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The map label for this gene is nagB [C]

Identifier: 150006601

GI number: 150006601

Start: 5068439

End: 5070427

Strand: Direct

Name: nagB [C]

Synonym: BVU_4121

Alternate gene names: 150006601

Gene position: 5068439-5070427 (Clockwise)

Preceding gene: 150006600

Following gene: 150006604

Centisome position: 98.16

GC content: 47.71

Gene sequence:

>1989_bases
ATGAGAACAAACTTAAGTTCGCAGATTTCTCTGAACAGAGTATCTCCGAAATATTATAAACCCGAAAATGCGGTGGAACG
TTCTGTCTTGACCCGCTGCGAAAAAGTGCCTACCGACATCTATGAAACGATGGAGGAAGGCGTGCAGCACATTGCCAACG
AAATTACGGCAAAAATTCAGGAACGTCAGCGCGAAGGAAAGTTCTGCGTGATTGGGGCTGGAACCGGTGCTTCATTACGG
CCGCTTTATGCCGAACTAGTCCGTAAACATAAAGATGAAGGACTAAGTTTCCGTAACGTAGTAATTTTCAATTTATATGA
ATATTACCCTTTGGCTTCCGAAGGGGCTGGAAGCAGTTTCTCACAGCTGAACGACTTATTCCTAAGCCAAATCGACATCG
ATAAACAAAATGTATTCACCATCGACGGAACGATTCCGCAGGAAGCTGTCATCGAATACTGCCGCCTGTACGAACAACGT
ATCCAGACTTTTGGCGGTATAGACATCGTATTAATGGGCATTGGTCGTGAAGGCAATATTGCCATGAACGAGCCGGGATC
AAGCTTGAGCTCCCCTACCCGACTGATTTTGATTGACTCAACCTCGCGTGCTGAAGCTGCACACAACTTGGGAGTGGACA
ATCTCCCTCCCTGTTCCATCACGATGGGTGTGGCAACTATTATGGCTGCCCGCAAAATCTATTTGCTGGCATGGGGAGAT
GACAAAGCCGATATCATCAAGAAAGCGGTGGAAGACAAAGTGAGCGACACTCTGCCCGCTTCTTATCTGCAAATGCATAA
CAATGCAAATGTCTGCATAGATCTGGCAGCGGCTTCTCACCTTACCCGTATCCAACGTCCGTGGCTGGTAACCAATTGCG
AATGGAACGACAAATTGATTCGCAGCGCTATTGTATGGTTGTGTATGCGTGTCAAAAAACCGATTCTGAAACTGACCAAC
AAAGATTACAATGAAAACGGACTGAGCGAACTGCTGGCACTCTACGGTTCTGCTTACAACGTAAATATCAAAATATTCAA
TGACTTGCAGCACACCATCACCGGATGGCCCGGCGGCAAGCCCAATGCAGATGACACCTATCGCCCCGAACGGGCAAAAC
CATTCCCGAAACGCGTGGTAGTCTTCTCTCCGCACCCGGATGATGACGTAATCTCAATGGGTGGAACTTTACGGCGTTTG
GTTCAGCAAGGTCACGAAGTACACGTAGCATACGAAACTTCCGGAAACATTGCCGTAGGCGATGAAGAAGTAGTACGTTT
CATGCACTTCATCAACGGTTTCAACCAACTTTTTGATGAAAACTCCAACGAAACCATCAAAAACAAATACGCTGAAATCA
AGAAATTCCTTGCCGCCAAGAAAGAAGGCGATATGGACAGCCGCGATATCCTGACCATTAAAGGACTGATCCGTCGTGGC
GAGGCCCGTACTGCAAGTACTTACAATCAAATTCCATTGAACCGTGTCCACTTCCTCGACTTGCCGTTCTATGAAACAGG
AAAAATCGAGAAGAACCCTATCAGTGAAGCCGATGTGGAAATCGTTTTACAGTTACTACGTGACGTAAAACCCCACCAAA
TTTATGTGGCCGGTGACCTTGCCGACCCGCACGGAACCCATCGTGTCTGCACAGACGCAGTGCTTGCCGCTATCGACATC
GAAAAAGAAGCCGGTGCCGAATGGTTGAAAGATTGCCGCATCTGGATGTATCGTGGCGCATGGGCGGAATGGGAAATTGA
AAACATTGAAATGGCCGTACCGTTCAGTCCGGAAGAATTGCGTGCTAAACGTAACTCCATCTTAAAGCATCAGTCTCAGA
TGGAAAGCGCACCGTTCCTGGGCAACGACGAACGTCTGTTCTGGCAAAGAAGTGAAGACCGCAACCGGGGTACCGCTGCT
TTGTATGACCAACTTGGTTTGGCATGTTATGAAGCAATGGAGGCCTTCGTGGAATACGTACCCCTATAA

Upstream 100 bases:

>100_bases
CCAGCATGGCATCATTGTTTGTGACGAAGCCGCTACAGACGAACTGAAAGTCGGTACCTACAAATATTTCAAAGACATCG
AAAAAGCTAATTTGTAATTT

Downstream 100 bases:

>100_bases
TTTAAGAAACAACCTAACCTAATTTTATACTAACCTTTAACCAAAAAACCGAAAATCCCCGATGGATGTGATATCCCTCG
GGGATTATTTTTAGATTTCC

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-Fructose 6-phosphate; ammonia [C]

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MRTNLSSQISLNRVSPKYYKPENAVERSVLTRCEKVPTDIYETMEEGVQHIANEITAKIQERQREGKFCVIGAGTGASLR
PLYAELVRKHKDEGLSFRNVVIFNLYEYYPLASEGAGSSFSQLNDLFLSQIDIDKQNVFTIDGTIPQEAVIEYCRLYEQR
IQTFGGIDIVLMGIGREGNIAMNEPGSSLSSPTRLILIDSTSRAEAAHNLGVDNLPPCSITMGVATIMAARKIYLLAWGD
DKADIIKKAVEDKVSDTLPASYLQMHNNANVCIDLAAASHLTRIQRPWLVTNCEWNDKLIRSAIVWLCMRVKKPILKLTN
KDYNENGLSELLALYGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPFPKRVVVFSPHPDDDVISMGGTLRRL
VQQGHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQLFDENSNETIKNKYAEIKKFLAAKKEGDMDSRDILTIKGLIRRG
EARTASTYNQIPLNRVHFLDLPFYETGKIEKNPISEADVEIVLQLLRDVKPHQIYVAGDLADPHGTHRVCTDAVLAAIDI
EKEAGAEWLKDCRIWMYRGAWAEWEIENIEMAVPFSPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTAA
LYDQLGLACYEAMEAFVEYVPL

Sequences:

>Translated_662_residues
MRTNLSSQISLNRVSPKYYKPENAVERSVLTRCEKVPTDIYETMEEGVQHIANEITAKIQERQREGKFCVIGAGTGASLR
PLYAELVRKHKDEGLSFRNVVIFNLYEYYPLASEGAGSSFSQLNDLFLSQIDIDKQNVFTIDGTIPQEAVIEYCRLYEQR
IQTFGGIDIVLMGIGREGNIAMNEPGSSLSSPTRLILIDSTSRAEAAHNLGVDNLPPCSITMGVATIMAARKIYLLAWGD
DKADIIKKAVEDKVSDTLPASYLQMHNNANVCIDLAAASHLTRIQRPWLVTNCEWNDKLIRSAIVWLCMRVKKPILKLTN
KDYNENGLSELLALYGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPFPKRVVVFSPHPDDDVISMGGTLRRL
VQQGHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQLFDENSNETIKNKYAEIKKFLAAKKEGDMDSRDILTIKGLIRRG
EARTASTYNQIPLNRVHFLDLPFYETGKIEKNPISEADVEIVLQLLRDVKPHQIYVAGDLADPHGTHRVCTDAVLAAIDI
EKEAGAEWLKDCRIWMYRGAWAEWEIENIEMAVPFSPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTAA
LYDQLGLACYEAMEAFVEYVPL
>Mature_662_residues
MRTNLSSQISLNRVSPKYYKPENAVERSVLTRCEKVPTDIYETMEEGVQHIANEITAKIQERQREGKFCVIGAGTGASLR
PLYAELVRKHKDEGLSFRNVVIFNLYEYYPLASEGAGSSFSQLNDLFLSQIDIDKQNVFTIDGTIPQEAVIEYCRLYEQR
IQTFGGIDIVLMGIGREGNIAMNEPGSSLSSPTRLILIDSTSRAEAAHNLGVDNLPPCSITMGVATIMAARKIYLLAWGD
DKADIIKKAVEDKVSDTLPASYLQMHNNANVCIDLAAASHLTRIQRPWLVTNCEWNDKLIRSAIVWLCMRVKKPILKLTN
KDYNENGLSELLALYGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPFPKRVVVFSPHPDDDVISMGGTLRRL
VQQGHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQLFDENSNETIKNKYAEIKKFLAAKKEGDMDSRDILTIKGLIRRG
EARTASTYNQIPLNRVHFLDLPFYETGKIEKNPISEADVEIVLQLLRDVKPHQIYVAGDLADPHGTHRVCTDAVLAAIDI
EKEAGAEWLKDCRIWMYRGAWAEWEIENIEMAVPFSPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTAA
LYDQLGLACYEAMEAFVEYVPL

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=224, Percent_Identity=35.7142857142857, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI13027378, Length=224, Percent_Identity=34.375, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786893, Length=228, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI48994958, Length=176, Percent_Identity=26.1363636363636, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1789530, Length=205, Percent_Identity=26.3414634146341, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=237, Percent_Identity=33.7552742616034, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24581960, Length=245, Percent_Identity=32.2448979591837, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920764, Length=245, Percent_Identity=32.2448979591837, Blast_Score=116, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L [H]

EC number: 3.5.99.6 [C]

Molecular weight: Translated: 74768; Mature: 74768

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNLSSQISLNRVSPKYYKPENAVERSVLTRCEKVPTDIYETMEEGVQHIANEITAKIQ
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ERQREGKFCVIGAGTGASLRPLYAELVRKHKDEGLSFRNVVIFNLYEYYPLASEGAGSSF
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCHH
SQLNDLFLSQIDIDKQNVFTIDGTIPQEAVIEYCRLYEQRIQTFGGIDIVLMGIGREGNI
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
AMNEPGSSLSSPTRLILIDSTSRAEAAHNLGVDNLPPCSITMGVATIMAARKIYLLAWGD
EECCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEEECC
DKADIIKKAVEDKVSDTLPASYLQMHNNANVCIDLAAASHLTRIQRPWLVTNCEWNDKLI
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEECCCCCHHHH
RSAIVWLCMRVKKPILKLTNKDYNENGLSELLALYGSAYNVNIKIFNDLQHTITGWPGGK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHCCCCCCC
PNADDTYRPERAKPFPKRVVVFSPHPDDDVISMGGTLRRLVQQGHEVHVAYETSGNIAVG
CCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHCCCEEEEEEECCCCEEEC
DEEVVRFMHFINGFNQLFDENSNETIKNKYAEIKKFLAAKKEGDMDSRDILTIKGLIRRG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
EARTASTYNQIPLNRVHFLDLPFYETGKIEKNPISEADVEIVLQLLRDVKPHQIYVAGDL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
ADPHGTHRVCTDAVLAAIDIEKEAGAEWLKDCRIWMYRGAWAEWEIENIEMAVPFSPEEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCEEEECCEEEECCCCHHHH
RAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTAALYDQLGLACYEAMEAFVEYV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PL
CC
>Mature Secondary Structure
MRTNLSSQISLNRVSPKYYKPENAVERSVLTRCEKVPTDIYETMEEGVQHIANEITAKIQ
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ERQREGKFCVIGAGTGASLRPLYAELVRKHKDEGLSFRNVVIFNLYEYYPLASEGAGSSF
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCHH
SQLNDLFLSQIDIDKQNVFTIDGTIPQEAVIEYCRLYEQRIQTFGGIDIVLMGIGREGNI
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
AMNEPGSSLSSPTRLILIDSTSRAEAAHNLGVDNLPPCSITMGVATIMAARKIYLLAWGD
EECCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEEECC
DKADIIKKAVEDKVSDTLPASYLQMHNNANVCIDLAAASHLTRIQRPWLVTNCEWNDKLI
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEECCCCCHHHH
RSAIVWLCMRVKKPILKLTNKDYNENGLSELLALYGSAYNVNIKIFNDLQHTITGWPGGK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHCCCCCCC
PNADDTYRPERAKPFPKRVVVFSPHPDDDVISMGGTLRRLVQQGHEVHVAYETSGNIAVG
CCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHCCCEEEEEEECCCCEEEC
DEEVVRFMHFINGFNQLFDENSNETIKNKYAEIKKFLAAKKEGDMDSRDILTIKGLIRRG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
EARTASTYNQIPLNRVHFLDLPFYETGKIEKNPISEADVEIVLQLLRDVKPHQIYVAGDL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
ADPHGTHRVCTDAVLAAIDIEKEAGAEWLKDCRIWMYRGAWAEWEIENIEMAVPFSPEEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCEEEECCEEEECCCCHHHH
RAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTAALYDQLGLACYEAMEAFVEYV
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Glucosamine 6-phosphate; H2O [C]

Specific reaction: D-Glucosamine 6-phosphate + H2O --> D-Fructose 6-phosphate + ammonia [C]

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]