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The map label for this gene is hisF

Identifier: 150006334

GI number: 150006334

Start: 4753695

End: 4754450

Strand: Direct

Name: hisF

Synonym: BVU_3853

Alternate gene names: 150006334

Gene position: 4753695-4754450 (Clockwise)

Preceding gene: 150006333

Following gene: 150006335

Centisome position: 92.07

GC content: 47.09

Gene sequence:

>756_bases
ATGTTAGCAAAAAGAATCATACCTTGCCTGGACATCAAAGACGGGCAGACCGTAAAAGGAACCAATTTCGTAAACTTACG
TCAAGCAGGTGATCCGGTAGAACTGGGGCGTACCTACAGTGAACAGGGAGCTGACGAACTGGTCTTTCTCGATATCACTG
CCAGCCATGAAGGGCGCAAAACCTTTACCGATTTGGTAAAACGGGTAGCGGCCAATATCAATATCCCCTTTACTGTAGGA
GGAGGTATAAACGAACTGAGCGACGTAGACCGGCTATTGAATGCAGGAGCTGACAAAGTTTCTATCAATTCATCAGCCAT
CCGCAATCCGGATTTGGTAGACAAGATTGCCAAACATTTCGGTTCACAGGTCTGTGTGGTAGCCATTGACGCCAAGCAGA
CTGAAACCGGGTGGAAATGCTATCTGAACGGCGGCCGGATTGAAACCGACAAGTACCTGTTTGACTGGGCTAAAGAGGTG
AACAACCGCGGGGCAGGAGAAATTCTTTTCACGAGCATGAACCATGACGGAGTGAAAAACGGATATGCCAACGAAGCCCT
CTCCACCCTGGCCGATTTACTGACAATTCCCGTCATCGCATCTGGAGGCGCCGGATGCATGGAACATTTCCGCGATACAT
TTGCAAAAGGGAAAGCAGATGCTGCATTAGCAGCCAGCGTTTTTCATTTCGGAGAAATTAAAATTCCCGAATTAAAACAA
TATCTTTGCGAACAAGGAATTAATGTGAGGTTATAA

Upstream 100 bases:

>100_bases
CCTGGAAATAGCCAAAGTTCCGGCTGTTGTTTTCGGCAAGGCATTATACGAGGGAAAAATTACATTGAAAGACTTAAACC
GTTTCATGTAAAAGCAAATC

Downstream 100 bases:

>100_bases
CTCTGTTTTTAATTGCATCTAAATCTGTTTCCACTACCATCCCAAACAGGATGAAAGACTGGCAAACGACCTTCAGATCC
TCATTCCACTCTGAATGACG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRTYSEQGADELVFLDITASHEGRKTFTDLVKRVAANINIPFTVG
GGINELSDVDRLLNAGADKVSINSSAIRNPDLVDKIAKHFGSQVCVVAIDAKQTETGWKCYLNGGRIETDKYLFDWAKEV
NNRGAGEILFTSMNHDGVKNGYANEALSTLADLLTIPVIASGGAGCMEHFRDTFAKGKADAALAASVFHFGEIKIPELKQ
YLCEQGINVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRTYSEQGADELVFLDITASHEGRKTFTDLVKRVAANINIPFTVG
GGINELSDVDRLLNAGADKVSINSSAIRNPDLVDKIAKHFGSQVCVVAIDAKQTETGWKCYLNGGRIETDKYLFDWAKEV
NNRGAGEILFTSMNHDGVKNGYANEALSTLADLLTIPVIASGGAGCMEHFRDTFAKGKADAALAASVFHFGEIKIPELKQ
YLCEQGINVRL
>Mature_251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRTYSEQGADELVFLDITASHEGRKTFTDLVKRVAANINIPFTVG
GGINELSDVDRLLNAGADKVSINSSAIRNPDLVDKIAKHFGSQVCVVAIDAKQTETGWKCYLNGGRIETDKYLFDWAKEV
NNRGAGEILFTSMNHDGVKNGYANEALSTLADLLTIPVIASGGAGCMEHFRDTFAKGKADAALAASVFHFGEIKIPELKQ
YLCEQGINVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=50.7751937984496, Blast_Score=243, Evalue=8e-66,
Organism=Escherichia coli, GI87082028, Length=180, Percent_Identity=31.6666666666667, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=32.1656050955414, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BACV8 (A6L6Z2)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301078.1
- ProteinModelPortal:   A6L6Z2
- SMR:   A6L6Z2
- STRING:   A6L6Z2
- GeneID:   5304812
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3853
- NMPDR:   fig|435590.6.peg.3570
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27218; Mature: 27218

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRTYSEQGADELVFLDITASHEGRK
CCHHHCCCCEECCCCCEECCCCEEEECCCCCHHHHCCCHHHCCCCCEEEEEEECCCCCHH
TFTDLVKRVAANINIPFTVGGGINELSDVDRLLNAGADKVSINSSAIRNPDLVDKIAKHF
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHC
GSQVCVVAIDAKQTETGWKCYLNGGRIETDKYLFDWAKEVNNRGAGEILFTSMNHDGVKN
CCCEEEEEEECCCCCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
GYANEALSTLADLLTIPVIASGGAGCMEHFRDTFAKGKADAALAASVFHFGEIKIPELKQ
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
YLCEQGINVRL
HHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRTYSEQGADELVFLDITASHEGRK
CCHHHCCCCEECCCCCEECCCCEEEECCCCCHHHHCCCHHHCCCCCEEEEEEECCCCCHH
TFTDLVKRVAANINIPFTVGGGINELSDVDRLLNAGADKVSINSSAIRNPDLVDKIAKHF
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHC
GSQVCVVAIDAKQTETGWKCYLNGGRIETDKYLFDWAKEVNNRGAGEILFTSMNHDGVKN
CCCEEEEEEECCCCCCCCEEEECCCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
GYANEALSTLADLLTIPVIASGGAGCMEHFRDTFAKGKADAALAASVFHFGEIKIPELKQ
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
YLCEQGINVRL
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA