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The map label for this gene is pcrA [H]

Identifier: 150006034

GI number: 150006034

Start: 4391379

End: 4393769

Strand: Reverse

Name: pcrA [H]

Synonym: BVU_3538

Alternate gene names: 150006034

Gene position: 4393769-4391379 (Counterclockwise)

Preceding gene: 150006044

Following gene: 150006033

Centisome position: 85.1

GC content: 44.21

Gene sequence:

>2391_bases
ATGACGAACTATTTAGAAGAATTGAATGAAAGTCAGCGCAATGCGGTGCTTTACAACGAAGGTCCCTCTTTGGTGATAGC
CGGAGCAGGTTCGGGGAAGACACGCGTACTGACTTATAAAATAGCTTATTTACTGGAGCGGGGATATACTCCTTGGTCTA
TTCTGGCATTGACTTTTACCAATAAGGCTGCTCGTGAAATGAAAGAGCGTATAGCCCGTCAGGTGGGCGATCAGGCACGC
TATTTATGGATGGGAACGTTCCATTCCATTTTTTCGCGTATCCTGCGGTGTGAGGCACAGGCTGTCGGCTTTACCTCCAA
TTTTACCATCTATGATTCTTCAGATTCGAAGAGTTTGATCAAGTCTATTGTAAAAGAAATGCAATTGGATGATAAAACCT
ATAAACCGGGGGTGATACAGGCGCGTATCAGCAATGCGAAAAACCATTTGGTTTTTCCCGATGCATATGTTTCGAACGCA
GAGCTTTATCAGTATGATCTGAATGCCAAAGTACCCGCTACCCGTGATATTTACCGACGATATTGGGAACGTTGTCGCCA
AAGCGATGCGATGGATTTTGATGATTTGTTGGTTTATACTTATATGTTATTCCGCGATCATCCCGAAATCTGCCGTAAGT
ATGCGGAGCAGTTCCGTTATGTGCTGGTGGATGAGTATCAGGATACTAATTTTGCGCAACACAGCATTGTGCTTCAGCTT
ACCCAAGAACATCAGCGTGTATGCGTAGTGGGGGATGATGCACAAAGTATTTATTCCTTCCGAGGGGCGAATATTGACAA
TATATTGAAATTTACTCAGCTGTATAAGGGAGCCAAGCTTTTTAAATTGGAGCAGAACTATCGTTCTACCCAGACCATTG
TATGCGCGGCAAACAGTTTGATAGAGAAAAATAGCGAGCAGATAAGGAAGGAGGTATTTTCGGAAAAAGCGAAAGGGGAG
CCTATCGGAGTTTTCAATGCCTACAGTGATGTGGAGGAAGGGGAGATTGTTGCCAACCAGATTGTGAAACTGCGTTCGCG
GGAACATTATTCTTATAATGATTTTGCTATTTTATACCGTACAAATGCTCAGAGCCGTATTTTTGAGGAGGCTTTGCGGA
AGCGTGCTTTACCTTATAAGATTTACGGAGGGCTTTCTTTTTATCAACGCAAGGAGATTAAAGATGTTATATCTTACTTC
CGTCTGGCGGTAAATCCGAATGATGAGGAGGCTTTTAAGCGGGTGTTGAATTATCCGGCGCGAGGAATCGGGGATACTAC
GTTGAACAAGGTCATTGATGCGGCTGCACAGCATCATGTCAGTCTTTGGATGGTTTTGGAAGAGCCGTTGGCATACGGCT
TGAATATTAACAAGGGTATGCATACTAAATTACAGGGGTTTCGTGAACTGGTAGAAAGTTTTATAGTGGAGGTCCCGAAG
AAGAATGCATACGAATTGGGAGCAATGATTGTTCGTCAGTCCGGCATTATGAATGAGATTTATCAGAGCAATGATCCTGA
AAATCTGAGCCGTCAGGAGAATATTGAAGAACTGATAAACGGTATGCACGATTTTTGTGCAACCCGTGAAGAAGAAGGGA
ATGAAAACATCCTGTTGACGGATTTTCTGTCGGAGGTTTCTCTGTTGACAGACCAGGATAACGAGAAAGAGGGGGATGCC
GAGAAGATTACCTTGATGACCATTCACTCTGCTAAAGGCTTGGAGTTTAAAAACGTCTTTGTAGTTGGGATGGAAGAGAA
CTTGTTTCCCAGTGCTTTGTCACTCAATTCGTATAAGGAGCTGGAAGAGGAGCGCCGCTTGTTTTATGTGGCGATTACAC
GTGCCGAAGAGCATTGCTATTTGTCTTTTGCCAAGAGTCGGTTTAAATATGGAAAGATGGAGTTCAGTAGCCCTAGCCGT
TTCTTGAAAGATATTGATGTACATTTCCTGAAGTTACCTCAGGAGGAACAGATGGCCCGGAGAATAGACGAGAGAGCCAG
CCGTTTCTGTCGTGAGCAAAATGAACGTGCGGCTCGTTCCTTGTTTGACGAACCTGCTTCTCAGTCTTCTTACGGGCGTT
CTTCATCGAACATCTCTGGTGGTCAGAAAACATTTTTAGTTGGAGAAAGACCGAAAGTTGAGATTATTCGTCCCAGTATA
CCACGTAATTTGACCAAGGTTGGTCCGGAAACGGTCTCAAAGCCGTCCGCAGCGAGAAACTTGTCAGTGAATGTACAGGC
AGGGCAGGTGATAGAGCATGAGCGTTTTGGAATAGGCGATGTGATAAAGGTGGAAGGAATTGGTGAGAACAGCAAAGCGA
CTGTCCGTTTCCGGAATGCCGGAGAAAAACAATTATTACTGAAATTTGCCCGATTCAAAGTTGTGGAATAG

Upstream 100 bases:

>100_bases
GAGGTTTTAATTAAACTTCTATCATAATAACATTATTGAGCATAGAATTGTTCTGATAAACTCAAACTTTCTCTATCTTT
GCGGGCGAAAGGTGAAATTT

Downstream 100 bases:

>100_bases
AATAAATATTTTAATAGAATTCTGATACAAAAATGATAGATATCCATAAAGTACCTTCTCCATGCTATGTAATGGATGAA
GCATTGCTCAGAAAGAATCT

Product: putative helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MTNYLEELNESQRNAVLYNEGPSLVIAGAGSGKTRVLTYKIAYLLERGYTPWSILALTFTNKAAREMKERIARQVGDQAR
YLWMGTFHSIFSRILRCEAQAVGFTSNFTIYDSSDSKSLIKSIVKEMQLDDKTYKPGVIQARISNAKNHLVFPDAYVSNA
ELYQYDLNAKVPATRDIYRRYWERCRQSDAMDFDDLLVYTYMLFRDHPEICRKYAEQFRYVLVDEYQDTNFAQHSIVLQL
TQEHQRVCVVGDDAQSIYSFRGANIDNILKFTQLYKGAKLFKLEQNYRSTQTIVCAANSLIEKNSEQIRKEVFSEKAKGE
PIGVFNAYSDVEEGEIVANQIVKLRSREHYSYNDFAILYRTNAQSRIFEEALRKRALPYKIYGGLSFYQRKEIKDVISYF
RLAVNPNDEEAFKRVLNYPARGIGDTTLNKVIDAAAQHHVSLWMVLEEPLAYGLNINKGMHTKLQGFRELVESFIVEVPK
KNAYELGAMIVRQSGIMNEIYQSNDPENLSRQENIEELINGMHDFCATREEEGNENILLTDFLSEVSLLTDQDNEKEGDA
EKITLMTIHSAKGLEFKNVFVVGMEENLFPSALSLNSYKELEEERRLFYVAITRAEEHCYLSFAKSRFKYGKMEFSSPSR
FLKDIDVHFLKLPQEEQMARRIDERASRFCREQNERAARSLFDEPASQSSYGRSSSNISGGQKTFLVGERPKVEIIRPSI
PRNLTKVGPETVSKPSAARNLSVNVQAGQVIEHERFGIGDVIKVEGIGENSKATVRFRNAGEKQLLLKFARFKVVE

Sequences:

>Translated_796_residues
MTNYLEELNESQRNAVLYNEGPSLVIAGAGSGKTRVLTYKIAYLLERGYTPWSILALTFTNKAAREMKERIARQVGDQAR
YLWMGTFHSIFSRILRCEAQAVGFTSNFTIYDSSDSKSLIKSIVKEMQLDDKTYKPGVIQARISNAKNHLVFPDAYVSNA
ELYQYDLNAKVPATRDIYRRYWERCRQSDAMDFDDLLVYTYMLFRDHPEICRKYAEQFRYVLVDEYQDTNFAQHSIVLQL
TQEHQRVCVVGDDAQSIYSFRGANIDNILKFTQLYKGAKLFKLEQNYRSTQTIVCAANSLIEKNSEQIRKEVFSEKAKGE
PIGVFNAYSDVEEGEIVANQIVKLRSREHYSYNDFAILYRTNAQSRIFEEALRKRALPYKIYGGLSFYQRKEIKDVISYF
RLAVNPNDEEAFKRVLNYPARGIGDTTLNKVIDAAAQHHVSLWMVLEEPLAYGLNINKGMHTKLQGFRELVESFIVEVPK
KNAYELGAMIVRQSGIMNEIYQSNDPENLSRQENIEELINGMHDFCATREEEGNENILLTDFLSEVSLLTDQDNEKEGDA
EKITLMTIHSAKGLEFKNVFVVGMEENLFPSALSLNSYKELEEERRLFYVAITRAEEHCYLSFAKSRFKYGKMEFSSPSR
FLKDIDVHFLKLPQEEQMARRIDERASRFCREQNERAARSLFDEPASQSSYGRSSSNISGGQKTFLVGERPKVEIIRPSI
PRNLTKVGPETVSKPSAARNLSVNVQAGQVIEHERFGIGDVIKVEGIGENSKATVRFRNAGEKQLLLKFARFKVVE
>Mature_795_residues
TNYLEELNESQRNAVLYNEGPSLVIAGAGSGKTRVLTYKIAYLLERGYTPWSILALTFTNKAAREMKERIARQVGDQARY
LWMGTFHSIFSRILRCEAQAVGFTSNFTIYDSSDSKSLIKSIVKEMQLDDKTYKPGVIQARISNAKNHLVFPDAYVSNAE
LYQYDLNAKVPATRDIYRRYWERCRQSDAMDFDDLLVYTYMLFRDHPEICRKYAEQFRYVLVDEYQDTNFAQHSIVLQLT
QEHQRVCVVGDDAQSIYSFRGANIDNILKFTQLYKGAKLFKLEQNYRSTQTIVCAANSLIEKNSEQIRKEVFSEKAKGEP
IGVFNAYSDVEEGEIVANQIVKLRSREHYSYNDFAILYRTNAQSRIFEEALRKRALPYKIYGGLSFYQRKEIKDVISYFR
LAVNPNDEEAFKRVLNYPARGIGDTTLNKVIDAAAQHHVSLWMVLEEPLAYGLNINKGMHTKLQGFRELVESFIVEVPKK
NAYELGAMIVRQSGIMNEIYQSNDPENLSRQENIEELINGMHDFCATREEEGNENILLTDFLSEVSLLTDQDNEKEGDAE
KITLMTIHSAKGLEFKNVFVVGMEENLFPSALSLNSYKELEEERRLFYVAITRAEEHCYLSFAKSRFKYGKMEFSSPSRF
LKDIDVHFLKLPQEEQMARRIDERASRFCREQNERAARSLFDEPASQSSYGRSSSNISGGQKTFLVGERPKVEIIRPSIP
RNLTKVGPETVSKPSAARNLSVNVQAGQVIEHERFGIGDVIKVEGIGENSKATVRFRNAGEKQLLLKFARFKVVE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=794, Percent_Identity=36.0201511335013, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI48994965, Length=659, Percent_Identity=36.1153262518968, Blast_Score=381, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322369, Length=740, Percent_Identity=28.9189189189189, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324477, Length=667, Percent_Identity=23.3883058470765, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 91469; Mature: 91338

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYLEELNESQRNAVLYNEGPSLVIAGAGSGKTRVLTYKIAYLLERGYTPWSILALTFT
CCCHHHHHCCCCCCEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHCCCCCCEEEEEEEC
NKAAREMKERIARQVGDQARYLWMGTFHSIFSRILRCEAQAVGFTSNFTIYDSSDSKSLI
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
KSIVKEMQLDDKTYKPGVIQARISNAKNHLVFPDAYVSNAELYQYDLNAKVPATRDIYRR
HHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHHH
YWERCRQSDAMDFDDLLVYTYMLFRDHPEICRKYAEQFRYVLVDEYQDTNFAQHSIVLQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHEEE
TQEHQRVCVVGDDAQSIYSFRGANIDNILKFTQLYKGAKLFKLEQNYRSTQTIVCAANSL
ECCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEEHHHH
IEKNSEQIRKEVFSEKAKGEPIGVFNAYSDVEEGEIVANQIVKLRSREHYSYNDFAILYR
HHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
TNAQSRIFEEALRKRALPYKIYGGLSFYQRKEIKDVISYFRLAVNPNDEEAFKRVLNYPA
CCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCC
RGIGDTTLNKVIDAAAQHHVSLWMVLEEPLAYGLNINKGMHTKLQGFRELVESFIVEVPK
CCCCHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
KNAYELGAMIVRQSGIMNEIYQSNDPENLSRQENIEELINGMHDFCATREEEGNENILLT
CCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHH
DFLSEVSLLTDQDNEKEGDAEKITLMTIHSAKGLEFKNVFVVGMEENLFPSALSLNSYKE
HHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEECCEEEEEECCCCCCCHHCCHHHHH
LEEERRLFYVAITRAEEHCYLSFAKSRFKYGKMEFSSPSRFLKDIDVHFLKLPQEEQMAR
HHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHEECCCHHHHHH
RIDERASRFCREQNERAARSLFDEPASQSSYGRSSSNISGGQKTFLVGERPKVEIIRPSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCC
PRNLTKVGPETVSKPSAARNLSVNVQAGQVIEHERFGIGDVIKVEGIGENSKATVRFRNA
CCHHHHCCCHHCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEECCCCCCEEEEECCC
GEKQLLLKFARFKVVE
CHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNYLEELNESQRNAVLYNEGPSLVIAGAGSGKTRVLTYKIAYLLERGYTPWSILALTFT
CCHHHHHCCCCCCEEEEECCCCEEEEECCCCCEEEEHHHHHHHHHCCCCCCEEEEEEEC
NKAAREMKERIARQVGDQARYLWMGTFHSIFSRILRCEAQAVGFTSNFTIYDSSDSKSLI
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
KSIVKEMQLDDKTYKPGVIQARISNAKNHLVFPDAYVSNAELYQYDLNAKVPATRDIYRR
HHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHHH
YWERCRQSDAMDFDDLLVYTYMLFRDHPEICRKYAEQFRYVLVDEYQDTNFAQHSIVLQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHEEE
TQEHQRVCVVGDDAQSIYSFRGANIDNILKFTQLYKGAKLFKLEQNYRSTQTIVCAANSL
ECCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEEHHHH
IEKNSEQIRKEVFSEKAKGEPIGVFNAYSDVEEGEIVANQIVKLRSREHYSYNDFAILYR
HHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
TNAQSRIFEEALRKRALPYKIYGGLSFYQRKEIKDVISYFRLAVNPNDEEAFKRVLNYPA
CCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCC
RGIGDTTLNKVIDAAAQHHVSLWMVLEEPLAYGLNINKGMHTKLQGFRELVESFIVEVPK
CCCCHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
KNAYELGAMIVRQSGIMNEIYQSNDPENLSRQENIEELINGMHDFCATREEEGNENILLT
CCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHH
DFLSEVSLLTDQDNEKEGDAEKITLMTIHSAKGLEFKNVFVVGMEENLFPSALSLNSYKE
HHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEECCEEEEEECCCCCCCHHCCHHHHH
LEEERRLFYVAITRAEEHCYLSFAKSRFKYGKMEFSSPSRFLKDIDVHFLKLPQEEQMAR
HHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHEECCCHHHHHH
RIDERASRFCREQNERAARSLFDEPASQSSYGRSSSNISGGQKTFLVGERPKVEIIRPSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCC
PRNLTKVGPETVSKPSAARNLSVNVQAGQVIEHERFGIGDVIKVEGIGENSKATVRFRNA
CCHHHHCCCHHCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEECCCCCCEEEEECCC
GEKQLLLKFARFKVVE
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA