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The map label for this gene is ogt [H]

Identifier: 150006920

GI number: 150006920

Start: 282105

End: 282605

Strand: Reverse

Name: ogt [H]

Synonym: BDI_0256

Alternate gene names: 150006920

Gene position: 282605-282105 (Counterclockwise)

Preceding gene: 150006923

Following gene: 150006919

Centisome position: 5.87

GC content: 51.1

Gene sequence:

>501_bases
ATGCAATACACCGACAAATATCGATCTCCCTTGGGAGAAATAACCCTAGCCAGCGATGGCGACCACCTGACAGGATTATG
GTTCGACGGGCAAAAATATTTTGCCGCCACTTTATCGGCGGAACACCAAGAAAGAGAACTGCCGATATTCGAACAAGCCA
AAGAGTGGCTGGATAGCTATTTCATGGGTAAGATTCCCAGCTTCACCCCTCCTATCCATCTGGAGGATACGCCTTTCCGA
CTTGCGGTATGGGACTTATTGAGCAAAATACCTTATGGCGAAGTCGTAACCTACAAGGAGCTCGCCGGCGAAATCGCCCG
GCAAAAAGGTATCCCGAGCATGTCTACGCAAGCGATCGGAGGAGCGGTAGGCCATAACCCAATCAGTATCATCATCCCCT
GCCACCGTGTCGTAGGCAGCGATGGCAGCCTAACCGGATACGCAGGAGGTGTCGACAAAAAGAGAAGCCTGCTTACTATC
GAGCAGGCCAAAGGATTCTAA

Upstream 100 bases:

>100_bases
CAAATATAAATAAAAGTAGGATTCCTTATCTCATTTCCGCCCTTAGATATTGGCTACTTTCGAAAATTTTTCTAACTTAC
GCCCTTATTTGATAAACATC

Downstream 100 bases:

>100_bases
AACCGTATCAGTATGCGCACATTTCGTTCTATCGAAGAATTGGTTCGTACGCTCGACCGAGAGAGGGAACTGCTACGTGA
GATGTTTCATAAACGGACCT

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MQYTDKYRSPLGEITLASDGDHLTGLWFDGQKYFAATLSAEHQERELPIFEQAKEWLDSYFMGKIPSFTPPIHLEDTPFR
LAVWDLLSKIPYGEVVTYKELAGEIARQKGIPSMSTQAIGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGVDKKRSLLTI
EQAKGF

Sequences:

>Translated_166_residues
MQYTDKYRSPLGEITLASDGDHLTGLWFDGQKYFAATLSAEHQERELPIFEQAKEWLDSYFMGKIPSFTPPIHLEDTPFR
LAVWDLLSKIPYGEVVTYKELAGEIARQKGIPSMSTQAIGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGVDKKRSLLTI
EQAKGF
>Mature_166_residues
MQYTDKYRSPLGEITLASDGDHLTGLWFDGQKYFAATLSAEHQERELPIFEQAKEWLDSYFMGKIPSFTPPIHLEDTPFR
LAVWDLLSKIPYGEVVTYKELAGEIARQKGIPSMSTQAIGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGVDKKRSLLTI
EQAKGF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=117, Percent_Identity=35.042735042735, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=46.2264150943396, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788542, Length=142, Percent_Identity=35.9154929577465, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=93, Percent_Identity=43.010752688172, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=43.010752688172, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=99, Percent_Identity=38.3838383838384, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18330; Mature: 18330

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTDKYRSPLGEITLASDGDHLTGLWFDGQKYFAATLSAEHQERELPIFEQAKEWLDSY
CCCCHHHCCCCCCEEEECCCCEEEEEEECCCEEEEEECCCCHHHCCCCHHHHHHHHHHHH
FMGKIPSFTPPIHLEDTPFRLAVWDLLSKIPYGEVVTYKELAGEIARQKGIPSMSTQAIG
HHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHC
GAVGHNPISIIIPCHRVVGSDGSLTGYAGGVDKKRSLLTIEQAKGF
CCCCCCCEEEEEECHHHHCCCCCCCEECCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MQYTDKYRSPLGEITLASDGDHLTGLWFDGQKYFAATLSAEHQERELPIFEQAKEWLDSY
CCCCHHHCCCCCCEEEECCCCEEEEEEECCCEEEEEECCCCHHHCCCCHHHHHHHHHHHH
FMGKIPSFTPPIHLEDTPFRLAVWDLLSKIPYGEVVTYKELAGEIARQKGIPSMSTQAIG
HHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHC
GAVGHNPISIIIPCHRVVGSDGSLTGYAGGVDKKRSLLTIEQAKGF
CCCCCCCEEEEEECHHHHCCCCCCCEECCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]