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The map label for this gene is iaaA [H]

Identifier: 150008381

GI number: 150008381

Start: 2082626

End: 2083624

Strand: Reverse

Name: iaaA [H]

Synonym: BDI_1755

Alternate gene names: 150008381

Gene position: 2083624-2082626 (Counterclockwise)

Preceding gene: 150008382

Following gene: 150008380

Centisome position: 43.31

GC content: 50.65

Gene sequence:

>999_bases
ATGTTAAAACAAATGATAGCCTTGACGGCTTTGATGGTGTCATCCATTGCTTTTGCGCAAGACCGGGAGTATGTAATCGT
GGTACATGGTGGTGCGGGGGCGATGGCCGGATTGGAGGAAGATCCGGTTAAGTCGGCTCAATATTACGCCGCATTGGACT
CCGCTTTAATGATTGGAGATAAGGTCCTTTCTGCGGGAGGCGAGGGGCCGGAAGCGGTGATGGCCGTAATCAATTACTTC
GAGGGAAATCCGTTGTTTAACGCCGGAATTGGTGCTACCTGTACGGCGGAGGGAACTTTTGAGTTGGACGCTTCCATCAT
GGAAGGCAAGGATTTAAGCGCAGGCGCTGTAGCTGGCTTAAAACACGTGAAGCATCCGATCAATGCCGCCTATGCCGTAA
AGACAAAATCTCCGCATGTCATGCTGAGCGGAGCCGGTGCTGAGGAGTTCGCCAAGGAACAAGGCTTGGAAATGGTAGAA
GACAATATGTATTTCGCTACGCCCAAGACGATGGAGTGGATCGAGAAGTTAAAGCAAGAAAGTAAGAAGAACGGTACGGT
AGGCTGTGTCGTTCTGGATAAGCAAGGAAACTTAACGGCCGGGACCAGTACCGGCGGTATGTTCAAGAAACGTTGGGGGC
GTATCGGTGATTCGCCCGTGATCGGGGCGGGAACGTATGCGGATAATAACAGTTGCGCCGTCTCCTGTACGGGGCATGGC
GAGTATTTTATCCGTCATGCGGTAGCGTTCGATGTGTGTGCCCGCTATAAATACCTTAAGGAAAGCGTAGAGAAAGCGGC
AGATTATATCATCCATACGGAATTGAATACGAACGCCGGCAACGGCGGCTTGATCGCCGTGGATAAACTAGGAAATATCG
CCATGCCTTATAACAGTGGCGGTATGTTCCGCGGATTCTTGTATAAGGAGAAAGGTAGCCCGGAAATTAAGAAGGGCTGC
GGTATCGGAAAGAAGATCGTTGTAGTAGGGGAAAAGTAG

Upstream 100 bases:

>100_bases
AAATGTACGGATAAAAATCGGGAAATAAGTCTATGTCTCTAAGATAATTCAGATATTTGTTCTATTTTTGAACATTGTAT
AAATGATTAAATATAATAGC

Downstream 100 bases:

>100_bases
TTTTTTGTATCTTTACGCCAAATTCAAGATAGAATGGCGAAAACAAAAACCGTATATGTCTGCTCTAACTGTGGCGCTGA
TTCCCCGAAATGGCTGGGTA

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKQMIALTALMVSSIAFAQDREYVIVVHGGAGAMAGLEEDPVKSAQYYAALDSALMIGDKVLSAGGEGPEAVMAVINYF
EGNPLFNAGIGATCTAEGTFELDASIMEGKDLSAGAVAGLKHVKHPINAAYAVKTKSPHVMLSGAGAEEFAKEQGLEMVE
DNMYFATPKTMEWIEKLKQESKKNGTVGCVVLDKQGNLTAGTSTGGMFKKRWGRIGDSPVIGAGTYADNNSCAVSCTGHG
EYFIRHAVAFDVCARYKYLKESVEKAADYIIHTELNTNAGNGGLIAVDKLGNIAMPYNSGGMFRGFLYKEKGSPEIKKGC
GIGKKIVVVGEK

Sequences:

>Translated_332_residues
MLKQMIALTALMVSSIAFAQDREYVIVVHGGAGAMAGLEEDPVKSAQYYAALDSALMIGDKVLSAGGEGPEAVMAVINYF
EGNPLFNAGIGATCTAEGTFELDASIMEGKDLSAGAVAGLKHVKHPINAAYAVKTKSPHVMLSGAGAEEFAKEQGLEMVE
DNMYFATPKTMEWIEKLKQESKKNGTVGCVVLDKQGNLTAGTSTGGMFKKRWGRIGDSPVIGAGTYADNNSCAVSCTGHG
EYFIRHAVAFDVCARYKYLKESVEKAADYIIHTELNTNAGNGGLIAVDKLGNIAMPYNSGGMFRGFLYKEKGSPEIKKGC
GIGKKIVVVGEK
>Mature_332_residues
MLKQMIALTALMVSSIAFAQDREYVIVVHGGAGAMAGLEEDPVKSAQYYAALDSALMIGDKVLSAGGEGPEAVMAVINYF
EGNPLFNAGIGATCTAEGTFELDASIMEGKDLSAGAVAGLKHVKHPINAAYAVKTKSPHVMLSGAGAEEFAKEQGLEMVE
DNMYFATPKTMEWIEKLKQESKKNGTVGCVVLDKQGNLTAGTSTGGMFKKRWGRIGDSPVIGAGTYADNNSCAVSCTGHG
EYFIRHAVAFDVCARYKYLKESVEKAADYIIHTELNTNAGNGGLIAVDKLGNIAMPYNSGGMFRGFLYKEKGSPEIKKGC
GIGKKIVVVGEK

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=287, Percent_Identity=38.6759581881533, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI145275200, Length=287, Percent_Identity=38.6759581881533, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI98991769, Length=277, Percent_Identity=28.8808664259928, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI285002251, Length=271, Percent_Identity=25.830258302583, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1787050, Length=311, Percent_Identity=42.443729903537, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32564649, Length=252, Percent_Identity=26.1904761904762, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI18921183, Length=312, Percent_Identity=33.974358974359, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=27.6119402985075, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24652135, Length=247, Percent_Identity=29.5546558704453, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221330102, Length=247, Percent_Identity=29.5546558704453, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24655553, Length=267, Percent_Identity=25.0936329588015, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 35149; Mature: 35149

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQMIALTALMVSSIAFAQDREYVIVVHGGAGAMAGLEEDPVKSAQYYAALDSALMIGD
CHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KVLSAGGEGPEAVMAVINYFEGNPLFNAGIGATCTAEGTFELDASIMEGKDLSAGAVAGL
HHHHCCCCCHHHHHHHHHHHCCCCEEECCCCEEEECCCEEEECCCEECCCCCCHHHHHHH
KHVKHPINAAYAVKTKSPHVMLSGAGAEEFAKEQGLEMVEDNMYFATPKTMEWIEKLKQE
HHHCCCCCCEEEEECCCCEEEEECCCHHHHHHHCCCHHHHCCEEEECCHHHHHHHHHHHH
SKKNGTVGCVVLDKQGNLTAGTSTGGMFKKRWGRIGDSPVIGAGTYADNNSCAVSCTGHG
HCCCCCEEEEEEECCCCEECCCCCCCHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCC
EYFIRHAVAFDVCARYKYLKESVEKAADYIIHTELNTNAGNGGLIAVDKLGNIAMPYNSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEECCCCEEEEECCC
GMFRGFLYKEKGSPEIKKGCGIGKKIVVVGEK
CEEEEEEEECCCCCHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MLKQMIALTALMVSSIAFAQDREYVIVVHGGAGAMAGLEEDPVKSAQYYAALDSALMIGD
CHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KVLSAGGEGPEAVMAVINYFEGNPLFNAGIGATCTAEGTFELDASIMEGKDLSAGAVAGL
HHHHCCCCCHHHHHHHHHHHCCCCEEECCCCEEEECCCEEEECCCEECCCCCCHHHHHHH
KHVKHPINAAYAVKTKSPHVMLSGAGAEEFAKEQGLEMVEDNMYFATPKTMEWIEKLKQE
HHHCCCCCCEEEEECCCCEEEEECCCHHHHHHHCCCHHHHCCEEEECCHHHHHHHHHHHH
SKKNGTVGCVVLDKQGNLTAGTSTGGMFKKRWGRIGDSPVIGAGTYADNNSCAVSCTGHG
HCCCCCEEEEEEECCCCEECCCCCCCHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCC
EYFIRHAVAFDVCARYKYLKESVEKAADYIIHTELNTNAGNGGLIAVDKLGNIAMPYNSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEECCCCEEEEECCC
GMFRGFLYKEKGSPEIKKGCGIGKKIVVVGEK
CEEEEEEEECCCCCHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]