Click here to switch to the map view.

The map label for this gene is rfbX [H]

Identifier: 150008102

GI number: 150008102

Start: 1708374

End: 1709618

Strand: Direct

Name: rfbX [H]

Synonym: BDI_1466

Alternate gene names: 150008102

Gene position: 1708374-1709618 (Clockwise)

Preceding gene: 150008101

Following gene: 150008104

Centisome position: 35.51

GC content: 38.71

Gene sequence:

>1245_bases
ATGGAAATAAAAAAGAAGCTCAAGAATCCTATGGTTATAAATTTTATCTCCTTGGCATTGCTCCAAGGAGTTAATTATTT
ATTACCTTTATTATCATTCCCTTTTCTTTTCAGAGTACTCGGTGTAGAACGCTGGGGGCTCGTGACGTTCGGCTATGCCT
TGATGCAATATTTCATTATGTTTACGGATTTTGGCTTTAACCTATCAGCCACGAAATATATCTCTGAGCATAAAACGGAT
TTAGGTAAAATCAACTCTTACCTAAATAGCACAATGCTTGGACGTTTCTTTTTATGTGGGGTCAGCTTCGCCATACTACT
CATTCTGATCACTTGTTTTGATAAGTTTAAGTCAGAATCTTCTTTTTATCTCCTATATTTTGGCATGATCATAGGCAATG
TTATGTTTCCTATGTGGTTTTTTCAAGGCATGGAGAATATGAAATACATTACGGTATTCAATATCGTAGCGAAATCATTG
AGTTTCCTTCCCTTTTTCATCTTCATTCGAAAACCGGAAAATTATATATACGTACCGATATGCTATAGTTTTGGCTTTAT
TCTTGCCGGGATCGTTAGTATCTTTATTGTTTACTTCAGAATGGGGATGAAATGGTACTTCACATCAATCGAAGAAGTTA
GTAACTCCTTAAAAGATAGTTCCACTTATTTTCTCTCCCGGGCCTCTACCTCTTTGTTCACCACAAGCAACTCGTTTCTA
TTGGGTTTGATTTGCGGAAACACAGCGGTGGGTTACTATTCGGCAGCAGAGAAGCTATATCAAGCGTACAATCAGTTATT
GACACCATTCACTGGGGTTTTATTTCCGCATATAGCGAAAAGTAGAGATGTCTTGTTCTTTAAAAAAGTATTCTATAGAA
TAACATTCTCCAACTTCTTTTGCGTAACGACCGTACTTTTATTCTCAAGTTATGTATTAAGATTTGTCTATGGGACAGCC
GATCCGGATATCTTAGAAGTATTCCGGATACTTGTAATCGCTTGTTTCGTAACCATCCCCTCTATGTTACTAGGATATCC
ATTTCTGGCGGCCATGGGGCACCCTCTATATACGAACTATACAGTTATGACCACCTCCATGGTACACATCACTGGATTAG
CGATCCTATATGTTTGCGGAATGATCGCCCCTATCACGGTAGCAATGATGGTAGTCGTGTCTGAGACACTTCTTTTCCTT
TTCCGTATTTGGGGGATACGTAGGTTCGGGCTATTCCGAGCGTAA

Upstream 100 bases:

>100_bases
TATATCCGAATCGCTATCCGGAACAAGGAAGACAATGATAAGCTAATAGAAGCGCTGACTTCATTGAATAAATAAGGAAG
TTCAATCCGGTCTTTATTAT

Downstream 100 bases:

>100_bases
GAAGCTGAGGCAAATTTGACAAGTGATTTAAGAAAAGACATATCTTAGCTTCATCAACCAAGTATCTTAGGTTCGTTGGC
AAAGTATGTTAAGTTACTTT

Product: O-antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEIKKKLKNPMVINFISLALLQGVNYLLPLLSFPFLFRVLGVERWGLVTFGYALMQYFIMFTDFGFNLSATKYISEHKTD
LGKINSYLNSTMLGRFFLCGVSFAILLILITCFDKFKSESSFYLLYFGMIIGNVMFPMWFFQGMENMKYITVFNIVAKSL
SFLPFFIFIRKPENYIYVPICYSFGFILAGIVSIFIVYFRMGMKWYFTSIEEVSNSLKDSSTYFLSRASTSLFTTSNSFL
LGLICGNTAVGYYSAAEKLYQAYNQLLTPFTGVLFPHIAKSRDVLFFKKVFYRITFSNFFCVTTVLLFSSYVLRFVYGTA
DPDILEVFRILVIACFVTIPSMLLGYPFLAAMGHPLYTNYTVMTTSMVHITGLAILYVCGMIAPITVAMMVVVSETLLFL
FRIWGIRRFGLFRA

Sequences:

>Translated_414_residues
MEIKKKLKNPMVINFISLALLQGVNYLLPLLSFPFLFRVLGVERWGLVTFGYALMQYFIMFTDFGFNLSATKYISEHKTD
LGKINSYLNSTMLGRFFLCGVSFAILLILITCFDKFKSESSFYLLYFGMIIGNVMFPMWFFQGMENMKYITVFNIVAKSL
SFLPFFIFIRKPENYIYVPICYSFGFILAGIVSIFIVYFRMGMKWYFTSIEEVSNSLKDSSTYFLSRASTSLFTTSNSFL
LGLICGNTAVGYYSAAEKLYQAYNQLLTPFTGVLFPHIAKSRDVLFFKKVFYRITFSNFFCVTTVLLFSSYVLRFVYGTA
DPDILEVFRILVIACFVTIPSMLLGYPFLAAMGHPLYTNYTVMTTSMVHITGLAILYVCGMIAPITVAMMVVVSETLLFL
FRIWGIRRFGLFRA
>Mature_414_residues
MEIKKKLKNPMVINFISLALLQGVNYLLPLLSFPFLFRVLGVERWGLVTFGYALMQYFIMFTDFGFNLSATKYISEHKTD
LGKINSYLNSTMLGRFFLCGVSFAILLILITCFDKFKSESSFYLLYFGMIIGNVMFPMWFFQGMENMKYITVFNIVAKSL
SFLPFFIFIRKPENYIYVPICYSFGFILAGIVSIFIVYFRMGMKWYFTSIEEVSNSLKDSSTYFLSRASTSLFTTSNSFL
LGLICGNTAVGYYSAAEKLYQAYNQLLTPFTGVLFPHIAKSRDVLFFKKVFYRITFSNFFCVTTVLLFSSYVLRFVYGTA
DPDILEVFRILVIACFVTIPSMLLGYPFLAAMGHPLYTNYTVMTTSMVHITGLAILYVCGMIAPITVAMMVVVSETLLFL
FRIWGIRRFGLFRA

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=283, Percent_Identity=32.1554770318021, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 47532; Mature: 47532

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKKKLKNPMVINFISLALLQGVNYLLPLLSFPFLFRVLGVERWGLVTFGYALMQYFIM
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FTDFGFNLSATKYISEHKTDLGKINSYLNSTMLGRFFLCGVSFAILLILITCFDKFKSES
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SFYLLYFGMIIGNVMFPMWFFQGMENMKYITVFNIVAKSLSFLPFFIFIRKPENYIYVPI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEH
CYSFGFILAGIVSIFIVYFRMGMKWYFTSIEEVSNSLKDSSTYFLSRASTSLFTTSNSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCEE
LGLICGNTAVGYYSAAEKLYQAYNQLLTPFTGVLFPHIAKSRDVLFFKKVFYRITFSNFF
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
CVTTVLLFSSYVLRFVYGTADPDILEVFRILVIACFVTIPSMLLGYPFLAAMGHPLYTNY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCE
TVMTTSMVHITGLAILYVCGMIAPITVAMMVVVSETLLFLFRIWGIRRFGLFRA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEIKKKLKNPMVINFISLALLQGVNYLLPLLSFPFLFRVLGVERWGLVTFGYALMQYFIM
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FTDFGFNLSATKYISEHKTDLGKINSYLNSTMLGRFFLCGVSFAILLILITCFDKFKSES
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SFYLLYFGMIIGNVMFPMWFFQGMENMKYITVFNIVAKSLSFLPFFIFIRKPENYIYVPI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEH
CYSFGFILAGIVSIFIVYFRMGMKWYFTSIEEVSNSLKDSSTYFLSRASTSLFTTSNSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCEE
LGLICGNTAVGYYSAAEKLYQAYNQLLTPFTGVLFPHIAKSRDVLFFKKVFYRITFSNFF
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
CVTTVLLFSSYVLRFVYGTADPDILEVFRILVIACFVTIPSMLLGYPFLAAMGHPLYTNY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCE
TVMTTSMVHITGLAILYVCGMIAPITVAMMVVVSETLLFLFRIWGIRRFGLFRA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]