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The map label for this gene is yfnG [H]

Identifier: 150005469

GI number: 150005469

Start: 3749672

End: 3750730

Strand: Direct

Name: yfnG [H]

Synonym: BVU_2952

Alternate gene names: 150005469

Gene position: 3749672-3750730 (Clockwise)

Preceding gene: 150005468

Following gene: 150005470

Centisome position: 72.62

GC content: 36.26

Gene sequence:

>1059_bases
ATGAATCTGATAGAAAATACTATGTATCAGGATGATATAATGAAAATTATAACTCATCCTCTTCCTTGGAATAAGTTGCA
GAATAAGGCTTTGTTAATTTCTGGAGCTACTGGACTTGTTGGCAGTTTCTTAATAGATGTTATCATGCTACTGAATAGGG
AGCAGGGGTTGAATTGTAAGGTTTATTCTCTTGGAAGGAATGAAGATAAGGCCAAATCAAGATTCAGTTATTGTTATGAA
TCTTCTTTATTTCAGTTTATATCATACGATATTAATCTGCCGTTTGTTAGAAATGATATTGGAAGTGTGGACTATGTTCT
CCATTTGGCTAGCAATACACATCCTGTTGCCTATGCTACAGATCCAATAGGAACCATAACAACCAATATTATTGGAACTC
AGAACATGTTGGAGTTTGCTTGTGCTCATCATGCATCACGCTGTGTTTTCGCTTCCTCAAATGAAATCTATGGTGAAAAT
CGAGGGGATATTGAGAAATTTGAAGAAAAATATTGTGGTTATATTGATTGTAACACGATTAGAGCAGGTTATCCTGAGAG
TAAAAGATGCGGTGAAGCTCTATGTCAGGCTTATATAAAACAAAGAGGGTTGGATATTGTGATTCCACGTCTAACTCGTT
CTTATGGTCCAACTATGTTGATGAGCGATACAAAAGCCATTTCCCAATTTATTTGTAAGGCTATTGTCGGAAAAGATATA
GTGCTTAAAAGTGAGGGAACTCAGTATTACTCTTATACATACGTGGCAGATGCTGTATGTGGGTTGTTGTATGTTTTGCT
GCTAGGGAAAAAAGGAGAGGCGTATAATATTGCTGACGAATCTTCAGATATTATGTTGAGAGATTTAGCGAAGATTATAG
CAGACTCTGTGGGTAATAAAGTCGTGTTTGAGATTCCTGATGCAATTGAAAGTATGGGATACAGCAAGGCAACAAAGGCA
AGACTAGACAGTACAAAACTTCAGGAACTTGGCTGGAAATCCATATATGATATTCAATCGGGGATACAAAGAACAATCAA
AATATTGAGTGAATTATGA

Upstream 100 bases:

>100_bases
TTGCCGCCGGTTCAGATAAAAATATTAAACTGACTACAAAAGATGATTTGGAAATGTTTAAGGGATATCTAAAGAAAGAA
AAGGATAATTGGTTGAAGTA

Downstream 100 bases:

>100_bases
CGAACGAATATGAGCATTTAAATGCAATACAGAAAATTGGGCTGAGTATATTGAAAGAGATTATTGCTATCTGTAAACGC
CATGATTTGACGTACTATGT

Product: putative epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNLIENTMYQDDIMKIITHPLPWNKLQNKALLISGATGLVGSFLIDVIMLLNREQGLNCKVYSLGRNEDKAKSRFSYCYE
SSLFQFISYDINLPFVRNDIGSVDYVLHLASNTHPVAYATDPIGTITTNIIGTQNMLEFACAHHASRCVFASSNEIYGEN
RGDIEKFEEKYCGYIDCNTIRAGYPESKRCGEALCQAYIKQRGLDIVIPRLTRSYGPTMLMSDTKAISQFICKAIVGKDI
VLKSEGTQYYSYTYVADAVCGLLYVLLLGKKGEAYNIADESSDIMLRDLAKIIADSVGNKVVFEIPDAIESMGYSKATKA
RLDSTKLQELGWKSIYDIQSGIQRTIKILSEL

Sequences:

>Translated_352_residues
MNLIENTMYQDDIMKIITHPLPWNKLQNKALLISGATGLVGSFLIDVIMLLNREQGLNCKVYSLGRNEDKAKSRFSYCYE
SSLFQFISYDINLPFVRNDIGSVDYVLHLASNTHPVAYATDPIGTITTNIIGTQNMLEFACAHHASRCVFASSNEIYGEN
RGDIEKFEEKYCGYIDCNTIRAGYPESKRCGEALCQAYIKQRGLDIVIPRLTRSYGPTMLMSDTKAISQFICKAIVGKDI
VLKSEGTQYYSYTYVADAVCGLLYVLLLGKKGEAYNIADESSDIMLRDLAKIIADSVGNKVVFEIPDAIESMGYSKATKA
RLDSTKLQELGWKSIYDIQSGIQRTIKILSEL
>Mature_352_residues
MNLIENTMYQDDIMKIITHPLPWNKLQNKALLISGATGLVGSFLIDVIMLLNREQGLNCKVYSLGRNEDKAKSRFSYCYE
SSLFQFISYDINLPFVRNDIGSVDYVLHLASNTHPVAYATDPIGTITTNIIGTQNMLEFACAHHASRCVFASSNEIYGEN
RGDIEKFEEKYCGYIDCNTIRAGYPESKRCGEALCQAYIKQRGLDIVIPRLTRSYGPTMLMSDTKAISQFICKAIVGKDI
VLKSEGTQYYSYTYVADAVCGLLYVLLLGKKGEAYNIADESSDIMLRDLAKIIADSVGNKVVFEIPDAIESMGYSKATKA
RLDSTKLQELGWKSIYDIQSGIQRTIKILSEL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=30.3834808259587, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=24.9266862170088, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=24.8554913294798, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=25.4335260115607, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=243, Percent_Identity=34.9794238683128, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=26.8882175226586, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=24.773413897281, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=30.625, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39370; Mature: 39370

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIENTMYQDDIMKIITHPLPWNKLQNKALLISGATGLVGSFLIDVIMLLNREQGLNCK
CCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEE
VYSLGRNEDKAKSRFSYCYESSLFQFISYDINLPFVRNDIGSVDYVLHLASNTHPVAYAT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCCCEEEEC
DPIGTITTNIIGTQNMLEFACAHHASRCVFASSNEIYGENRGDIEKFEEKYCGYIDCNTI
CCCHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCEEECCCE
RAGYPESKRCGEALCQAYIKQRGLDIVIPRLTRSYGPTMLMSDTKAISQFICKAIVGKDI
ECCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCE
VLKSEGTQYYSYTYVADAVCGLLYVLLLGKKGEAYNIADESSDIMLRDLAKIIADSVGNK
EEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCE
VVFEIPDAIESMGYSKATKARLDSTKLQELGWKSIYDIQSGIQRTIKILSEL
EEEECCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLIENTMYQDDIMKIITHPLPWNKLQNKALLISGATGLVGSFLIDVIMLLNREQGLNCK
CCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEE
VYSLGRNEDKAKSRFSYCYESSLFQFISYDINLPFVRNDIGSVDYVLHLASNTHPVAYAT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCCCEEEEC
DPIGTITTNIIGTQNMLEFACAHHASRCVFASSNEIYGENRGDIEKFEEKYCGYIDCNTI
CCCHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHCCEEECCCE
RAGYPESKRCGEALCQAYIKQRGLDIVIPRLTRSYGPTMLMSDTKAISQFICKAIVGKDI
ECCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCE
VLKSEGTQYYSYTYVADAVCGLLYVLLLGKKGEAYNIADESSDIMLRDLAKIIADSVGNK
EEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCE
VVFEIPDAIESMGYSKATKARLDSTKLQELGWKSIYDIQSGIQRTIKILSEL
EEEECCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]