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The map label for this gene is dapL

Identifier: 150006486

GI number: 150006486

Start: 4932424

End: 4933653

Strand: Reverse

Name: dapL

Synonym: BVU_4005

Alternate gene names: 150006486

Gene position: 4933653-4932424 (Counterclockwise)

Preceding gene: 150006487

Following gene: 150006485

Centisome position: 95.55

GC content: 51.54

Gene sequence:

>1230_bases
ATGGCACTAGTAAACGAACATTTTTTAAAACTACCAAACAATTACTTGTTTTCGGACATTGCCAAAAAGGTAAATGCATT
CAAAGTCTCTCATCCCAAAACGGACCTTATCCGTCTGGGTATCGGAGATGTAACCCGCCCGCTGCCTCAAACTTCCATCG
AAGCCATGTATAAGGCAGTAGACGAGCTGGCAAACAAAGAGACATTCCACGGTTACGGTCCCGAACAAGGTTATGATTTC
CTGATAGATGCCGTCATCAGGAACGATTACGCTCCGCGCGGCGTCTATCTGGAACCCGGCGAAGTCTTTATCAGTGACGG
CGCCAAGAGCGATACGGGAAATATCGGAGACATCCTTCGCCACGACAACAGCATCGGCGTGACAGACCCCATCTACCCCG
TATACATCGACTCGAACGTAATGTGCGGCCGTGCCGGGATACTGGAAGACGGGCGATGGAGCAACGTGGTATACTTGCCT
TGCCTGAGCGAGAATAATTTCGTGCCCGAAATTCCCGACCGCAGGATAGATATCCTATACCTCTGCTATCCCAATAACCC
TACGGGGACGGTCATTAGCAAGGCCGAACTGAAAAAATGGGTGAACTATGCATTGGAAAATGATACCCTCATCCTTTACG
ATGCTGCCTACGAGGCCTACATACAAGACCCGGACATTCCTCACTCCATTTATGAGATAAAAGGAGCGAAGAAAGTAGCG
ATAGAATTTCGCAGTTTCTCGAAGACGGCCGGATTCACCGGGGTGCGTTGCGGATACACCGTCGTACCGAAAGAACTCAC
AGCCGCCACGCTGGAAGGAGAGCGCATTCCGCTGAACCGGATGTGGAACCGCCGCCAGTGCACCAAGTTCAACGGGACAT
CCTATATCACCCAGCGTGGTGCCGAAGCGATCTATACCCCCGAGGGCAAAAAGCAAGTGAAAGCCATCATACAGTATTAT
ATGGCTAACGCCCGCATCATGAAAGAAGCGTTGGAAAGTACAGGACTCAAAGTTTTCGGCGGAGAGAACGCCCCCTACCT
GTGGGTGAAGGCGCCGGGCGAGGTAAGCTCGTGGAAATTCTTTGAACAGATGCTGTATGAAGCCAATGTAGTGGGCACGC
CGGGAGTAGGCTTCGGCCCCAGTGGTGAAGGATATATCCGCTTGACCGCTTTCGGAGAGCGGGCGGATTGCGAAGAAGCC
ATGAAACGGATCAGGAAATGGTTGCTCTGA

Upstream 100 bases:

>100_bases
GTATACATGACCGGTCCGGCCGGAAAGGTTTTCGAAGGCGAAATAGAGATGTGATACTGATTTTGAAAAACTTAAAACAA
CGATAAAAACAATAACAACG

Downstream 100 bases:

>100_bases
GCTGTACACGGCAACACCTTGAAGCACTGTGTGCTTGCACCGGACTTACATCAATCAAGCCAATAGGTATATAAAATATG
ACATACTCACTATTATTAAT

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MALVNEHFLKLPNNYLFSDIAKKVNAFKVSHPKTDLIRLGIGDVTRPLPQTSIEAMYKAVDELANKETFHGYGPEQGYDF
LIDAVIRNDYAPRGVYLEPGEVFISDGAKSDTGNIGDILRHDNSIGVTDPIYPVYIDSNVMCGRAGILEDGRWSNVVYLP
CLSENNFVPEIPDRRIDILYLCYPNNPTGTVISKAELKKWVNYALENDTLILYDAAYEAYIQDPDIPHSIYEIKGAKKVA
IEFRSFSKTAGFTGVRCGYTVVPKELTAATLEGERIPLNRMWNRRQCTKFNGTSYITQRGAEAIYTPEGKKQVKAIIQYY
MANARIMKEALESTGLKVFGGENAPYLWVKAPGEVSSWKFFEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGERADCEEA
MKRIRKWLL

Sequences:

>Translated_409_residues
MALVNEHFLKLPNNYLFSDIAKKVNAFKVSHPKTDLIRLGIGDVTRPLPQTSIEAMYKAVDELANKETFHGYGPEQGYDF
LIDAVIRNDYAPRGVYLEPGEVFISDGAKSDTGNIGDILRHDNSIGVTDPIYPVYIDSNVMCGRAGILEDGRWSNVVYLP
CLSENNFVPEIPDRRIDILYLCYPNNPTGTVISKAELKKWVNYALENDTLILYDAAYEAYIQDPDIPHSIYEIKGAKKVA
IEFRSFSKTAGFTGVRCGYTVVPKELTAATLEGERIPLNRMWNRRQCTKFNGTSYITQRGAEAIYTPEGKKQVKAIIQYY
MANARIMKEALESTGLKVFGGENAPYLWVKAPGEVSSWKFFEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGERADCEEA
MKRIRKWLL
>Mature_408_residues
ALVNEHFLKLPNNYLFSDIAKKVNAFKVSHPKTDLIRLGIGDVTRPLPQTSIEAMYKAVDELANKETFHGYGPEQGYDFL
IDAVIRNDYAPRGVYLEPGEVFISDGAKSDTGNIGDILRHDNSIGVTDPIYPVYIDSNVMCGRAGILEDGRWSNVVYLPC
LSENNFVPEIPDRRIDILYLCYPNNPTGTVISKAELKKWVNYALENDTLILYDAAYEAYIQDPDIPHSIYEIKGAKKVAI
EFRSFSKTAGFTGVRCGYTVVPKELTAATLEGERIPLNRMWNRRQCTKFNGTSYITQRGAEAIYTPEGKKQVKAIIQYYM
ANARIMKEALESTGLKVFGGENAPYLWVKAPGEVSSWKFFEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGERADCEEAM
KRIRKWLL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=384, Percent_Identity=25.5208333333333, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_BACV8 (A6L7E4)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301230.1
- ProteinModelPortal:   A6L7E4
- SMR:   A6L7E4
- STRING:   A6L7E4
- GeneID:   5304964
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4005
- NMPDR:   fig|435590.6.peg.3720
- eggNOG:   COG0436
- HOGENOM:   HBG645860
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 45917; Mature: 45786

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 186-186 BINDING 186-186 BINDING 214-214 BINDING 217-217 BINDING 245-245 BINDING 247-247 BINDING 256-256 BINDING 291-291 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVNEHFLKLPNNYLFSDIAKKVNAFKVSHPKTDLIRLGIGDVTRPLPQTSIEAMYKAV
CCCCCCHHHHCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCHHCCCCCHHHHHHHHHHH
DELANKETFHGYGPEQGYDFLIDAVIRNDYAPRGVYLEPGEVFISDGAKSDTGNIGDILR
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHH
HDNSIGVTDPIYPVYIDSNVMCGRAGILEDGRWSNVVYLPCLSENNFVPEIPDRRIDILY
CCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
LCYPNNPTGTVISKAELKKWVNYALENDTLILYDAAYEAYIQDPDIPHSIYEIKGAKKVA
EEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHCCCEEEE
IEFRSFSKTAGFTGVRCGYTVVPKELTAATLEGERIPLNRMWNRRQCTKFNGTSYITQRG
EEEHHCCCCCCCCEEECCCEECCCHHHHEECCCCCCCHHHHCCCCCCCCCCCCHHHHHCC
AEAIYTPEGKKQVKAIIQYYMANARIMKEALESTGLKVFGGENAPYLWVKAPGEVSSWKF
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHH
FEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGERADCEEAMKRIRKWLL
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVNEHFLKLPNNYLFSDIAKKVNAFKVSHPKTDLIRLGIGDVTRPLPQTSIEAMYKAV
CCCCCHHHHCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCHHCCCCCHHHHHHHHHHH
DELANKETFHGYGPEQGYDFLIDAVIRNDYAPRGVYLEPGEVFISDGAKSDTGNIGDILR
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHH
HDNSIGVTDPIYPVYIDSNVMCGRAGILEDGRWSNVVYLPCLSENNFVPEIPDRRIDILY
CCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
LCYPNNPTGTVISKAELKKWVNYALENDTLILYDAAYEAYIQDPDIPHSIYEIKGAKKVA
EEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCHHHHHCCCEEEE
IEFRSFSKTAGFTGVRCGYTVVPKELTAATLEGERIPLNRMWNRRQCTKFNGTSYITQRG
EEEHHCCCCCCCCEEECCCEECCCHHHHEECCCCCCCHHHHCCCCCCCCCCCCHHHHHCC
AEAIYTPEGKKQVKAIIQYYMANARIMKEALESTGLKVFGGENAPYLWVKAPGEVSSWKF
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHH
FEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGERADCEEAMKRIRKWLL
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA