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The map label for this gene is hyi [C]

Identifier: 150008272

GI number: 150008272

Start: 1938994

End: 1940004

Strand: Reverse

Name: hyi [C]

Synonym: BDI_1641

Alternate gene names: 150008272

Gene position: 1940004-1938994 (Counterclockwise)

Preceding gene: 150008275

Following gene: 150008271

Centisome position: 40.32

GC content: 47.58

Gene sequence:

>1011_bases
ATGATGTGTTTTTCATGTAGAGTGACTTCCGATGCAAAAGTAGTGTTTTTGGATGTAATACCAACGCCTCAGGATATTAT
ATCCGATTTTTCTTATATTTGTCTCCTATTAACTATCAAATTCGAATACATGGAAAACATATCAAGAAGAACGGCTATCA
AGACATTTTTAGCTGGAGGCGTGGCTTTGGCGACTACAGGTATCGAGGCGGCAGCCGCCGCGAAAAAGAAAACAGACGTG
AAGGAACCCTTGAAAGGAAATGTCCGCCACTCCGTAAGTAAATGGTGTTTCGGCGATTATAAGCTGGATGAGTTCTGTGA
GATCTGCAAGCATATAGGGATTGAATCCGTTGAGTTGCTGGACCCGGTAGATTGGCCGATCGTACAAAAACATGGCCTGA
CGGTGGCGATGGCGCAAGGGGCCGGGCTTGGAATAGATCGAGGTTTCAATGATCCGGCCTTGCATGACGAGTTGGTCGCC
AGTTATGAGAAGGTGATCCCGATGGTGGCGGACGCTGGATTGACAAACTTGATTTGCTTCTCCGGCAGACGTAATGGCGT
AACGGACTTGCAAGGCTGGGAGAACTGCGAGAAGGGGTTGAAGCGCCTTATCCCGTTAGCGGAGAAACACAAGGTCGTCT
TGACCATGGAGCTTTTGAACAGTGTCGGTCATAAGGATTATCTGTGCGATCATACGGTTTGGGGTGCGGAGCTTTGTCGC
CGTATCGGTTCCCCTAATTTTAAGTTACTATATGATATTTATCATATGCAGATCATGGAGGGGAATATCATCGAGAATAT
CCGGAAATACCATCCTTATTTCTCGCACGTACATACCGGTGGAAGTCCGGGCCGTGCCGAGATCGACGAGACGCAGGAAC
TTTATTATCCGGCTATCATCAAGGCTCTTATGGAGACAGGCTATAAAGGCTTCGTTGGGCAGGAGTTCGTCCCCGCCCAA
GAAGACAAGATCGCTTCCTTGGAGAAGTGTATCCGTATCTGTGATGTATAG

Upstream 100 bases:

>100_bases
AGGCTCGCCTTGAAATACACACCTGCGCCTTCCATTCCGTATAGGGATGAAGTGCATCCTATTATAAAGAATAGGATCAG
ATAAATAGATCGTTTTGTAT

Downstream 100 bases:

>100_bases
GGCTATTTAGTAATAACTTACAGTGCCAAGCCAGTGCCGTTATAGTGGCACGGCTGTGCCACTAGCGTGGCATCGTTGTG
CCATCGCTATGGCATCATCG

Product: hypothetical protein

Products: 2-Hydroxy-3-oxopropanoate [C]

Alternate protein names: Xylose Isomerase Domain Protein TIM Barrel; Xylose Isomerase Domain-Containing Protein; Glyoxylate-Induced Protein; Hydroxypyruvate Isomerase Protein; Twin-Arginine Translocation Pathway Signal; AP Endonuclease; Xylose Isomerase-Like TIM Barrel; Sugar Isomerase; AP Endonuclease Family 2 Superfamily; Xylose Isomerase DomaiN-Containing Protein Tim Barrel; Xylose Isomerase Domain-Containing Protein TIM Barrel; Epimerase Protein; Xylose Isomerase; Sugar Phosphate Isomerase/Epimerase; Regulatory Protein GclR; Xylose Isomerase-Like Protein; 2 Superfamily AP Endonuclease; AP Endonuclease Family Superfamily Protein

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MMCFSCRVTSDAKVVFLDVIPTPQDIISDFSYICLLLTIKFEYMENISRRTAIKTFLAGGVALATTGIEAAAAAKKKTDV
KEPLKGNVRHSVSKWCFGDYKLDEFCEICKHIGIESVELLDPVDWPIVQKHGLTVAMAQGAGLGIDRGFNDPALHDELVA
SYEKVIPMVADAGLTNLICFSGRRNGVTDLQGWENCEKGLKRLIPLAEKHKVVLTMELLNSVGHKDYLCDHTVWGAELCR
RIGSPNFKLLYDIYHMQIMEGNIIENIRKYHPYFSHVHTGGSPGRAEIDETQELYYPAIIKALMETGYKGFVGQEFVPAQ
EDKIASLEKCIRICDV

Sequences:

>Translated_336_residues
MMCFSCRVTSDAKVVFLDVIPTPQDIISDFSYICLLLTIKFEYMENISRRTAIKTFLAGGVALATTGIEAAAAAKKKTDV
KEPLKGNVRHSVSKWCFGDYKLDEFCEICKHIGIESVELLDPVDWPIVQKHGLTVAMAQGAGLGIDRGFNDPALHDELVA
SYEKVIPMVADAGLTNLICFSGRRNGVTDLQGWENCEKGLKRLIPLAEKHKVVLTMELLNSVGHKDYLCDHTVWGAELCR
RIGSPNFKLLYDIYHMQIMEGNIIENIRKYHPYFSHVHTGGSPGRAEIDETQELYYPAIIKALMETGYKGFVGQEFVPAQ
EDKIASLEKCIRICDV
>Mature_336_residues
MMCFSCRVTSDAKVVFLDVIPTPQDIISDFSYICLLLTIKFEYMENISRRTAIKTFLAGGVALATTGIEAAAAAKKKTDV
KEPLKGNVRHSVSKWCFGDYKLDEFCEICKHIGIESVELLDPVDWPIVQKHGLTVAMAQGAGLGIDRGFNDPALHDELVA
SYEKVIPMVADAGLTNLICFSGRRNGVTDLQGWENCEKGLKRLIPLAEKHKVVLTMELLNSVGHKDYLCDHTVWGAELCR
RIGSPNFKLLYDIYHMQIMEGNIIENIRKYHPYFSHVHTGGSPGRAEIDETQELYYPAIIKALMETGYKGFVGQEFVPAQ
EDKIASLEKCIRICDV

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI300068962, Length=113, Percent_Identity=37.1681415929204, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI300068964, Length=108, Percent_Identity=37.037037037037, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786718, Length=233, Percent_Identity=24.8927038626609, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.3.1.22 [C]

Molecular weight: Translated: 37553; Mature: 37553

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMCFSCRVTSDAKVVFLDVIPTPQDIISDFSYICLLLTIKFEYMENISRRTAIKTFLAGG
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VALATTGIEAAAAAKKKTDVKEPLKGNVRHSVSKWCFGDYKLDEFCEICKHIGIESVELL
HHEECCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHC
DPVDWPIVQKHGLTVAMAQGAGLGIDRGFNDPALHDELVASYEKVIPMVADAGLTNLICF
CCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEE
SGRRNGVTDLQGWENCEKGLKRLIPLAEKHKVVLTMELLNSVGHKDYLCDHTVWGAELCR
CCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHH
RIGSPNFKLLYDIYHMQIMEGNIIENIRKYHPYFSHVHTGGSPGRAEIDETQELYYPAII
HHCCCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KALMETGYKGFVGQEFVPAQEDKIASLEKCIRICDV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMCFSCRVTSDAKVVFLDVIPTPQDIISDFSYICLLLTIKFEYMENISRRTAIKTFLAGG
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VALATTGIEAAAAAKKKTDVKEPLKGNVRHSVSKWCFGDYKLDEFCEICKHIGIESVELL
HHEECCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHC
DPVDWPIVQKHGLTVAMAQGAGLGIDRGFNDPALHDELVASYEKVIPMVADAGLTNLICF
CCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEE
SGRRNGVTDLQGWENCEKGLKRLIPLAEKHKVVLTMELLNSVGHKDYLCDHTVWGAELCR
CCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHH
RIGSPNFKLLYDIYHMQIMEGNIIENIRKYHPYFSHVHTGGSPGRAEIDETQELYYPAII
HHCCCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KALMETGYKGFVGQEFVPAQEDKIASLEKCIRICDV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: Hydroxypyruvate [C]

Specific reaction: Hydroxypyruvate <==> 2-Hydroxy-3-oxopropanoate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA