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The map label for this gene is metF [H]

Identifier: 150005608

GI number: 150005608

Start: 3914750

End: 3915703

Strand: Direct

Name: metF [H]

Synonym: BVU_3096

Alternate gene names: 150005608

Gene position: 3914750-3915703 (Clockwise)

Preceding gene: 150005607

Following gene: 150005617

Centisome position: 75.82

GC content: 41.72

Gene sequence:

>954_bases
ATGGGTATTGCTGAGTTGATAAATAAGGAAAAGAAAACAGCATTTTCTTTCGAGGTTCTTCCACCGTTGAAAGGTACTGG
AATTGAAAAACTATATGCTACAATAGATACATTACGGGAGTTTGATCCTCTTTATGTCAATATTACCACTCATCGCAGCG
AATATGTGTATCGCGAGTTGGGGGGTGGCTTATATGAACGTAATCGTTATCGGCGGCGTCCGGGTACGGTAGCGGTGGCT
GCGGCTATACATAATAAATATGATATTACTGTTGTACCGCATATTTTGTGCAGTGGTTTCTCGCGTGAAGATACGGAATA
TATATTGCTTGATTTGCAATTCTTAGGTATTACTAATCTGTTGGTACTCCGGGGTGACAAGGCAAAAGATGAATCTTCTT
TTGTTCCCGAGAAAGATGGTTATGCCCATGCTTTGGAGTTGGAAGAGCAGATCAATGCTTTTAATGAAGGCAAATTTATT
GATGGAACGCCGATTCAGGCTCCTTTAACTCCTTTTCGTTATGGGGTGGCAGGCTATCCGGAGAAGCATGAGGAATCTCC
CAACATGGAACAAGATTTATATTGGTTAAAAAAGAAAGTGGAAGCCGGAGCCGATTATGTAGTGACACAAATGTTTTATG
ATAACCGAAAATACGTTGACTTTGTGGAGCGTGCAAGAAAAGAGGGGATTAATGTTCCTATCGTACCGGGCATCAAACCT
TTTTCCAAGCTATCACAACTCAGTGTTATTCCTAAAACATTCAATGTGGATTTACCACAGGAATTGGTTGTGGAAGCAAT
GAAATGCAAGGATGATAAAGAAGCGCGTGCATTAGGCATAGAGTGGTGTATCAATCAATGCCGGGAGCTGATAGCATATG
GGGTTCCGGGGATACATTTTTATACAGTGGGTGCGGTGGATAATGTGAAAGAGGTGGCGACAGCTGTTTATTGA

Upstream 100 bases:

>100_bases
AGAAATAAATCTGAAATGTGATTAATATAAAGATAAATCTTCTATATAGCTGTCTGTTATATGGATTTATCGGAATTAAT
AAGTAAAAAGGTCAAAAAAG

Downstream 100 bases:

>100_bases
TAGTCTTTAATGGAAATGTTTCTGTCAGGTCATTTATTGCTCCGTATCTTAACGGTTTAAGATGCGGAGCAAAATTATTT
TGTAAATGGGTTATAAGTCA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGIAELINKEKKTAFSFEVLPPLKGTGIEKLYATIDTLREFDPLYVNITTHRSEYVYRELGGGLYERNRYRRRPGTVAVA
AAIHNKYDITVVPHILCSGFSREDTEYILLDLQFLGITNLLVLRGDKAKDESSFVPEKDGYAHALELEEQINAFNEGKFI
DGTPIQAPLTPFRYGVAGYPEKHEESPNMEQDLYWLKKKVEAGADYVVTQMFYDNRKYVDFVERARKEGINVPIVPGIKP
FSKLSQLSVIPKTFNVDLPQELVVEAMKCKDDKEARALGIEWCINQCRELIAYGVPGIHFYTVGAVDNVKEVATAVY

Sequences:

>Translated_317_residues
MGIAELINKEKKTAFSFEVLPPLKGTGIEKLYATIDTLREFDPLYVNITTHRSEYVYRELGGGLYERNRYRRRPGTVAVA
AAIHNKYDITVVPHILCSGFSREDTEYILLDLQFLGITNLLVLRGDKAKDESSFVPEKDGYAHALELEEQINAFNEGKFI
DGTPIQAPLTPFRYGVAGYPEKHEESPNMEQDLYWLKKKVEAGADYVVTQMFYDNRKYVDFVERARKEGINVPIVPGIKP
FSKLSQLSVIPKTFNVDLPQELVVEAMKCKDDKEARALGIEWCINQCRELIAYGVPGIHFYTVGAVDNVKEVATAVY
>Mature_316_residues
GIAELINKEKKTAFSFEVLPPLKGTGIEKLYATIDTLREFDPLYVNITTHRSEYVYRELGGGLYERNRYRRRPGTVAVAA
AIHNKYDITVVPHILCSGFSREDTEYILLDLQFLGITNLLVLRGDKAKDESSFVPEKDGYAHALELEEQINAFNEGKFID
GTPIQAPLTPFRYGVAGYPEKHEESPNMEQDLYWLKKKVEAGADYVVTQMFYDNRKYVDFVERARKEGINVPIVPGIKPF
SKLSQLSVIPKTFNVDLPQELVVEAMKCKDDKEARALGIEWCINQCRELIAYGVPGIHFYTVGAVDNVKEVATAVY

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=304, Percent_Identity=31.5789473684211, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790377, Length=289, Percent_Identity=28.719723183391, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25147436, Length=317, Percent_Identity=27.7602523659306, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=317, Percent_Identity=27.7602523659306, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321313, Length=313, Percent_Identity=27.4760383386581, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325234, Length=336, Percent_Identity=26.7857142857143, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662658, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 35943; Mature: 35811

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAELINKEKKTAFSFEVLPPLKGTGIEKLYATIDTLREFDPLYVNITTHRSEYVYREL
CCHHHHHCHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
GGGLYERNRYRRRPGTVAVAAAIHNKYDITVVPHILCSGFSREDTEYILLDLQFLGITNL
CCCHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHCCCCCCCCEEEEEEEEHHCCCEE
LVLRGDKAKDESSFVPEKDGYAHALELEEQINAFNEGKFIDGTPIQAPLTPFRYGVAGYP
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
EKHEESPNMEQDLYWLKKKVEAGADYVVTQMFYDNRKYVDFVERARKEGINVPIVPGIKP
HHCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCH
FSKLSQLSVIPKTFNVDLPQELVVEAMKCKDDKEARALGIEWCINQCRELIAYGVPGIHF
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YTVGAVDNVKEVATAVY
EEECCHHHHHHHHHHCC
>Mature Secondary Structure 
GIAELINKEKKTAFSFEVLPPLKGTGIEKLYATIDTLREFDPLYVNITTHRSEYVYREL
CHHHHHCHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
GGGLYERNRYRRRPGTVAVAAAIHNKYDITVVPHILCSGFSREDTEYILLDLQFLGITNL
CCCHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHCCCCCCCCEEEEEEEEHHCCCEE
LVLRGDKAKDESSFVPEKDGYAHALELEEQINAFNEGKFIDGTPIQAPLTPFRYGVAGYP
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
EKHEESPNMEQDLYWLKKKVEAGADYVVTQMFYDNRKYVDFVERARKEGINVPIVPGIKP
HHCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCH
FSKLSQLSVIPKTFNVDLPQELVVEAMKCKDDKEARALGIEWCINQCRELIAYGVPGIHF
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YTVGAVDNVKEVATAVY
EEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]