Click here to switch to the map view.

The map label for this gene is pyrI

Identifier: 150007489

GI number: 150007489

Start: 987980

End: 988444

Strand: Reverse

Name: pyrI

Synonym: BDI_0840

Alternate gene names: 150007489

Gene position: 988444-987980 (Counterclockwise)

Preceding gene: 150007490

Following gene: 150007488

Centisome position: 20.54

GC content: 41.72

Gene sequence:

>465_bases
ATGTCAGCAGAAAAGAAAAAAGAGTTGCAGGTAGCCGCTTTAGAGAACGGTACCGCCATAGACCATATTCCTCCCAGCCA
ATTATTCAAGGTAGCCTCTTTACTGGGACTGGAGAAAATGAACAATACGATCACGATCGGCAATAATTTCCATAGCAACA
AGATGGGATGCAAGGGTATGATCAAGATCGCCGACAAATTTTTCGAGGAGGACGAGATCAACCGTATCGCCATGATCGCC
CCGAATATTATATTGAACATTATCCGTGATTATGAGGTTGTAGAGAAGAAGACGGTTACTTTACCCGACGAGCTGATTGG
CTTGGTCAAATGTAACAATCCGAAATGTATCACCAATAATGAGCCGATGCTTTCCCGCTTCCACGTGATCGACAAGGAGA
AGGGAACCATTAAATGTCATTATTGCGAACGTAAGATTAATAAAGAAGACATTATCATTAAATAA

Upstream 100 bases:

>100_bases
GGCTTATTACATCCAGCAGGCACGCAACGGGCTGTTCGCACGCCAAGCTATCATTTGCGAAGTTTTAGGTATATCTATCG
ACGAATAAAAACGAAGTTTT

Downstream 100 bases:

>100_bases
TTGAAAGTTGAGAATTGAGAATTGAGAATTAGCTTTACGGAATCTTTAATTCTCAATTCTCAACTTTCAATTATAAAAAC
GCAGTTTTTATTATGAAGCA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSAEKKKELQVAALENGTAIDHIPPSQLFKVASLLGLEKMNNTITIGNNFHSNKMGCKGMIKIADKFFEEDEINRIAMIA
PNIILNIIRDYEVVEKKTVTLPDELIGLVKCNNPKCITNNEPMLSRFHVIDKEKGTIKCHYCERKINKEDIIIK

Sequences:

>Translated_154_residues
MSAEKKKELQVAALENGTAIDHIPPSQLFKVASLLGLEKMNNTITIGNNFHSNKMGCKGMIKIADKFFEEDEINRIAMIA
PNIILNIIRDYEVVEKKTVTLPDELIGLVKCNNPKCITNNEPMLSRFHVIDKEKGTIKCHYCERKINKEDIIIK
>Mature_153_residues
SAEKKKELQVAALENGTAIDHIPPSQLFKVASLLGLEKMNNTITIGNNFHSNKMGCKGMIKIADKFFEEDEINRIAMIAP
NIILNIIRDYEVVEKKTVTLPDELIGLVKCNNPKCITNNEPMLSRFHVIDKEKGTIKCHYCERKINKEDIIIK

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=141, Percent_Identity=40.4255319148936, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_PARD8 (A6LA96)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302232.1
- ProteinModelPortal:   A6LA96
- SMR:   A6LA96
- STRING:   A6LA96
- GeneID:   5305994
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0840
- eggNOG:   COG1781
- HOGENOM:   HBG305327
- OMA:   TATINIV
- ProtClustDB:   PRK00893
- BioCyc:   PDIS435591:BDI_0840-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17468; Mature: 17337

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEKKKELQVAALENGTAIDHIPPSQLFKVASLLGLEKMNNTITIGNNFHSNKMGCKGM
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHH
IKIADKFFEEDEINRIAMIAPNIILNIIRDYEVVEKKTVTLPDELIGLVKCNNPKCITNN
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCEEEECC
EPMLSRFHVIDKEKGTIKCHYCERKINKEDIIIK
CCHHHHEEEEECCCCEEEEEEECCCCCCCCCEEC
>Mature Secondary Structure 
SAEKKKELQVAALENGTAIDHIPPSQLFKVASLLGLEKMNNTITIGNNFHSNKMGCKGM
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHH
IKIADKFFEEDEINRIAMIAPNIILNIIRDYEVVEKKTVTLPDELIGLVKCNNPKCITNN
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCEEEECC
EPMLSRFHVIDKEKGTIKCHYCERKINKEDIIIK
CCHHHHEEEEECCCCEEEEEEECCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA