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The map label for this gene is asnB [H]

Identifier: 150006490

GI number: 150006490

Start: 4940926

End: 4942596

Strand: Direct

Name: asnB [H]

Synonym: BVU_4009

Alternate gene names: 150006490

Gene position: 4940926-4942596 (Clockwise)

Preceding gene: 150006489

Following gene: 150006491

Centisome position: 95.7

GC content: 52.18

Gene sequence:

>1671_bases
ATGTGTGGAATCGTAGGAATATTCAAGATAAAGCAACAAACGCAGGAACTGCGTCAGAAGGCATTGAAAATGTCACAAAA
GCTCCGCCACCGCGGGCCGGACTGGAGCGGCATCTACGTAGGCGACTCTGCCATCCTGGCCCATGAACGCCTCTCCATCG
TAGACCCTCAAAGCGGAGGGCAACCCCTATATAGTCCCGACCGGAAACAAATCCTCGCCGTGAACGGAGAAATCTACAAC
CATCGGGATGTTCGTGCAAAGTACGCCGAAAAATATGATTTCCATACCGGAAGCGATTGCGAAGTCATCCTTGCCCTTTA
CCGTGACAAAGGTATTCACTTCCTCGAAGAGCTGAACGGCATCTTCGCCTTCGCCCTTTATGATGAGGAGAAAGACGATT
TCCTCATTGCCCGCGACCCCATCGGAGTCATACCTTTATATATAGGTAAGGATAAGGACGGCAAAATATACTGCGCCAGC
GAGTTGAAAGCCCTCGAAGGGTTCTGTGATGAATACGAACCCTTCCTTCCCGGCCATTACTATTGGGGAAAAGAAGGCAA
GATGACCCGCTGGTACGTCCGCGACTGGTTTGAATACGAAGCCGTGAAAAACAACAATGCCTATTCCCTGGACATACACG
ACGGACTGGAAGAGGCCGTTAAACGCCAACTGATGAGTGATGTGCCCTACGGCGTACTTCTTTCCGGCGGTCTGGACAGT
TCCGTCATCTCTGCCATTGCCAAGAAATATGCAGGCAAACGGGTAGAAACCGACAATAAAAAAGATGCCTGGTGGCCTCA
GCTCCATTCCTTTGCCATCGGTCTGGAAGGTGCGCCCGACCTGATAAAAGCCCGCGAAGTGGCCCGCTTTATCGGAACCG
TGCATCATGAAATCCACTATACCATCCAAGAAGGATTGGACGCTATCCGCGACGTCATTTACTACATCGAGACTTACGAT
GTCACCACCGTTCGCGCCTCTACCCCGATGTATTTGCTGGCACGCGTCATTAAAAGCATGGGAATCAAAATGGTGCTGAG
TGGCGAAGGGGCTGATGAAGTGTTCGGCGGCTATCTCTATTTCCACAAAGCCCCTACAGCCCAAGCCTTTCATGAAGAAA
CCGTGCGTAAACTAGGCAAACTCCACCTCTATGACTGCCTCCGTGCCAATAAATCCCTCGCAGCATGGGGAGTGGAAGGC
CGAGTGCCTTTCCTGGACAAAGATTTTCTGGACATAGCCATGCGCCTCAATCCCGAGGCAAAGATGTGCCCCGGAAGCAC
CATCGAAAAGAAAATAGTCCGCAAAGCCTTTGCAGACATGTTGCCCGACAGTGTAGCATGGCGACAGAAAGAGCAGTTCA
GCGACGGCGTGGGCTATAGTTGGATTGATACACTGAAAGCCCTTACCGCCGAAGCCGTCAGTGACGAGCAAATGGCGCAC
GCAGCCGAACGGTTTCCTATCAATACGCCGCAAAACAAGGAAGAATACTACTATCGCAGCATCTTCCAGGAGCACTTTCC
CAGTGAAAGCGCAGCCCGCAGCGTGCCAAGCGTGCCCAGTGTGGCATGTTCTACAGCCGAAGCCCTTGCGTGGGACGCCG
CCTTCAAGAACATGAACGAACCAAGCGGACGGGCCGTGAAGGGAGTGCACGAAGAAGCATATGATTCATAA

Upstream 100 bases:

>100_bases
GCAAGCGGAGCCAGCCTGGTGGTACGCGCCATTGCCAGCGGACGGAAAGCGGCAACGGACATAGACAGTTATCTCAATAA
ATAAATCAACAAATACAATT

Downstream 100 bases:

>100_bases
TATGGCAACGTGAGTCTGATTCCAACAATTTCATGCATACAAAGAAGAGATGCTTTGCACCCCGAAATCTTGCGCGGCCT
TGCCTTCGAAAAGAGGAAGG

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MCGIVGIFKIKQQTQELRQKALKMSQKLRHRGPDWSGIYVGDSAILAHERLSIVDPQSGGQPLYSPDRKQILAVNGEIYN
HRDVRAKYAEKYDFHTGSDCEVILALYRDKGIHFLEELNGIFAFALYDEEKDDFLIARDPIGVIPLYIGKDKDGKIYCAS
ELKALEGFCDEYEPFLPGHYYWGKEGKMTRWYVRDWFEYEAVKNNNAYSLDIHDGLEEAVKRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAGKRVETDNKKDAWWPQLHSFAIGLEGAPDLIKAREVARFIGTVHHEIHYTIQEGLDAIRDVIYYIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTAQAFHEETVRKLGKLHLYDCLRANKSLAAWGVEG
RVPFLDKDFLDIAMRLNPEAKMCPGSTIEKKIVRKAFADMLPDSVAWRQKEQFSDGVGYSWIDTLKALTAEAVSDEQMAH
AAERFPINTPQNKEEYYYRSIFQEHFPSESAARSVPSVPSVACSTAEALAWDAAFKNMNEPSGRAVKGVHEEAYDS

Sequences:

>Translated_556_residues
MCGIVGIFKIKQQTQELRQKALKMSQKLRHRGPDWSGIYVGDSAILAHERLSIVDPQSGGQPLYSPDRKQILAVNGEIYN
HRDVRAKYAEKYDFHTGSDCEVILALYRDKGIHFLEELNGIFAFALYDEEKDDFLIARDPIGVIPLYIGKDKDGKIYCAS
ELKALEGFCDEYEPFLPGHYYWGKEGKMTRWYVRDWFEYEAVKNNNAYSLDIHDGLEEAVKRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAGKRVETDNKKDAWWPQLHSFAIGLEGAPDLIKAREVARFIGTVHHEIHYTIQEGLDAIRDVIYYIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTAQAFHEETVRKLGKLHLYDCLRANKSLAAWGVEG
RVPFLDKDFLDIAMRLNPEAKMCPGSTIEKKIVRKAFADMLPDSVAWRQKEQFSDGVGYSWIDTLKALTAEAVSDEQMAH
AAERFPINTPQNKEEYYYRSIFQEHFPSESAARSVPSVPSVACSTAEALAWDAAFKNMNEPSGRAVKGVHEEAYDS
>Mature_556_residues
MCGIVGIFKIKQQTQELRQKALKMSQKLRHRGPDWSGIYVGDSAILAHERLSIVDPQSGGQPLYSPDRKQILAVNGEIYN
HRDVRAKYAEKYDFHTGSDCEVILALYRDKGIHFLEELNGIFAFALYDEEKDDFLIARDPIGVIPLYIGKDKDGKIYCAS
ELKALEGFCDEYEPFLPGHYYWGKEGKMTRWYVRDWFEYEAVKNNNAYSLDIHDGLEEAVKRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAGKRVETDNKKDAWWPQLHSFAIGLEGAPDLIKAREVARFIGTVHHEIHYTIQEGLDAIRDVIYYIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTAQAFHEETVRKLGKLHLYDCLRANKSLAAWGVEG
RVPFLDKDFLDIAMRLNPEAKMCPGSTIEKKIVRKAFADMLPDSVAWRQKEQFSDGVGYSWIDTLKALTAEAVSDEQMAH
AAERFPINTPQNKEEYYYRSIFQEHFPSESAARSVPSVPSVACSTAEALAWDAAFKNMNEPSGRAVKGVHEEAYDS

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=552, Percent_Identity=38.0434782608696, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI168229252, Length=552, Percent_Identity=38.0434782608696, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI168229250, Length=552, Percent_Identity=38.0434782608696, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI296010848, Length=526, Percent_Identity=38.9733840304183, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI296010852, Length=458, Percent_Identity=39.9563318777293, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI296010850, Length=458, Percent_Identity=39.9563318777293, Blast_Score=288, Evalue=7e-78,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=65.3429602888087, Blast_Score=772, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=573, Percent_Identity=44.5026178010471, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71993933, Length=535, Percent_Identity=45.981308411215, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17560178, Length=519, Percent_Identity=35.8381502890173, Blast_Score=287, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI25147560, Length=351, Percent_Identity=38.4615384615385, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321563, Length=583, Percent_Identity=48.7135506003431, Blast_Score=525, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325403, Length=584, Percent_Identity=46.9178082191781, Blast_Score=513, Evalue=1e-146,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=47.6603119584055, Blast_Score=486, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62872; Mature: 62872

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIFKIKQQTQELRQKALKMSQKLRHRGPDWSGIYVGDSAILAHERLSIVDPQSGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEHHCCCEEECCCCCC
QPLYSPDRKQILAVNGEIYNHRDVRAKYAEKYDFHTGSDCEVILALYRDKGIHFLEELNG
CCCCCCCCCEEEEECCCEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCC
IFAFALYDEEKDDFLIARDPIGVIPLYIGKDKDGKIYCASELKALEGFCDEYEPFLPGHY
EEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCE
YWGKEGKMTRWYVRDWFEYEAVKNNNAYSLDIHDGLEEAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAIAKKYAGKRVETDNKKDAWWPQLHSFAIGLEGAPDLIKAREVARFIGTVHHEIHY
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYYIETYDVTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLY
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPTAQAFHEETVRKLGKLHLYDCLRANKSLAAWGVEGRVPFLDKDFLDIAMRLNPEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
KMCPGSTIEKKIVRKAFADMLPDSVAWRQKEQFSDGVGYSWIDTLKALTAEAVSDEQMAH
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHH
AAERFPINTPQNKEEYYYRSIFQEHFPSESAARSVPSVPSVACSTAEALAWDAAFKNMNE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSGRAVKGVHEEAYDS
CCCCHHHCHHHHHCCC
>Mature Secondary Structure
MCGIVGIFKIKQQTQELRQKALKMSQKLRHRGPDWSGIYVGDSAILAHERLSIVDPQSGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEHHCCCEEECCCCCC
QPLYSPDRKQILAVNGEIYNHRDVRAKYAEKYDFHTGSDCEVILALYRDKGIHFLEELNG
CCCCCCCCCEEEEECCCEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCC
IFAFALYDEEKDDFLIARDPIGVIPLYIGKDKDGKIYCASELKALEGFCDEYEPFLPGHY
EEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCE
YWGKEGKMTRWYVRDWFEYEAVKNNNAYSLDIHDGLEEAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAIAKKYAGKRVETDNKKDAWWPQLHSFAIGLEGAPDLIKAREVARFIGTVHHEIHY
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYYIETYDVTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLY
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPTAQAFHEETVRKLGKLHLYDCLRANKSLAAWGVEGRVPFLDKDFLDIAMRLNPEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
KMCPGSTIEKKIVRKAFADMLPDSVAWRQKEQFSDGVGYSWIDTLKALTAEAVSDEQMAH
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHH
AAERFPINTPQNKEEYYYRSIFQEHFPSESAARSVPSVPSVACSTAEALAWDAAFKNMNE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSGRAVKGVHEEAYDS
CCCCHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]