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The map label for this gene is idnT [H]

Identifier: 150007970

GI number: 150007970

Start: 1548986

End: 1550305

Strand: Reverse

Name: idnT [H]

Synonym: BDI_1331

Alternate gene names: 150007970

Gene position: 1550305-1548986 (Counterclockwise)

Preceding gene: 150007971

Following gene: 150007969

Centisome position: 32.22

GC content: 49.32

Gene sequence:

>1320_bases
ATGTCATTTATCATTGTCGTACTTTGTTTGGCCTTATTGATTGTCTTGATATCCTACTTTAAGGTGGATGCTTTCATCGC
GTTTCTGATCTCCTCTATCTTGGCGGGCATCGCTTTGGGCATTCCGTTGGAGAAGCTGCCGGACTCGGTGAATAACGGTA
TCGGGAGTATCTTGGGTGGGTTGACGTTGATTATCGTATTAGGCGCTATGCTAGGCAAGTTGGTGGCCGAGAGCGGAGCG
GCACAGAAGATCGCTTCCGTGATGGTGTCGCTTTTTGGTACTAAGCATATACAATGGGGGCTGATGGTGACAGGCTTTGT
CGTGGGTATTCCGTTATTTTATGGGATCGGATTTGTGTTGTTGGTGCCATTGATCTTCTCGATCGTGTATAAATACAAGC
TTCCGGCGGTCTATATCGGGTTGCCGATGTTGGCGGCGTTGTCCGTGACGCATGGATTCCTGCCCCCGCATCCGTCTCCG
GTAGCCTTGGTGGTACAGTTCAACGCAAATATCGGCCTTACGCTGATCTATGGCTTGTTCTTGGCGATTCCAGCTATCAT
TATCGCCGGGCCGGTGTTCGGCAGTCGGTTGAAACATATAAAATCCGGTCCGGTCACGTTGTTCGAGCAGAAAGAGGATA
GCGGGAGATATAGCATGCCGGGCAAGGCGAATAGTTTTATCAGCGCTACGTTGCCTGTCTTTTTACTGATTATCGTGACG
GTTATTCCTTATTGCTTTAAGGATATGGGCGAGATGGCGAGCGGGATAGTCTCTTTTATTGGTGCTCCTTCCATTGTAAT
GCTGATCGCCTTGTTGTTCGCTACATATACGCTGGGCATCCGGCAGGGACGTAGTATCAAGTCGGTTATGGCAGTCTATG
GGGAGGCCGTGAAAGATATCTCTATGATCCTTTTGATCATCGCGGGTTCCGGTATCTTCAAGCAGATCATGGAGGATAGC
GGGGTAAGCAATGAGCTGGCGACAAGCCTAGAGCAATTGCCCGTTCATCCGTTGATCTTGGGTTGGTTGATCTCTGCCGT
GATCCGTTTGTGCGTGGGATCGGCTACGGTGGCGGCGTTGACGGCGGCGGGCGTGGTGATGCCTTTAGTTACGCAAACGG
GTGCGAACCCGAACTTGATGGTTTTATCTTTAGGAGCGGGTAGCTTGATGTTCTCGCATGTCAACGATTCCGGTTTCTGG
ATGTTCAAGGAATATTTTAACCTAAGCATAAAGGATACGATCTGCTCATGGTCTATCATGGAGACGATCGTGTCGGTCGT
AGGATTGATCGGGGTAATGATCTTAAATATGATTGTATAA

Upstream 100 bases:

>100_bases
TCTGTTAGCGGTAATTAGTTATCGTAAAAAGGGGTAGTTCGGAAATAAATGTTTTCTTTGTATCCCGTTTACGGATTATA
AACTAATACAGCGAAAAAAC

Downstream 100 bases:

>100_bases
TAAAAGAATGATTGAGCAATGAGAAAGAGATGGCTGCTTAGTATCGTTATTATGCTTTTCCCTTTCGTCGCCTCGTTTGC
GGCGGACCCGGAGGAAGGTA

Product: H+/gluconate symporter and related permease

Products: gluconate [Cytoplasm]; L-idonate [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: L-Ido transporter; 5-keto-D-gluconate transporter [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSFIIVVLCLALLIVLISYFKVDAFIAFLISSILAGIALGIPLEKLPDSVNNGIGSILGGLTLIIVLGAMLGKLVAESGA
AQKIASVMVSLFGTKHIQWGLMVTGFVVGIPLFYGIGFVLLVPLIFSIVYKYKLPAVYIGLPMLAALSVTHGFLPPHPSP
VALVVQFNANIGLTLIYGLFLAIPAIIIAGPVFGSRLKHIKSGPVTLFEQKEDSGRYSMPGKANSFISATLPVFLLIIVT
VIPYCFKDMGEMASGIVSFIGAPSIVMLIALLFATYTLGIRQGRSIKSVMAVYGEAVKDISMILLIIAGSGIFKQIMEDS
GVSNELATSLEQLPVHPLILGWLISAVIRLCVGSATVAALTAAGVVMPLVTQTGANPNLMVLSLGAGSLMFSHVNDSGFW
MFKEYFNLSIKDTICSWSIMETIVSVVGLIGVMILNMIV

Sequences:

>Translated_439_residues
MSFIIVVLCLALLIVLISYFKVDAFIAFLISSILAGIALGIPLEKLPDSVNNGIGSILGGLTLIIVLGAMLGKLVAESGA
AQKIASVMVSLFGTKHIQWGLMVTGFVVGIPLFYGIGFVLLVPLIFSIVYKYKLPAVYIGLPMLAALSVTHGFLPPHPSP
VALVVQFNANIGLTLIYGLFLAIPAIIIAGPVFGSRLKHIKSGPVTLFEQKEDSGRYSMPGKANSFISATLPVFLLIIVT
VIPYCFKDMGEMASGIVSFIGAPSIVMLIALLFATYTLGIRQGRSIKSVMAVYGEAVKDISMILLIIAGSGIFKQIMEDS
GVSNELATSLEQLPVHPLILGWLISAVIRLCVGSATVAALTAAGVVMPLVTQTGANPNLMVLSLGAGSLMFSHVNDSGFW
MFKEYFNLSIKDTICSWSIMETIVSVVGLIGVMILNMIV
>Mature_438_residues
SFIIVVLCLALLIVLISYFKVDAFIAFLISSILAGIALGIPLEKLPDSVNNGIGSILGGLTLIIVLGAMLGKLVAESGAA
QKIASVMVSLFGTKHIQWGLMVTGFVVGIPLFYGIGFVLLVPLIFSIVYKYKLPAVYIGLPMLAALSVTHGFLPPHPSPV
ALVVQFNANIGLTLIYGLFLAIPAIIIAGPVFGSRLKHIKSGPVTLFEQKEDSGRYSMPGKANSFISATLPVFLLIIVTV
IPYCFKDMGEMASGIVSFIGAPSIVMLIALLFATYTLGIRQGRSIKSVMAVYGEAVKDISMILLIIAGSGIFKQIMEDSG
VSNELATSLEQLPVHPLILGWLISAVIRLCVGSATVAALTAAGVVMPLVTQTGANPNLMVLSLGAGSLMFSHVNDSGFWM
FKEYFNLSIKDTICSWSIMETIVSVVGLIGVMILNMIV

Specific function: Transports L-idonate, D-gluconate and 5-keto-D- gluconate, from the periplasm across the inner membrane [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI1790716, Length=445, Percent_Identity=44.2696629213483, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI48994935, Length=440, Percent_Identity=46.1363636363636, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=39.4209354120267, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1790776, Length=445, Percent_Identity=34.1573033707865, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI48994939, Length=429, Percent_Identity=35.4312354312354, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788707, Length=441, Percent_Identity=31.7460317460317, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1789097, Length=445, Percent_Identity=31.0112359550562, Blast_Score=192, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46557; Mature: 46426

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIIVVLCLALLIVLISYFKVDAFIAFLISSILAGIALGIPLEKLPDSVNNGIGSILGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
LTLIIVLGAMLGKLVAESGAAQKIASVMVSLFGTKHIQWGLMVTGFVVGIPLFYGIGFVL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPLIFSIVYKYKLPAVYIGLPMLAALSVTHGFLPPHPSPVALVVQFNANIGLTLIYGLF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
LAIPAIIIAGPVFGSRLKHIKSGPVTLFEQKEDSGRYSMPGKANSFISATLPVFLLIIVT
HHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VIPYCFKDMGEMASGIVSFIGAPSIVMLIALLFATYTLGIRQGRSIKSVMAVYGEAVKDI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SMILLIIAGSGIFKQIMEDSGVSNELATSLEQLPVHPLILGWLISAVIRLCVGSATVAAL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHH
TAAGVVMPLVTQTGANPNLMVLSLGAGSLMFSHVNDSGFWMFKEYFNLSIKDTICSWSIM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHH
ETIVSVVGLIGVMILNMIV
HHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SFIIVVLCLALLIVLISYFKVDAFIAFLISSILAGIALGIPLEKLPDSVNNGIGSILGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
LTLIIVLGAMLGKLVAESGAAQKIASVMVSLFGTKHIQWGLMVTGFVVGIPLFYGIGFVL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPLIFSIVYKYKLPAVYIGLPMLAALSVTHGFLPPHPSPVALVVQFNANIGLTLIYGLF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
LAIPAIIIAGPVFGSRLKHIKSGPVTLFEQKEDSGRYSMPGKANSFISATLPVFLLIIVT
HHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VIPYCFKDMGEMASGIVSFIGAPSIVMLIALLFATYTLGIRQGRSIKSVMAVYGEAVKDI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SMILLIIAGSGIFKQIMEDSGVSNELATSLEQLPVHPLILGWLISAVIRLCVGSATVAAL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHH
TAAGVVMPLVTQTGANPNLMVLSLGAGSLMFSHVNDSGFWMFKEYFNLSIKDTICSWSIM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHH
ETIVSVVGLIGVMILNMIV
HHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gluconate [Periplasm]; Proton [Periplasm]; L-idonate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] Proton [Periplasm] + L-idonate [Periplasm] = L-idonate [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 9658018 [H]