Click here to switch to the map view.

The map label for this gene is guaD [H]

Identifier: 150007770

GI number: 150007770

Start: 1301519

End: 1302859

Strand: Reverse

Name: guaD [H]

Synonym: BDI_1128

Alternate gene names: 150007770

Gene position: 1302859-1301519 (Counterclockwise)

Preceding gene: 150007771

Following gene: 150007767

Centisome position: 27.08

GC content: 49.07

Gene sequence:

>1341_bases
TTGACCATGATGAAGAACTTATATAGGGGAGAAGTGCTCTATTTGATCGCTTCCCCGCTGGATCATGTGGAGGCTTACCG
CTATTTTGAGGATGGGGGACTGGCTGTTGTTGATGGAAAGATTGTAGAGGCGGGGCCGTTCGAGGCGATGCGTTCCCGTT
ACCCGGATTTCCCGGTGACAGACTATTCCGGTAAATTGATTACACCCGGCTTTATTGATTCCCATATCCATTTCTCCCAG
TCGGAGATACAGGGAATGTATGGCAAGCAACTTCTGGATTGGCTGGACGAATATACGTTTCCCGCCGAGGAAGCTTTCTC
TTCCATGGAATATGCACAAGATATCGCTCGCTTTTTCGTGAAGGAACTGGTAAAGAACGGAACTACGACTTGTGCCGCTT
ATGCCACGGTTCATCCTGCTTCCGTAACCGCATTATTTTCCGTGGCTACGGAGTATAATATGTGTATCTTGGCTGGAAAG
GTGATGATGAACCGGAACGCTCCGGATTATCTGATGGATACCACGCATCAGGGTGAGTTGGACTGCCGTAGCTTAATCGA
GGATTGGGACGGGAAAGGACGGAATCACTATGTGATCACGCCCCGTTTCGCCATAACCAGTACGCCGGATCAATTAAAGT
CTGCCGGTCGTTTACATGCCGAATATCCCAGTACCTATATACAGACACATCTTTCGGAAAATCTGGGAGAGATTGAATCT
GTCTTATCGCTCTGTCCCGGTCATGCGGATTATCTGGAGGTCTATGAGCGTGCCGGATTGTTGACCGATCGTTCGATATT
CGGGCATTGTGTCCATCTAACCGATCGGGAATATAAGCGGTTGGGAGAAACGGGAGGCATTATCGCCCATTGCCCGACCT
CGAACCTTTTCCTTGGAAGCGGATTATTCGATATGAGGGAAGCGAACCGTTACGGCGTACGTACTACCTTGGCGACAGAT
GTCGGTGCGGGTACATCTTTCTCTATGTGGCGTACGATGGGAGAGGCCTATAAGGTACAACAGTTGAACGGTTATCCGAT
GACAGCATTGGAGGCTATCTATAAATGTACGCTAGGCTCTGCACGGGCCTTATCGTTGGACGACCGAATCGGAAGTTTCC
TCCCTGGCCGTGAAGCGGATTTCATAGTCGTGGATTATGCCGCTACCTCTGTCCAGAAATTGCGTATGGAGTATCTTCGT
TCGAGAGATAAATGGACGATCGAGAATAAGCTGTTTGGCCTGCAAACCCTCGGCGATGACCGGAATACGGTATGTACTTA
TATAATGGGCAAGCAAGTATACGGCTCAGATTCGTGCGATCATCAAGAAGCAAGTATGTAA

Upstream 100 bases:

>100_bases
TGACTTGACGAAAGAGATCGAACAGAATCTGCGTGATAAGTTCGGTCAGGCTACGCACATCGCTTTGCACGTGGAACCAA
TTAAATAAACGAATTGTTAT

Downstream 100 bases:

>100_bases
TACGACCGTTACGACGATCCATAGGCGCTCGGCAGTAGTCGGGCGCAATCCCGGGAAATGATATTCTATAGCCCCGTGCT
TGCAAGCGGAGAGGCAATCT

Product: guanine aminohydrolase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTMMKNLYRGEVLYLIASPLDHVEAYRYFEDGGLAVVDGKIVEAGPFEAMRSRYPDFPVTDYSGKLITPGFIDSHIHFSQ
SEIQGMYGKQLLDWLDEYTFPAEEAFSSMEYAQDIARFFVKELVKNGTTTCAAYATVHPASVTALFSVATEYNMCILAGK
VMMNRNAPDYLMDTTHQGELDCRSLIEDWDGKGRNHYVITPRFAITSTPDQLKSAGRLHAEYPSTYIQTHLSENLGEIES
VLSLCPGHADYLEVYERAGLLTDRSIFGHCVHLTDREYKRLGETGGIIAHCPTSNLFLGSGLFDMREANRYGVRTTLATD
VGAGTSFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGSARALSLDDRIGSFLPGREADFIVVDYAATSVQKLRMEYLR
SRDKWTIENKLFGLQTLGDDRNTVCTYIMGKQVYGSDSCDHQEASM

Sequences:

>Translated_446_residues
MTMMKNLYRGEVLYLIASPLDHVEAYRYFEDGGLAVVDGKIVEAGPFEAMRSRYPDFPVTDYSGKLITPGFIDSHIHFSQ
SEIQGMYGKQLLDWLDEYTFPAEEAFSSMEYAQDIARFFVKELVKNGTTTCAAYATVHPASVTALFSVATEYNMCILAGK
VMMNRNAPDYLMDTTHQGELDCRSLIEDWDGKGRNHYVITPRFAITSTPDQLKSAGRLHAEYPSTYIQTHLSENLGEIES
VLSLCPGHADYLEVYERAGLLTDRSIFGHCVHLTDREYKRLGETGGIIAHCPTSNLFLGSGLFDMREANRYGVRTTLATD
VGAGTSFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGSARALSLDDRIGSFLPGREADFIVVDYAATSVQKLRMEYLR
SRDKWTIENKLFGLQTLGDDRNTVCTYIMGKQVYGSDSCDHQEASM
>Mature_445_residues
TMMKNLYRGEVLYLIASPLDHVEAYRYFEDGGLAVVDGKIVEAGPFEAMRSRYPDFPVTDYSGKLITPGFIDSHIHFSQS
EIQGMYGKQLLDWLDEYTFPAEEAFSSMEYAQDIARFFVKELVKNGTTTCAAYATVHPASVTALFSVATEYNMCILAGKV
MMNRNAPDYLMDTTHQGELDCRSLIEDWDGKGRNHYVITPRFAITSTPDQLKSAGRLHAEYPSTYIQTHLSENLGEIESV
LSLCPGHADYLEVYERAGLLTDRSIFGHCVHLTDREYKRLGETGGIIAHCPTSNLFLGSGLFDMREANRYGVRTTLATDV
GAGTSFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGSARALSLDDRIGSFLPGREADFIVVDYAATSVQKLRMEYLRS
RDKWTIENKLFGLQTLGDDRNTVCTYIMGKQVYGSDSCDHQEASM

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=382, Percent_Identity=32.9842931937173, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1789249, Length=425, Percent_Identity=49.8823529411765, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319963, Length=416, Percent_Identity=30.7692307692308, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24643849, Length=449, Percent_Identity=32.9621380846325, Blast_Score=208, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50011; Mature: 49880

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMMKNLYRGEVLYLIASPLDHVEAYRYFEDGGLAVVDGKIVEAGPFEAMRSRYPDFPVT
CCHHHHHHCCCEEEEEECCHHHHHHHHEECCCCEEEECCEEECCCCHHHHHHCCCCCCCC
DYSGKLITPGFIDSHIHFSQSEIQGMYGKQLLDWLDEYTFPAEEAFSSMEYAQDIARFFV
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KELVKNGTTTCAAYATVHPASVTALFSVATEYNMCILAGKVMMNRNAPDYLMDTTHQGEL
HHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCEEEEECHHHCCCCCCCHHHCCCCCCCH
DCRSLIEDWDGKGRNHYVITPRFAITSTPDQLKSAGRLHAEYPSTYIQTHLSENLGEIES
HHHHHHHHHCCCCCCEEEEECCEEECCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHH
VLSLCPGHADYLEVYERAGLLTDRSIFGHCVHLTDREYKRLGETGGIIAHCPTSNLFLGS
HHHHCCCCHHHHHHHHHCCCCCCCHHHHEEEECCCHHHHHHCCCCCEEEECCCCCEEECC
GLFDMREANRYGVRTTLATDVGAGTSFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGS
CCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
ARALSLDDRIGSFLPGREADFIVVDYAATSVQKLRMEYLRSRDKWTIENKLFGLQTLGDD
CEEEECHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHEEEHHCCCCC
RNTVCTYIMGKQVYGSDSCDHQEASM
CCCEEEEEECCEECCCCCCCCCCCCC
>Mature Secondary Structure 
TMMKNLYRGEVLYLIASPLDHVEAYRYFEDGGLAVVDGKIVEAGPFEAMRSRYPDFPVT
CHHHHHHCCCEEEEEECCHHHHHHHHEECCCCEEEECCEEECCCCHHHHHHCCCCCCCC
DYSGKLITPGFIDSHIHFSQSEIQGMYGKQLLDWLDEYTFPAEEAFSSMEYAQDIARFFV
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KELVKNGTTTCAAYATVHPASVTALFSVATEYNMCILAGKVMMNRNAPDYLMDTTHQGEL
HHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCEEEEECHHHCCCCCCCHHHCCCCCCCH
DCRSLIEDWDGKGRNHYVITPRFAITSTPDQLKSAGRLHAEYPSTYIQTHLSENLGEIES
HHHHHHHHHCCCCCCEEEEECCEEECCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHH
VLSLCPGHADYLEVYERAGLLTDRSIFGHCVHLTDREYKRLGETGGIIAHCPTSNLFLGS
HHHHCCCCHHHHHHHHHCCCCCCCHHHHEEEECCCHHHHHHCCCCCEEEECCCCCEEECC
GLFDMREANRYGVRTTLATDVGAGTSFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGS
CCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
ARALSLDDRIGSFLPGREADFIVVDYAATSVQKLRMEYLRSRDKWTIENKLFGLQTLGDD
CEEEECHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHEEEHHCCCCC
RNTVCTYIMGKQVYGSDSCDHQEASM
CCCEEEEEECCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]