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The map label for this gene is glnS [H]

Identifier: 150008025

GI number: 150008025

Start: 1610666

End: 1612405

Strand: Direct

Name: glnS [H]

Synonym: BDI_1388

Alternate gene names: 150008025

Gene position: 1610666-1612405 (Clockwise)

Preceding gene: 150008024

Following gene: 150008026

Centisome position: 33.48

GC content: 51.61

Gene sequence:

>1740_bases
ATGAGCGATATCAATACAAACGAAGAAGAAGGCAAGAAGAACCTGAATTTCATTGAGGCAGCCGTAGAGAAAGATTTAGC
GGAAGGCAAGAACGGAGGACGTGTACAAACCCGTTTCCCGCCCGAGCCCAACGGATACCTTCACATCGGCCACGCGAAGG
CCATTTGCCTAGATTTCGGCATCGCTGAACGCCATGGCGGTATCTGCAACCTCCGTTTCGACGACACGAACCCGACGAAG
GAGGATGAGGAATACGTGGAGGCGATCAAGGAGGATATCCAATGGCTGGGATATCAGTGGGGAAATATTTATTACGCGTC
CGACTACTTCCAGCAATTGTGGGATTTCGCGATCCGCCTCATCGAGGAAGGTAAAGCATATATTGACGAGCAGACATCCG
AACAAATAGCCCAGCAAAAGGGCACGCCTACACAACCGGGCATCGAGAGCCCGTATCGCAACCGTCCGATCGAGGAAAGC
CTAGCTCTCTTCAAGAAGATGAACACGGGTGAGATAGCGGAAGGTGCCATGGTACTTCGCGCGAAGATAGACATGGCAAA
CCCGAACATGCACTTCCGCGATCCGATCATCTATCGGGTGGTTAACCATCCGCACCATCGTACGGGTACGACGTGGAAAG
CGTATCCGATGTACGACTTCGCCCACGGACAGAGCGATTTCTTCGAGGGCGTGACGCATTCCTTGTGTACCTTGGAGTTT
GTCGTACACCGTCCTCTGTATGATTTGTTTATCGATTGGCTAAAGGAAGGCGAGGACTTGAACGACAACCGCCCCCGACA
GACTGAGTTCAACAAATTGAACCTAAGCTACACGCTGATGAGCAAGCGTAACCTGCTTACATTGGTAAAAGAGAACTTGG
TAAACGGCTGGGATGATCCCCGTATGCCGACCATCTGCGGTTTCCGCCGCCGGGGTTACTCTCCGGAGTCTATCCGTAAG
TTCATCGACAAGATCGGTTATACGACTTACGACGCCCTGAACGAGTTCGCCTTGCTGGAAAGCGCCGTACGCGAGGATCT
GAATGCCCGCGCCATCCGTGTATCCGCCGTGATCAATCCGGTGAAACTGATTATCACCAACTATCCGGAAGGACAGGTGG
AGGAGCTGGAAGCGATCAACAACCCGGAAGATCCGGAAGCCGGCAGCCATACGATCGAGTTCAGCCGTGAGTTGTGGATG
GAACGTGAGGATTTCATGGAAGACGCTCCCAAGAAATATTTCCGGATGACACCGGGACAGGAAGTTCGTCTGAAGAACGC
CTATATCGTAAAATGTACGGGCTGCAAGAAAGACGAGAACGGTGAGATTACCGAGGTATATTGCGAATACGATCCGAATA
CCCGTAGCGGTATGCCGGACGCTAACCGTAAAGTAAAAGGTACCTTGCACTGGGTAAGCTGCGCCCATTGCCAAAAAGCG
GAAGTACGCTTATACGACCGCCTTTGGAAGGTGGAGAACCCCAGAGACGAATTGGCCGCTATCCGGGAGGAGAAGAATTG
CTCCCCGCTAGAGGCCATGAAGGAAATCATCAACCCGAACTCCTTGGAGATCCGGAAAAACTGTTACGTAGAGAAGTTCG
TGGCTACCTTGCCCAAGCTTTCCTACCTTCAATTCCAGCGTATCGGCTATTTCAACATAGATAGCGAATCCACCCCCGAC
AACTTGGTATTCAACCGTACCGTAGGGCTAAAAGACACGTGGAGCAAGATTAATAAATAA

Upstream 100 bases:

>100_bases
ACGATGGTAACCGTCCTAAATATAGCCCTGCTCATCAGCCTATTTTAAAAAGAAATCGCTACCTTTGCCCCATTCGAAGA
AAAACTATCAATTTTTAAGT

Downstream 100 bases:

>100_bases
AGAATAGTAAATGAAAAAAGACGATATCTCACGCTTGTTACAACATTATCTTTCAGCGAAGGAAGAGGGTAAGGAGCCTT
ATTTCGACGCTGATCAAATC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MSDINTNEEEGKKNLNFIEAAVEKDLAEGKNGGRVQTRFPPEPNGYLHIGHAKAICLDFGIAERHGGICNLRFDDTNPTK
EDEEYVEAIKEDIQWLGYQWGNIYYASDYFQQLWDFAIRLIEEGKAYIDEQTSEQIAQQKGTPTQPGIESPYRNRPIEES
LALFKKMNTGEIAEGAMVLRAKIDMANPNMHFRDPIIYRVVNHPHHRTGTTWKAYPMYDFAHGQSDFFEGVTHSLCTLEF
VVHRPLYDLFIDWLKEGEDLNDNRPRQTEFNKLNLSYTLMSKRNLLTLVKENLVNGWDDPRMPTICGFRRRGYSPESIRK
FIDKIGYTTYDALNEFALLESAVREDLNARAIRVSAVINPVKLIITNYPEGQVEELEAINNPEDPEAGSHTIEFSRELWM
EREDFMEDAPKKYFRMTPGQEVRLKNAYIVKCTGCKKDENGEITEVYCEYDPNTRSGMPDANRKVKGTLHWVSCAHCQKA
EVRLYDRLWKVENPRDELAAIREEKNCSPLEAMKEIINPNSLEIRKNCYVEKFVATLPKLSYLQFQRIGYFNIDSESTPD
NLVFNRTVGLKDTWSKINK

Sequences:

>Translated_579_residues
MSDINTNEEEGKKNLNFIEAAVEKDLAEGKNGGRVQTRFPPEPNGYLHIGHAKAICLDFGIAERHGGICNLRFDDTNPTK
EDEEYVEAIKEDIQWLGYQWGNIYYASDYFQQLWDFAIRLIEEGKAYIDEQTSEQIAQQKGTPTQPGIESPYRNRPIEES
LALFKKMNTGEIAEGAMVLRAKIDMANPNMHFRDPIIYRVVNHPHHRTGTTWKAYPMYDFAHGQSDFFEGVTHSLCTLEF
VVHRPLYDLFIDWLKEGEDLNDNRPRQTEFNKLNLSYTLMSKRNLLTLVKENLVNGWDDPRMPTICGFRRRGYSPESIRK
FIDKIGYTTYDALNEFALLESAVREDLNARAIRVSAVINPVKLIITNYPEGQVEELEAINNPEDPEAGSHTIEFSRELWM
EREDFMEDAPKKYFRMTPGQEVRLKNAYIVKCTGCKKDENGEITEVYCEYDPNTRSGMPDANRKVKGTLHWVSCAHCQKA
EVRLYDRLWKVENPRDELAAIREEKNCSPLEAMKEIINPNSLEIRKNCYVEKFVATLPKLSYLQFQRIGYFNIDSESTPD
NLVFNRTVGLKDTWSKINK
>Mature_578_residues
SDINTNEEEGKKNLNFIEAAVEKDLAEGKNGGRVQTRFPPEPNGYLHIGHAKAICLDFGIAERHGGICNLRFDDTNPTKE
DEEYVEAIKEDIQWLGYQWGNIYYASDYFQQLWDFAIRLIEEGKAYIDEQTSEQIAQQKGTPTQPGIESPYRNRPIEESL
ALFKKMNTGEIAEGAMVLRAKIDMANPNMHFRDPIIYRVVNHPHHRTGTTWKAYPMYDFAHGQSDFFEGVTHSLCTLEFV
VHRPLYDLFIDWLKEGEDLNDNRPRQTEFNKLNLSYTLMSKRNLLTLVKENLVNGWDDPRMPTICGFRRRGYSPESIRKF
IDKIGYTTYDALNEFALLESAVREDLNARAIRVSAVINPVKLIITNYPEGQVEELEAINNPEDPEAGSHTIEFSRELWME
REDFMEDAPKKYFRMTPGQEVRLKNAYIVKCTGCKKDENGEITEVYCEYDPNTRSGMPDANRKVKGTLHWVSCAHCQKAE
VRLYDRLWKVENPRDELAAIREEKNCSPLEAMKEIINPNSLEIRKNCYVEKFVATLPKLSYLQFQRIGYFNIDSESTPDN
LVFNRTVGLKDTWSKINK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=553, Percent_Identity=41.4104882459313, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI62241042, Length=418, Percent_Identity=36.6028708133971, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1786895, Length=566, Percent_Identity=53.0035335689046, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788743, Length=352, Percent_Identity=24.4318181818182, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543332, Length=545, Percent_Identity=40.3669724770642, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17511007, Length=377, Percent_Identity=33.9522546419098, Blast_Score=208, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6324742, Length=571, Percent_Identity=39.2294220665499, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321192, Length=388, Percent_Identity=38.1443298969072, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI17864488, Length=557, Percent_Identity=40.0359066427289, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24649466, Length=418, Percent_Identity=34.688995215311, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 67028; Mature: 66897

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINTNEEEGKKNLNFIEAAVEKDLAEGKNGGRVQTRFPPEPNGYLHIGHAKAICLDFG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCEEEEEECC
IAERHGGICNLRFDDTNPTKEDEEYVEAIKEDIQWLGYQWGNIYYASDYFQQLWDFAIRL
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCHHCCCEEEHHHHHHHHHHHHHHH
IEEGKAYIDEQTSEQIAQQKGTPTQPGIESPYRNRPIEESLALFKKMNTGEIAEGAMVLR
HHHCHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCEEEEE
AKIDMANPNMHFRDPIIYRVVNHPHHRTGTTWKAYPMYDFAHGQSDFFEGVTHSLCTLEF
EEEECCCCCCCCCCCHHEEECCCCCCCCCCCEEECCCCHHCCCCHHHHHHHHHHHHHHHH
VVHRPLYDLFIDWLKEGEDLNDNRPRQTEFNKLNLSYTLMSKRNLLTLVKENLVNGWDDP
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCC
RMPTICGFRRRGYSPESIRKFIDKIGYTTYDALNEFALLESAVREDLNARAIRVSAVINP
CCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCC
VKLIITNYPEGQVEELEAINNPEDPEAGSHTIEFSRELWMEREDFMEDAPKKYFRMTPGQ
EEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCHHHEECCCCC
EVRLKNAYIVKCTGCKKDENGEITEVYCEYDPNTRSGMPDANRKVKGTLHWVSCAHCQKA
CEEECCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCHH
EVRLYDRLWKVENPRDELAAIREEKNCSPLEAMKEIINPNSLEIRKNCYVEKFVATLPKL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCH
SYLQFQRIGYFNIDSESTPDNLVFNRTVGLKDTWSKINK
HHHHHHHCCEEECCCCCCCCCEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
SDINTNEEEGKKNLNFIEAAVEKDLAEGKNGGRVQTRFPPEPNGYLHIGHAKAICLDFG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCEEEEEECC
IAERHGGICNLRFDDTNPTKEDEEYVEAIKEDIQWLGYQWGNIYYASDYFQQLWDFAIRL
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCHHCCCEEEHHHHHHHHHHHHHHH
IEEGKAYIDEQTSEQIAQQKGTPTQPGIESPYRNRPIEESLALFKKMNTGEIAEGAMVLR
HHHCHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCEEEEE
AKIDMANPNMHFRDPIIYRVVNHPHHRTGTTWKAYPMYDFAHGQSDFFEGVTHSLCTLEF
EEEECCCCCCCCCCCHHEEECCCCCCCCCCCEEECCCCHHCCCCHHHHHHHHHHHHHHHH
VVHRPLYDLFIDWLKEGEDLNDNRPRQTEFNKLNLSYTLMSKRNLLTLVKENLVNGWDDP
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCC
RMPTICGFRRRGYSPESIRKFIDKIGYTTYDALNEFALLESAVREDLNARAIRVSAVINP
CCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCC
VKLIITNYPEGQVEELEAINNPEDPEAGSHTIEFSRELWMEREDFMEDAPKKYFRMTPGQ
EEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCHHHEECCCCC
EVRLKNAYIVKCTGCKKDENGEITEVYCEYDPNTRSGMPDANRKVKGTLHWVSCAHCQKA
CEEECCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCHH
EVRLYDRLWKVENPRDELAAIREEKNCSPLEAMKEIINPNSLEIRKNCYVEKFVATLPKL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCH
SYLQFQRIGYFNIDSESTPDNLVFNRTVGLKDTWSKINK
HHHHHHHCCEEECCCCCCCCCEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]