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The map label for this gene is menD

Identifier: 150007778

GI number: 150007778

Start: 1310996

End: 1312684

Strand: Direct

Name: menD

Synonym: BDI_1136

Alternate gene names: 150007778

Gene position: 1310996-1312684 (Clockwise)

Preceding gene: 150007777

Following gene: 150007779

Centisome position: 27.25

GC content: 50.74

Gene sequence:

>1689_bases
ATGTATAGCGATAAAAAGAACATCCTTCAATTGGTAGCCTTGCTGATAGAGCACGGCGTAAGTAATATCGTGCTATGTCC
GGGGAGCCGGAACTCTCCGATTGTGCACACATTGGCAAACCATTCGTTCTTCAATTGCCATTCCGTGACGGATGAGCGAA
GCGCGGGATTCTTTGCCGTAGGGCTAGCGTTGCACGGAGGTAAGCCGGCGGCTGTTTGCTGTACCTCGGGCACGGCCTTG
CTGAATATACATCCGGCGGTAGCGGAGGCTTTCTATCAGAAAGTTCCGCTTGTCGTGATATCCGCCGACCGCCCCGCCGC
ATGGATCGGGCAAATGGACGGACAGACCTTGCCGCAACCCGGGGTGTTCGGCTCGCTGGTGAAGAAATCGGTACAGCTAC
CGGAAATACATACGGATCAAGATGAATGGTATTGTAATCGATTGATCAACGAGGCTTTGTTGGAACTGAACCATCACGGC
AAAGGGCCGGTTCATATCAACGTCCCGATCTCCGAGCCTTTATTTCAGTTCACGACACCCGAATTGCCGAAGGTACGGGT
AATCACCCGTTATCAAGGCCTAAACGTGTACGACCGGAAATATGACGATCTGATCGAGCGTTTGAACAAATATAGCAAGC
GAATGATGATAGCGGGACAAATGAGCTTGATCTATCTCTTCGAGAAGAAAATATGTAAGTTACTATACAAACATTTCACT
TGGCTCTCCGAGCACATCGGCAACCGTATCGTACCGGGATTGCCGATCAAGAATTTCGACGCCATCCTGTACGCTACCCC
AGAGGAGGAAAAAGAGAAGCTGGTTCCCGAGCTCGTGATCACCTACGGCGGCCATATTGTCTCCAAGCGGCTGAAAGAGT
TTTTGCGGAAACACCCGCCTAAGGAGCATTGGCATGTCTCATTGGATGGGGAGGTAGCGGATTTATTCGGTAGCCTGACC
ACGGTGATCGAGATGGACGCTTTCGAGTTTCTGGAGAAAATCGCTTATATGCTGGAGAATAGGCAGATTGAATATCCGAA
GGCTTGGGAATATAAATCCCGGAATCTGCCGGAGCCGGAGTTCGCTTACTCCGAAATGGCGGCGATCGGTGGTTTGATAC
GTTCGCTTCCGGCTGAATCGGCCTTGCATCTGGGGAATAGCTCCACCGTACGTTACGCACAATTGTACACCGTACCCGAG
ACGGTTGAGGTTTGTTGTAACCGAGGTACGAGCGGGATCGAAGGCTCCTTGTCTACGGCTATCGGATACGCCTCGGCCTC
GCAAAAACTAAACTTTATCGTTATAGGCGATCTTAGTTTCTTCTATGATATGAATGCGTTGTGGAACGGAAACTTGCACA
ATAACCTTCGTATCCTGCTCTTGAACAACGGAGGTGGTGAGATCTTCCAAGCCTTGCCAGGATTCAAGATGAACGAGCGT
ACGCACCGTTACGTCACCGCCTCCCACCAGACCTCAGCGGAGGGATGGGCCACGGAAAGGGGATTCTCCTACTCCGCCGT
TCATAACGCCGAGGAGTTGGCAGCTGCGATGAGCGCCTTTACCCAAACGGAGCAACCGAGCGAACATCCCCTATTCATGG
AGGTTTTCACGGACAAAGCGGAAGACGTACGATTGCTCAAGGAATATTATCATCAACTAAAAAATTCATCCCAAGCACAA
AACATATAA

Upstream 100 bases:

>100_bases
TGTTGGCGGCCTCGCAGCTAGAGGATGAGTGGCAGGAGACAGAGGATAAATTAGATACGATGCGGAGAATAGTGGGTAAT
TTATAATTTTATATCATATA

Downstream 100 bases:

>100_bases
TATATGGAAACGAAAAGAGAATGGACAACCATCAAGGAATACGAGGAGATCTTATTTGATTTCTACAACGGCATCGCCCG
CATTACGATAAACCGGGAAC

Product: 2-oxoglutarate decarboxylase and 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSAGFFAVGLALHGGKPAAVCCTSGTAL
LNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHG
KGPVHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKICKLLYKHFT
WLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLT
TVIEMDAFEFLEKIAYMLENRQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVPE
TVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEIFQALPGFKMNER
THRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQ
NI

Sequences:

>Translated_562_residues
MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSAGFFAVGLALHGGKPAAVCCTSGTAL
LNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHG
KGPVHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKICKLLYKHFT
WLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLT
TVIEMDAFEFLEKIAYMLENRQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVPE
TVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEIFQALPGFKMNER
THRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQ
NI
>Mature_562_residues
MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSAGFFAVGLALHGGKPAAVCCTSGTAL
LNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHG
KGPVHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKICKLLYKHFT
WLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLT
TVIEMDAFEFLEKIAYMLENRQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVPE
TVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEIFQALPGFKMNER
THRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQ
NI

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=500, Percent_Identity=31.4, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PARD8 (A6LB35)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302521.1
- ProteinModelPortal:   A6LB35
- STRING:   A6LB35
- GeneID:   5306289
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1136
- eggNOG:   COG1165
- HOGENOM:   HBG443204
- OMA:   FYHDLNG
- ProtClustDB:   CLSK2757504
- BioCyc:   PDIS435591:BDI_1136-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63158; Mature: 63158

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSAGFFAV
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEE
GLALHGGKPAAVCCTSGTALLNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDGQTLPQP
EEEEECCCCEEEEEECCCEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCCCCCC
GVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHGKGPVHINVPISEPLFQFTTP
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCC
ELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKICKLLYKHFT
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHH
WLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRLKEFLRKHPP
HHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
KEHWHVSLDGEVADLFGSLTTVIEMDAFEFLEKIAYMLENRQIEYPKAWEYKSRNLPEPE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
FAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVPETVEVCCNRGTSGIEGSLSTA
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEECHHHHHHHHCCCCCCCCCCHHHH
IGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEIFQALPGFKMNER
HHHHHHCCCEEEEEEECHHHHHCCCHHCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCC
THRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPSEHPLFMEVFTDKA
CCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCH
EDVRLLKEYYHQLKNSSQAQNI
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFNCHSVTDERSAGFFAV
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEE
GLALHGGKPAAVCCTSGTALLNIHPAVAEAFYQKVPLVVISADRPAAWIGQMDGQTLPQP
EEEEECCCCEEEEEECCCEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCCCCCC
GVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHGKGPVHINVPISEPLFQFTTP
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCC
ELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMSLIYLFEKKICKLLYKHFT
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHH
WLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVITYGGHIVSKRLKEFLRKHPP
HHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
KEHWHVSLDGEVADLFGSLTTVIEMDAFEFLEKIAYMLENRQIEYPKAWEYKSRNLPEPE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
FAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVPETVEVCCNRGTSGIEGSLSTA
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEECHHHHHHHHCCCCCCCCCCHHHH
IGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRILLLNNGGGEIFQALPGFKMNER
HHHHHHCCCEEEEEEECHHHHHCCCHHCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCC
THRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAMSAFTQTEQPSEHPLFMEVFTDKA
CCEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCH
EDVRLLKEYYHQLKNSSQAQNI
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA