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The map label for this gene is radA [H]

Identifier: 150008380

GI number: 150008380

Start: 2081222

End: 2082592

Strand: Reverse

Name: radA [H]

Synonym: BDI_1754

Alternate gene names: 150008380

Gene position: 2082592-2081222 (Counterclockwise)

Preceding gene: 150008381

Following gene: 150008379

Centisome position: 43.28

GC content: 50.18

Gene sequence:

>1371_bases
ATGGCGAAAACAAAAACCGTATATGTCTGCTCTAACTGTGGCGCTGATTCCCCGAAATGGCTGGGTAAATGCCCGAATTG
TGGGGAGTGGAACACCTATGTAGAGGAGATCGTAACAAAGGAGCTTGCCGTGAAGCGGCCAGTTCCCGGTATTATGGAGG
GCAATAAGATCCGTCCGGTGTTATTGCGTGATATAACGACGGAAGAGGAGGCCCGTATCGATTTAAAAGATGATGAATTA
AATAGGGTCCTCGGCGGAGGCTTGGTGAAAGGCTCTTTGGTCTTGATCGGGGGAGAGCCGGGAATTGGAAAATCCACCTT
GGTCTTGCAAACCGTGTTGGGCTTGACAGGCTTGAAAACCCTATATGTCTCGGGCGAGGAGAGCAGTCGCCAGTTGAAAC
TCCGGGCGGACCGATTATCGCATGATAATCCGAACTGTTTCATCCTATGCGAGACGCATCTGGAACAGATTTTTACGCAA
GCGGCCAATATCCAGCCGGACTTGATGATCATAGATTCCATACAAACCATCTTTACGGAAGTTGTGGAATCTTCCCCCGG
CAGTGTTTCCCAAGTACGTGAGTGTAGTGCCGCTATCTTGAAATACGCCAAGGAAAGCGGTGTCCCGGTCTTATTGATCG
GCCATATCAATAAGGAAGGCAGTATCGCCGGTCCGAAGGTATTGGAGCATATCGTGGATACGGTGTTGCAGTTTGAGGGC
GATCAGCATTATATGTATCGTATCCTGCGGAGCATCAAGAACCGTTTTGGCAGTACGGCGGAACTAGGGATTTACGAGAT
GCGCCAGAACGGGCTTCGTGAGGTCAGCAATCCATCCGAGTTATTATTAACCCAGAATCATGAGGGACTGAGCGGTGTCG
CCATCGCCGCCGCTATCGAGGGGGTACGTCCTTTCTTGATCGAGACGCAAGCCTTGGTCAGCTCGGCGGTTTACGGTACG
CCGCAACGTAGCGCCACCGGATTCGATTTACGCCGAATGAATATGTTATTGGCGGTATTGGAGAAAAGGGCGGGTTTTAA
GTTGATCCAGAAAGATGTTTTCCTGAATATCGCCGGAGGTTTGAAAGTGAATGATCCGGCTATCGATTTGGCGGTAATCA
GCTCCATCCTTTCCTCTAGCTTGGATATCAGCATCGAGCCGGGTGTTTGTATGGCTGGCGAGGTGGGCTTGTCCGGAGAG
ATCCGTCCGGTCAACCGTATCGAGCAACGTATCTTGGAGGCGGAGAAGCTGGGTTTCTCCCGGATTATCATTCCGCATAA
TAATCTAAAAGGTTTCGATACGGCGAAGAGCAAGATTCAAATCGTACAGGTAAAGAAAGTGGAGGAAGCCTTTCGGCAAC
TATTCGGTTAA

Upstream 100 bases:

>100_bases
AGGTAGCCCGGAAATTAAGAAGGGCTGCGGTATCGGAAAGAAGATCGTTGTAGTAGGGGAAAAGTAGTTTTTTGTATCTT
TACGCCAAATTCAAGATAGA

Downstream 100 bases:

>100_bases
TATATAATAAACAATGATAAAAGAATTACAACTCAGGATATTGCCGGAAGAGGCTGTTAATGAGCAATCGCTAAGGCAAG
TAGTAGCCCGTGAGACGGGT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAKTKTVYVCSNCGADSPKWLGKCPNCGEWNTYVEEIVTKELAVKRPVPGIMEGNKIRPVLLRDITTEEEARIDLKDDEL
NRVLGGGLVKGSLVLIGGEPGIGKSTLVLQTVLGLTGLKTLYVSGEESSRQLKLRADRLSHDNPNCFILCETHLEQIFTQ
AANIQPDLMIIDSIQTIFTEVVESSPGSVSQVRECSAAILKYAKESGVPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEG
DQHYMYRILRSIKNRFGSTAELGIYEMRQNGLREVSNPSELLLTQNHEGLSGVAIAAAIEGVRPFLIETQALVSSAVYGT
PQRSATGFDLRRMNMLLAVLEKRAGFKLIQKDVFLNIAGGLKVNDPAIDLAVISSILSSSLDISIEPGVCMAGEVGLSGE
IRPVNRIEQRILEAEKLGFSRIIIPHNNLKGFDTAKSKIQIVQVKKVEEAFRQLFG

Sequences:

>Translated_456_residues
MAKTKTVYVCSNCGADSPKWLGKCPNCGEWNTYVEEIVTKELAVKRPVPGIMEGNKIRPVLLRDITTEEEARIDLKDDEL
NRVLGGGLVKGSLVLIGGEPGIGKSTLVLQTVLGLTGLKTLYVSGEESSRQLKLRADRLSHDNPNCFILCETHLEQIFTQ
AANIQPDLMIIDSIQTIFTEVVESSPGSVSQVRECSAAILKYAKESGVPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEG
DQHYMYRILRSIKNRFGSTAELGIYEMRQNGLREVSNPSELLLTQNHEGLSGVAIAAAIEGVRPFLIETQALVSSAVYGT
PQRSATGFDLRRMNMLLAVLEKRAGFKLIQKDVFLNIAGGLKVNDPAIDLAVISSILSSSLDISIEPGVCMAGEVGLSGE
IRPVNRIEQRILEAEKLGFSRIIIPHNNLKGFDTAKSKIQIVQVKKVEEAFRQLFG
>Mature_455_residues
AKTKTVYVCSNCGADSPKWLGKCPNCGEWNTYVEEIVTKELAVKRPVPGIMEGNKIRPVLLRDITTEEEARIDLKDDELN
RVLGGGLVKGSLVLIGGEPGIGKSTLVLQTVLGLTGLKTLYVSGEESSRQLKLRADRLSHDNPNCFILCETHLEQIFTQA
ANIQPDLMIIDSIQTIFTEVVESSPGSVSQVRECSAAILKYAKESGVPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEGD
QHYMYRILRSIKNRFGSTAELGIYEMRQNGLREVSNPSELLLTQNHEGLSGVAIAAAIEGVRPFLIETQALVSSAVYGTP
QRSATGFDLRRMNMLLAVLEKRAGFKLIQKDVFLNIAGGLKVNDPAIDLAVISSILSSSLDISIEPGVCMAGEVGLSGEI
RPVNRIEQRILEAEKLGFSRIIIPHNNLKGFDTAKSKIQIVQVKKVEEAFRQLFG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=456, Percent_Identity=45.3947368421053, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49844; Mature: 49713

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTKTVYVCSNCGADSPKWLGKCPNCGEWNTYVEEIVTKELAVKRPVPGIMEGNKIRPV
CCCCEEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LLRDITTEEEARIDLKDDELNRVLGGGLVKGSLVLIGGEPGIGKSTLVLQTVLGLTGLKT
EEECCCCCCCCEECCCHHHHHHHHCCCEEECEEEEEECCCCCCCHHHHHHHHHHHHCCEE
LYVSGEESSRQLKLRADRLSHDNPNCFILCETHLEQIFTQAANIQPDLMIIDSIQTIFTE
EEECCCCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
VVESSPGSVSQVRECSAAILKYAKESGVPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEG
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
DQHYMYRILRSIKNRFGSTAELGIYEMRQNGLREVSNPSELLLTQNHEGLSGVAIAAAIE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
GVRPFLIETQALVSSAVYGTPQRSATGFDLRRMNMLLAVLEKRAGFKLIQKDVFLNIAGG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEEEEECC
LKVNDPAIDLAVISSILSSSLDISIEPGVCMAGEVGLSGEIRPVNRIEQRILEAEKLGFS
CEECCCHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCE
RIIIPHNNLKGFDTAKSKIQIVQVKKVEEAFRQLFG
EEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKTKTVYVCSNCGADSPKWLGKCPNCGEWNTYVEEIVTKELAVKRPVPGIMEGNKIRPV
CCCEEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LLRDITTEEEARIDLKDDELNRVLGGGLVKGSLVLIGGEPGIGKSTLVLQTVLGLTGLKT
EEECCCCCCCCEECCCHHHHHHHHCCCEEECEEEEEECCCCCCCHHHHHHHHHHHHCCEE
LYVSGEESSRQLKLRADRLSHDNPNCFILCETHLEQIFTQAANIQPDLMIIDSIQTIFTE
EEECCCCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
VVESSPGSVSQVRECSAAILKYAKESGVPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEG
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
DQHYMYRILRSIKNRFGSTAELGIYEMRQNGLREVSNPSELLLTQNHEGLSGVAIAAAIE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHH
GVRPFLIETQALVSSAVYGTPQRSATGFDLRRMNMLLAVLEKRAGFKLIQKDVFLNIAGG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEEEEECC
LKVNDPAIDLAVISSILSSSLDISIEPGVCMAGEVGLSGEIRPVNRIEQRILEAEKLGFS
CEECCCHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCE
RIIIPHNNLKGFDTAKSKIQIVQVKKVEEAFRQLFG
EEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]