Click here to switch to the map view.

The map label for this gene is gltB [H]

Identifier: 150006989

GI number: 150006989

Start: 366820

End: 368244

Strand: Reverse

Name: gltB [H]

Synonym: BDI_0325

Alternate gene names: 150006989

Gene position: 368244-366820 (Counterclockwise)

Preceding gene: 150006990

Following gene: 150006988

Centisome position: 7.65

GC content: 53.89

Gene sequence:

>1425_bases
ATGGGAAACCCAAGAGCATTTCTAACGATACATAGACAAGAGGCGGGCTATCGTCCCGTCCACGAACGTATAGATGATTT
CAGCGAGGTAGAACAGACCTTGAACAGCGGCGACCGCCGTACGCAGGCCTCCCGCTGCATGGATTGTGGCGTACCCTTCT
GCCACTGGGCCTGCCCGCTGGGTAACAAGCAACCCGAATGGCAAGATTTGTTATACAAAGGCCGTTACAAAGAGGCATAT
CACGTATTGGAGCAGACCGACGACTTCCCGGAGTTCACGGGACGTGTCTGCCCCGCCCTCTGCGAGAAAAGCTGCGTACT
GAAACTTAGCTGCGACGAACCTGTCACGATCCGTGAGAACGAGGCGGCTATCGTGGAAGCCGCTTTCCGGGAAGGATACG
TACAGCCTATCCAACCAGTACGCAACGGGAAAAAAGTAGCCGTGATCGGTAGCGGTCCCGCCGGATTGACCGTGGCGAAC
CAATTGAACCGGAAAGGATATGAAGTCACCGTATTCGAGAAAGATGAGTTACCGGGCGGCTTGCTACGTTTCGGCATCCC
CAATTTCAAGCTAGGCAAGCACATTATCGATCGCCGTATCCGCATCATGGAGCAAGAAGGGATTCTTTTCCGTGTAAACA
CGATGGTCGGCAAAGAGATATTAGCGAAAGATGTAGTGAAAGATTTCGACGCTGTTTGCGTAGCGATCGGCGCCGAGGTT
CCCCGGGATCTATCTATCGAGGGACGTCATTTAAGAGGCGTACATTTCGCCTTGGAGTTATTAAGCCAGCAGAACCGGAT
CTTAGAAGGCATCCCCATCCCGCCCAAAAGCCAGATCAATTGCAAAGGCAAGAAAGTCTTAGTGATTGGCGGAGGCGATA
CGGGTAGCGATTGCGTAGGGACAGCGAACCGTCATGGGGCGGCCTGTGTCACCCAGATCGAGATCATGCCCCAGCCACCC
GTCGGACAGAATCCGGCGACACCTTGGCCCATGTATCCCCAAGTACTGAAAACCAGCAGCAGCCATGAGGAAGGTTGTAT
CCGCCGGTGGAACCTAGCCTCTAACCGTTTTATCGGAGAGAAAAACAACCTGATAGGGGTTGAGGTAGAAGAAGTCGAAT
GGGTTCCCTCCCCAGACGGAGGCCGTCCGCAAATGAGACAGACCGGCAAGAAAGAGATCATCGAGGCCGATCTGGTTTTC
CTCGCCATGGGCTTCCTGCATCCGGTACAAGAAGGATTGATCAAAGAACTGCGATTGGCTACCGACAACCGCAATAACAT
CGCCGTAGACAACGCCGGATATACCGCCAACAGCAACCTATTCGCCTGCGGAGACGCTGTCAGCGGAGCGAGCTTAGTCG
TAAAAGCGATGGCCTCCGGCCGTAATGTCGCCCGATCCATCGACCGTTTCTTACAAACCAACTAA

Upstream 100 bases:

>100_bases
TACAAGAAAGTACTCCAAGAGGAACAGATGCGCAAACTCCAACGCAAGATTGCGGAGATGCAGAAAGACTACTAAATATT
TACACAAAAACAAGAGAACT

Downstream 100 bases:

>100_bases
TTTTCAACTTTCAATTTTTAATGATCATGTGTGGTATAACAGCGATATTCAATATACACGACGGAGCGCAAGCCCTGCGT
AAACAAGCGTTATTAATGAG

Product: glutamate synthase, small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MGNPRAFLTIHRQEAGYRPVHERIDDFSEVEQTLNSGDRRTQASRCMDCGVPFCHWACPLGNKQPEWQDLLYKGRYKEAY
HVLEQTDDFPEFTGRVCPALCEKSCVLKLSCDEPVTIRENEAAIVEAAFREGYVQPIQPVRNGKKVAVIGSGPAGLTVAN
QLNRKGYEVTVFEKDELPGGLLRFGIPNFKLGKHIIDRRIRIMEQEGILFRVNTMVGKEILAKDVVKDFDAVCVAIGAEV
PRDLSIEGRHLRGVHFALELLSQQNRILEGIPIPPKSQINCKGKKVLVIGGGDTGSDCVGTANRHGAACVTQIEIMPQPP
VGQNPATPWPMYPQVLKTSSSHEEGCIRRWNLASNRFIGEKNNLIGVEVEEVEWVPSPDGGRPQMRQTGKKEIIEADLVF
LAMGFLHPVQEGLIKELRLATDNRNNIAVDNAGYTANSNLFACGDAVSGASLVVKAMASGRNVARSIDRFLQTN

Sequences:

>Translated_474_residues
MGNPRAFLTIHRQEAGYRPVHERIDDFSEVEQTLNSGDRRTQASRCMDCGVPFCHWACPLGNKQPEWQDLLYKGRYKEAY
HVLEQTDDFPEFTGRVCPALCEKSCVLKLSCDEPVTIRENEAAIVEAAFREGYVQPIQPVRNGKKVAVIGSGPAGLTVAN
QLNRKGYEVTVFEKDELPGGLLRFGIPNFKLGKHIIDRRIRIMEQEGILFRVNTMVGKEILAKDVVKDFDAVCVAIGAEV
PRDLSIEGRHLRGVHFALELLSQQNRILEGIPIPPKSQINCKGKKVLVIGGGDTGSDCVGTANRHGAACVTQIEIMPQPP
VGQNPATPWPMYPQVLKTSSSHEEGCIRRWNLASNRFIGEKNNLIGVEVEEVEWVPSPDGGRPQMRQTGKKEIIEADLVF
LAMGFLHPVQEGLIKELRLATDNRNNIAVDNAGYTANSNLFACGDAVSGASLVVKAMASGRNVARSIDRFLQTN
>Mature_473_residues
GNPRAFLTIHRQEAGYRPVHERIDDFSEVEQTLNSGDRRTQASRCMDCGVPFCHWACPLGNKQPEWQDLLYKGRYKEAYH
VLEQTDDFPEFTGRVCPALCEKSCVLKLSCDEPVTIRENEAAIVEAAFREGYVQPIQPVRNGKKVAVIGSGPAGLTVANQ
LNRKGYEVTVFEKDELPGGLLRFGIPNFKLGKHIIDRRIRIMEQEGILFRVNTMVGKEILAKDVVKDFDAVCVAIGAEVP
RDLSIEGRHLRGVHFALELLSQQNRILEGIPIPPKSQINCKGKKVLVIGGGDTGSDCVGTANRHGAACVTQIEIMPQPPV
GQNPATPWPMYPQVLKTSSSHEEGCIRRWNLASNRFIGEKNNLIGVEVEEVEWVPSPDGGRPQMRQTGKKEIIEADLVFL
AMGFLHPVQEGLIKELRLATDNRNNIAVDNAGYTANSNLFACGDAVSGASLVVKAMASGRNVARSIDRFLQTN

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=486, Percent_Identity=24.0740740740741, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=35.2459016393443, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788811, Length=473, Percent_Identity=34.2494714587738, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082180, Length=474, Percent_Identity=32.7004219409283, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788468, Length=434, Percent_Identity=26.7281105990783, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1789243, Length=426, Percent_Identity=26.5258215962441, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=483, Percent_Identity=41.2008281573499, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71984108, Length=475, Percent_Identity=24, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=158, Percent_Identity=29.746835443038, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=40.8247422680412, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=44.1237113402062, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=44.1237113402062, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=44.4906444906445, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=44.4906444906445, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24640763, Length=311, Percent_Identity=26.0450160771704, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858217, Length=311, Percent_Identity=26.0450160771704, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52363; Mature: 52231

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNPRAFLTIHRQEAGYRPVHERIDDFSEVEQTLNSGDRRTQASRCMDCGVPFCHWACPL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEECCC
GNKQPEWQDLLYKGRYKEAYHVLEQTDDFPEFTGRVCPALCEKSCVLKLSCDEPVTIREN
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECC
EAAIVEAAFREGYVQPIQPVRNGKKVAVIGSGPAGLTVANQLNRKGYEVTVFEKDELPGG
CHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCC
LLRFGIPNFKLGKHIIDRRIRIMEQEGILFRVNTMVGKEILAKDVVKDFDAVCVAIGAEV
EEECCCCCCHHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PRDLSIEGRHLRGVHFALELLSQQNRILEGIPIPPKSQINCKGKKVLVIGGGDTGSDCVG
CCCCCCCCCEEHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
TANRHGAACVTQIEIMPQPPVGQNPATPWPMYPQVLKTSSSHEEGCIRRWNLASNRFIGE
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHEECCCCCCCEECC
KNNLIGVEVEEVEWVPSPDGGRPQMRQTGKKEIIEADLVFLAMGFLHPVQEGLIKELRLA
CCCEEEEEEHHEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDNRNNIAVDNAGYTANSNLFACGDAVSGASLVVKAMASGRNVARSIDRFLQTN
CCCCCCEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNPRAFLTIHRQEAGYRPVHERIDDFSEVEQTLNSGDRRTQASRCMDCGVPFCHWACPL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEECCC
GNKQPEWQDLLYKGRYKEAYHVLEQTDDFPEFTGRVCPALCEKSCVLKLSCDEPVTIREN
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECC
EAAIVEAAFREGYVQPIQPVRNGKKVAVIGSGPAGLTVANQLNRKGYEVTVFEKDELPGG
CHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCCCC
LLRFGIPNFKLGKHIIDRRIRIMEQEGILFRVNTMVGKEILAKDVVKDFDAVCVAIGAEV
EEECCCCCCHHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PRDLSIEGRHLRGVHFALELLSQQNRILEGIPIPPKSQINCKGKKVLVIGGGDTGSDCVG
CCCCCCCCCEEHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
TANRHGAACVTQIEIMPQPPVGQNPATPWPMYPQVLKTSSSHEEGCIRRWNLASNRFIGE
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHEECCCCCCCEECC
KNNLIGVEVEEVEWVPSPDGGRPQMRQTGKKEIIEADLVFLAMGFLHPVQEGLIKELRLA
CCCEEEEEEHHEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDNRNNIAVDNAGYTANSNLFACGDAVSGASLVVKAMASGRNVARSIDRFLQTN
CCCCCCEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]