Click here to switch to the map view.

The map label for this gene is proS

Identifier: 150007636

GI number: 150007636

Start: 1151946

End: 1153427

Strand: Direct

Name: proS

Synonym: BDI_0989

Alternate gene names: 150007636

Gene position: 1151946-1153427 (Clockwise)

Preceding gene: 150007634

Following gene: 150007637

Centisome position: 23.94

GC content: 47.98

Gene sequence:

>1482_bases
ATGGCAAAAGAGCTGAAAGAACTTACACCGAGAAGTGTAAACTATTCGCAGTGGTACCAAGATTTGGTAATTAAGGCAGA
TTTGGCGGAGAATTCCGCCGTGCGTGGTTGTATGGTGATCAAGCCTTACGGATATGCTATCTGGGAGAAGATGCAACGTA
TCCTAGACGATATGTTCAAGGAAACCGGTCATGTGAACGCTTATTTCCCCTTGCTGATCCCGAAGTCTTTCTTAAGCAAA
GAAGCTGAGCACGTGGAAGGATTCGCCAAGGAGTGCGCCGTGGTTACTCACTATCGTTTGAAGACAAATCATGACGGTAC
GGGGGTTGTCGTAGATCCGGCAGCTAAACTGGAAGAGGAATTGATCATTCGCCCGACTTCGGAGACCATTATATGGAATA
CATACCGTAACTGGATTCAGTCTTACCGTGACCTGCCTATCTTGTGCAACCAATGGGCGAACGTGATGCGCTGGGAAATG
CGTACTCGCTTGTTCCTTCGTACGGCTGAGTTCTTGTGGCAGGAAGGCCATACCGCTCACGCTACTCGTGAAGAAGCGGA
GATAGAAGCTAAGAAGATGCAAGACGTTTATGCGAACTTCGCAGAGAATTATATGGCAATGCCGGTAATAAAGGGCGTGA
AGTCTGAGAGCGAGCGTTTCGCCGGTGCTTTGGATACCTATACGATCGAGGCGATGATGCAGGATGGAAAAGCTTTGCAG
GCGGGAACTTCCCATTTCTTGGGGCAGAACTTCGGTAAGGCTTTCGACGTTACTTTCATCGACAAGAACGGTAAGAGCGA
CTATGCTTGGGCTACTTCTTGGGGTGTTTCCACACGTTTGATCGGAGCGTTGATTATGTCTCACTCTGATGATAACGGTT
TGGTATTGCCTCCACATTTGGCTCCGATACAGGTTGTGATCGTACCGATCTACCGTAGCGCTGAGCAATTGACGCAGATC
AGCGAGAAGGTCGCTGGTATCGTGGCTAAATTGAAAGCGTTAGGCATTTCCGTGAAATATGATGACGCTGATAACAAGAA
GCCGGGCTGGAAGTTCGCTGAGTACGAGTTAAAAGGTGTTCCCGTGCGTTTGGCTATGGGTGGTCGTGATTTGGAGAATA
ATACGATCGAAGTAATGCGCCGTGATACTTTGGAGAAGGAGACGATCACTTGTGATGGCATTGAGGAATACGTGAAGAAT
CTGTTGGAGGAAATCCAAGCGAATATCTTCAAGAAAGCGTACGATCACCGTGAGGCTAACATCATAAACGTAGATACGTA
TGAGGAATTTAAGGAGAAGATCGAGGATGGCGTCTTCATCATGGCTCACTGGGATGGAACCCCAGAGACAGAGGAATTGA
TCAAGAACGAGACAAAAGCTACGATCCGCTGTATTCCTTTAGCTGGAGATAAGACTCCGGGTAAGTGTATGGTGACAGGT
AAACCGTCGGCTTGTCGGGTACTGTTCGCCAGAGCTTATTGA

Upstream 100 bases:

>100_bases
CTATATATAGTTTTTACAAAGATATACGATTATTTCTTACCTTTGCAACCCATTACTTATTCAGATTAAGGAAAACAGTA
TAATTTAGAAATATAAAGAA

Downstream 100 bases:

>100_bases
TTGTTATGCTAAAAGATGAAATAAAATGGGGGATGCTTTAACCGAAGGGCATCCCTCTCTAAGAAAATGAGCTACCACGA
ATTTATACTTGTCGTTAACT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAKELKELTPRSVNYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAIWEKMQRILDDMFKETGHVNAYFPLLIPKSFLSK
EAEHVEGFAKECAVVTHYRLKTNHDGTGVVVDPAAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVMRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEIEAKKMQDVYANFAENYMAMPVIKGVKSESERFAGALDTYTIEAMMQDGKALQ
AGTSHFLGQNFGKAFDVTFIDKNGKSDYAWATSWGVSTRLIGALIMSHSDDNGLVLPPHLAPIQVVIVPIYRSAEQLTQI
SEKVAGIVAKLKALGISVKYDDADNKKPGWKFAEYELKGVPVRLAMGGRDLENNTIEVMRRDTLEKETITCDGIEEYVKN
LLEEIQANIFKKAYDHREANIINVDTYEEFKEKIEDGVFIMAHWDGTPETEELIKNETKATIRCIPLAGDKTPGKCMVTG
KPSACRVLFARAY

Sequences:

>Translated_493_residues
MAKELKELTPRSVNYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAIWEKMQRILDDMFKETGHVNAYFPLLIPKSFLSK
EAEHVEGFAKECAVVTHYRLKTNHDGTGVVVDPAAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVMRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEIEAKKMQDVYANFAENYMAMPVIKGVKSESERFAGALDTYTIEAMMQDGKALQ
AGTSHFLGQNFGKAFDVTFIDKNGKSDYAWATSWGVSTRLIGALIMSHSDDNGLVLPPHLAPIQVVIVPIYRSAEQLTQI
SEKVAGIVAKLKALGISVKYDDADNKKPGWKFAEYELKGVPVRLAMGGRDLENNTIEVMRRDTLEKETITCDGIEEYVKN
LLEEIQANIFKKAYDHREANIINVDTYEEFKEKIEDGVFIMAHWDGTPETEELIKNETKATIRCIPLAGDKTPGKCMVTG
KPSACRVLFARAY
>Mature_492_residues
AKELKELTPRSVNYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAIWEKMQRILDDMFKETGHVNAYFPLLIPKSFLSKE
AEHVEGFAKECAVVTHYRLKTNHDGTGVVVDPAAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVMRWEMR
TRLFLRTAEFLWQEGHTAHATREEAEIEAKKMQDVYANFAENYMAMPVIKGVKSESERFAGALDTYTIEAMMQDGKALQA
GTSHFLGQNFGKAFDVTFIDKNGKSDYAWATSWGVSTRLIGALIMSHSDDNGLVLPPHLAPIQVVIVPIYRSAEQLTQIS
EKVAGIVAKLKALGISVKYDDADNKKPGWKFAEYELKGVPVRLAMGGRDLENNTIEVMRRDTLEKETITCDGIEEYVKNL
LEEIQANIFKKAYDHREANIINVDTYEEFKEKIEDGVFIMAHWDGTPETEELIKNETKATIRCIPLAGDKTPGKCMVTGK
PSACRVLFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=504, Percent_Identity=39.484126984127, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1786392, Length=164, Percent_Identity=30.4878048780488, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=503, Percent_Identity=39.3638170974155, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71991346, Length=370, Percent_Identity=43.7837837837838, Blast_Score=326, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321808, Length=457, Percent_Identity=40.9190371991247, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24649466, Length=506, Percent_Identity=37.1541501976285, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24649468, Length=506, Percent_Identity=37.1541501976285, Blast_Score=331, Evalue=8e-91,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PARD8 (A6LAP3)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302379.1
- ProteinModelPortal:   A6LAP3
- SMR:   A6LAP3
- STRING:   A6LAP3
- GeneID:   5306143
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0989
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   PDIS435591:BDI_0989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 56021; Mature: 55890

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKELTPRSVNYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAIWEKMQRILDDMFK
CCCCHHHCCCCCCCHHHHHHHHHEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHYRLKTNHDGTGVVVDPAAKLEEE
HCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCHHHCCCC
LIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVMRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATREEAEIEAKKMQDVYANFAENYMAMPVIKGVKSESERFAGALDTYTIEAMMQDGKALQ
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCHHH
AGTSHFLGQNFGKAFDVTFIDKNGKSDYAWATSWGVSTRLIGALIMSHSDDNGLVLPPHL
HCHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEECCCC
APIQVVIVPIYRSAEQLTQISEKVAGIVAKLKALGISVKYDDADNKKPGWKFAEYELKGV
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEECCC
PVRLAMGGRDLENNTIEVMRRDTLEKETITCDGIEEYVKNLLEEIQANIFKKAYDHREAN
CEEEEECCCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
IINVDTYEEFKEKIEDGVFIMAHWDGTPETEELIKNETKATIRCIPLAGDKTPGKCMVTG
EEECCCHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCCCCEEEEEC
KPSACRVLFARAY
CCCHHEEEEEECC
>Mature Secondary Structure 
AKELKELTPRSVNYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAIWEKMQRILDDMFK
CCCHHHCCCCCCCHHHHHHHHHEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHYRLKTNHDGTGVVVDPAAKLEEE
HCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCHHHCCCC
LIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVMRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATREEAEIEAKKMQDVYANFAENYMAMPVIKGVKSESERFAGALDTYTIEAMMQDGKALQ
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCHHH
AGTSHFLGQNFGKAFDVTFIDKNGKSDYAWATSWGVSTRLIGALIMSHSDDNGLVLPPHL
HCHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEECCCC
APIQVVIVPIYRSAEQLTQISEKVAGIVAKLKALGISVKYDDADNKKPGWKFAEYELKGV
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEECCC
PVRLAMGGRDLENNTIEVMRRDTLEKETITCDGIEEYVKNLLEEIQANIFKKAYDHREAN
CEEEEECCCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
IINVDTYEEFKEKIEDGVFIMAHWDGTPETEELIKNETKATIRCIPLAGDKTPGKCMVTG
EEECCCHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCCCCEEEEEC
KPSACRVLFARAY
CCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA