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The map label for this gene is oprM [H]

Identifier: 150008362

GI number: 150008362

Start: 2055548

End: 2056954

Strand: Direct

Name: oprM [H]

Synonym: BDI_1736

Alternate gene names: 150008362

Gene position: 2055548-2056954 (Clockwise)

Preceding gene: 150008359

Following gene: 150008363

Centisome position: 42.72

GC content: 53.02

Gene sequence:

>1407_bases
ATGCACAAACATTTACGCCTGACATGTTATGCTTTGTTGTGCTTGCTGGTACCGATCGGGGTAATGGCGCAGACAGAGCA
TACGTATTTAGACCGTGCCCTACCCGGATCATGGTCTGAGGAAGGGAGTGATTTCCAGCAGACACTTCCCGTAGAAGATA
ATTGGTGGAGGAATTTTAAGGACCCGTTACTGGACTCGCTGATAGAAGTAGCCGTGAAACAAAACTATTCCGTGCAAATG
GCGATGGACAGGATCGCCATGGCGAAAGCCAACCTGCGGTCGGCACAAGGAAGCTACTCGCCCACCCTAGGGCTTTCCGC
CGGATGGACACGGCAACAAAGTAGCGGCAATATCAATCAGGTACCCAAGGCGATCACCCAATATTCCAGCGCTACCGTAG
ATATGAACTGGGAAGTAGACGTATTCGGAAGTATCCGGAACCGGGTGAAAGCGGAGAAAGAGAATTTCGCCGCCTCGAAA
GAGGAATACAACGCCGCCATGGTCTCCCTTTGCGCCCAAGTAGCCTCCTCCTATATCAATATGAGGGAACTGCAACAAGA
GGTAAAGGTAGCGCAACAGAACTGCCGCTCCCAACAGACGGTCGTACAGATCACCCAGAAACGGTATGAGACCGGGCTAG
TCTCTAAACTGGACGTAGCGCAGGCGCAATCCGTATACTACAGCACGAAAGCCTCCCTCCCGATGCTGGAGGCCGGTATC
ATCCAATATACGAACTCCTTGGCGATCTTGCTAGGCCTGTACCCTTCCGATCTACAAAAGATCATGGAGACAGACGCTTC
CTTGCCGGAATATATCGAGCCGGTCGGTGTAGGGCTACCGGCAAACCTGTTGCTACGCCGTCCGGATGTACGTGCGGCAG
AACGGCAGGTCAATGCGCTTGCCGCCTCGCTCGGGGCCTCTAAGTCCGATTGGTGGCCGAAAGTATTCGTGAAAGGCTCG
ATCGGTTTCGCCTCCCACGACTTCGACAAGCTGGCGAATCATAATAGCTTGACCTATGAGATCGCCCCTACCCTTACGTG
GAACTTCTTCCAAGGCACCCAGAAGATACAGGCTACCCGGTTGGCGAAAGCCCAGTTGGACGAGTCGATCCGGCAATTCA
ACCAGACCGTGCTTACCTCCGTACAGGAAGTGGATAACGCCATGAGCTCGTACAAGAACTCGATCAAGCAGATCGTAGCC
CTCCGGGAAGTGGTAAACCAAGGGAAACAAACCTTGGACCTTTCCCTAGACCTCTATAAGCAGGGCCTTACGCCTTTCCA
AAACGTACTGGACGCGCAACGCTCCCTCCTCACCTACCAGAACCAATTGACACAGGCTCAAGGTAGCTCCCTACTGAACT
TGATACAGCTTTATCAGTCATTGGGCGGAGGATGGAATCCTATGTAG

Upstream 100 bases:

>100_bases
TCCATATATTTCAGTTTTTGGGATATATAAATAGACAAAGATACAAACATTTCCCACCCTTAACTGTTTTGCTAAAAGCT
AAATCTAAACATATATATTT

Downstream 100 bases:

>100_bases
GCCGTTCGATCCGATCGAACAAAAGCCCACTCAAACAATTAATATAAATAATCATTAAAGACAAAAGATATGAAGACATT
TGTTTACATAGGGATTATCG

Product: outer membrane efflux protein oprM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MHKHLRLTCYALLCLLVPIGVMAQTEHTYLDRALPGSWSEEGSDFQQTLPVEDNWWRNFKDPLLDSLIEVAVKQNYSVQM
AMDRIAMAKANLRSAQGSYSPTLGLSAGWTRQQSSGNINQVPKAITQYSSATVDMNWEVDVFGSIRNRVKAEKENFAASK
EEYNAAMVSLCAQVASSYINMRELQQEVKVAQQNCRSQQTVVQITQKRYETGLVSKLDVAQAQSVYYSTKASLPMLEAGI
IQYTNSLAILLGLYPSDLQKIMETDASLPEYIEPVGVGLPANLLLRRPDVRAAERQVNALAASLGASKSDWWPKVFVKGS
IGFASHDFDKLANHNSLTYEIAPTLTWNFFQGTQKIQATRLAKAQLDESIRQFNQTVLTSVQEVDNAMSSYKNSIKQIVA
LREVVNQGKQTLDLSLDLYKQGLTPFQNVLDAQRSLLTYQNQLTQAQGSSLLNLIQLYQSLGGGWNPM

Sequences:

>Translated_468_residues
MHKHLRLTCYALLCLLVPIGVMAQTEHTYLDRALPGSWSEEGSDFQQTLPVEDNWWRNFKDPLLDSLIEVAVKQNYSVQM
AMDRIAMAKANLRSAQGSYSPTLGLSAGWTRQQSSGNINQVPKAITQYSSATVDMNWEVDVFGSIRNRVKAEKENFAASK
EEYNAAMVSLCAQVASSYINMRELQQEVKVAQQNCRSQQTVVQITQKRYETGLVSKLDVAQAQSVYYSTKASLPMLEAGI
IQYTNSLAILLGLYPSDLQKIMETDASLPEYIEPVGVGLPANLLLRRPDVRAAERQVNALAASLGASKSDWWPKVFVKGS
IGFASHDFDKLANHNSLTYEIAPTLTWNFFQGTQKIQATRLAKAQLDESIRQFNQTVLTSVQEVDNAMSSYKNSIKQIVA
LREVVNQGKQTLDLSLDLYKQGLTPFQNVLDAQRSLLTYQNQLTQAQGSSLLNLIQLYQSLGGGWNPM
>Mature_468_residues
MHKHLRLTCYALLCLLVPIGVMAQTEHTYLDRALPGSWSEEGSDFQQTLPVEDNWWRNFKDPLLDSLIEVAVKQNYSVQM
AMDRIAMAKANLRSAQGSYSPTLGLSAGWTRQQSSGNINQVPKAITQYSSATVDMNWEVDVFGSIRNRVKAEKENFAASK
EEYNAAMVSLCAQVASSYINMRELQQEVKVAQQNCRSQQTVVQITQKRYETGLVSKLDVAQAQSVYYSTKASLPMLEAGI
IQYTNSLAILLGLYPSDLQKIMETDASLPEYIEPVGVGLPANLLLRRPDVRAAERQVNALAASLGASKSDWWPKVFVKGS
IGFASHDFDKLANHNSLTYEIAPTLTWNFFQGTQKIQATRLAKAQLDESIRQFNQTVLTSVQEVDNAMSSYKNSIKQIVA
LREVVNQGKQTLDLSLDLYKQGLTPFQNVLDAQRSLLTYQNQLTQAQGSSLLNLIQLYQSLGGGWNPM

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=419, Percent_Identity=28.1622911694511, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI2367348, Length=433, Percent_Identity=25.4041570438799, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52210; Mature: 52210

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]