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The map label for this gene is ygcA [C]

Identifier: 150007675

GI number: 150007675

Start: 1192554

End: 1193966

Strand: Reverse

Name: ygcA [C]

Synonym: BDI_1030

Alternate gene names: 150007675

Gene position: 1193966-1192554 (Counterclockwise)

Preceding gene: 150007691

Following gene: 150007674

Centisome position: 24.82

GC content: 45.51

Gene sequence:

>1413_bases
TTGGCAAGAAAGAAGAAACAGTTGCCTATATTAGAAAAGGTAACCATTACGGACGTAGCCGCCGAGGGCAAGGCTATCGC
GAGAGTGAACGATATGGTGGTATTTGTACCCTTCGTCGCTCCGGGAGACGTGGTTGATATTCAACTGACCCGTAAGAAGA
ATAGTTATGCGGAGGGGAAACCTGTATATTTCCATGAGTACTCGGCCAAACGTGCGGAGCCGTTTTGTGAGCACTTCGGT
GTCTGTGGAGGTTGCAAGTGGCAACATCTTCCCTATGAGGAACAACTTTACTATAAGCAAAAACAGGTCTACGATAATCT
TACCCGCATCGGGAAAGTCGAAATGGAAGAGAAACTTCCGATCTTAGGTTCCGAGCGCACGACTTTCTACCGGAACAAGC
TTGAGTTTACTTTCTCAAACAAGAAATGGCTTACGGAGGAAGAGATCCAGTCCGGTGCTTCTTTCGATTGCATGAATGGT
GTCGGGTTTCATATACCGGGGATGTTCGATAAGGTATTGGACATCCATAAATGTTGGTTGCAAGATGATATATCGAACAA
GATCCGTCTTTGCGTCAAGGAATATTGCCTATCTCATGAGGGATATCCTTTCTTCGATTTACGGAACCAAGAGGGATTCG
TTCGTACGTTGATGATCCGTACGGCATCCACTGGGGATTTGATGGTCGTATTGGTTTTCTTCCATGAGGATGTAGAGCGC
AGGGAAGCGTTATTATCTCATCTGGCGGAACGATTCCCGGAAATCACGTCCTTGATGTACGTCATCAACGGCAAATGTAA
TGACACGATCACCGACCAAGAAGTTCTCGTATTCAAGGGAAAAGATCATATCATCGAGGAGATGGAGGGGCTTCAATTCA
AGGTAGGTCCTAAGTCTTTCTATCAGACAAACAGTGAACAGGCTTATAATCTATATAAAGTGGCTAGAGAATTTGCGGGT
CTTACCGGAAATGAGATGGTGTACGATTTATATACCGGGACAGGTACGATCGCAAATTTCGTCTCTCGCCAAGCAAAGAA
GGTTATCGGCATCGAATACGTACCGGAGGCTATCGAGGACGCAAAAGTGAATTCCGCCTTGAACCATATAGAGAACACGC
TTTTTTATGCCGGTGATATGAAAGATATCCTTACGCAGGATTTCATAAACCAACACGGACGTCCGGATGTTATTATTACC
GATCCACCACGTGCCGGTATGCATGATGATGTGATCAACACTATTCTCTTCGCCGAGCCGGAACGTATCGTTTACGTTAG
CTGCAATCCTGCTACGCAAGCCCGCGATTTGAGTTTGTTGAGCGTGAAATACTCCGTCAAGAAAGTCCGTCCGGTCGATA
TGTTTCCTCATACACATCATGTGGAGAACGTGGTTTTGCTTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
AGAACTGTTTGCCGGACTCATTTAAGTATATAGAACGAAGCTCTTTGTTCTTAGCGGAATTGTAAGTATTTTCGCGAAGC
GAATTTAAAACTTAACGACA

Downstream 100 bases:

>100_bases
ACATATAATATAATGTATAGGAAATACGTCCCCTTTCTGCTCTTTTTGTTCTTCTTGTATGGTTGCAACGGTAACAAACA
AGAGGAGAAAGAGGACGTTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MARKKKQLPILEKVTITDVAAEGKAIARVNDMVVFVPFVAPGDVVDIQLTRKKNSYAEGKPVYFHEYSAKRAEPFCEHFG
VCGGCKWQHLPYEEQLYYKQKQVYDNLTRIGKVEMEEKLPILGSERTTFYRNKLEFTFSNKKWLTEEEIQSGASFDCMNG
VGFHIPGMFDKVLDIHKCWLQDDISNKIRLCVKEYCLSHEGYPFFDLRNQEGFVRTLMIRTASTGDLMVVLVFFHEDVER
REALLSHLAERFPEITSLMYVINGKCNDTITDQEVLVFKGKDHIIEEMEGLQFKVGPKSFYQTNSEQAYNLYKVAREFAG
LTGNEMVYDLYTGTGTIANFVSRQAKKVIGIEYVPEAIEDAKVNSALNHIENTLFYAGDMKDILTQDFINQHGRPDVIIT
DPPRAGMHDDVINTILFAEPERIVYVSCNPATQARDLSLLSVKYSVKKVRPVDMFPHTHHVENVVLLEKK

Sequences:

>Translated_470_residues
MARKKKQLPILEKVTITDVAAEGKAIARVNDMVVFVPFVAPGDVVDIQLTRKKNSYAEGKPVYFHEYSAKRAEPFCEHFG
VCGGCKWQHLPYEEQLYYKQKQVYDNLTRIGKVEMEEKLPILGSERTTFYRNKLEFTFSNKKWLTEEEIQSGASFDCMNG
VGFHIPGMFDKVLDIHKCWLQDDISNKIRLCVKEYCLSHEGYPFFDLRNQEGFVRTLMIRTASTGDLMVVLVFFHEDVER
REALLSHLAERFPEITSLMYVINGKCNDTITDQEVLVFKGKDHIIEEMEGLQFKVGPKSFYQTNSEQAYNLYKVAREFAG
LTGNEMVYDLYTGTGTIANFVSRQAKKVIGIEYVPEAIEDAKVNSALNHIENTLFYAGDMKDILTQDFINQHGRPDVIIT
DPPRAGMHDDVINTILFAEPERIVYVSCNPATQARDLSLLSVKYSVKKVRPVDMFPHTHHVENVVLLEKK
>Mature_469_residues
ARKKKQLPILEKVTITDVAAEGKAIARVNDMVVFVPFVAPGDVVDIQLTRKKNSYAEGKPVYFHEYSAKRAEPFCEHFGV
CGGCKWQHLPYEEQLYYKQKQVYDNLTRIGKVEMEEKLPILGSERTTFYRNKLEFTFSNKKWLTEEEIQSGASFDCMNGV
GFHIPGMFDKVLDIHKCWLQDDISNKIRLCVKEYCLSHEGYPFFDLRNQEGFVRTLMIRTASTGDLMVVLVFFHEDVERR
EALLSHLAERFPEITSLMYVINGKCNDTITDQEVLVFKGKDHIIEEMEGLQFKVGPKSFYQTNSEQAYNLYKVAREFAGL
TGNEMVYDLYTGTGTIANFVSRQAKKVIGIEYVPEAIEDAKVNSALNHIENTLFYAGDMKDILTQDFINQHGRPDVIITD
PPRAGMHDDVINTILFAEPERIVYVSCNPATQARDLSLLSVKYSVKKVRPVDMFPHTHHVENVVLLEKK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=429, Percent_Identity=29.8368298368298, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI51173878, Length=429, Percent_Identity=29.8368298368298, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI269784678, Length=288, Percent_Identity=28.4722222222222, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI269784680, Length=288, Percent_Identity=28.4722222222222, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI269784676, Length=288, Percent_Identity=28.4722222222222, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI20149688, Length=288, Percent_Identity=28.4722222222222, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1789148, Length=465, Percent_Identity=28.6021505376344, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=26.1194029850746, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790403, Length=208, Percent_Identity=28.3653846153846, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=167, Percent_Identity=36.5269461077844, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=444, Percent_Identity=30.1801801801802, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 53904; Mature: 53773

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKKQLPILEKVTITDVAAEGKAIARVNDMVVFVPFVAPGDVVDIQLTRKKNSYAEGK
CCCCCCCCCCHHEEEEEHHHCCCCEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCC
PVYFHEYSAKRAEPFCEHFGVCGGCKWQHLPYEEQLYYKQKQVYDNLTRIGKVEMEEKLP
CEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHCCC
ILGSERTTFYRNKLEFTFSNKKWLTEEEIQSGASFDCMNGVGFHIPGMFDKVLDIHKCWL
CCCCCCCEEEECEEEEEECCCCCCCHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
QDDISNKIRLCVKEYCLSHEGYPFFDLRNQEGFVRTLMIRTASTGDLMVVLVFFHEDVER
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEEEHHCCHHH
REALLSHLAERFPEITSLMYVINGKCNDTITDQEVLVFKGKDHIIEEMEGLQFKVGPKSF
HHHHHHHHHHHCHHHHHEEHEECCCCCCCCCCCEEEEEECCHHHHHHHCCCEEEECCHHH
YQTNSEQAYNLYKVAREFAGLTGNEMVYDLYTGTGTIANFVSRQAKKVIGIEYVPEAIED
HCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHH
AKVNSALNHIENTLFYAGDMKDILTQDFINQHGRPDVIITDPPRAGMHDDVINTILFAEP
HHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECC
ERIVYVSCNPATQARDLSLLSVKYSVKKVRPVDMFPHTHHVENVVLLEKK
CEEEEEECCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
ARKKKQLPILEKVTITDVAAEGKAIARVNDMVVFVPFVAPGDVVDIQLTRKKNSYAEGK
CCCCCCCCCHHEEEEEHHHCCCCEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCC
PVYFHEYSAKRAEPFCEHFGVCGGCKWQHLPYEEQLYYKQKQVYDNLTRIGKVEMEEKLP
CEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHCCC
ILGSERTTFYRNKLEFTFSNKKWLTEEEIQSGASFDCMNGVGFHIPGMFDKVLDIHKCWL
CCCCCCCEEEECEEEEEECCCCCCCHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
QDDISNKIRLCVKEYCLSHEGYPFFDLRNQEGFVRTLMIRTASTGDLMVVLVFFHEDVER
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEEEHHCCHHH
REALLSHLAERFPEITSLMYVINGKCNDTITDQEVLVFKGKDHIIEEMEGLQFKVGPKSF
HHHHHHHHHHHCHHHHHEEHEECCCCCCCCCCCEEEEEECCHHHHHHHCCCEEEECCHHH
YQTNSEQAYNLYKVAREFAGLTGNEMVYDLYTGTGTIANFVSRQAKKVIGIEYVPEAIED
HCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHH
AKVNSALNHIENTLFYAGDMKDILTQDFINQHGRPDVIITDPPRAGMHDDVINTILFAEP
HHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECC
ERIVYVSCNPATQARDLSLLSVKYSVKKVRPVDMFPHTHHVENVVLLEKK
CEEEEEECCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]