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The map label for this gene is hupB2 [H]

Identifier: 150007792

GI number: 150007792

Start: 1330039

End: 1330323

Strand: Direct

Name: hupB2 [H]

Synonym: BDI_1150

Alternate gene names: 150007792

Gene position: 1330039-1330323 (Clockwise)

Preceding gene: 150007791

Following gene: 150007793

Centisome position: 27.64

GC content: 44.21

Gene sequence:

>285_bases
ATGAACAAAGCAGAACTTATAGAGGCTTTAGCAGACAAGACCGGGTTACAAAAGCAAGAAGCAAAGAAAGTATTGGATGC
TTACATCGAGATCGTAACTGAGCGTATGTCTGAAAATGAGGAAATCGTACTTGTCGGATTTGGAACACTGATCCCAAGAC
CGCAAACACAGCGTCTTGCCAGAAATCCTAAAACAGGTACTCCGGTAATGATCCCCGCTCGTACGACCGTAAAATTCAAA
CCGGGTAAATTCCTCCTTGAGGCGATGAATGCCCACCTGAAATAA

Upstream 100 bases:

>100_bases
TAAGAAACTCAAATGCAAATGTATCTTTGCAAAACGTAACTATAAAACCAGCTTCAAAGTGTACATTTGTAGTGTTTAAC
AATTTAAAAAATTTGCATTT

Downstream 100 bases:

>100_bases
TAAAGTCACCGAAAACGTAGTAAAAATATCCCCGGTAAGGGGATAAAAAATCATAAGTATGAAATCTTTCATTTATAAGT
ATGAATGAAAAATTTCATAC

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MNKAELIEALADKTGLQKQEAKKVLDAYIEIVTERMSENEEIVLVGFGTLIPRPQTQRLARNPKTGTPVMIPARTTVKFK
PGKFLLEAMNAHLK

Sequences:

>Translated_94_residues
MNKAELIEALADKTGLQKQEAKKVLDAYIEIVTERMSENEEIVLVGFGTLIPRPQTQRLARNPKTGTPVMIPARTTVKFK
PGKFLLEAMNAHLK
>Mature_94_residues
MNKAELIEALADKTGLQKQEAKKVLDAYIEIVTERMSENEEIVLVGFGTLIPRPQTQRLARNPKTGTPVMIPARTTVKFK
PGKFLLEAMNAHLK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=40, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1788005, Length=85, Percent_Identity=41.1764705882353, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10518; Mature: 10518

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAELIEALADKTGLQKQEAKKVLDAYIEIVTERMSENEEIVLVGFGTLIPRPQTQRLA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
RNPKTGTPVMIPARTTVKFKPGKFLLEAMNAHLK
CCCCCCCCEEECCCCEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MNKAELIEALADKTGLQKQEAKKVLDAYIEIVTERMSENEEIVLVGFGTLIPRPQTQRLA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
RNPKTGTPVMIPARTTVKFKPGKFLLEAMNAHLK
CCCCCCCCEEECCCCEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10722605; 10761919 [H]