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The map label for this gene is gltB [H]

Identifier: 150006489

GI number: 150006489

Start: 4939569

End: 4940909

Strand: Direct

Name: gltB [H]

Synonym: BVU_4008

Alternate gene names: 150006489

Gene position: 4939569-4940909 (Clockwise)

Preceding gene: 150006488

Following gene: 150006490

Centisome position: 95.67

GC content: 56.6

Gene sequence:

>1341_bases
ATGGGAAATCCAAAAGCATTTTTAACTATACCGCGTCAGGAAGCGGGCTACCGTCCCATCCACGACCGGATATCAGATTA
TAGCGAGGTAGAACAGACTCTGAACAGCCGTGACCGGAAACTTCAAGCCTCACGCTGCATGGACTGCGGCGTTCCCTTCT
GCCACTGGGCTTGTCCGCTGGGCAACAAGCAGCCCGAATTTCAAGATGCCCTCTACAAAGGCAAATGGGAGGAAGCCTAC
AAGATATTGAACGAAACCAACGATTTTCCTGAGTTCACCGGCCGCATCTGCCCCGCCCTCTGCGAAAAGAGTTGCGTGCT
GAAACTGAGCATGGACTCTCCCGTCACCATCCGCGAGAACGAAGCGGCCATTGCCGAAGCAGCCTTCCGCGAAGGTTACA
TCAAGCCCCGGAACATTGAACGCAACGGACGGAAAGTAGCCATTATCGGCGCCGGGCCCGCCGGACTGGCCGCCGCCAAC
CAGCTCAACGGAAAAGGGTACACAGTGACCGTCTTCGACAAGAATGAGGCTCCCGGCGGCCTGCTACGTTACGGCATCCC
CAACTTCAAGCTGCACAAGCCCATCATCGACCGCCGTATCCGGGTGATGGAAGAAGAAGGCATCCACTTCAAAATGAACA
ACGATGTGGATGTGCGGCAACTCCCCGAAGGTTTTGACGCCTACTGCATCTGCACCGGAGCTCCGACCGCCCGTGACCTC
CCTGTCCCCGGACGCGAACTGAAAGGCATCCACCCGGCACTGGACATGCTGGCGCAACAAAACCGTATCCTTGCCGGCAT
GACGTTCCCGAAAGAACAACTGGTTACTGCCAAAGGGAAAAAAGTACTGGTCATCGGCGGAGGGGATACCGGCAGCGACT
GCATAGGGACCAGCAACCGGCAAGGTGCCCTAAGCGTCACCCAAATAGAAATCATGCCCCAGCCGCCCGTAGGACAGAAT
CCTGCCACTCCATGGCCCCAATTCCCCGTCGTACTGAAAACCACCTCTTCTCACGAAGAAGGGTGCAGCCGCCTATGGTC
TCTCGCCACCCGTAAGTTTTTAGGCAAAAACGGTAAAGTGTGCGGTGTGGAAGTGGAACCGGTGGAATGGACTCCGAGTC
CCGACGGCGGCCGCCCTGTCATGAAGCCCACCGGAAAGGTGGAAGTGATTGAAGCCGACCTGGTTCTTCTGGCAATGGGA
TTCCTAAAGCCCGAACATCCCCAATTCGCCGAAAACGTATTCGTAGCCGGCGATGCAGCAAGCGGAGCCAGCCTGGTGGT
ACGCGCCATTGCCAGCGGACGGAAAGCGGCAACGGACATAGACAGTTATCTCAATAAATAA

Upstream 100 bases:

>100_bases
ATATAAACGAGTATTGCAAGAAGAACAAATGCGTAAGCTGCAACAAAAAATAGCTGATGTACAAAGAGATTATTAACGAC
AAAAGAAATACAACAAAGAT

Downstream 100 bases:

>100_bases
ATCAACAAATACAATTATGTGTGGAATCGTAGGAATATTCAAGATAAAGCAACAAACGCAGGAACTGCGTCAGAAGGCAT
TGAAAATGTCACAAAAGCTC

Product: glutamate synthase, small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MGNPKAFLTIPRQEAGYRPIHDRISDYSEVEQTLNSRDRKLQASRCMDCGVPFCHWACPLGNKQPEFQDALYKGKWEEAY
KILNETNDFPEFTGRICPALCEKSCVLKLSMDSPVTIRENEAAIAEAAFREGYIKPRNIERNGRKVAIIGAGPAGLAAAN
QLNGKGYTVTVFDKNEAPGGLLRYGIPNFKLHKPIIDRRIRVMEEEGIHFKMNNDVDVRQLPEGFDAYCICTGAPTARDL
PVPGRELKGIHPALDMLAQQNRILAGMTFPKEQLVTAKGKKVLVIGGGDTGSDCIGTSNRQGALSVTQIEIMPQPPVGQN
PATPWPQFPVVLKTTSSHEEGCSRLWSLATRKFLGKNGKVCGVEVEPVEWTPSPDGGRPVMKPTGKVEVIEADLVLLAMG
FLKPEHPQFAENVFVAGDAASGASLVVRAIASGRKAATDIDSYLNK

Sequences:

>Translated_446_residues
MGNPKAFLTIPRQEAGYRPIHDRISDYSEVEQTLNSRDRKLQASRCMDCGVPFCHWACPLGNKQPEFQDALYKGKWEEAY
KILNETNDFPEFTGRICPALCEKSCVLKLSMDSPVTIRENEAAIAEAAFREGYIKPRNIERNGRKVAIIGAGPAGLAAAN
QLNGKGYTVTVFDKNEAPGGLLRYGIPNFKLHKPIIDRRIRVMEEEGIHFKMNNDVDVRQLPEGFDAYCICTGAPTARDL
PVPGRELKGIHPALDMLAQQNRILAGMTFPKEQLVTAKGKKVLVIGGGDTGSDCIGTSNRQGALSVTQIEIMPQPPVGQN
PATPWPQFPVVLKTTSSHEEGCSRLWSLATRKFLGKNGKVCGVEVEPVEWTPSPDGGRPVMKPTGKVEVIEADLVLLAMG
FLKPEHPQFAENVFVAGDAASGASLVVRAIASGRKAATDIDSYLNK
>Mature_445_residues
GNPKAFLTIPRQEAGYRPIHDRISDYSEVEQTLNSRDRKLQASRCMDCGVPFCHWACPLGNKQPEFQDALYKGKWEEAYK
ILNETNDFPEFTGRICPALCEKSCVLKLSMDSPVTIRENEAAIAEAAFREGYIKPRNIERNGRKVAIIGAGPAGLAAANQ
LNGKGYTVTVFDKNEAPGGLLRYGIPNFKLHKPIIDRRIRVMEEEGIHFKMNNDVDVRQLPEGFDAYCICTGAPTARDLP
VPGRELKGIHPALDMLAQQNRILAGMTFPKEQLVTAKGKKVLVIGGGDTGSDCIGTSNRQGALSVTQIEIMPQPPVGQNP
ATPWPQFPVVLKTTSSHEEGCSRLWSLATRKFLGKNGKVCGVEVEPVEWTPSPDGGRPVMKPTGKVEVIEADLVLLAMGF
LKPEHPQFAENVFVAGDAASGASLVVRAIASGRKAATDIDSYLNK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=466, Percent_Identity=24.6781115879828, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789606, Length=485, Percent_Identity=32.7835051546392, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=31.2236286919831, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082180, Length=466, Percent_Identity=30.4721030042918, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788468, Length=418, Percent_Identity=27.511961722488, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789243, Length=252, Percent_Identity=29.7619047619048, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17570289, Length=485, Percent_Identity=40.8247422680412, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI71984108, Length=473, Percent_Identity=24.7357293868922, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=175, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=38.75, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=44.2148760330579, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=44.2148760330579, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=44.2148760330579, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=44.2148760330579, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640763, Length=478, Percent_Identity=22.8033472803347, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18858217, Length=478, Percent_Identity=22.8033472803347, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 48724; Mature: 48593

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNPKAFLTIPRQEAGYRPIHDRISDYSEVEQTLNSRDRKLQASRCMDCGVPFCHWACPL
CCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCC
GNKQPEFQDALYKGKWEEAYKILNETNDFPEFTGRICPALCEKSCVLKLSMDSPVTIREN
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
EAAIAEAAFREGYIKPRNIERNGRKVAIIGAGPAGLAAANQLNGKGYTVTVFDKNEAPGG
CHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCCCC
LLRYGIPNFKLHKPIIDRRIRVMEEEGIHFKMNNDVDVRQLPEGFDAYCICTGAPTARDL
EEECCCCCCEECCHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCEEEEECCCCCCCCC
PVPGRELKGIHPALDMLAQQNRILAGMTFPKEQLVTAKGKKVLVIGGGDTGSDCIGTSNR
CCCCHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
QGALSVTQIEIMPQPPVGQNPATPWPQFPVVLKTTSSHEEGCSRLWSLATRKFLGKNGKV
CCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
CGVEVEPVEWTPSPDGGRPVMKPTGKVEVIEADLVLLAMGFLKPEHPQFAENVFVAGDAA
EEEEECCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCEEEECCCC
SGASLVVRAIASGRKAATDIDSYLNK
CCHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GNPKAFLTIPRQEAGYRPIHDRISDYSEVEQTLNSRDRKLQASRCMDCGVPFCHWACPL
CCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCC
GNKQPEFQDALYKGKWEEAYKILNETNDFPEFTGRICPALCEKSCVLKLSMDSPVTIREN
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
EAAIAEAAFREGYIKPRNIERNGRKVAIIGAGPAGLAAANQLNGKGYTVTVFDKNEAPGG
CHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCCCC
LLRYGIPNFKLHKPIIDRRIRVMEEEGIHFKMNNDVDVRQLPEGFDAYCICTGAPTARDL
EEECCCCCCEECCHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCEEEEECCCCCCCCC
PVPGRELKGIHPALDMLAQQNRILAGMTFPKEQLVTAKGKKVLVIGGGDTGSDCIGTSNR
CCCCHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
QGALSVTQIEIMPQPPVGQNPATPWPQFPVVLKTTSSHEEGCSRLWSLATRKFLGKNGKV
CCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
CGVEVEPVEWTPSPDGGRPVMKPTGKVEVIEADLVLLAMGFLKPEHPQFAENVFVAGDAA
EEEEECCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCEEEECCCC
SGASLVVRAIASGRKAATDIDSYLNK
CCHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]