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The map label for this gene is purF [H]

Identifier: 150006480

GI number: 150006480

Start: 4923230

End: 4925113

Strand: Reverse

Name: purF [H]

Synonym: BVU_3999

Alternate gene names: 150006480

Gene position: 4925113-4923230 (Counterclockwise)

Preceding gene: 150006481

Following gene: 150006479

Centisome position: 95.39

GC content: 47.13

Gene sequence:

>1884_bases
ATGGAACCGTTAAAGCATGAATGTGGAGTCGCTATGATACGACTGCTGAAACCGCTGGAATATTATCAGGAAAAGTATGG
AACCTGGATGTATGGCTTGAACAAGCTGTATCTGCTGATGGAAAAACAACACAACCGCGGACAGGAAGGCGCCGGGTTGG
CCTGTGTCAAACTGGAGGCCAATCCCGGTGAAGAATATATGTTCCGTGAAAGAGCTTTAGGTTCAGGGGCGATAACAGAG
ATATTCGGTACGGTGCAAAATCATTTCAAGGATTTGGCGCCTGAACAATTGCATGATGCCGGATACGCCAAGAAATGCCT
TCCCTTTGCCGGAGAGGTATATATGGGGCATTTGCGTTATTCCACTACCGGAAAAAGCGGTATCTCCTACGTGCATCCGT
TCTTGCGCCGTAACAACTGGCGTGCCAAGAATTTGGCGTTGTGCGGCAATTTCAACATGACCAATGTGGATGAGATTTTC
GCACGTATCACTGCCGACGGCCAGCATCCGCGTAAATATGCCGACACATATATTATGCTGGAACAAGTGGGGCACCGTTT
GGATCGCGAGGTGGAGCGCCTCTATGTGGAATGTGAGAAGGAAGGTCTGAAAGGCATGGATATTACTCACGCTATAGAAG
ACCGCATTGATCTGGGCAATGTCCTCAAGACTTCGAGCAAGGAGTGGGATGGAGGCTATGTGATCTGCGGTATGACCGGT
AGCGGTGAGTCTTTTGCGGTGCGCGATCCGTGGGGCATCCGTCCGGCTTTCTGGTACATGGACGATGAAATCATGGTATT
GGCATCCGAGCGCCCTGTCATTCAGACTGCCTTGAATGTATCTGCCGGAAGCATCAATGAATTGCAACCGGGACAGGCTA
TCCTTATAAGCAAGACTGGAAAGATGCGTCTGGCACAGATCAACCGGGCGAAAGAGAAGAAAGCCTGTTCCTTCGAACGT
ATCTATTTCAGCCGGGGAAGTGACATGGATATTTATAAAGAAAGAAAACAGCTGGGCGAAAAACTGGTGAATCCTATTTT
GAAAGCGGTAGACTATGATGTGGAGCATACCGTATTCTCTTTTATACCCAATACGGCGGAAGTGGCTTTCTATGGACTGC
TGGAAGGATTCGACAATTATCTGAATGAACTGAAAGTAAAGAAGATAGAGGCTTTGGGGCATCATCCCAATCACGAGGAA
CTGGAAAAAATCCTGTCCTGGCGTATCCGTAGCGAGAAAGTAGCCATCAAGGATATCAAGCTGCGTACCTTTATCGCCGA
AGGCAACAGTCGGAATGATTTGGCTGCCCATGTGTATGATATCACTTATGGAAGCCTTGTGCCTCATGTGGATAATCTGG
TGATTATTGATGACAGCATCGTCCGGGGAACTACGTTAAGACAGAGCATTATCGGCATCCTCGACCGCCTGCATCCGAAG
AAAATCGTCATTGTTTCTTCTTCGCCACAGGTGCGTTATCCTGATTATTATGGTATTGATATGGCCAGTATGGATCAGTT
TATCGCTTTCAAGGCTGCTATCGATTTATTGAAAGAACGGGATATGAAAGATGTGATAGCCCGTGCTTACAACAAATCGA
AAGACCAGGTGGGGTTACCCAAAGAGCAGATGGTGAATTATGTGAAAGAAATTTATGCCCCTTTCACTAATGAGGAGATT
GCGGCAAAAATGGTGGAACTTCTCACTCCGAAGGGTACGCAGGCGAAAGTGGAAATCGTTTATCAGACACTGGAAGGATT
GCATGAAGCGTGTCCCAACCATACGGGCGACTGGTATTTCAGTGGGGATTATCCTACTCCGGGTGGAGTGAAGCTGGTGA
ACCAGGCGTTTATAGACTATATAGAAAAAGTTTATCAATTCTAA

Upstream 100 bases:

>100_bases
CCGTAAGTTTGGAAAAATGACAGAAGGTCTTGTCATACCTTTGGGATGTTTTTATAAGAATCTGAAAATGAACTATTAAT
TTAAGTAGTAAAGAGAGAGC

Downstream 100 bases:

>100_bases
AGAACAACCATTCAAGATAGATTAGATGAGAAATGTAACATTGATCCTCGACGACGGGAGCCGTTTCCCGGGCAAGTCGT
TCGGTTATGAGAAACCGGTA

Product: putative phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MEPLKHECGVAMIRLLKPLEYYQEKYGTWMYGLNKLYLLMEKQHNRGQEGAGLACVKLEANPGEEYMFRERALGSGAITE
IFGTVQNHFKDLAPEQLHDAGYAKKCLPFAGEVYMGHLRYSTTGKSGISYVHPFLRRNNWRAKNLALCGNFNMTNVDEIF
ARITADGQHPRKYADTYIMLEQVGHRLDREVERLYVECEKEGLKGMDITHAIEDRIDLGNVLKTSSKEWDGGYVICGMTG
SGESFAVRDPWGIRPAFWYMDDEIMVLASERPVIQTALNVSAGSINELQPGQAILISKTGKMRLAQINRAKEKKACSFER
IYFSRGSDMDIYKERKQLGEKLVNPILKAVDYDVEHTVFSFIPNTAEVAFYGLLEGFDNYLNELKVKKIEALGHHPNHEE
LEKILSWRIRSEKVAIKDIKLRTFIAEGNSRNDLAAHVYDITYGSLVPHVDNLVIIDDSIVRGTTLRQSIIGILDRLHPK
KIVIVSSSPQVRYPDYYGIDMASMDQFIAFKAAIDLLKERDMKDVIARAYNKSKDQVGLPKEQMVNYVKEIYAPFTNEEI
AAKMVELLTPKGTQAKVEIVYQTLEGLHEACPNHTGDWYFSGDYPTPGGVKLVNQAFIDYIEKVYQF

Sequences:

>Translated_627_residues
MEPLKHECGVAMIRLLKPLEYYQEKYGTWMYGLNKLYLLMEKQHNRGQEGAGLACVKLEANPGEEYMFRERALGSGAITE
IFGTVQNHFKDLAPEQLHDAGYAKKCLPFAGEVYMGHLRYSTTGKSGISYVHPFLRRNNWRAKNLALCGNFNMTNVDEIF
ARITADGQHPRKYADTYIMLEQVGHRLDREVERLYVECEKEGLKGMDITHAIEDRIDLGNVLKTSSKEWDGGYVICGMTG
SGESFAVRDPWGIRPAFWYMDDEIMVLASERPVIQTALNVSAGSINELQPGQAILISKTGKMRLAQINRAKEKKACSFER
IYFSRGSDMDIYKERKQLGEKLVNPILKAVDYDVEHTVFSFIPNTAEVAFYGLLEGFDNYLNELKVKKIEALGHHPNHEE
LEKILSWRIRSEKVAIKDIKLRTFIAEGNSRNDLAAHVYDITYGSLVPHVDNLVIIDDSIVRGTTLRQSIIGILDRLHPK
KIVIVSSSPQVRYPDYYGIDMASMDQFIAFKAAIDLLKERDMKDVIARAYNKSKDQVGLPKEQMVNYVKEIYAPFTNEEI
AAKMVELLTPKGTQAKVEIVYQTLEGLHEACPNHTGDWYFSGDYPTPGGVKLVNQAFIDYIEKVYQF
>Mature_627_residues
MEPLKHECGVAMIRLLKPLEYYQEKYGTWMYGLNKLYLLMEKQHNRGQEGAGLACVKLEANPGEEYMFRERALGSGAITE
IFGTVQNHFKDLAPEQLHDAGYAKKCLPFAGEVYMGHLRYSTTGKSGISYVHPFLRRNNWRAKNLALCGNFNMTNVDEIF
ARITADGQHPRKYADTYIMLEQVGHRLDREVERLYVECEKEGLKGMDITHAIEDRIDLGNVLKTSSKEWDGGYVICGMTG
SGESFAVRDPWGIRPAFWYMDDEIMVLASERPVIQTALNVSAGSINELQPGQAILISKTGKMRLAQINRAKEKKACSFER
IYFSRGSDMDIYKERKQLGEKLVNPILKAVDYDVEHTVFSFIPNTAEVAFYGLLEGFDNYLNELKVKKIEALGHHPNHEE
LEKILSWRIRSEKVAIKDIKLRTFIAEGNSRNDLAAHVYDITYGSLVPHVDNLVIIDDSIVRGTTLRQSIIGILDRLHPK
KIVIVSSSPQVRYPDYYGIDMASMDQFIAFKAAIDLLKERDMKDVIARAYNKSKDQVGLPKEQMVNYVKEIYAPFTNEEI
AAKMVELLTPKGTQAKVEIVYQTLEGLHEACPNHTGDWYFSGDYPTPGGVKLVNQAFIDYIEKVYQF

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788651, Length=523, Percent_Identity=25.0478011472275, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323958, Length=415, Percent_Identity=25.3012048192771, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 71339; Mature: 71339

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLKHECGVAMIRLLKPLEYYQEKYGTWMYGLNKLYLLMEKQHNRGQEGAGLACVKLEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEHHCCCCCCCCCEEEEEEEC
NPGEEYMFRERALGSGAITEIFGTVQNHFKDLAPEQLHDAGYAKKCLPFAGEVYMGHLRY
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHCCCCCCEEEEEEEE
STTGKSGISYVHPFLRRNNWRAKNLALCGNFNMTNVDEIFARITADGQHPRKYADTYIML
ECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
EQVGHRLDREVERLYVECEKEGLKGMDITHAIEDRIDLGNVLKTSSKEWDGGYVICGMTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
SGESFAVRDPWGIRPAFWYMDDEIMVLASERPVIQTALNVSAGSINELQPGQAILISKTG
CCCEEEECCCCCCCCEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEECCC
KMRLAQINRAKEKKACSFERIYFSRGSDMDIYKERKQLGEKLVNPILKAVDYDVEHTVFS
CCHHHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FIPNTAEVAFYGLLEGFDNYLNELKVKKIEALGHHPNHEELEKILSWRIRSEKVAIKDIK
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEE
LRTFIAEGNSRNDLAAHVYDITYGSLVPHVDNLVIIDDSIVRGTTLRQSIIGILDRLHPK
EEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHCCCCC
KIVIVSSSPQVRYPDYYGIDMASMDQFIAFKAAIDLLKERDMKDVIARAYNKSKDQVGLP
EEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
KEQMVNYVKEIYAPFTNEEIAAKMVELLTPKGTQAKVEIVYQTLEGLHEACPNHTGDWYF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
SGDYPTPGGVKLVNQAFIDYIEKVYQF
ECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEPLKHECGVAMIRLLKPLEYYQEKYGTWMYGLNKLYLLMEKQHNRGQEGAGLACVKLEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEHHCCCCCCCCCEEEEEEEC
NPGEEYMFRERALGSGAITEIFGTVQNHFKDLAPEQLHDAGYAKKCLPFAGEVYMGHLRY
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHCCCCCCEEEEEEEE
STTGKSGISYVHPFLRRNNWRAKNLALCGNFNMTNVDEIFARITADGQHPRKYADTYIML
ECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
EQVGHRLDREVERLYVECEKEGLKGMDITHAIEDRIDLGNVLKTSSKEWDGGYVICGMTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
SGESFAVRDPWGIRPAFWYMDDEIMVLASERPVIQTALNVSAGSINELQPGQAILISKTG
CCCEEEECCCCCCCCEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEECCC
KMRLAQINRAKEKKACSFERIYFSRGSDMDIYKERKQLGEKLVNPILKAVDYDVEHTVFS
CCHHHHHHHHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FIPNTAEVAFYGLLEGFDNYLNELKVKKIEALGHHPNHEELEKILSWRIRSEKVAIKDIK
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEE
LRTFIAEGNSRNDLAAHVYDITYGSLVPHVDNLVIIDDSIVRGTTLRQSIIGILDRLHPK
EEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHCCCCC
KIVIVSSSPQVRYPDYYGIDMASMDQFIAFKAAIDLLKERDMKDVIARAYNKSKDQVGLP
EEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
KEQMVNYVKEIYAPFTNEEIAAKMVELLTPKGTQAKVEIVYQTLEGLHEACPNHTGDWYF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
SGDYPTPGGVKLVNQAFIDYIEKVYQF
ECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]