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The map label for this gene is ygiC [H]

Identifier: 150006044

GI number: 150006044

Start: 4401407

End: 4402531

Strand: Reverse

Name: ygiC [H]

Synonym: BVU_3548

Alternate gene names: 150006044

Gene position: 4402531-4401407 (Counterclockwise)

Preceding gene: 150006045

Following gene: 150006034

Centisome position: 85.27

GC content: 44.0

Gene sequence:

>1125_bases
ATGAAAAGAATACCTGTTACGCCACGTTCAGACTACCGTGAGAAAATAGAAGCGCTGGGCTTTGATTTTCATGGTGATTA
TTGGCGCGAGGAAGCGTATTATCGTTTTACGGCTGAAGAAACAGCCTGTTTGGAGGAAGCAACCAATGAGGCGTACCGTC
TGTATTGTGAGGCTGCTCAATTCATTATTGAAGATAACCCGGAATTTATGGAGCGTGCATTGAATATTCCGAAGGAGATT
GGTGAGCGTATCCGTGCTTCTTGGGATGCGGATGAGTTAAGTCTGTATGGTCGTTTTGATTTTATATTAGCCAAGGATGG
TACTCCGAAAATATTGGAGTTCAATGCTGATACACCGACTTCTTTGCTTGAAGCATCTGTTATCCAATGGCAATGGAAAG
AGGATGTTTTTCCGGAATGCGACCAGTTTAATGGTATTCATGAAGGATTGGTGCAGTCATGGAAAGATATTTTCCCCAAA
AAAGGAGAGATTCATTTTGCTGGTGCATTAGAGAATAATGAAGATACAGGAACGTTGCAATATCTGGCAAGTACGGCGAT
GGAGGCTGGGTTTTCTACCCGAGTATTGGATATGCAGGCGCTGGATTTGCAAAATGGACGATTTTTTGATCCGGCAGGTG
AACTAGTGAACCGGTGCTTTAAACTTTATCCGTGGGAGTGGATGGTGGACGAAAGTCCTGACGGATGTCTAGCGGAAATG
GAATGGTTGGAGCCGATATGGAAATTGGTGATGTCCAATAAGGCGATCCTTGCCACATTGTACGAGTTGTTTCCGGATTC
TCCTTATGTGCTTCCTTGTTATTTGTCGCGCCCTCGGTCGGGATTGTTTTGTAAAAAACCGGTCTTCTCTCGTGAAGGTC
ATAATGTAAGCGTTGTTGATATCCGGGATTGGGAGGAATATGTACGTATAGCTGAAACGGAGGGTGATTATAACACTGGA
GCTTATGTATATCAGGAGTATGTGGAACCGACAGCGTACAGTGGCAGATATCCAGTTATTGGTTCTTGGATTATAGGAGG
AGAAGCTGCGGGCATCGGTATTCGCGAAAATCGTATGGAGGTTACAGATAACTTGTCCGAGTTTGTACCACATATTATAG
GATAA

Upstream 100 bases:

>100_bases
GAATGTGGGGGCAAAATCGTCTACAAAATCAAAATCGAGCGGAAGTGTTTTTGGGAAAACACGCCGTTCATCCGGTGTTC
ATAGTTAATAGGAAGCGGAT

Downstream 100 bases:

>100_bases
AGTCAAACTATCCATGTGCCGGTCGATGCCGGACGACGGATGTCGTCATGCCTCGACTGTTGGCACATAATTGAGGAATT
AATGTCTTTTGAATAGGGTC

Product: glutathionylspermidine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKRIPVTPRSDYREKIEALGFDFHGDYWREEAYYRFTAEETACLEEATNEAYRLYCEAAQFIIEDNPEFMERALNIPKEI
GERIRASWDADELSLYGRFDFILAKDGTPKILEFNADTPTSLLEASVIQWQWKEDVFPECDQFNGIHEGLVQSWKDIFPK
KGEIHFAGALENNEDTGTLQYLASTAMEAGFSTRVLDMQALDLQNGRFFDPAGELVNRCFKLYPWEWMVDESPDGCLAEM
EWLEPIWKLVMSNKAILATLYELFPDSPYVLPCYLSRPRSGLFCKKPVFSREGHNVSVVDIRDWEEYVRIAETEGDYNTG
AYVYQEYVEPTAYSGRYPVIGSWIIGGEAAGIGIRENRMEVTDNLSEFVPHIIG

Sequences:

>Translated_374_residues
MKRIPVTPRSDYREKIEALGFDFHGDYWREEAYYRFTAEETACLEEATNEAYRLYCEAAQFIIEDNPEFMERALNIPKEI
GERIRASWDADELSLYGRFDFILAKDGTPKILEFNADTPTSLLEASVIQWQWKEDVFPECDQFNGIHEGLVQSWKDIFPK
KGEIHFAGALENNEDTGTLQYLASTAMEAGFSTRVLDMQALDLQNGRFFDPAGELVNRCFKLYPWEWMVDESPDGCLAEM
EWLEPIWKLVMSNKAILATLYELFPDSPYVLPCYLSRPRSGLFCKKPVFSREGHNVSVVDIRDWEEYVRIAETEGDYNTG
AYVYQEYVEPTAYSGRYPVIGSWIIGGEAAGIGIRENRMEVTDNLSEFVPHIIG
>Mature_374_residues
MKRIPVTPRSDYREKIEALGFDFHGDYWREEAYYRFTAEETACLEEATNEAYRLYCEAAQFIIEDNPEFMERALNIPKEI
GERIRASWDADELSLYGRFDFILAKDGTPKILEFNADTPTSLLEASVIQWQWKEDVFPECDQFNGIHEGLVQSWKDIFPK
KGEIHFAGALENNEDTGTLQYLASTAMEAGFSTRVLDMQALDLQNGRFFDPAGELVNRCFKLYPWEWMVDESPDGCLAEM
EWLEPIWKLVMSNKAILATLYELFPDSPYVLPCYLSRPRSGLFCKKPVFSREGHNVSVVDIRDWEEYVRIAETEGDYNTG
AYVYQEYVEPTAYSGRYPVIGSWIIGGEAAGIGIRENRMEVTDNLSEFVPHIIG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli yjfC [H]

Homologues:

Organism=Escherichia coli, GI1789416, Length=390, Percent_Identity=38.4615384615385, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1790629, Length=393, Percent_Identity=33.8422391857506, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1789361, Length=401, Percent_Identity=25.6857855361596, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 43088; Mature: 43088

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIPVTPRSDYREKIEALGFDFHGDYWREEAYYRFTAEETACLEEATNEAYRLYCEAAQ
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
FIIEDNPEFMERALNIPKEIGERIRASWDADELSLYGRFDFILAKDGTPKILEFNADTPT
HHHCCCHHHHHHHHCCHHHHHHHHHCCCCHHHEEEEEEEEEEEECCCCCCEEEECCCCCH
SLLEASVIQWQWKEDVFPECDQFNGIHEGLVQSWKDIFPKKGEIHFAGALENNEDTGTLQ
HHHHHHHHHEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YLASTAMEAGFSTRVLDMQALDLQNGRFFDPAGELVNRCFKLYPWEWMVDESPDGCLAEM
HHHHHHHHCCCCEEEEEEEEEECCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
EWLEPIWKLVMSNKAILATLYELFPDSPYVLPCYLSRPRSGLFCKKPVFSREGHNVSVVD
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCEEEEEE
IRDWEEYVRIAETEGDYNTGAYVYQEYVEPTAYSGRYPVIGSWIIGGEAAGIGIRENRME
ECCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHH
VTDNLSEFVPHIIG
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIPVTPRSDYREKIEALGFDFHGDYWREEAYYRFTAEETACLEEATNEAYRLYCEAAQ
CCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
FIIEDNPEFMERALNIPKEIGERIRASWDADELSLYGRFDFILAKDGTPKILEFNADTPT
HHHCCCHHHHHHHHCCHHHHHHHHHCCCCHHHEEEEEEEEEEEECCCCCCEEEECCCCCH
SLLEASVIQWQWKEDVFPECDQFNGIHEGLVQSWKDIFPKKGEIHFAGALENNEDTGTLQ
HHHHHHHHHEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
YLASTAMEAGFSTRVLDMQALDLQNGRFFDPAGELVNRCFKLYPWEWMVDESPDGCLAEM
HHHHHHHHCCCCEEEEEEEEEECCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
EWLEPIWKLVMSNKAILATLYELFPDSPYVLPCYLSRPRSGLFCKKPVFSREGHNVSVVD
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCEEEEEE
IRDWEEYVRIAETEGDYNTGAYVYQEYVEPTAYSGRYPVIGSWIIGGEAAGIGIRENRME
ECCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHH
VTDNLSEFVPHIIG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]