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The map label for this gene is cbiF [H]

Identifier: 150007710

GI number: 150007710

Start: 1235837

End: 1237618

Strand: Reverse

Name: cbiF [H]

Synonym: BDI_1065

Alternate gene names: 150007710

Gene position: 1237618-1235837 (Counterclockwise)

Preceding gene: 150007711

Following gene: 150007709

Centisome position: 25.72

GC content: 47.36

Gene sequence:

>1782_bases
ATGAGTACAATATCAATTATTCCAATATCCGATAGCAGCCGGGTGCTTGCTGAGCGAATTCTTGCTTCTTATCCTGAGGC
GAAGATCTTACCATTCGGCTCTTTCTCAAAAGAGGTTTTTCACGAATCCAGTAGTTTGGTATTTATAGGAGCGATGGGAA
TTTGCGTACGCAGTATCGCTCCCTTCGCGGAAGATAAACATACCGATCCGGCCGTGGTTTGTATAGATAGTACCGGCAAA
TACGTGATTCCTGTCTTATCCGGACATATCGGTGGAGCGAACGATCTTTCCAAAGAGTTAGCGAACCTATTAGGAGCCGA
AGCGATTATTACGACACAAAGCGACAACGCAAACCTATGGGCGCTCGATACATTAGGGAAGAAATACGATTGGACCTTGA
TCGCCAAAGATAGCAATGCCGCCATCTCCACTTTTGTCAATGGAAAACCGACAGCGCTACTCCTTGATATACGAGATAAA
GGAACGGATTATTTGGAGCGTACGGTCCCGTCTCATGTCTCTATCTTTTATTCCTTCGAGGCGATACCGCAGCAAGACTA
TGAGTTATTGATGATCGTCAGTCCCCAACAATACGATACATCCATCCCTACGATCACCTATATACCGAAAGTCCTCCACC
TAGGAATGGGTTGCCGCAAGGATATGCAAGGCGATCCTACGGTCGTTTACGAACATATTAAAGATGTATTGCGTGATAAA
CGCCTATATCCGGAAGCTTTGGCGGATGTAAATACAATCGATTTGAAGAAATGCGAACCGGTGCTGACATTATTGGCTTA
TGGAGTTATGGAATGCCCTTTCCACACCTATACATCGGAGGAGCTGAAAGATATCCCGGTACCCAATCCCTCGGAGAAGG
TACTGGAGGTAACCGAATCCCCAAGTGTATCGGAAGCTTCCGCCATTTATGCGGCTCATGGCGGTCCGCTTTTGGTCGAG
AAGCAAAAAGCGGATTTAGGGAAAGGAAATGAATATACCTTTGCCGTAGCGTTAGATCGGGCGGCCTGCCGCAAGGGACA
TATCGAGATCGTAGGAGCCGGTCCCGGCGATCCGGACTTGATCTCTATCCGCGGACGGCAAATGTTGGAGAAAGCGGATT
TGATCCTGTATGCAGGAAGCCTCGTCCCGAAAGAACTTACCTTATGCGCGAAGGCAGGCGCTACGGTGCGAAGCTCGGCG
GATATGAATCTGGAAGAACAGTTCGCGTTGATGAAAGAGTTTTATGATAAAGGATTGTTTGTCGTTCGTTTGCACACGGG
CGATCCTTGCATCTATGGAGCTATTCAAGAGCAAATGAATTACTTTGATCAATACGGGATGGATTATCATATCACTCCGG
GTATCTCCTCTTTCCAAGCCGCCGCCGCCGCTCTTTATTCTCAGTTCACGATTCCGGAGAAAGTACAAACGATCATCCTC
ACTCGTGGCGAAGGACGTACGCCCATGCCTGAGAAAGAGCAACTGCACAAACTGGCTCAAAGCCAAAGTACGATGTGTAT
TTTCTTAAGCGCCGGTGTCGTAGAGAAGGTCCAAGAGGAATTATCGCGACATTACGCTCCAACCACTCCGGTAGCCGCTT
GCTATAAATTGACATGGAAAGACGAGCGGATCTACCGTGGGCAACTGAAAGATCTCGCGAAAATCGTAAAAGAGAATCAT
CTTACACTGACAACATTACTTGTCGTCGGGGATGCCATCGATAATCGCAAGGGTCTATCCCGCTTATATGCGGATGAATT
CAAACATCTTTTCCGTAAGTAA

Upstream 100 bases:

>100_bases
TAGAGGAAGCGGTCCGCCAAACCGGTCTTCGGATATCCGCCCAAACCCGTATCACGATTGATGATTTTAATACGATTACC
GTGAATGTTATTTATAAATT

Downstream 100 bases:

>100_bases
TGATACTGATCCTTGGAGGAACGACAGAAGGTCGTGTGGCTGTGCGGGTAGCGGACGAGGCAGCCGCTACCTATTACTAT
TCTACGAAAGGAACTTTGCA

Product: precorrin-4 C11-methyltransferase

Products: 2S-adenosyl-L-homocysteine; precorrin; sirohydrochlorin; NADH; Proton; siroheme [C]

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MSTISIIPISDSSRVLAERILASYPEAKILPFGSFSKEVFHESSSLVFIGAMGICVRSIAPFAEDKHTDPAVVCIDSTGK
YVIPVLSGHIGGANDLSKELANLLGAEAIITTQSDNANLWALDTLGKKYDWTLIAKDSNAAISTFVNGKPTALLLDIRDK
GTDYLERTVPSHVSIFYSFEAIPQQDYELLMIVSPQQYDTSIPTITYIPKVLHLGMGCRKDMQGDPTVVYEHIKDVLRDK
RLYPEALADVNTIDLKKCEPVLTLLAYGVMECPFHTYTSEELKDIPVPNPSEKVLEVTESPSVSEASAIYAAHGGPLLVE
KQKADLGKGNEYTFAVALDRAACRKGHIEIVGAGPGDPDLISIRGRQMLEKADLILYAGSLVPKELTLCAKAGATVRSSA
DMNLEEQFALMKEFYDKGLFVVRLHTGDPCIYGAIQEQMNYFDQYGMDYHITPGISSFQAAAAALYSQFTIPEKVQTIIL
TRGEGRTPMPEKEQLHKLAQSQSTMCIFLSAGVVEKVQEELSRHYAPTTPVAACYKLTWKDERIYRGQLKDLAKIVKENH
LTLTTLLVVGDAIDNRKGLSRLYADEFKHLFRK

Sequences:

>Translated_593_residues
MSTISIIPISDSSRVLAERILASYPEAKILPFGSFSKEVFHESSSLVFIGAMGICVRSIAPFAEDKHTDPAVVCIDSTGK
YVIPVLSGHIGGANDLSKELANLLGAEAIITTQSDNANLWALDTLGKKYDWTLIAKDSNAAISTFVNGKPTALLLDIRDK
GTDYLERTVPSHVSIFYSFEAIPQQDYELLMIVSPQQYDTSIPTITYIPKVLHLGMGCRKDMQGDPTVVYEHIKDVLRDK
RLYPEALADVNTIDLKKCEPVLTLLAYGVMECPFHTYTSEELKDIPVPNPSEKVLEVTESPSVSEASAIYAAHGGPLLVE
KQKADLGKGNEYTFAVALDRAACRKGHIEIVGAGPGDPDLISIRGRQMLEKADLILYAGSLVPKELTLCAKAGATVRSSA
DMNLEEQFALMKEFYDKGLFVVRLHTGDPCIYGAIQEQMNYFDQYGMDYHITPGISSFQAAAAALYSQFTIPEKVQTIIL
TRGEGRTPMPEKEQLHKLAQSQSTMCIFLSAGVVEKVQEELSRHYAPTTPVAACYKLTWKDERIYRGQLKDLAKIVKENH
LTLTTLLVVGDAIDNRKGLSRLYADEFKHLFRK
>Mature_592_residues
STISIIPISDSSRVLAERILASYPEAKILPFGSFSKEVFHESSSLVFIGAMGICVRSIAPFAEDKHTDPAVVCIDSTGKY
VIPVLSGHIGGANDLSKELANLLGAEAIITTQSDNANLWALDTLGKKYDWTLIAKDSNAAISTFVNGKPTALLLDIRDKG
TDYLERTVPSHVSIFYSFEAIPQQDYELLMIVSPQQYDTSIPTITYIPKVLHLGMGCRKDMQGDPTVVYEHIKDVLRDKR
LYPEALADVNTIDLKKCEPVLTLLAYGVMECPFHTYTSEELKDIPVPNPSEKVLEVTESPSVSEASAIYAAHGGPLLVEK
QKADLGKGNEYTFAVALDRAACRKGHIEIVGAGPGDPDLISIRGRQMLEKADLILYAGSLVPKELTLCAKAGATVRSSAD
MNLEEQFALMKEFYDKGLFVVRLHTGDPCIYGAIQEQMNYFDQYGMDYHITPGISSFQAAAAALYSQFTIPEKVQTIILT
RGEGRTPMPEKEQLHKLAQSQSTMCIFLSAGVVEKVQEELSRHYAPTTPVAACYKLTWKDERIYRGQLKDLAKIVKENHL
TLTTLLVVGDAIDNRKGLSRLYADEFKHLFRK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=249, Percent_Identity=25.3012048192771, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322922, Length=242, Percent_Identity=28.5123966942149, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.107; 1.3.1.76; 4.99.1.4 [C]

Molecular weight: Translated: 65470; Mature: 65339

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTISIIPISDSSRVLAERILASYPEAKILPFGSFSKEVFHESSSLVFIGAMGICVRSIA
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCEEEEEHHHHHHHHHC
PFAEDKHTDPAVVCIDSTGKYVIPVLSGHIGGANDLSKELANLLGAEAIITTQSDNANLW
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEE
ALDTLGKKYDWTLIAKDSNAAISTFVNGKPTALLLDIRDKGTDYLERTVPSHVSIFYSFE
EEECCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEE
AIPQQDYELLMIVSPQQYDTSIPTITYIPKVLHLGMGCRKDMQGDPTVVYEHIKDVLRDK
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
RLYPEALADVNTIDLKKCEPVLTLLAYGVMECPFHTYTSEELKDIPVPNPSEKVLEVTES
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHEECCC
PSVSEASAIYAAHGGPLLVEKQKADLGKGNEYTFAVALDRAACRKGHIEIVGAGPGDPDL
CCCCCHHEEEEECCCCEEEECCCCCCCCCCCEEEEEEECHHHHCCCCEEEEECCCCCCCE
ISIRGRQMLEKADLILYAGSLVPKELTLCAKAGATVRSSADMNLEEQFALMKEFYDKGLF
EEECCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCEE
VVRLHTGDPCIYGAIQEQMNYFDQYGMDYHITPGISSFQAAAAALYSQFTIPEKVQTIIL
EEEEECCCCEEHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHEEEEEE
TRGEGRTPMPEKEQLHKLAQSQSTMCIFLSAGVVEKVQEELSRHYAPTTPVAACYKLTWK
ECCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHEEEECC
DERIYRGQLKDLAKIVKENHLTLTTLLVVGDAIDNRKGLSRLYADEFKHLFRK
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STISIIPISDSSRVLAERILASYPEAKILPFGSFSKEVFHESSSLVFIGAMGICVRSIA
CEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCEEEEEHHHHHHHHHC
PFAEDKHTDPAVVCIDSTGKYVIPVLSGHIGGANDLSKELANLLGAEAIITTQSDNANLW
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEE
ALDTLGKKYDWTLIAKDSNAAISTFVNGKPTALLLDIRDKGTDYLERTVPSHVSIFYSFE
EEECCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEE
AIPQQDYELLMIVSPQQYDTSIPTITYIPKVLHLGMGCRKDMQGDPTVVYEHIKDVLRDK
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
RLYPEALADVNTIDLKKCEPVLTLLAYGVMECPFHTYTSEELKDIPVPNPSEKVLEVTES
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHEECCC
PSVSEASAIYAAHGGPLLVEKQKADLGKGNEYTFAVALDRAACRKGHIEIVGAGPGDPDL
CCCCCHHEEEEECCCCEEEECCCCCCCCCCCEEEEEEECHHHHCCCCEEEEECCCCCCCE
ISIRGRQMLEKADLILYAGSLVPKELTLCAKAGATVRSSADMNLEEQFALMKEFYDKGLF
EEECCHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCEE
VVRLHTGDPCIYGAIQEQMNYFDQYGMDYHITPGISSFQAAAAALYSQFTIPEKVQTIIL
EEEEECCCCEEHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHEEEEEE
TRGEGRTPMPEKEQLHKLAQSQSTMCIFLSAGVVEKVQEELSRHYAPTTPVAACYKLTWK
ECCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHEEEECC
DERIYRGQLKDLAKIVKENHLTLTTLLVVGDAIDNRKGLSRLYADEFKHLFRK
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2S-Adenosyl-L-methionine; uroporphyrin III; dihydrosirohydrochlorin; NAD; sirohydrochlorin; Fe (II) [C]

Specific reaction: 2S-Adenosyl-L-methionine + uroporphyrin III = 2S-adenosyl-L-homocysteine + precorrin dihydrosirohydrochlorin + NAD = sirohydrochlorin + NADH sirohydrochlorin + Fe (II) = 2 Proton + siroheme [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]