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The map label for this gene is pfkA1 [H]

Identifier: 150006014

GI number: 150006014

Start: 4369687

End: 4370670

Strand: Reverse

Name: pfkA1 [H]

Synonym: BVU_3512

Alternate gene names: 150006014

Gene position: 4370670-4369687 (Counterclockwise)

Preceding gene: 150006015

Following gene: 150006013

Centisome position: 84.65

GC content: 48.68

Gene sequence:

>984_bases
ATGGATAACAAGTATATTGGAATTCTGACTTCCGGAGGGGATGCTTCAGGAATGAACGCGGCTATCCGTGCCGTGACCCG
CGCCGCCATTTTTAACGGATTTAAAGTGAAAGGAATTTATAGAGGTTACGAGGGGCTGATTGCCGGTGAAGTGAAAGAAC
TCACGACGGAAGATGTAAGTAGCATTATCCAGAGGGGAGGCACCATACTGAAAACAGCTCGCTCCGAGACTTTCACCACT
CCCGAAGGACGCAAGAAAGCTTATAAGGTCATACAGAAAGAGAACATCAATGCTTTGATAATTATCGGTGGAGACGGTTC
CCTGACGGGAGCCCGTATCTTTGCGGAGGAGTATGATGTGACGTGCATAGGATTGCCCGGCACCATTGACAATGACCTAT
ATGGTACCGATTTTACCATAGGATATGATACGGCATTGAACACCATTGTGGAATGCGTGGATAAAATCAGGGACACGGCA
ACCTCCCATGACCGTATCTTCTTTGTCGAAGTAATGGGGCGTGATGCGGGCTTCCTGGCACAAAACAGCGCAATAGCTTC
CGGTGCTGAAGCGGCCATCATCCCGGAGGACAGGACGGACGTGGACCAGTTGGAAACATTCATCGGGCGCGGATTCAGAA
AGACGAAAAACAGCAGCATCGTAATTGTGACCGAAAGTCCTGAAAACAAGAATGGAGGTGCCATATATTACGCCGACCGG
GTAAAGAAGGAATATCCCGGGTATGATGTCAGGGTTTCTATCTTGGGCCATCTGCAACGGGGTGGTACTCCGAGTGCCAA
TGACCGTATACTGGCAAGCCGGCTGGGTGAAGCCGCAATCCAGGCCTTAATGGAAGACCAGCGGAATGTTATGATAGGCA
TACGGAACAACGAGATTGTTTATGTTCCTTTTGTCCAGGCAATCAAAAAGGACAAGACTATTGACAAAAGCCTGATCCGG
GTCCTTAACGAATTATCGATATAA

Upstream 100 bases:

>100_bases
TCACTGGGTACGATAAAAAGCCGTATCTTTTATAGCAGAAAAAGGCTAAAACAGTTGCTTTCTGATTTTGCCTGATTAAT
TAAAATATAAAAAACAAATT

Downstream 100 bases:

>100_bases
AATAGCCGTCAACATGTCAGAAGTTGCTCCCTTAATATCGGATCTTGCCATAATTCTGATTATTGCCGGTATTGTAACCG
TCATTTTCAAGTGGCTGAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGTPSANDRILASRLGEAAIQALMEDQRNVMIGIRNNEIVYVPFVQAIKKDKTIDKSLIR
VLNELSI

Sequences:

>Translated_327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGTPSANDRILASRLGEAAIQALMEDQRNVMIGIRNNEIVYVPFVQAIKKDKTIDKSLIR
VLNELSI
>Mature_327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGTPSANDRILASRLGEAAIQALMEDQRNVMIGIRNNEIVYVPFVQAIKKDKTIDKSLIR
VLNELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=349, Percent_Identity=43.8395415472779, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI266453619, Length=348, Percent_Identity=41.9540229885057, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI266453768, Length=348, Percent_Identity=41.9540229885057, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI266453748, Length=348, Percent_Identity=41.9540229885057, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI4505749, Length=348, Percent_Identity=41.9540229885057, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=41.9540229885057, Blast_Score=251, Evalue=8e-67,
Organism=Escherichia coli, GI1790350, Length=309, Percent_Identity=45.9546925566343, Blast_Score=245, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI25147584, Length=352, Percent_Identity=42.8977272727273, Blast_Score=243, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=38.3116883116883, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=339, Percent_Identity=30.3834808259587, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=40.1162790697674, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323861, Length=350, Percent_Identity=36.5714285714286, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28573326, Length=348, Percent_Identity=40.8045977011494, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=40.5172413793103, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35604; Mature: 35604

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
SIIQRGGTILKTARSETFTTPEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDV
HHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCE
TCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFLA
EEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEE
QNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHH
VKKEYPGYDVRVSILGHLQRGGTPSANDRILASRLGEAAIQALMEDQRNVMIGIRNNEIV
HHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
YVPFVQAIKKDKTIDKSLIRVLNELSI
EEHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
SIIQRGGTILKTARSETFTTPEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDV
HHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCE
TCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFLA
EEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEE
QNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHH
VKKEYPGYDVRVSILGHLQRGGTPSANDRILASRLGEAAIQALMEDQRNVMIGIRNNEIV
HHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
YVPFVQAIKKDKTIDKSLIRVLNELSI
EEHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]