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The map label for this gene is lplA [H]

Identifier: 150007339

GI number: 150007339

Start: 819526

End: 820269

Strand: Reverse

Name: lplA [H]

Synonym: BDI_0689

Alternate gene names: 150007339

Gene position: 820269-819526 (Counterclockwise)

Preceding gene: 150007340

Following gene: 150007338

Centisome position: 17.05

GC content: 42.88

Gene sequence:

>744_bases
ATGCTTTGCATTCACAATACACAAACGGATCCATACTTCAATTTGGCCGCCGAAGAGTATCTGCTCAAGAATTTCAAAGA
GAATATTTTCATGCTTTGGCAGAATGAACCATCAATCGTGATCGGTAAGCATCAGGATGTATGGGCCGAGGTAAATCTCA
AATTCGTACAAGACCAACAAATCAAGATAGCACGCAGGTTTTCCGGTGGTGGTGCCGTTTACCATGATCCGGGAAACCTG
AATCTTACATTTATAGAGACCGGCCAGATTATGCAGACCGACAAATATACCATCCAGATCATGAATTTTCTCAAGACATT
GGGAGTACATGTCGAGGTGGATGAACGGAAAGGATTAACTATCGGAGGATTTAAAATATCGGGTAGCGCACAGAGTATCC
ATAAGAATCGGTATATGCACCATGCGACCTTATTGTTTTCCACCGATCTGGATAGGCTTGTCACCTCCTTGAAAAGTACA
TCCCGTCAAGCACCAGAGAAAACAGGAGGTAAACGATCGACATTCGTTAAATCTGTACGGAGTCCGGTCACGAATATCAG
CGCCCATCTTCCGGCTTCTATCTCTATCCAGCATTTTAAGAAAGCGCTTCTTTACTATTTCACGATCAATAATCCCGGCA
TACAGGATTATTCCTTCACACAAGCAGACTTGGAAGCCATCTATCGGCTTAAAGAGCAGAAATATGCTACAGCCAGCTGG
AACTTTAACACTTATACACTATAA

Upstream 100 bases:

>100_bases
GATGAATTTAAGAAAAATATTTTCCCGCATCCGACAGTCAGTGAGATCCTCCACGAAAGTTTATATATCTAATAAAACGC
CTAACTAAAACAGTTTCCTG

Downstream 100 bases:

>100_bases
TACAGAAACTTACCTTCTTGATCTTTGTTCTTATTATATACCGTTTGTAGAGACGGGGCACGCCCCGTCTCCCACTATAT
AACAGTAATTAAAAAATCAA

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLCIHNTQTDPYFNLAAEEYLLKNFKENIFMLWQNEPSIVIGKHQDVWAEVNLKFVQDQQIKIARRFSGGGAVYHDPGNL
NLTFIETGQIMQTDKYTIQIMNFLKTLGVHVEVDERKGLTIGGFKISGSAQSIHKNRYMHHATLLFSTDLDRLVTSLKST
SRQAPEKTGGKRSTFVKSVRSPVTNISAHLPASISIQHFKKALLYYFTINNPGIQDYSFTQADLEAIYRLKEQKYATASW
NFNTYTL

Sequences:

>Translated_247_residues
MLCIHNTQTDPYFNLAAEEYLLKNFKENIFMLWQNEPSIVIGKHQDVWAEVNLKFVQDQQIKIARRFSGGGAVYHDPGNL
NLTFIETGQIMQTDKYTIQIMNFLKTLGVHVEVDERKGLTIGGFKISGSAQSIHKNRYMHHATLLFSTDLDRLVTSLKST
SRQAPEKTGGKRSTFVKSVRSPVTNISAHLPASISIQHFKKALLYYFTINNPGIQDYSFTQADLEAIYRLKEQKYATASW
NFNTYTL
>Mature_247_residues
MLCIHNTQTDPYFNLAAEEYLLKNFKENIFMLWQNEPSIVIGKHQDVWAEVNLKFVQDQQIKIARRFSGGGAVYHDPGNL
NLTFIETGQIMQTDKYTIQIMNFLKTLGVHVEVDERKGLTIGGFKISGSAQSIHKNRYMHHATLLFSTDLDRLVTSLKST
SRQAPEKTGGKRSTFVKSVRSPVTNISAHLPASISIQHFKKALLYYFTINNPGIQDYSFTQADLEAIYRLKEQKYATASW
NFNTYTL

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=192, Percent_Identity=39.0625, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI21729884, Length=192, Percent_Identity=39.0625, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI21729882, Length=192, Percent_Identity=39.0625, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI21729880, Length=192, Percent_Identity=39.0625, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI21729878, Length=192, Percent_Identity=39.0625, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1790846, Length=189, Percent_Identity=37.037037037037, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71981909, Length=211, Percent_Identity=33.6492890995261, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=199, Percent_Identity=34.1708542713568, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28573464, Length=205, Percent_Identity=33.6585365853659, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 28220; Mature: 28220

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCIHNTQTDPYFNLAAEEYLLKNFKENIFMLWQNEPSIVIGKHQDVWAEVNLKFVQDQQ
CEEEECCCCCCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEEEEEECCCC
IKIARRFSGGGAVYHDPGNLNLTFIETGQIMQTDKYTIQIMNFLKTLGVHVEVDERKGLT
EEEEEEECCCCEEEECCCCEEEEEEECCCEEECCHHHHHHHHHHHHHCCEEEECCCCCEE
IGGFKISGSAQSIHKNRYMHHATLLFSTDLDRLVTSLKSTSRQAPEKTGGKRSTFVKSVR
EECEEECCCHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHH
SPVTNISAHLPASISIQHFKKALLYYFTINNPGIQDYSFTQADLEAIYRLKEQKYATASW
CCHHHHHHCCCCCEEHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEEC
NFNTYTL
CCEEEEC
>Mature Secondary Structure
MLCIHNTQTDPYFNLAAEEYLLKNFKENIFMLWQNEPSIVIGKHQDVWAEVNLKFVQDQQ
CEEEECCCCCCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEEEEEECCCC
IKIARRFSGGGAVYHDPGNLNLTFIETGQIMQTDKYTIQIMNFLKTLGVHVEVDERKGLT
EEEEEEECCCCEEEECCCCEEEEEEECCCEEECCHHHHHHHHHHHHHCCEEEECCCCCEE
IGGFKISGSAQSIHKNRYMHHATLLFSTDLDRLVTSLKSTSRQAPEKTGGKRSTFVKSVR
EECEEECCCHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHH
SPVTNISAHLPASISIQHFKKALLYYFTINNPGIQDYSFTQADLEAIYRLKEQKYATASW
CCHHHHHHCCCCCEEHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEEC
NFNTYTL
CCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]