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The map label for this gene is asnB [H]

Identifier: 150006988

GI number: 150006988

Start: 365129

End: 366799

Strand: Reverse

Name: asnB [H]

Synonym: BDI_0324

Alternate gene names: 150006988

Gene position: 366799-365129 (Counterclockwise)

Preceding gene: 150006989

Following gene: 150006987

Centisome position: 7.62

GC content: 50.45

Gene sequence:

>1671_bases
ATGATCATGTGTGGTATAACAGCGATATTCAATATACACGACGGAGCGCAAGCCCTGCGTAAACAAGCGTTATTAATGAG
TAAACGAATCCGTCACCGGGGGCCGGACTGGAGTGGTATCTACCAAGGCAAAACCGCTATCTTAGCCCATGAACGCCTTT
CCATCGTAGACCCAGCCTCCGGAGGGCAGCCTTTGGTCAGCCCCGACGGGAAACTCATCCTCTGCGTCAACGGAGAGATC
TACAACCATCAGGAATTACGAGAACGGTTAAAAGGCGATTACGAGTTCCAAACCGGCTCCGATTGCGAGGTGATCCTCGC
CTTATACAAGAAGAAAGGGATTGATTTCATCGAGGATCTCAATGGTATTTTCGCCTTCGCTCTCTATGACGAGGAGAAAG
GGGTTTACTTGATCGCCCGTGACCCGATCGGCGTGATCCCGCTCTACATCGGATACGATGACGAGGGACACTTGATGGTA
TCTTCTGAGTTAAAAGGACTTGAAGGCTTCGCCACTCATTACGAGCCGTTCCTCCCCGGTCATTATTTCTATAGCGAAGA
CCGTAAACTCACGAGATGGTACACCCGTGACTGGATGGATTACGCCAACGTGAAAGATAATCCTACCGACGTACAACAAC
TTCACGATGCGCTGGAAGCCGCCGTCAAACGCCAACTGATGAGCGATGTCCCTTACGGCGTCCTTCTATCCGGAGGTTTG
GATTCCTCCATCACCTCTGCTATCGCCAAGAAATACGCGGCGAAACGCATCGAGACCAATGGGAAAGCGGACGCATGGTG
GCCTCAACTCCACTCGTTCGCCATCGGATTGAAAGATGCCCCGGACTTGATCGCCGCCCGTAAAGTAGCGGATGCGATCG
GTACGGTTCATCATGAGATCCATTATACGATACAAGAAGGATTGGACGCTTTACGAGATGTCATTTATCATATCGAGACC
TATGATGTCACTACCGTCCGAGCCTCTACCCCCATGTATTTATTGGCCCGTGTGATCCGGAGCATGGGTATCAAGATGGT
ACTAAGCGGCGAGGGTGCGGACGAGGTATTCGGGGGATATTTATATTTCCATAAGGCCCCGAATGCACAGGCTTTCCATG
AAGAGACCCTGCGTAAATTAAGCAAATTGTATTTATATGATTGCTTGCGTGCGAACAAAAGCCTTTGCGCATGGGGCGTA
GAAGGACGAGTGCCGTTCTTGGATAAAGAGTTTCTTGATGTGGCCATGCGTCTGAATCCGGTGGCAAAGATGTGCCCGGG
TACGATCATCGAGAAGAAGATACTTCGAGAAGCATTTTCCGACAGCTTGCCCAAGGAGATCGCTTGGCGGCAAAAAGAGC
AATTCTCGGATGGGGTAGGCTATGGCTGGATCGATACATTAAAGAAAATAACCTCCGAGTCGGTCACTGATGAAGAGATG
GCGAATGCCGCCAAACGATTCCCGATCAATCCGCCCCAAAACAAGGAAGAGTATTATTACCGCTCCATCTTTGAAGAGCA
TTTCCCCAGCGAAAGCGCAGCGAGAAGCGTACCTTCCATTCCTAGCGTAGCGTGTTCTACCGCAGAGGCTTTAGCTTGGG
ACTCGGCTTTCAAAAACATGAACGAGCCAAGCGGCAGGGCCATAAAAGGCGTACATGAATCCGCTTATTGA

Upstream 100 bases:

>100_bases
GAGCGAGCTTAGTCGTAAAAGCGATGGCCTCCGGCCGTAATGTCGCCCGATCCATCGACCGTTTCTTACAAACCAACTAA
TTTTCAACTTTCAATTTTTA

Downstream 100 bases:

>100_bases
CGAATATTTCTTTTCTTTAGTAGCAAAATGCACCAACTTTTATGAATAATAAACGCTGGTGCATTTAATTTTTATAATAA
CTTTGCCAGCTAGAGTCATA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MIMCGITAIFNIHDGAQALRKQALLMSKRIRHRGPDWSGIYQGKTAILAHERLSIVDPASGGQPLVSPDGKLILCVNGEI
YNHQELRERLKGDYEFQTGSDCEVILALYKKKGIDFIEDLNGIFAFALYDEEKGVYLIARDPIGVIPLYIGYDDEGHLMV
SSELKGLEGFATHYEPFLPGHYFYSEDRKLTRWYTRDWMDYANVKDNPTDVQQLHDALEAAVKRQLMSDVPYGVLLSGGL
DSSITSAIAKKYAAKRIETNGKADAWWPQLHSFAIGLKDAPDLIAARKVADAIGTVHHEIHYTIQEGLDALRDVIYHIET
YDVTTVRASTPMYLLARVIRSMGIKMVLSGEGADEVFGGYLYFHKAPNAQAFHEETLRKLSKLYLYDCLRANKSLCAWGV
EGRVPFLDKEFLDVAMRLNPVAKMCPGTIIEKKILREAFSDSLPKEIAWRQKEQFSDGVGYGWIDTLKKITSESVTDEEM
ANAAKRFPINPPQNKEEYYYRSIFEEHFPSESAARSVPSIPSVACSTAEALAWDSAFKNMNEPSGRAIKGVHESAY

Sequences:

>Translated_556_residues
MIMCGITAIFNIHDGAQALRKQALLMSKRIRHRGPDWSGIYQGKTAILAHERLSIVDPASGGQPLVSPDGKLILCVNGEI
YNHQELRERLKGDYEFQTGSDCEVILALYKKKGIDFIEDLNGIFAFALYDEEKGVYLIARDPIGVIPLYIGYDDEGHLMV
SSELKGLEGFATHYEPFLPGHYFYSEDRKLTRWYTRDWMDYANVKDNPTDVQQLHDALEAAVKRQLMSDVPYGVLLSGGL
DSSITSAIAKKYAAKRIETNGKADAWWPQLHSFAIGLKDAPDLIAARKVADAIGTVHHEIHYTIQEGLDALRDVIYHIET
YDVTTVRASTPMYLLARVIRSMGIKMVLSGEGADEVFGGYLYFHKAPNAQAFHEETLRKLSKLYLYDCLRANKSLCAWGV
EGRVPFLDKEFLDVAMRLNPVAKMCPGTIIEKKILREAFSDSLPKEIAWRQKEQFSDGVGYGWIDTLKKITSESVTDEEM
ANAAKRFPINPPQNKEEYYYRSIFEEHFPSESAARSVPSIPSVACSTAEALAWDSAFKNMNEPSGRAIKGVHESAY
>Mature_556_residues
MIMCGITAIFNIHDGAQALRKQALLMSKRIRHRGPDWSGIYQGKTAILAHERLSIVDPASGGQPLVSPDGKLILCVNGEI
YNHQELRERLKGDYEFQTGSDCEVILALYKKKGIDFIEDLNGIFAFALYDEEKGVYLIARDPIGVIPLYIGYDDEGHLMV
SSELKGLEGFATHYEPFLPGHYFYSEDRKLTRWYTRDWMDYANVKDNPTDVQQLHDALEAAVKRQLMSDVPYGVLLSGGL
DSSITSAIAKKYAAKRIETNGKADAWWPQLHSFAIGLKDAPDLIAARKVADAIGTVHHEIHYTIQEGLDALRDVIYHIET
YDVTTVRASTPMYLLARVIRSMGIKMVLSGEGADEVFGGYLYFHKAPNAQAFHEETLRKLSKLYLYDCLRANKSLCAWGV
EGRVPFLDKEFLDVAMRLNPVAKMCPGTIIEKKILREAFSDSLPKEIAWRQKEQFSDGVGYGWIDTLKKITSESVTDEEM
ANAAKRFPINPPQNKEEYYYRSIFEEHFPSESAARSVPSIPSVACSTAEALAWDSAFKNMNEPSGRAIKGVHESAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=545, Percent_Identity=41.8348623853211, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI168229252, Length=545, Percent_Identity=41.8348623853211, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI168229250, Length=545, Percent_Identity=41.8348623853211, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI296010848, Length=519, Percent_Identity=42.3892100192678, Blast_Score=355, Evalue=8e-98,
Organism=Homo sapiens, GI296010852, Length=451, Percent_Identity=43.2372505543237, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI296010850, Length=451, Percent_Identity=43.2372505543237, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=64.0794223826715, Blast_Score=748, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=571, Percent_Identity=46.0595446584939, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71993933, Length=533, Percent_Identity=47.6547842401501, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17560178, Length=525, Percent_Identity=37.1428571428571, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=349, Percent_Identity=39.541547277937, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321563, Length=582, Percent_Identity=49.3127147766323, Blast_Score=525, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325403, Length=583, Percent_Identity=48.885077186964, Blast_Score=521, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=581, Percent_Identity=46.4716006884682, Blast_Score=476, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62489; Mature: 62489

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMCGITAIFNIHDGAQALRKQALLMSKRIRHRGPDWSGIYQGKTAILAHERLSIVDPAS
CEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEECCCCCEECCCC
GGQPLVSPDGKLILCVNGEIYNHQELRERLKGDYEFQTGSDCEVILALYKKKGIDFIEDL
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHC
NGIFAFALYDEEKGVYLIARDPIGVIPLYIGYDDEGHLMVSSELKGLEGFATHYEPFLPG
CCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHCCCCCCC
HYFYSEDRKLTRWYTRDWMDYANVKDNPTDVQQLHDALEAAVKRQLMSDVPYGVLLSGGL
CEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
DSSITSAIAKKYAAKRIETNGKADAWWPQLHSFAIGLKDAPDLIAARKVADAIGTVHHEI
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HYTIQEGLDALRDVIYHIETYDVTTVRASTPMYLLARVIRSMGIKMVLSGEGADEVFGGY
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCE
LYFHKAPNAQAFHEETLRKLSKLYLYDCLRANKSLCAWGVEGRVPFLDKEFLDVAMRLNP
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCH
VAKMCPGTIIEKKILREAFSDSLPKEIAWRQKEQFSDGVGYGWIDTLKKITSESVTDEEM
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHH
ANAAKRFPINPPQNKEEYYYRSIFEEHFPSESAARSVPSIPSVACSTAEALAWDSAFKNM
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
NEPSGRAIKGVHESAY
CCCCCCHHHCHHHCCC
>Mature Secondary Structure
MIMCGITAIFNIHDGAQALRKQALLMSKRIRHRGPDWSGIYQGKTAILAHERLSIVDPAS
CEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEECCCCCEECCCC
GGQPLVSPDGKLILCVNGEIYNHQELRERLKGDYEFQTGSDCEVILALYKKKGIDFIEDL
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHC
NGIFAFALYDEEKGVYLIARDPIGVIPLYIGYDDEGHLMVSSELKGLEGFATHYEPFLPG
CCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHCCCCCCC
HYFYSEDRKLTRWYTRDWMDYANVKDNPTDVQQLHDALEAAVKRQLMSDVPYGVLLSGGL
CEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
DSSITSAIAKKYAAKRIETNGKADAWWPQLHSFAIGLKDAPDLIAARKVADAIGTVHHEI
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HYTIQEGLDALRDVIYHIETYDVTTVRASTPMYLLARVIRSMGIKMVLSGEGADEVFGGY
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCE
LYFHKAPNAQAFHEETLRKLSKLYLYDCLRANKSLCAWGVEGRVPFLDKEFLDVAMRLNP
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCH
VAKMCPGTIIEKKILREAFSDSLPKEIAWRQKEQFSDGVGYGWIDTLKKITSESVTDEEM
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHH
ANAAKRFPINPPQNKEEYYYRSIFEEHFPSESAARSVPSIPSVACSTAEALAWDSAFKNM
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
NEPSGRAIKGVHESAY
CCCCCCHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]