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The map label for this gene is ykfB [H]

Identifier: 150007780

GI number: 150007780

Start: 1313557

End: 1314615

Strand: Direct

Name: ykfB [H]

Synonym: BDI_1138

Alternate gene names: 150007780

Gene position: 1313557-1314615 (Clockwise)

Preceding gene: 150007779

Following gene: 150007781

Centisome position: 27.3

GC content: 54.11

Gene sequence:

>1059_bases
ATGGTGAAGGAGAGGTTTTATAAGACAGAGGTGGTTCCCCGGTGCTTGACTTTCAAGCGACCGGCAGGAACCTCACGGGG
GGTTTACACGACCCGGAAATTGTGGGAGGTCCGAATCCGCAAGGAAGACGAACCCTCGGTTATTGGTATCGGAGAATGTG
CCCCCCTGCCGGATCTCAGTTGCGATTATGGAGTGGATTACGAGATCACGTTATCCAAGGCTTGCCTCGATCTGGAACAG
AAGGGGCATGTTGATACCGAATCTTTGCGCCATTACCCATCCATCCTTTTCGGGCTGGAGACGGCCATGCGCCATTATGA
ACAGGGCGGTTGGCGGCATTACGACACGCCCTTCTCGCAGGGGCAGGCGGGCATCCCCATCAACGGACTGATCTGGATGG
GCGATTATAAGTACATGCTGGAACAGGTAGAGGAAAAGATGAAGGAGGGCTTCCGCTGCGTCAAGCTGAAGATCGGCGCT
ATCGATTTCGACCGGGAACTCTCTTTATTGAAACACATCCGTGCGCATTTCTCTGCCAAGGAAATAGAGCTTCGGGTAGA
TGCCAACGGCGCTTTCACACCCTCGGAGGCGATGGACAAACTGAAGCGGCTCGCCGAGATGGACTTGCATTCCATCGAGC
AGCCTATCCGTGCCGGGCAGTTGGAAGAGATGGCACGCCTTGCGGCAGAGTCACCCCTTCCCATCGCTCTCGACGAGGAA
CTGATCGGCTGTAACGCCCCGGACGAGAAACGGCGGTTGTTGGAAACGATCCATCCCCAATACATTATATTAAAGCCTTC
CCTCCACGGAGGTATCTCCGGATGTACGGAATGGATTCACTTAGCGGAAACTATCTTAGGGAGTACCCCTGAACAACCTA
AATGGTGGGTCACCTCCGCTCTGGAATCGCAAATCGGACTGAACGCTATCGCCCAATGGTGCGCCACGCTAGGTAACCCG
CTACCGCAAGGTCTGGGAACCGGCGAGATTTTCAGGGATGATAAACATACGTTGATCCAACGAAAAGGAGACACCATCTG
GTTCACTAACGACCTTTGA

Upstream 100 bases:

>100_bases
AGGCATTCTTGGAGAAACGCCGTCCCCGCTTCGAGGATTACCCGAAGTTCCCGTAAAGGGAATTGAGAATTGAGAATTGA
GAATTGAAAATTGAAAATGA

Downstream 100 bases:

>100_bases
CCTATGCCGATACAAGAAGATTTACAGGCATTCTTAGCTGAATGGCACGATCCCTCCCCTTTTCTGGAAGTCCAGACCTC
CGGCTCCACCGGTACGCCCA

Product: putative muconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEPSVIGIGECAPLPDLSCDYGVDYEITLSKACLDLEQ
KGHVDTESLRHYPSILFGLETAMRHYEQGGWRHYDTPFSQGQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGA
IDFDRELSLLKHIRAHFSAKEIELRVDANGAFTPSEAMDKLKRLAEMDLHSIEQPIRAGQLEEMARLAAESPLPIALDEE
LIGCNAPDEKRRLLETIHPQYIILKPSLHGGISGCTEWIHLAETILGSTPEQPKWWVTSALESQIGLNAIAQWCATLGNP
LPQGLGTGEIFRDDKHTLIQRKGDTIWFTNDL

Sequences:

>Translated_352_residues
MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEPSVIGIGECAPLPDLSCDYGVDYEITLSKACLDLEQ
KGHVDTESLRHYPSILFGLETAMRHYEQGGWRHYDTPFSQGQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGA
IDFDRELSLLKHIRAHFSAKEIELRVDANGAFTPSEAMDKLKRLAEMDLHSIEQPIRAGQLEEMARLAAESPLPIALDEE
LIGCNAPDEKRRLLETIHPQYIILKPSLHGGISGCTEWIHLAETILGSTPEQPKWWVTSALESQIGLNAIAQWCATLGNP
LPQGLGTGEIFRDDKHTLIQRKGDTIWFTNDL
>Mature_352_residues
MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEPSVIGIGECAPLPDLSCDYGVDYEITLSKACLDLEQ
KGHVDTESLRHYPSILFGLETAMRHYEQGGWRHYDTPFSQGQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGA
IDFDRELSLLKHIRAHFSAKEIELRVDANGAFTPSEAMDKLKRLAEMDLHSIEQPIRAGQLEEMARLAAESPLPIALDEE
LIGCNAPDEKRRLLETIHPQYIILKPSLHGGISGCTEWIHLAETILGSTPEQPKWWVTSALESQIGLNAIAQWCATLGNP
LPQGLGTGEIFRDDKHTLIQRKGDTIWFTNDL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39889; Mature: 39889

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEPSVIGIGECAPLPDLS
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCEEEECCCCCCCCCC
CDYGVDYEITLSKACLDLEQKGHVDTESLRHYPSILFGLETAMRHYEQGGWRHYDTPFSQ
CCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGAIDFDRELSLLKHIRAHFSAK
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
EIELRVDANGAFTPSEAMDKLKRLAEMDLHSIEQPIRAGQLEEMARLAAESPLPIALDEE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCH
LIGCNAPDEKRRLLETIHPQYIILKPSLHGGISGCTEWIHLAETILGSTPEQPKWWVTSA
HHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LESQIGLNAIAQWCATLGNPLPQGLGTGEIFRDDKHTLIQRKGDTIWFTNDL
HHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEPSVIGIGECAPLPDLS
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCEEEECCCCCCCCCC
CDYGVDYEITLSKACLDLEQKGHVDTESLRHYPSILFGLETAMRHYEQGGWRHYDTPFSQ
CCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGAIDFDRELSLLKHIRAHFSAK
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
EIELRVDANGAFTPSEAMDKLKRLAEMDLHSIEQPIRAGQLEEMARLAAESPLPIALDEE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCH
LIGCNAPDEKRRLLETIHPQYIILKPSLHGGISGCTEWIHLAETILGSTPEQPKWWVTSA
HHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LESQIGLNAIAQWCATLGNPLPQGLGTGEIFRDDKHTLIQRKGDTIWFTNDL
HHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]