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The map label for this gene is dapF

Identifier: 150006487

GI number: 150006487

Start: 4933700

End: 4934542

Strand: Reverse

Name: dapF

Synonym: BVU_4006

Alternate gene names: 150006487

Gene position: 4934542-4933700 (Counterclockwise)

Preceding gene: 150006493

Following gene: 150006486

Centisome position: 95.57

GC content: 49.58

Gene sequence:

>843_bases
ATGGCAACAACTATCAAGTTCACCAAGATGCAAGGAACCGGCAATGATTATATCTATGTAAATACCTTGTCTTCTCCGCT
GCAAGATCCGATAAAAGCCGCCAGAAAATGGAGCACTTATCATACCGGTATCGGTGCGGACGGTCTGGTACTGATTGGAG
CATCCGAAAAAGCGGATTTCAGTATGCGTATATTCAATGCAGACGGCTCCGAAGCCATGATGTGTGGAAACGCCTCGCGC
TGCATAGGCAAATATGTATACGAGAAAGGACTGACGGATAAGGAGGTGATCACGCTGGAAACCCTTTCGGGAATCAAAAT
CCTGAAACTTCATACAGGCAACGGGGTGGTGAAGGATGTAACCGTAGATATGGGTACGCCTCTGCTATCGAATCCCGGAC
AGATAGCTACGAAAACCGGCGGAATGCTGGCAGAAACCATCCCGGCCGATGGAAAAGAATACAAAGGAACATTCGTCTGC
ATGGGAAATCCGCATGTTGTCATTTTCGTAGATGATATAAAAGAGGTGGATTTGCCCGCGGTTGGCCCGAAACTGGAAAA
TCATCCGCTTTTTCCTGAGCGGACCAATGTGGAATTTGTAGAAATATTGCCGGATCAATCCCTACGGATGAGAGTGTGGG
AAAGAGGTTCGGGCATCACAATGGCTTGCGGAACAGGGGCCTGCGCCACCGCCGTGGCGGCCGTACTGAACCATAAAGCC
GGACGAAAATCGTGGGTAAGGATGGACGGAGGCGATCTCCACATCCATTGGAACGAGAAAGACGGACATGTATACATGAC
CGGTCCGGCCGGAAAGGTTTTCGAAGGCGAAATAGAGATGTGA

Upstream 100 bases:

>100_bases
AGAAAATAGAAACAATTATATCTTATAAAATATCAAAAAGATATATATTTGCACAGAATTAAAAGCAAAAGGAAACCACA
ATAAATAAGTATTAATCAAA

Downstream 100 bases:

>100_bases
TACTGATTTTGAAAAACTTAAAACAACGATAAAAACAATAACAACGATGGCACTAGTAAACGAACATTTTTTAAAACTAC
CAAACAATTACTTGTTTTCG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MATTIKFTKMQGTGNDYIYVNTLSSPLQDPIKAARKWSTYHTGIGADGLVLIGASEKADFSMRIFNADGSEAMMCGNASR
CIGKYVYEKGLTDKEVITLETLSGIKILKLHTGNGVVKDVTVDMGTPLLSNPGQIATKTGGMLAETIPADGKEYKGTFVC
MGNPHVVIFVDDIKEVDLPAVGPKLENHPLFPERTNVEFVEILPDQSLRMRVWERGSGITMACGTGACATAVAAVLNHKA
GRKSWVRMDGGDLHIHWNEKDGHVYMTGPAGKVFEGEIEM

Sequences:

>Translated_280_residues
MATTIKFTKMQGTGNDYIYVNTLSSPLQDPIKAARKWSTYHTGIGADGLVLIGASEKADFSMRIFNADGSEAMMCGNASR
CIGKYVYEKGLTDKEVITLETLSGIKILKLHTGNGVVKDVTVDMGTPLLSNPGQIATKTGGMLAETIPADGKEYKGTFVC
MGNPHVVIFVDDIKEVDLPAVGPKLENHPLFPERTNVEFVEILPDQSLRMRVWERGSGITMACGTGACATAVAAVLNHKA
GRKSWVRMDGGDLHIHWNEKDGHVYMTGPAGKVFEGEIEM
>Mature_279_residues
ATTIKFTKMQGTGNDYIYVNTLSSPLQDPIKAARKWSTYHTGIGADGLVLIGASEKADFSMRIFNADGSEAMMCGNASRC
IGKYVYEKGLTDKEVITLETLSGIKILKLHTGNGVVKDVTVDMGTPLLSNPGQIATKTGGMLAETIPADGKEYKGTFVCM
GNPHVVIFVDDIKEVDLPAVGPKLENHPLFPERTNVEFVEILPDQSLRMRVWERGSGITMACGTGACATAVAAVLNHKAG
RKSWVRMDGGDLHIHWNEKDGHVYMTGPAGKVFEGEIEM

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=35.8156028368794, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BACV8 (A6L7E5)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301231.1
- ProteinModelPortal:   A6L7E5
- SMR:   A6L7E5
- STRING:   A6L7E5
- GeneID:   5304965
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4006
- NMPDR:   fig|435590.6.peg.3721
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30295; Mature: 30164

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 223-223

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTIKFTKMQGTGNDYIYVNTLSSPLQDPIKAARKWSTYHTGIGADGLVLIGASEKADF
CCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCE
SMRIFNADGSEAMMCGNASRCIGKYVYEKGLTDKEVITLETLSGIKILKLHTGNGVVKDV
EEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCEEEEE
TVDMGTPLLSNPGQIATKTGGMLAETIPADGKEYKGTFVCMGNPHVVIFVDDIKEVDLPA
EEECCCCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
VGPKLENHPLFPERTNVEFVEILPDQSLRMRVWERGSGITMACGTGACATAVAAVLNHKA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCC
GRKSWVRMDGGDLHIHWNEKDGHVYMTGPAGKVFEGEIEM
CCCEEEEECCCEEEEEEECCCCEEEEECCCCCEEECCCCC
>Mature Secondary Structure 
ATTIKFTKMQGTGNDYIYVNTLSSPLQDPIKAARKWSTYHTGIGADGLVLIGASEKADF
CCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCE
SMRIFNADGSEAMMCGNASRCIGKYVYEKGLTDKEVITLETLSGIKILKLHTGNGVVKDV
EEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCEEEEE
TVDMGTPLLSNPGQIATKTGGMLAETIPADGKEYKGTFVCMGNPHVVIFVDDIKEVDLPA
EEECCCCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
VGPKLENHPLFPERTNVEFVEILPDQSLRMRVWERGSGITMACGTGACATAVAAVLNHKA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCC
GRKSWVRMDGGDLHIHWNEKDGHVYMTGPAGKVFEGEIEM
CCCEEEEECCCEEEEEEECCCCEEEEECCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA