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The map label for this gene is pmbA [H]

Identifier: 150007565

GI number: 150007565

Start: 1053189

End: 1054508

Strand: Reverse

Name: pmbA [H]

Synonym: BDI_0918

Alternate gene names: 150007565

Gene position: 1054508-1053189 (Counterclockwise)

Preceding gene: 150007566

Following gene: 150007561

Centisome position: 21.92

GC content: 50.68

Gene sequence:

>1320_bases
ATGATAACAAACGAAAATAAGAAATTGGCTCAATGGGCCATGGAATATGCCTTGAAGAATGGTTGTCAGTCTTCACGTGT
CACGCTTTACAATGGTTCCAGCAGTTCGTTTGAGATACGGGATATGAAAATAGACCGTCTGCAACAGGCGTCCGAGAACA
GCATGGTGGTTCATTTGTTCGTGGACGGCCGTTACGGCTCCTACTCTACCAATCGCTTGGATAAGAAAGAGCTGGAGAAG
TTCATCAAGGATGGTATCTCCGCCACTCGTTTCCTCGCCGAGGACAAGGCTCGCACGCTGCCGGACGCTTCCTTGTATTA
TAAAGGTGGGGCGGCGGACTTGCGATTGGTCGACCCGAATTTCGACTCCGTGCAACCGGACGACAAGGTAGCGTTGGCCA
TGAGCGTCTGTGAGGAAATCATGGGCAAGGACGACCGGATCATCTCCGCCAACGCTTCCTACAGCGACGAGAAGGACTTT
AAGTACATGGTAGCCAGCAACGGTTTCGAAGGCGAGGCTTCTGGTTCCTCTTATGGCTTGGTGGGGAGTGTCAGCATCCG
TGGGGAGGGTGACGCCCGTCCCGAGTCTTATTGGTACGACTCTTCCTTGTATTATGACGAGCTGGTGAAGACCGGTATCG
GTACGAAAGCGCTGGAACGGGTGCTTCGAAAACTGGGACAGCGGAAAGTGGCGTCCGGTAAATATACCATGATCGTGGAT
AACATGAACTCCAGTCGTCTGCTTTCTCCAGTAATCGGAGCCATTTACGGTTCCTCCATCCAGCAAAAGAACTCTTTCTT
GTTGGATAAGATCGACCAAAAGGTAGCGAGCGACAAGATGACCTTGATAGATGAGCCTCATTTGGTAAAGGCTTCCGGCG
CACGTTATTTCGATAGCGAGGGTGTTGCCACGAAACGTCTTCCGGTATTCGAGCAGGGTATATTGAAAACGTATTATATC
GATACGTATTCCGCCAACAAGATGGGCATGGAACAAACGATCGGCTCTCCCTCGATTCTTACCATGCGGAATGGTGATAA
GGATCTGGACGGATTGATCGCCTCTATCGACAAAGGTATATTGGTGACCGGCTTCAACGGTGGAAACTGCAATAGCACGA
CCGGCGATTTCTCGTATGGTGTGGAAGGTTTCTTGATCGAGAGAGGCAAGCTATCCCAACCGATCTCCGAGATGAACGCC
ACGGGTAATATGCTCTCCCTATGGAGTAACCTAGCGGAAGTCGGAAACGACCCCCGCCTATCCTCCAGCTGGCGAATCCC
TTCCTTGCTTTTCAATGGGGTTGATTTTAGCGGATTATAA

Upstream 100 bases:

>100_bases
GGCACATGGACCTGCGGTAAGGATGGACAGTCTTGCCCGGTAACCTGCGGTATGCCGTCGGCCTTAGTCAGCAAACTTAC
AGTAGGAGGAGAAAACTAAT

Downstream 100 bases:

>100_bases
ATACAAGAAACAACATCCGGAAGAACAAGAATAAAAGAAGCCGGAACCGCTAAACACAGTTCCGGCTTCTTTATTTTTCC
TTACACCAAATTATAGATCG

Product: putative modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MITNENKKLAQWAMEYALKNGCQSSRVTLYNGSSSSFEIRDMKIDRLQQASENSMVVHLFVDGRYGSYSTNRLDKKELEK
FIKDGISATRFLAEDKARTLPDASLYYKGGAADLRLVDPNFDSVQPDDKVALAMSVCEEIMGKDDRIISANASYSDEKDF
KYMVASNGFEGEASGSSYGLVGSVSIRGEGDARPESYWYDSSLYYDELVKTGIGTKALERVLRKLGQRKVASGKYTMIVD
NMNSSRLLSPVIGAIYGSSIQQKNSFLLDKIDQKVASDKMTLIDEPHLVKASGARYFDSEGVATKRLPVFEQGILKTYYI
DTYSANKMGMEQTIGSPSILTMRNGDKDLDGLIASIDKGILVTGFNGGNCNSTTGDFSYGVEGFLIERGKLSQPISEMNA
TGNMLSLWSNLAEVGNDPRLSSSWRIPSLLFNGVDFSGL

Sequences:

>Translated_439_residues
MITNENKKLAQWAMEYALKNGCQSSRVTLYNGSSSSFEIRDMKIDRLQQASENSMVVHLFVDGRYGSYSTNRLDKKELEK
FIKDGISATRFLAEDKARTLPDASLYYKGGAADLRLVDPNFDSVQPDDKVALAMSVCEEIMGKDDRIISANASYSDEKDF
KYMVASNGFEGEASGSSYGLVGSVSIRGEGDARPESYWYDSSLYYDELVKTGIGTKALERVLRKLGQRKVASGKYTMIVD
NMNSSRLLSPVIGAIYGSSIQQKNSFLLDKIDQKVASDKMTLIDEPHLVKASGARYFDSEGVATKRLPVFEQGILKTYYI
DTYSANKMGMEQTIGSPSILTMRNGDKDLDGLIASIDKGILVTGFNGGNCNSTTGDFSYGVEGFLIERGKLSQPISEMNA
TGNMLSLWSNLAEVGNDPRLSSSWRIPSLLFNGVDFSGL
>Mature_439_residues
MITNENKKLAQWAMEYALKNGCQSSRVTLYNGSSSSFEIRDMKIDRLQQASENSMVVHLFVDGRYGSYSTNRLDKKELEK
FIKDGISATRFLAEDKARTLPDASLYYKGGAADLRLVDPNFDSVQPDDKVALAMSVCEEIMGKDDRIISANASYSDEKDF
KYMVASNGFEGEASGSSYGLVGSVSIRGEGDARPESYWYDSSLYYDELVKTGIGTKALERVLRKLGQRKVASGKYTMIVD
NMNSSRLLSPVIGAIYGSSIQQKNSFLLDKIDQKVASDKMTLIDEPHLVKASGARYFDSEGVATKRLPVFEQGILKTYYI
DTYSANKMGMEQTIGSPSILTMRNGDKDLDGLIASIDKGILVTGFNGGNCNSTTGDFSYGVEGFLIERGKLSQPISEMNA
TGNMLSLWSNLAEVGNDPRLSSSWRIPSLLFNGVDFSGL

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=417, Percent_Identity=26.1390887290168, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789640, Length=219, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48305; Mature: 48305

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNENKKLAQWAMEYALKNGCQSSRVTLYNGSSSSFEIRDMKIDRLQQASENSMVVHLF
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHHCCCCCEEEEEE
VDGRYGSYSTNRLDKKELEKFIKDGISATRFLAEDKARTLPDASLYYKGGAADLRLVDPN
EECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCC
FDSVQPDDKVALAMSVCEEIMGKDDRIISANASYSDEKDFKYMVASNGFEGEASGSSYGL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCE
VGSVSIRGEGDARPESYWYDSSLYYDELVKTGIGTKALERVLRKLGQRKVASGKYTMIVD
EEEEEECCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
NMNSSRLLSPVIGAIYGSSIQQKNSFLLDKIDQKVASDKMTLIDEPHLVKASGARYFDSE
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCEEECCC
GVATKRLPVFEQGILKTYYIDTYSANKMGMEQTIGSPSILTMRNGDKDLDGLIASIDKGI
CCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHCCCCE
LVTGFNGGNCNSTTGDFSYGVEGFLIERGKLSQPISEMNATGNMLSLWSNLAEVGNDPRL
EEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCC
SSSWRIPSLLFNGVDFSGL
CCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MITNENKKLAQWAMEYALKNGCQSSRVTLYNGSSSSFEIRDMKIDRLQQASENSMVVHLF
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHHHHHHHCCCCCEEEEEE
VDGRYGSYSTNRLDKKELEKFIKDGISATRFLAEDKARTLPDASLYYKGGAADLRLVDPN
EECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCC
FDSVQPDDKVALAMSVCEEIMGKDDRIISANASYSDEKDFKYMVASNGFEGEASGSSYGL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCE
VGSVSIRGEGDARPESYWYDSSLYYDELVKTGIGTKALERVLRKLGQRKVASGKYTMIVD
EEEEEECCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
NMNSSRLLSPVIGAIYGSSIQQKNSFLLDKIDQKVASDKMTLIDEPHLVKASGARYFDSE
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCEEECCC
GVATKRLPVFEQGILKTYYIDTYSANKMGMEQTIGSPSILTMRNGDKDLDGLIASIDKGI
CCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHCCCCE
LVTGFNGGNCNSTTGDFSYGVEGFLIERGKLSQPISEMNATGNMLSLWSNLAEVGNDPRL
EEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCC
SSSWRIPSLLFNGVDFSGL
CCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]