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The map label for this gene is dapF

Identifier: 150006986

GI number: 150006986

Start: 361765

End: 362574

Strand: Reverse

Name: dapF

Synonym: BDI_0322

Alternate gene names: 150006986

Gene position: 362574-361765 (Counterclockwise)

Preceding gene: 150006987

Following gene: 150006985

Centisome position: 7.54

GC content: 52.47

Gene sequence:

>810_bases
ATGACAAAATCAATCAGATTCACAAAGATGCACGGTGCCGGAAATGATTACATCTATGTAAACACGATGGCCTATCCGAT
AGACAATCCCGAGGAATTATCTATCGCATGGAGCAAGCCGCACACAGGCATAGGATCTGACGGTTTGGTCTTGATAGGTT
CTTCAGACATCGCGGATTTCAGTATGCATATCTATAATGCGGACGGCTCCGAGGCGATGATGTGCGGAAACGCATCCAGA
TGCATTGGCAAATATGTCTATGAGGCCGGCCTAACGCAAAAGAACAAGGTTACCTTAGAGACCTTATCAGGTATCAAAGA
GCTACAATTGAAAATAGAGCGTGAAGAGGTCACCGAGGTCACGGTAGATATGGGAATGCCTGCCGTAGGCGAGATGGCCT
TGGAGGTTGAGGCTGGAGGAGAAATTTACACCGGCACCGTCATCTCTATGGGAAATCCTCACTTGGTTATCTTCGTGGAC
GAGATGGACCAGGTCGGTCTGGCAAGCGTAGGCCCGTTACTGGAATGCCTCCCGCTTTTTCCGGACCGGACAAACGTGGA
GTTCGTGCAGGTGCTAAAGCCGGACGAGGTACGGATGAGGGTGTGGGAACGAGGTTCCGGCATCACACAAGCCTGCGGAA
CCGGAGCTTGCGCCACCGCCGTGGCCGGGGTCGTACGTGGGAAGACAAAGCGAAAGACGAAGGTCATCATGGATGGGGGC
CCTTTAACCATCGAGTGGGACGAGCGGAGCGGTAAGGTCTTTATGACCGGCGGAGCGGTCAACGTGTTCGAGGGATTCAT
CAAAGTCTGA

Upstream 100 bases:

>100_bases
AAACATTCGAGCCTCTCACCTGTTATATACTTATCAAAACGTTATATATTTGCATCACAATAAAACAAAAAGAAATAACA
TAAAGAATAATATCATCAAA

Downstream 100 bases:

>100_bases
CATGGGGAACAAGACAAAACAACAAAAACAACAACATAAAAACAAAAGAACTGAGTATGGCACTAATTAATGAACATTTC
CTGAAACTACAGAACAATTA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTKSIRFTKMHGAGNDYIYVNTMAYPIDNPEELSIAWSKPHTGIGSDGLVLIGSSDIADFSMHIYNADGSEAMMCGNASR
CIGKYVYEAGLTQKNKVTLETLSGIKELQLKIEREEVTEVTVDMGMPAVGEMALEVEAGGEIYTGTVISMGNPHLVIFVD
EMDQVGLASVGPLLECLPLFPDRTNVEFVQVLKPDEVRMRVWERGSGITQACGTGACATAVAGVVRGKTKRKTKVIMDGG
PLTIEWDERSGKVFMTGGAVNVFEGFIKV

Sequences:

>Translated_269_residues
MTKSIRFTKMHGAGNDYIYVNTMAYPIDNPEELSIAWSKPHTGIGSDGLVLIGSSDIADFSMHIYNADGSEAMMCGNASR
CIGKYVYEAGLTQKNKVTLETLSGIKELQLKIEREEVTEVTVDMGMPAVGEMALEVEAGGEIYTGTVISMGNPHLVIFVD
EMDQVGLASVGPLLECLPLFPDRTNVEFVQVLKPDEVRMRVWERGSGITQACGTGACATAVAGVVRGKTKRKTKVIMDGG
PLTIEWDERSGKVFMTGGAVNVFEGFIKV
>Mature_268_residues
TKSIRFTKMHGAGNDYIYVNTMAYPIDNPEELSIAWSKPHTGIGSDGLVLIGSSDIADFSMHIYNADGSEAMMCGNASRC
IGKYVYEAGLTQKNKVTLETLSGIKELQLKIEREEVTEVTVDMGMPAVGEMALEVEAGGEIYTGTVISMGNPHLVIFVDE
MDQVGLASVGPLLECLPLFPDRTNVEFVQVLKPDEVRMRVWERGSGITQACGTGACATAVAGVVRGKTKRKTKVIMDGGP
LTIEWDERSGKVFMTGGAVNVFEGFIKV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=278, Percent_Identity=37.0503597122302, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PARD8 (A6L8U3)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001301729.1
- ProteinModelPortal:   A6L8U3
- SMR:   A6L8U3
- STRING:   A6L8U3
- GeneID:   5305477
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0322
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   PDIS435591:BDI_0322-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29083; Mature: 28952

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSIRFTKMHGAGNDYIYVNTMAYPIDNPEELSIAWSKPHTGIGSDGLVLIGSSDIADF
CCCCEEEEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEE
SMHIYNADGSEAMMCGNASRCIGKYVYEAGLTQKNKVTLETLSGIKELQLKIEREEVTEV
EEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEEEECHHHEEE
TVDMGMPAVGEMALEVEAGGEIYTGTVISMGNPHLVIFVDEMDQVGLASVGPLLECLPLF
EEECCCCCCCCEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
PDRTNVEFVQVLKPDEVRMRVWERGSGITQACGTGACATAVAGVVRGKTKRKTKVIMDGG
CCCCCCEEEEEECCCHHEEHEECCCCCCHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCC
PLTIEWDERSGKVFMTGGAVNVFEGFIKV
CEEEEECCCCCEEEEECCCHHHHHHHHCC
>Mature Secondary Structure 
TKSIRFTKMHGAGNDYIYVNTMAYPIDNPEELSIAWSKPHTGIGSDGLVLIGSSDIADF
CCCEEEEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEE
SMHIYNADGSEAMMCGNASRCIGKYVYEAGLTQKNKVTLETLSGIKELQLKIEREEVTEV
EEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEEEECHHHEEE
TVDMGMPAVGEMALEVEAGGEIYTGTVISMGNPHLVIFVDEMDQVGLASVGPLLECLPLF
EEECCCCCCCCEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
PDRTNVEFVQVLKPDEVRMRVWERGSGITQACGTGACATAVAGVVRGKTKRKTKVIMDGG
CCCCCCEEEEEECCCHHEEHEECCCCCCHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCC
PLTIEWDERSGKVFMTGGAVNVFEGFIKV
CEEEEECCCCCEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA