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The map label for this gene is gmd [H]

Identifier: 150006427

GI number: 150006427

Start: 4860230

End: 4861312

Strand: Reverse

Name: gmd [H]

Synonym: BVU_3946

Alternate gene names: 150006427

Gene position: 4861312-4860230 (Counterclockwise)

Preceding gene: 150006428

Following gene: 150006426

Centisome position: 94.15

GC content: 48.11

Gene sequence:

>1083_bases
ATGAAAAAAGTAGCTTTAATTTCAGGTATTACAGGTCAGGACGGTTCATTCCTGGCAGAGTTTTTGATTGAAAAGGGATA
TGAGGTTCACGGTATTCTGCGCCGTTCTTCTTCATTCAATACCGGTCGTATCGAACATCTTTATCTGGACGAGTGGGTTC
GTGATATGCAAAAGACCCGTCTGGTGAATCTGCATTGGGGGGATATGACCGACAGTAGTTCGCTGATACGTATCATTCAG
GCTGTACAGCCCGATGAAATCTATAATCTGGCTGCTCAGAGCCATGTGAAAGTAAGTTTCGATGTACCCGAGTACACAGC
GGAAGCGGATGCGGTAGGCACGCTGCGTATGTTGGAAGCGGTCCGTATTCTGGGCCTGGAAAAGAAGACCAAGATTTATC
AGGCATCGACTTCCGAATTGTTCGGACTGGTACAGGAAGTGCCTCAGAAGGAAACGACTCCCTTCTATCCCCGTTCTCCG
TATGGGGTGGCCAAGCAGTATGGTTTCTGGATTACCAAGAACTATCGTGAAAGTTACGGTATGTTTGCCGTGAACGGCAT
CCTGTTCAACCATGAGAGTGAACGCCGTGGTGAAAATTTCGTAACACGCAAGATTACTTTGGCTGCCGCACGTATAGCCC
AGGGATATCAGGACAAATTGTATCTGGGAAATCTGGATTCTTTGCGCGACTGGGGCTATGCCAAGGATTATGTGGAGTGT
ATGTGGATGATTCTTCAGCACGATACTCCCGAAGATTTTGTCATTGCGACCGGCGAATACCATACGGTGCGCGAGTTCTG
TACCCTGGCCTTCAAGGAAGTGGGCATTGAATTGCGCTGGGAAGGCAAAGGGGTGGATGAAAGAGGTATCGATATGGCGA
CGGGCAAGGAACTGGTTGCCGTCGATCCTAAGTATTTCCGTCCCGCCGAAGTGGAGCAGTTGTTGGGTGATCCCACTAAA
GCCAAAACGTTGCTGGGCTGGAATCCCCGTAAGACTTCTTTCCCCGAACTGGTGAAAATCATGGTGAAGCATGATATGAA
GTTCGTGAAGAAACTGTATTTAAAGGCTCAAATAGCAGAATAA

Upstream 100 bases:

>100_bases
CATTCCGCCCTTTGCCATGCCGAAAGGCAGATAGCAGTAGATAGCTATGTGATATGAAATAACCAAGAGGGCTTGCATAA
GGCCATTTACAATAAACGAA

Downstream 100 bases:

>100_bases
TGGAAAAAACAGCCAAGATATATGTAGCAGGCCACCACGGACTGGTGGGGTCTGCTATATGGAATAATTTGCAGCAAAAG
GGATATACCAATCTGGTAGG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMQKTRLVNLHWGDMTDSSSLIRIIQ
AVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGENFVTRKITLAAARIAQGYQDKLYLGNLDSLRDWGYAKDYVEC
MWMILQHDTPEDFVIATGEYHTVREFCTLAFKEVGIELRWEGKGVDERGIDMATGKELVAVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPRKTSFPELVKIMVKHDMKFVKKLYLKAQIAE

Sequences:

>Translated_360_residues
MKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMQKTRLVNLHWGDMTDSSSLIRIIQ
AVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGENFVTRKITLAAARIAQGYQDKLYLGNLDSLRDWGYAKDYVEC
MWMILQHDTPEDFVIATGEYHTVREFCTLAFKEVGIELRWEGKGVDERGIDMATGKELVAVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPRKTSFPELVKIMVKHDMKFVKKLYLKAQIAE
>Mature_360_residues
MKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMQKTRLVNLHWGDMTDSSSLIRIIQ
AVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGENFVTRKITLAAARIAQGYQDKLYLGNLDSLRDWGYAKDYVEC
MWMILQHDTPEDFVIATGEYHTVREFCTLAFKEVGIELRWEGKGVDERGIDMATGKELVAVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPRKTSFPELVKIMVKHDMKFVKKLYLKAQIAE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=62.1776504297994, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=63.98891966759, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI48994969, Length=185, Percent_Identity=29.7297297297297, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788353, Length=256, Percent_Identity=25.78125, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=63.8968481375358, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539424, Length=349, Percent_Identity=63.0372492836676, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901790, Length=349, Percent_Identity=63.0372492836676, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901788, Length=349, Percent_Identity=63.0372492836676, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539422, Length=349, Percent_Identity=63.0372492836676, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901786, Length=349, Percent_Identity=63.0372492836676, Blast_Score=459, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=63.323782234957, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41249; Mature: 41249

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMQKTR
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHCE
LVNLHWGDMTDSSSLIRIIQAVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEA
EEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHH
VRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESYG
HHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHCC
MFAVNGILFNHESERRGENFVTRKITLAAARIAQGYQDKLYLGNLDSLRDWGYAKDYVEC
EEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHH
MWMILQHDTPEDFVIATGEYHTVREFCTLAFKEVGIELRWEGKGVDERGIDMATGKELVA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEE
VDPKYFRPAEVEQLLGDPTKAKTLLGWNPRKTSFPELVKIMVKHDMKFVKKLYLKAQIAE
ECCCCCCHHHHHHHHCCCCHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMQKTR
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHCE
LVNLHWGDMTDSSSLIRIIQAVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEA
EEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHH
VRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESYG
HHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHCC
MFAVNGILFNHESERRGENFVTRKITLAAARIAQGYQDKLYLGNLDSLRDWGYAKDYVEC
EEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHH
MWMILQHDTPEDFVIATGEYHTVREFCTLAFKEVGIELRWEGKGVDERGIDMATGKELVA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEE
VDPKYFRPAEVEQLLGDPTKAKTLLGWNPRKTSFPELVKIMVKHDMKFVKKLYLKAQIAE
ECCCCCCHHHHHHHHCCCCHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]