEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCE
TVILMVSETPDLILGTIQSRAQRIHIRPIETDAIMNAMVSRFGLSPDDAKHVAHLSSGSF
EEEEEEECCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
IKAMEAISLGEENKFFLEQFKAMMRNSWARNVKGMKAMADVMAGIGRERQKNFLSYCQHL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRENFMYRFQSPELNYMNLDEASFSVKFSPFVNERNVIDLMEELAKAERHITQNVNAKMV
HHHHHHEEECCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
FFDLSLRITVLIKR
EEEEEEEEEEEEEC
>Mature Secondary Structure
MYFKDIIGQEEVKERLRQSTRTGIVPHAQLFTEQGGAGAFPLALAYARYLNCTNRTETDA
CCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC
CGRCPSCLKYDELAHPDLHFVFPIVAKKEKKKEVCDDYLSEWRGFLKEHPYFNMDEWLDY
CCCCCCCCCHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IEAGNSQAIIYSKESDEIIHKLSLKIYEAEYRILMVWLPEKLHPTCANKLLKIIEEPPMK
HHCCCCEEEEEECCHHHHHHHHHHHEEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCE
TVILMVSETPDLILGTIQSRAQRIHIRPIETDAIMNAMVSRFGLSPDDAKHVAHLSSGSF
EEEEEEECCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
IKAMEAISLGEENKFFLEQFKAMMRNSWARNVKGMKAMADVMAGIGRERQKNFLSYCQHL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRENFMYRFQSPELNYMNLDEASFSVKFSPFVNERNVIDLMEELAKAERHITQNVNAKMV
HHHHHHEEECCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
FFDLSLRITVLIKR
EEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA