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The map label for this gene is lipB [H]

Identifier: 150007789

GI number: 150007789

Start: 1327151

End: 1327906

Strand: Reverse

Name: lipB [H]

Synonym: BDI_1147

Alternate gene names: 150007789

Gene position: 1327906-1327151 (Counterclockwise)

Preceding gene: 150007790

Following gene: 150007788

Centisome position: 27.6

GC content: 45.37

Gene sequence:

>756_bases
ATGAAAATCGTTTTTTTAGGAACAGGAACGTCTACGGGCAATCCTGAGATAGGTTGCCAATGTGAGGTATGTACTAGTAA
AGACCCCCGTGATTGGCGTCTACGGGCCTCGATCTTGGTAGAGACGGAAGGGAAACGGATATTGATCGATTGTGGTCCGG
ACTTCCGTTGGCAAATGATAACCAATAAGATTTACCATTTTGACGCCGTTTTAGTAACCCATGAGCACTACGATCATGTA
GGTGGCTTAGATGATCTACGTCCTTTTGGTCGGTATAAAGATGTGGATATTTATGCGGAAGACAATGTCGTGGAGGCGAT
AAAAACTCGCATTCCTTATGTGTTCCGGGAGCATAAATATCCGGGAGTGCCTAATTTGGTATTGCATACGATCGGGACGA
AACCGTTTGAGGCCGCCGGTGTCATGATCACTCCCATTCGTGTAATGCACGCTAAGCTGCCGATCTTAGGTTTCCGTATC
GGCAATATGGCTTACTTGACCGACCTTAAATATCTTCCGGAAGAGGAGTACGCTAAGTTGGAAAACCTTGATGTATTGGT
TATTGATGCTCTTCGGAAAGGAGAGCATCAATCGCATGAGAGTCTGGAGGAAGCCTTAGCGAATATATCCCGTATCCAGC
CCAAGGAGGCCTATTTAATCCATATGAGTCATCGCATAGGGTTGCATGCGGTCGTTGAAAAAGAGTTGCCTCCGCATGTA
CATTACTCATATGATGGATTAACGGTGACTTTTTAA

Upstream 100 bases:

>100_bases
CCCGGGGGGATGAGATCGCGTTGGTCGCGGAGAGCATTCGTACCGCCGTAAAAGATCGTTTCGGTATAGAGCTTATGCCT
GAGGTTAAATATGTCGGATA

Downstream 100 bases:

>100_bases
GCCATATATTAAAAAGATTCTTTATCTTTGCCAGCTGAAAATCTAGAGAATAGCTGCTTTTTTTAAGTTAGTCGCTTATA
GCAGGGGTGGTATATAATAA

Product: putative hydrolase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKIVFLGTGTSTGNPEIGCQCEVCTSKDPRDWRLRASILVETEGKRILIDCGPDFRWQMITNKIYHFDAVLVTHEHYDHV
GGLDDLRPFGRYKDVDIYAEDNVVEAIKTRIPYVFREHKYPGVPNLVLHTIGTKPFEAAGVMITPIRVMHAKLPILGFRI
GNMAYLTDLKYLPEEEYAKLENLDVLVIDALRKGEHQSHESLEEALANISRIQPKEAYLIHMSHRIGLHAVVEKELPPHV
HYSYDGLTVTF

Sequences:

>Translated_251_residues
MKIVFLGTGTSTGNPEIGCQCEVCTSKDPRDWRLRASILVETEGKRILIDCGPDFRWQMITNKIYHFDAVLVTHEHYDHV
GGLDDLRPFGRYKDVDIYAEDNVVEAIKTRIPYVFREHKYPGVPNLVLHTIGTKPFEAAGVMITPIRVMHAKLPILGFRI
GNMAYLTDLKYLPEEEYAKLENLDVLVIDALRKGEHQSHESLEEALANISRIQPKEAYLIHMSHRIGLHAVVEKELPPHV
HYSYDGLTVTF
>Mature_251_residues
MKIVFLGTGTSTGNPEIGCQCEVCTSKDPRDWRLRASILVETEGKRILIDCGPDFRWQMITNKIYHFDAVLVTHEHYDHV
GGLDDLRPFGRYKDVDIYAEDNVVEAIKTRIPYVFREHKYPGVPNLVLHTIGTKPFEAAGVMITPIRVMHAKLPILGFRI
GNMAYLTDLKYLPEEEYAKLENLDVLVIDALRKGEHQSHESLEEALANISRIQPKEAYLIHMSHRIGLHAVVEKELPPHV
HYSYDGLTVTF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=261, Percent_Identity=27.9693486590038, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28645; Mature: 28645

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFLGTGTSTGNPEIGCQCEVCTSKDPRDWRLRASILVETEGKRILIDCGPDFRWQMI
CEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEEEEE
TNKIYHFDAVLVTHEHYDHVGGLDDLRPFGRYKDVDIYAEDNVVEAIKTRIPYVFREHKY
CCEEEEEEEEEEEECCHHCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHCCEEEECCCC
PGVPNLVLHTIGTKPFEAAGVMITPIRVMHAKLPILGFRIGNMAYLTDLKYLPEEEYAKL
CCCCHHEEEECCCCCCCCCCEEEECCEEEHHCCCEEEEEECCEEEEECCCCCCCHHHHHH
ENLDVLVIDALRKGEHQSHESLEEALANISRIQPKEAYLIHMSHRIGLHAVVEKELPPHV
CCCCEEEEECHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCE
HYSYDGLTVTF
EEEECCEEEEC
>Mature Secondary Structure
MKIVFLGTGTSTGNPEIGCQCEVCTSKDPRDWRLRASILVETEGKRILIDCGPDFRWQMI
CEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCEEEEECCCCCEEEEE
TNKIYHFDAVLVTHEHYDHVGGLDDLRPFGRYKDVDIYAEDNVVEAIKTRIPYVFREHKY
CCEEEEEEEEEEEECCHHCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHCCEEEECCCC
PGVPNLVLHTIGTKPFEAAGVMITPIRVMHAKLPILGFRIGNMAYLTDLKYLPEEEYAKL
CCCCHHEEEECCCCCCCCCCEEEECCEEEHHCCCEEEEEECCEEEEECCCCCCCHHHHHH
ENLDVLVIDALRKGEHQSHESLEEALANISRIQPKEAYLIHMSHRIGLHAVVEKELPPHV
CCCCEEEEECHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCE
HYSYDGLTVTF
EEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]