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The map label for this gene is pyrB

Identifier: 150007490

GI number: 150007490

Start: 988457

End: 989377

Strand: Reverse

Name: pyrB

Synonym: BDI_0841

Alternate gene names: 150007490

Gene position: 989377-988457 (Counterclockwise)

Preceding gene: 150007491

Following gene: 150007489

Centisome position: 20.56

GC content: 49.29

Gene sequence:

>921_bases
ATGAAGAGTAAAAGTTTAGTTTCTATCGATCAATGTTCCAAAGAGGACATTCTTCGTATTCTGGATAACGCAAGGAAGTT
TGAGGAGAATCCGAACCGGAAATTACTGGAAGGCAAAGTCGCCGCCACCCTGTTCTTCGAGCCCTCTACACGTACGAGAC
TGAGTTTCGAGACAGCTGTAAACCGTTTAGGTGGACGGGTGATCGGTTTCTCGGACGCTTCCACGACTAGTTCCTCCAAG
GGCGAGACCTTGAAAGATACGATCTTGATGGTAAGCAATTACGTGGACTTGATCATTATGAGACATCATCTGGAAGGAGC
CGCCCGCTATGCTTCTGAGGTGACGGATGTCCCTATCATCAACGCTGGTGACGGGGCGAACCAACATCCCTCGCAAACCA
TGCTGGACCTGTATTCCATTCGCAAGACACAAGGCAAGCTGGATAACCTCACCATCACGATGGTCGGCGATTTGAAGTAT
GGCCGTACGGTTCACTCCTTGATCGTGGGAATGTCTCACTTCCATCCGACCTTCCACTTCGTCGCGCCGAACGAGCTGCG
CATGCCGGACGAGCAAAAGAACTTCTGCGACAAGCACGGGCTCAAATACGAAGAGCATACCGATTTCACGGAAGATATCA
TCAACCAGACGGATATCCTTTATATGACCCGTGTACAACGTGAGCGTTTCACAGACTTGGAAGAGTACGAGCGGGTAAAG
AACGTGTATATCCTGCGTAACGACATGCTGAGAAACAGCCGTGAAAACCTGCGTATCTTACATCCGCTACCCCGTGTGAA
CGAGATCGCTTACGATGTAGATGACAATCCAAAGGCTTATTACATCCAGCAGGCACGCAACGGGCTGTTCGCACGCCAAG
CTATCATTTGCGAAGTTTTAGGTATATCTATCGACGAATAA

Upstream 100 bases:

>100_bases
ATTATATCTCTGAATCAATAGCTGATTTTTTACAAATATTTTACATCAGTTATTAATTAGAAAGATAAGAAACAGACAAT
AAACATATTACAATATATAA

Downstream 100 bases:

>100_bases
AAACGAAGTTTTATGTCAGCAGAAAAGAAAAAAGAGTTGCAGGTAGCCGCTTTAGAGAACGGTACCGCCATAGACCATAT
TCCTCCCAGCCAATTATTCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKSKSLVSIDQCSKEDILRILDNARKFEENPNRKLLEGKVAATLFFEPSTRTRLSFETAVNRLGGRVIGFSDASTTSSSK
GETLKDTILMVSNYVDLIIMRHHLEGAARYASEVTDVPIINAGDGANQHPSQTMLDLYSIRKTQGKLDNLTITMVGDLKY
GRTVHSLIVGMSHFHPTFHFVAPNELRMPDEQKNFCDKHGLKYEEHTDFTEDIINQTDILYMTRVQRERFTDLEEYERVK
NVYILRNDMLRNSRENLRILHPLPRVNEIAYDVDDNPKAYYIQQARNGLFARQAIICEVLGISIDE

Sequences:

>Translated_306_residues
MKSKSLVSIDQCSKEDILRILDNARKFEENPNRKLLEGKVAATLFFEPSTRTRLSFETAVNRLGGRVIGFSDASTTSSSK
GETLKDTILMVSNYVDLIIMRHHLEGAARYASEVTDVPIINAGDGANQHPSQTMLDLYSIRKTQGKLDNLTITMVGDLKY
GRTVHSLIVGMSHFHPTFHFVAPNELRMPDEQKNFCDKHGLKYEEHTDFTEDIINQTDILYMTRVQRERFTDLEEYERVK
NVYILRNDMLRNSRENLRILHPLPRVNEIAYDVDDNPKAYYIQQARNGLFARQAIICEVLGISIDE
>Mature_306_residues
MKSKSLVSIDQCSKEDILRILDNARKFEENPNRKLLEGKVAATLFFEPSTRTRLSFETAVNRLGGRVIGFSDASTTSSSK
GETLKDTILMVSNYVDLIIMRHHLEGAARYASEVTDVPIINAGDGANQHPSQTMLDLYSIRKTQGKLDNLTITMVGDLKY
GRTVHSLIVGMSHFHPTFHFVAPNELRMPDEQKNFCDKHGLKYEEHTDFTEDIINQTDILYMTRVQRERFTDLEEYERVK
NVYILRNDMLRNSRENLRILHPLPRVNEIAYDVDDNPKAYYIQQARNGLFARQAIICEVLGISIDE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=40.327868852459, Blast_Score=257, Evalue=8e-69,
Organism=Escherichia coli, GI2367364, Length=300, Percent_Identity=53.3333333333333, Blast_Score=328, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=39.2523364485981, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=42.2077922077922, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=41.5032679738562, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PARD8 (A6LA97)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302233.1
- ProteinModelPortal:   A6LA97
- SMR:   A6LA97
- STRING:   A6LA97
- GeneID:   5305995
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0841
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   PDIS435591:BDI_0841-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35101; Mature: 35101

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKSLVSIDQCSKEDILRILDNARKFEENPNRKLLEGKVAATLFFEPSTRTRLSFETAV
CCCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHH
NRLGGRVIGFSDASTTSSSKGETLKDTILMVSNYVDLIIMRHHLEGAARYASEVTDVPII
HHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIRKTQGKLDNLTITMVGDLKYGRTVHSLIVGMSHFHPTFHF
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
VAPNELRMPDEQKNFCDKHGLKYEEHTDFTEDIINQTDILYMTRVQRERFTDLEEYERVK
ECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
NVYILRNDMLRNSRENLRILHPLPRVNEIAYDVDDNPKAYYIQQARNGLFARQAIICEVL
HEEEEEHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCEEEEEHHHCCHHHHHHHHHHHH
GISIDE
CCCCCC
>Mature Secondary Structure
MKSKSLVSIDQCSKEDILRILDNARKFEENPNRKLLEGKVAATLFFEPSTRTRLSFETAV
CCCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHH
NRLGGRVIGFSDASTTSSSKGETLKDTILMVSNYVDLIIMRHHLEGAARYASEVTDVPII
HHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIRKTQGKLDNLTITMVGDLKYGRTVHSLIVGMSHFHPTFHF
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
VAPNELRMPDEQKNFCDKHGLKYEEHTDFTEDIINQTDILYMTRVQRERFTDLEEYERVK
ECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
NVYILRNDMLRNSRENLRILHPLPRVNEIAYDVDDNPKAYYIQQARNGLFARQAIICEVL
HEEEEEHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCEEEEEHHHCCHHHHHHHHHHHH
GISIDE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA