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The map label for this gene is rpoD [H]

Identifier: 150005266

GI number: 150005266

Start: 3494918

End: 3495778

Strand: Direct

Name: rpoD [H]

Synonym: BVU_2738

Alternate gene names: 150005266

Gene position: 3494918-3495778 (Clockwise)

Preceding gene: 150005265

Following gene: 150005267

Centisome position: 67.69

GC content: 45.76

Gene sequence:

>861_bases
ATGAGACAATTAAAGATTACCAAAAGTATCACTAACCGAGAGAGCGCATCTCTGGATAAGTATTTACAGGAAATAGGCCG
TGAAGACCTGATTACTGTAGAGGAAGAGGTAGAGCTCGCTCAGCGTATCCGTAAAGGCGACCGGGTAGCATTGGAAAAAC
TGACACGTGCCAACTTGCGTTTCGTCGTGTCTGTAGCCAAGCAGTATCAAAATCAGGGGTTAAGTCTTCCTGACTTGATT
AATGAAGGCAACCTGGGATTGATTAAAGCAGCCGAAAAGTTCGATGAAACCCGAGGCTTCAAGTTTATCAGTTACGCCGT
ATGGTGGATTCGTCAGTCTATTCTCCAGGCATTGGCAGAACAGTCACGTATTGTCCGTTTGCCGTTGAATCAGGTAGGCT
CGTTGAACAAGATCAGCAAAGCCTTCTCTAAATTCGAACAGGAAAACGAACGTCGTCCTTCTCCGGAAGAACTGGCCGAT
GAACTGGAAATACCTGTAGACAAAATCTCGGACACGTTGAAGGTTTCAGGACGCCACATTTCTGTAGACGCTCCTTTTGT
GGAAGGAGAAGACAACAGCTTGCTGGATGTGCTTGTGAACGACGACTCGCCTATGGCAGACCGTTCCTTGGTTAATGAGT
CACTTGCGAGGGAAATTGATAGAGCACTCTCTACGCTGACCGAAAGGGAGAAGGATATTATCCAGATGTTTTTCGGTATT
AATACGCAGGAAATGACGTTGGAAGAAATCGGCGATAAATTCGGCCTCACTCGTGAGCGCGTACGCCAGATTAAAGAGAA
AGCCATTAGAAGATTGAGACAAAATTCGCGTAGCAAGCTGCTTAAGTCTTATTTAGGGTAA

Upstream 100 bases:

>100_bases
AGATAGTCTTTTTTGATTTATTAGCTAATATTTTTCAAACAAAAATGGATGTATTCCATTTAATGTTGTAAATTTGCATC
CCAAATTTGTTTTATAGAGA

Downstream 100 bases:

>100_bases
GAAAGATTCGGTTTCCGAAAAGAAAACAAAGCACAAGATGGAAGGTTGCTTCCGTCTTGTGCTTTTGTTCATAATATACC
AATAATGCCTATCGGCTGCA

Product: RNA polymerase sigma factor rpoD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIRKGDRVALEKLTRANLRFVVSVAKQYQNQGLSLPDLI
NEGNLGLIKAAEKFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKAFSKFEQENERRPSPEELAD
ELEIPVDKISDTLKVSGRHISVDAPFVEGEDNSLLDVLVNDDSPMADRSLVNESLAREIDRALSTLTEREKDIIQMFFGI
NTQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRQNSRSKLLKSYLG

Sequences:

>Translated_286_residues
MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIRKGDRVALEKLTRANLRFVVSVAKQYQNQGLSLPDLI
NEGNLGLIKAAEKFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKAFSKFEQENERRPSPEELAD
ELEIPVDKISDTLKVSGRHISVDAPFVEGEDNSLLDVLVNDDSPMADRSLVNESLAREIDRALSTLTEREKDIIQMFFGI
NTQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRQNSRSKLLKSYLG
>Mature_286_residues
MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIRKGDRVALEKLTRANLRFVVSVAKQYQNQGLSLPDLI
NEGNLGLIKAAEKFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKAFSKFEQENERRPSPEELAD
ELEIPVDKISDTLKVSGRHISVDAPFVEGEDNSLLDVLVNDDSPMADRSLVNESLAREIDRALSTLTEREKDIIQMFFGI
NTQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRQNSRSKLLKSYLG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This may be the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=268, Percent_Identity=41.7910447761194, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=39.6296296296296, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=27.2401433691756, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=29.064039408867, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32804; Mature: 32804

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIRKGDRVALEKLTRANLR
CCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKFISYAVWWIRQSILQALAE
HHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QSRIVRLPLNQVGSLNKISKAFSKFEQENERRPSPEELADELEIPVDKISDTLKVSGRHI
CCCEEECCHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEE
SVDAPFVEGEDNSLLDVLVNDDSPMADRSLVNESLAREIDRALSTLTEREKDIIQMFFGI
EECCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NTQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRQNSRSKLLKSYLG
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIRKGDRVALEKLTRANLR
CCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKFISYAVWWIRQSILQALAE
HHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QSRIVRLPLNQVGSLNKISKAFSKFEQENERRPSPEELADELEIPVDKISDTLKVSGRHI
CCCEEECCHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEE
SVDAPFVEGEDNSLLDVLVNDDSPMADRSLVNESLAREIDRALSTLTEREKDIIQMFFGI
EECCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NTQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRQNSRSKLLKSYLG
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1923759 [H]