Click here to switch to the map view.

The map label for this gene is amiA [H]

Identifier: 150006672

GI number: 150006672

Start: 5160725

End: 5161954

Strand: Direct

Name: amiA [H]

Synonym: BVU_4193

Alternate gene names: 150006672

Gene position: 5160725-5161954 (Clockwise)

Preceding gene: 150006670

Following gene: 150006673

Centisome position: 99.95

GC content: 39.11

Gene sequence:

>1230_bases
ATGAAAACCGATAGAATTAAAATAATATTCCTGTTGTGTAGCCTGTGGCTGGTCTTTCCCTGTTTCTGCCTGGCGGCAAA
GGGAAAGGACTTTGTAGTCGTCATTGATCCCGGTCATGGCGGACATGATCCCGGTGCTATCGGCAGACGTGGAAAAGAAA
AAAACATCAATCTGAACGTAGCTTTAAAAGTAGGACAACTGATCCAAAATAACTGTAATGACGTAAAGGTAATATATACC
CGGAAAACAGATGTCTTTATCCCTTTGGATCGTCGTGCACAAATAGCCAATAATGCCAAGGCCGATTTATTCATCTCTAT
ACATACTAATTCCATAGCCCGTGGCAGAACTGTACGGGGAGCAGAGACTTATACGTTAGGACTCCACCGTACTGAAGAAA
ATTTAGAAGTTGCTAAAAAAGAGAACTCGGTTATTTTGATAGAGGATAATTATGAACAACGTTATGCCGGATTCAATCCA
AATTCTTCTGAAAGCTATATTATTTTTGAATTTGTACAAGACAAAAATATGGAGAAGAGTGTAAAATTAGCCACTCTTAT
CCAAAAACAATTCAAGAATACAGCCAAACGAATAGACAAAGGAGTACATCAAGCCGGATTCCTAGTTCTACGTGCCACTT
CCATGCCTAGTGTTTTGGTTGAATTAGGCTATATCTCAACACCGGATGAAGAACAATATCTTTTATCCGATGCAGGCACT
ACGGCGTTATCTAACAGCATTTATAAAGCATTCCTCAATTACAAGCGCGAACATGACGCCCCCATCGGCCGCAGCAGAGT
ACAAGAACAAGAATTACCGGAACCAGAAAACAAACCGAAAGAATCAAGAATAGAAATACAAACTGCGGAACCGGACACTG
CAACGGAACCGGACAAGATTACCAAAAAGTCTGTGCCTGCCACACAAAAAAAGATCACAGGCGACCAAGCCCGAACATCT
GCCAAGCCTGTATTTAAAATACAAATATTGGTATCAAACAAAATATTGCCCAAAGGAAGCAAACAACTGAAAGGTGTATC
ACCTGTTAGCTATTACCGCGAAAAAGGACTTTATAAATATACTTACGGAGAAAATACGGATTATAATAAAATACTACGCA
TGAAACGTAATATTACTCCTAAATTTAAGGACGCTTTCATTATCGCTTTCAAAAACGGAGAGAAAATGAATGTGAACGAA
GCCATCAAAGAATTTAAAAAGAATAGATAA

Upstream 100 bases:

>100_bases
ATTTTTCGGGTTTTGTGGCAAGTTTCGAATAAAAAACGCAAAACTTAAAGACAAAAGCCGAGTTTTCACTAAAAATCGCT
ACATTTGCAACTTAATTGAT

Downstream 100 bases:

>100_bases
TATAAAAGGTATAATTGAAATGACGAAAGAGGTAAAAATAGGATTAATAGGCATCGCTGCATTGGCTATGCTGATCTTTG
GTATCAACTACCTGAAAGGA

Product: putative N-acetylmuramoyl-L-alanine amidase amiA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKTDRIKIIFLLCSLWLVFPCFCLAAKGKDFVVVIDPGHGGHDPGAIGRRGKEKNINLNVALKVGQLIQNNCNDVKVIYT
RKTDVFIPLDRRAQIANNAKADLFISIHTNSIARGRTVRGAETYTLGLHRTEENLEVAKKENSVILIEDNYEQRYAGFNP
NSSESYIIFEFVQDKNMEKSVKLATLIQKQFKNTAKRIDKGVHQAGFLVLRATSMPSVLVELGYISTPDEEQYLLSDAGT
TALSNSIYKAFLNYKREHDAPIGRSRVQEQELPEPENKPKESRIEIQTAEPDTATEPDKITKKSVPATQKKITGDQARTS
AKPVFKIQILVSNKILPKGSKQLKGVSPVSYYREKGLYKYTYGENTDYNKILRMKRNITPKFKDAFIIAFKNGEKMNVNE
AIKEFKKNR

Sequences:

>Translated_409_residues
MKTDRIKIIFLLCSLWLVFPCFCLAAKGKDFVVVIDPGHGGHDPGAIGRRGKEKNINLNVALKVGQLIQNNCNDVKVIYT
RKTDVFIPLDRRAQIANNAKADLFISIHTNSIARGRTVRGAETYTLGLHRTEENLEVAKKENSVILIEDNYEQRYAGFNP
NSSESYIIFEFVQDKNMEKSVKLATLIQKQFKNTAKRIDKGVHQAGFLVLRATSMPSVLVELGYISTPDEEQYLLSDAGT
TALSNSIYKAFLNYKREHDAPIGRSRVQEQELPEPENKPKESRIEIQTAEPDTATEPDKITKKSVPATQKKITGDQARTS
AKPVFKIQILVSNKILPKGSKQLKGVSPVSYYREKGLYKYTYGENTDYNKILRMKRNITPKFKDAFIIAFKNGEKMNVNE
AIKEFKKNR
>Mature_409_residues
MKTDRIKIIFLLCSLWLVFPCFCLAAKGKDFVVVIDPGHGGHDPGAIGRRGKEKNINLNVALKVGQLIQNNCNDVKVIYT
RKTDVFIPLDRRAQIANNAKADLFISIHTNSIARGRTVRGAETYTLGLHRTEENLEVAKKENSVILIEDNYEQRYAGFNP
NSSESYIIFEFVQDKNMEKSVKLATLIQKQFKNTAKRIDKGVHQAGFLVLRATSMPSVLVELGYISTPDEEQYLLSDAGT
TALSNSIYKAFLNYKREHDAPIGRSRVQEQELPEPENKPKESRIEIQTAEPDTATEPDKITKKSVPATQKKITGDQARTS
AKPVFKIQILVSNKILPKGSKQLKGVSPVSYYREKGLYKYTYGENTDYNKILRMKRNITPKFKDAFIIAFKNGEKMNVNE
AIKEFKKNR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=239, Percent_Identity=36.4016736401674, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87082163, Length=236, Percent_Identity=31.3559322033898, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790611, Length=261, Percent_Identity=31.0344827586207, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46296; Mature: 46296

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDRIKIIFLLCSLWLVFPCFCLAAKGKDFVVVIDPGHGGHDPGAIGRRGKEKNINLNV
CCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
ALKVGQLIQNNCNDVKVIYTRKTDVFIPLDRRAQIANNAKADLFISIHTNSIARGRTVRG
EEEEHHHHHCCCCCEEEEEEECCEEEEECCCCHHHCCCCCEEEEEEEECCCCCCCCEECC
AETYTLGLHRTEENLEVAKKENSVILIEDNYEQRYAGFNPNSSESYIIFEFVQDKNMEKS
CCEEEEEEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCCCCCEEEEEEECCCCCHHH
VKLATLIQKQFKNTAKRIDKGVHQAGFLVLRATSMPSVLVELGYISTPDEEQYLLSDAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHCCCCCCCCCCCEEECCCCC
TALSNSIYKAFLNYKREHDAPIGRSRVQEQELPEPENKPKESRIEIQTAEPDTATEPDKI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHH
TKKSVPATQKKITGDQARTSAKPVFKIQILVSNKILPKGSKQLKGVSPVSYYREKGLYKY
HHCCCCCHHHHCCCCHHHCCCCCEEEEEEEEECCCCCCCCHHCCCCCHHHHHHHCCCEEE
TYGENTDYNKILRMKRNITPKFKDAFIIAFKNGEKMNVNEAIKEFKKNR
ECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKTDRIKIIFLLCSLWLVFPCFCLAAKGKDFVVVIDPGHGGHDPGAIGRRGKEKNINLNV
CCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
ALKVGQLIQNNCNDVKVIYTRKTDVFIPLDRRAQIANNAKADLFISIHTNSIARGRTVRG
EEEEHHHHHCCCCCEEEEEEECCEEEEECCCCHHHCCCCCEEEEEEEECCCCCCCCEECC
AETYTLGLHRTEENLEVAKKENSVILIEDNYEQRYAGFNPNSSESYIIFEFVQDKNMEKS
CCEEEEEEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCCCCCEEEEEEECCCCCHHH
VKLATLIQKQFKNTAKRIDKGVHQAGFLVLRATSMPSVLVELGYISTPDEEQYLLSDAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHCCCCCCCCCCCEEECCCCC
TALSNSIYKAFLNYKREHDAPIGRSRVQEQELPEPENKPKESRIEIQTAEPDTATEPDKI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHH
TKKSVPATQKKITGDQARTSAKPVFKIQILVSNKILPKGSKQLKGVSPVSYYREKGLYKY
HHCCCCCHHHHCCCCHHHCCCCCEEEEEEEEECCCCCCCCHHCCCCCHHHHHHHCCCEEE
TYGENTDYNKILRMKRNITPKFKDAFIIAFKNGEKMNVNEAIKEFKKNR
ECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]