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The map label for this gene is rfbF [H]

Identifier: 150006508

GI number: 150006508

Start: 4960093

End: 4960893

Strand: Reverse

Name: rfbF [H]

Synonym: BVU_4028

Alternate gene names: 150006508

Gene position: 4960893-4960093 (Counterclockwise)

Preceding gene: 150006509

Following gene: 150006507

Centisome position: 96.08

GC content: 34.96

Gene sequence:

>801_bases
ATGAAGGTAGTTCTTTTAGCAGGCGGTTTTGGCTCGCGTATTAGTGAAGAGAGCCAATTTAAGCCAAAGCCCATGATTGA
AATTTGCGGTATGCCCATTCTTTGGCATATAATGAAGGAATATGCATATTATGGATATAATGAATTCATAATTTGTGCTG
GATACAAACAGGAATATATTAAGGAATGGTTTGCAAACTATTTTCTTTACAATTCCGATGTTAGCTTCAACTTCCGTAAC
GGTAAGAATGAAATAACTGTACATCATTCAAATCTTGAACCTTGGAAAGTAACGATAGTTGATACAGGATATAACACATT
GACCGGAGGCCGTATCAAACGTATAAAGGAATATGTTAACAATGAAACCTTTATGATGACATATGGTGATGGTGTGTGTG
ATGTTGACATCAATAAGCTTGTGGCATTTCATAAAAAGCATGGTAAAATAGCAACTCTTACTGCTGTTATTCAAGCACAA
GACAAGGGGGTTTTAAATATTGATGAAAATAATTCCATTCGTTCATTTCGTGAGAAGAATATCAATGATGGTGTACCTAT
TAATGCCGGATATATGGTGTTGGAACCTAGAGTGTTCGATTATATAGAAGGAGATCAAACCATATTTGAAAAAGAACCAT
TGAATAAGTTGGCTACCGAAGGTGAACTAATGTCTTATACACATAAGGGGTTCTGGCAATGTATGGATAATATACGTGAA
AAAAATCTATTGGAACAGTTGCTTATTGATGGAGTTGCTCCATGGAAAAAATGGGAAAGAGAAATACCGATAATAAAATG
A

Upstream 100 bases:

>100_bases
TAAATGTAGCCAATATTCCTCATGATCCTCTAGAGCAGGTAAACATTCCAATAGAAGAGAGTGATATAAAGTTTGATTTC
AACAAAATTAGCAGATCTAT

Downstream 100 bases:

>100_bases
TGATGTTTATGAGAAATATTAGATTGTCCAAATCTTGCATTGGTAAGGAAGAAATAGATGCCGTAACAGACGTTCTTCAT
AGAGAGTATTTGGGTATGGG

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKVVLLAGGFGSRISEESQFKPKPMIEICGMPILWHIMKEYAYYGYNEFIICAGYKQEYIKEWFANYFLYNSDVSFNFRN
GKNEITVHHSNLEPWKVTIVDTGYNTLTGGRIKRIKEYVNNETFMMTYGDGVCDVDINKLVAFHKKHGKIATLTAVIQAQ
DKGVLNIDENNSIRSFREKNINDGVPINAGYMVLEPRVFDYIEGDQTIFEKEPLNKLATEGELMSYTHKGFWQCMDNIRE
KNLLEQLLIDGVAPWKKWEREIPIIK

Sequences:

>Translated_266_residues
MKVVLLAGGFGSRISEESQFKPKPMIEICGMPILWHIMKEYAYYGYNEFIICAGYKQEYIKEWFANYFLYNSDVSFNFRN
GKNEITVHHSNLEPWKVTIVDTGYNTLTGGRIKRIKEYVNNETFMMTYGDGVCDVDINKLVAFHKKHGKIATLTAVIQAQ
DKGVLNIDENNSIRSFREKNINDGVPINAGYMVLEPRVFDYIEGDQTIFEKEPLNKLATEGELMSYTHKGFWQCMDNIRE
KNLLEQLLIDGVAPWKKWEREIPIIK
>Mature_266_residues
MKVVLLAGGFGSRISEESQFKPKPMIEICGMPILWHIMKEYAYYGYNEFIICAGYKQEYIKEWFANYFLYNSDVSFNFRN
GKNEITVHHSNLEPWKVTIVDTGYNTLTGGRIKRIKEYVNNETFMMTYGDGVCDVDINKLVAFHKKHGKIATLTAVIQAQ
DKGVLNIDENNSIRSFREKNINDGVPINAGYMVLEPRVFDYIEGDQTIFEKEPLNKLATEGELMSYTHKGFWQCMDNIRE
KNLLEQLLIDGVAPWKKWEREIPIIK

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI133931050, Length=252, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320148, Length=253, Percent_Identity=28.8537549407115, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355443, Length=255, Percent_Identity=27.4509803921569, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644084, Length=255, Percent_Identity=27.4509803921569, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 30879; Mature: 30879

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLLAGGFGSRISEESQFKPKPMIEICGMPILWHIMKEYAYYGYNEFIICAGYKQEYI
CEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHH
KEWFANYFLYNSDVSFNFRNGKNEITVHHSNLEPWKVTIVDTGYNTLTGGRIKRIKEYVN
HHHHHHHEEECCCEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHC
NETFMMTYGDGVCDVDINKLVAFHKKHGKIATLTAVIQAQDKGVLNIDENNSIRSFREKN
CCEEEEEECCCEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHCC
INDGVPINAGYMVLEPRVFDYIEGDQTIFEKEPLNKLATEGELMSYTHKGFWQCMDNIRE
CCCCCCCCCCEEEECCHHHHHCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KNLLEQLLIDGVAPWKKWEREIPIIK
HHHHHHHHHCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKVVLLAGGFGSRISEESQFKPKPMIEICGMPILWHIMKEYAYYGYNEFIICAGYKQEYI
CEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHH
KEWFANYFLYNSDVSFNFRNGKNEITVHHSNLEPWKVTIVDTGYNTLTGGRIKRIKEYVN
HHHHHHHEEECCCEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHC
NETFMMTYGDGVCDVDINKLVAFHKKHGKIATLTAVIQAQDKGVLNIDENNSIRSFREKN
CCEEEEEECCCEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHCC
INDGVPINAGYMVLEPRVFDYIEGDQTIFEKEPLNKLATEGELMSYTHKGFWQCMDNIRE
CCCCCCCCCCEEEECCHHHHHCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KNLLEQLLIDGVAPWKKWEREIPIIK
HHHHHHHHHCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]