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The map label for this gene is gacS [H]

Identifier: 150005253

GI number: 150005253

Start: 3469425

End: 3471428

Strand: Direct

Name: gacS [H]

Synonym: BVU_2725

Alternate gene names: 150005253

Gene position: 3469425-3471428 (Clockwise)

Preceding gene: 150005249

Following gene: 150005254

Centisome position: 67.2

GC content: 43.96

Gene sequence:

>2004_bases
ATGCCAATTGGTTGTGTTCTGTTTTTATTTTATTATCTTTGCAGCGAATTTTTAGAAATTATTCTAAAGGAAATGAGGGG
ATTGGCAATCATATTCAGGTTTTTTATGCTATTAGTCCTGCTGCTTCCTGTGGTTGCTCTGTGTCCTGTGAAGGCGGAAA
CGACCTCGGAAGGGCCTATCCTTATTGTCACTTCTTATAATCCCGAAACGCGTAGTATTTCTGACAACCTGTCTGCTTTT
ATGGATGAATACAGACAGCGTGGCGGCAAGTATACTCCGATCATAGAGAGTATGAACTGCAAGAATTTGTCCGAGGCCTA
TCTGTGGAAATCGCGTATGGCCTCCATCCTTGGTAAGTATAAGGGGAAAAACCGTCCGTCACTGGTTATATTGTTGGGGC
AGGAGGCATGGTCGGCTTATATCTCACAAGACACAGAGATAGCTAAAAAGACTCCGTCTATCTGTGGCATGGTCAGTGTC
AACGGACTGGTATTGCCCGATGATAGTATCGACACGCGGGTATGGGAACCTGAAAGTAAGAATATTTATACGGATTTCAG
TGATTATAATATTGTTGCCGGTTATGTATATGAATATGATGTCGATAAAAACATAAAGCTGATGCGTCGTTTTTATCCTG
ATATGCGCCGTGTGGCATTTATATCGGATAATACCTATGGCGGGTTGTCTATGCAAGCCTTGGTTAAAAAGGAGATGAAG
AAGTACCCCGATTTAGAAACAATTTGGCTGGACGGCCGTACAGAAACCTTTATGGAAGTGAGTGAAAGGATGCGCAGGTT
GCCTCAAAATACGTGTGTGTTATTGGGAACATGGCGTGTAGACTGTACGGAGAGTTATGTTATTGGTAATACGACTTATA
TGCTGCGCGATGCGAATCCCACCCTGCCTGTGTTTACTATTGCATCTGTGGGGCTGGGACATTGGGCATTGGGAGGATAT
ACTCCCGAGTACCATGCGGTAGGAAAGAATATAGGCGCGGTTACTTATGATTTTTTGGATAAAGGGGATAGAGAAGGGGT
GGACTTGTTGACTATTCCCGGAAACTATACTTTTGATATAAAGCGTTTGCATGAATTCAAATTGGATTCATTGAATCTTC
CTCAGGGAGCGGTGTTGGTGAACAAGACTCCTTCGTTTTATGAGCAGTATAAATATTGGGTCATAGGGGTTGTTTCGGCG
TTCATGTTCCTGATAGCCTGTTTCTTGATAGCCATATATTATATTATCCGTATCAACCACTTGAAACATAATCTGGAAGT
ATCCGGCGAGGAACTGCTGGTAGCCAAGGAGAAAGCGGAGGAATCCAACAGGTTGAAGACGGCTTTTCTGGCCAATATGA
GTCACGAAATACGCACTCCCTTGAATGCCATTGTGGGATTTTCCAGCGTGTTGGTCAGTGATGATTCCTCTCCTGCCGAG
AAAGAGCAGTATTGTGATATCATTCAGAAGAATTCCGATTTGTTGTTGCACCTCATAAATGATATTCTGGACATCTCGCG
CATGGAATCCGGAAGGATAAAATTCGTATGGGAAGAATGTGATGTGGTAGAGTTGTGTCAGACGGCTTTGTCTACAGTGG
AGTATGGACGCAAGACCAGTGCTTTGTTTCTGTTTGAAACTCCTGTGCCTTCGCTGGTTGTCAAGACGGATGCCCAGCGT
CTGAAGCAGGTGTTGATTAATTTGCTGTCCAATGCCGCAAAATTTACTCCGTCCGGTTCTATCAAACTGACGGTTGCCAT
AGACAAACAGCATCAGCAGTTGGAATTGTCAGTGTCGGATACCGGTTGCGGTATTCCGCCCGATAAGAGTGAAAAGGTAT
TTGAACGTTTCGAGAAATTGAATGAATATTCCCAAGGTACGGGGCTGGGATTGGCCATTAGCAGGCTGATAATCGAGAAT
CTGGGAGGTAAGATATGGGTGGACAAGGATTATACCGAAGGCGCCCGGTTTGTATTCACGCATCCTTTGACTAAAAAAGA
ATAA

Upstream 100 bases:

>100_bases
TTGGGTGGCGCTTTTGTCCACTCCCAATACTTTGTAATAGTCTATGTAAGCCATATTATATATTTGTTAAATTTCATCTG
CTTTAAGAGAAACAAAATAC

Downstream 100 bases:

>100_bases
AGAATAGAGAAATGAAATATAGAATATGTATCAGTGCCCTGCTGTTGTGGGCAGGTATGCAGTTGTCTGCATCCAATAAT
CAGGAAGAAGTGGTTATCAA

Product: putative two-component histidine sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MPIGCVLFLFYYLCSEFLEIILKEMRGLAIIFRFFMLLVLLLPVVALCPVKAETTSEGPILIVTSYNPETRSISDNLSAF
MDEYRQRGGKYTPIIESMNCKNLSEAYLWKSRMASILGKYKGKNRPSLVILLGQEAWSAYISQDTEIAKKTPSICGMVSV
NGLVLPDDSIDTRVWEPESKNIYTDFSDYNIVAGYVYEYDVDKNIKLMRRFYPDMRRVAFISDNTYGGLSMQALVKKEMK
KYPDLETIWLDGRTETFMEVSERMRRLPQNTCVLLGTWRVDCTESYVIGNTTYMLRDANPTLPVFTIASVGLGHWALGGY
TPEYHAVGKNIGAVTYDFLDKGDREGVDLLTIPGNYTFDIKRLHEFKLDSLNLPQGAVLVNKTPSFYEQYKYWVIGVVSA
FMFLIACFLIAIYYIIRINHLKHNLEVSGEELLVAKEKAEESNRLKTAFLANMSHEIRTPLNAIVGFSSVLVSDDSSPAE
KEQYCDIIQKNSDLLLHLINDILDISRMESGRIKFVWEECDVVELCQTALSTVEYGRKTSALFLFETPVPSLVVKTDAQR
LKQVLINLLSNAAKFTPSGSIKLTVAIDKQHQQLELSVSDTGCGIPPDKSEKVFERFEKLNEYSQGTGLGLAISRLIIEN
LGGKIWVDKDYTEGARFVFTHPLTKKE

Sequences:

>Translated_667_residues
MPIGCVLFLFYYLCSEFLEIILKEMRGLAIIFRFFMLLVLLLPVVALCPVKAETTSEGPILIVTSYNPETRSISDNLSAF
MDEYRQRGGKYTPIIESMNCKNLSEAYLWKSRMASILGKYKGKNRPSLVILLGQEAWSAYISQDTEIAKKTPSICGMVSV
NGLVLPDDSIDTRVWEPESKNIYTDFSDYNIVAGYVYEYDVDKNIKLMRRFYPDMRRVAFISDNTYGGLSMQALVKKEMK
KYPDLETIWLDGRTETFMEVSERMRRLPQNTCVLLGTWRVDCTESYVIGNTTYMLRDANPTLPVFTIASVGLGHWALGGY
TPEYHAVGKNIGAVTYDFLDKGDREGVDLLTIPGNYTFDIKRLHEFKLDSLNLPQGAVLVNKTPSFYEQYKYWVIGVVSA
FMFLIACFLIAIYYIIRINHLKHNLEVSGEELLVAKEKAEESNRLKTAFLANMSHEIRTPLNAIVGFSSVLVSDDSSPAE
KEQYCDIIQKNSDLLLHLINDILDISRMESGRIKFVWEECDVVELCQTALSTVEYGRKTSALFLFETPVPSLVVKTDAQR
LKQVLINLLSNAAKFTPSGSIKLTVAIDKQHQQLELSVSDTGCGIPPDKSEKVFERFEKLNEYSQGTGLGLAISRLIIEN
LGGKIWVDKDYTEGARFVFTHPLTKKE
>Mature_666_residues
PIGCVLFLFYYLCSEFLEIILKEMRGLAIIFRFFMLLVLLLPVVALCPVKAETTSEGPILIVTSYNPETRSISDNLSAFM
DEYRQRGGKYTPIIESMNCKNLSEAYLWKSRMASILGKYKGKNRPSLVILLGQEAWSAYISQDTEIAKKTPSICGMVSVN
GLVLPDDSIDTRVWEPESKNIYTDFSDYNIVAGYVYEYDVDKNIKLMRRFYPDMRRVAFISDNTYGGLSMQALVKKEMKK
YPDLETIWLDGRTETFMEVSERMRRLPQNTCVLLGTWRVDCTESYVIGNTTYMLRDANPTLPVFTIASVGLGHWALGGYT
PEYHAVGKNIGAVTYDFLDKGDREGVDLLTIPGNYTFDIKRLHEFKLDSLNLPQGAVLVNKTPSFYEQYKYWVIGVVSAF
MFLIACFLIAIYYIIRINHLKHNLEVSGEELLVAKEKAEESNRLKTAFLANMSHEIRTPLNAIVGFSSVLVSDDSSPAEK
EQYCDIIQKNSDLLLHLINDILDISRMESGRIKFVWEECDVVELCQTALSTVEYGRKTSALFLFETPVPSLVVKTDAQRL
KQVLINLLSNAAKFTPSGSIKLTVAIDKQHQQLELSVSDTGCGIPPDKSEKVFERFEKLNEYSQGTGLGLAISRLIIENL
GGKIWVDKDYTEGARFVFTHPLTKKE

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=32.7935222672065, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI48994928, Length=267, Percent_Identity=34.8314606741573, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=33.8912133891213, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=35.7692307692308, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=34.7457627118644, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=32.3144104803493, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=25.8333333333333, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788393, Length=210, Percent_Identity=28.0952380952381, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=26.5765765765766, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1790861, Length=208, Percent_Identity=31.25, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=26.271186440678, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787894, Length=244, Percent_Identity=28.6885245901639, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75567; Mature: 75436

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIGCVLFLFYYLCSEFLEIILKEMRGLAIIFRFFMLLVLLLPVVALCPVKAETTSEGPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LIVTSYNPETRSISDNLSAFMDEYRQRGGKYTPIIESMNCKNLSEAYLWKSRMASILGKY
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
KGKNRPSLVILLGQEAWSAYISQDTEIAKKTPSICGMVSVNGLVLPDDSIDTRVWEPESK
CCCCCCEEEEEECCHHHHHHHCCCHHHHHCCCCCEEEEEECCEEECCCCCCCCEECCCCC
NIYTDFSDYNIVAGYVYEYDVDKNIKLMRRFYPDMRRVAFISDNTYGGLSMQALVKKEMK
CEEECCCCCCEEEEEEEEECCCCCHHHHHHHCCCHHEEEEEECCCCCCCHHHHHHHHHHH
KYPDLETIWLDGRTETFMEVSERMRRLPQNTCVLLGTWRVDCTESYVIGNTTYMLRDANP
HCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEEECCCC
TLPVFTIASVGLGHWALGGYTPEYHAVGKNIGAVTYDFLDKGDREGVDLLTIPGNYTFDI
CCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEHHHCCCCCCCCEEEECCCCCEEEH
KRLHEFKLDSLNLPQGAVLVNKTPSFYEQYKYWVIGVVSAFMFLIACFLIAIYYIIRINH
HHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHNLEVSGEELLVAKEKAEESNRLKTAFLANMSHEIRTPLNAIVGFSSVLVSDDSSPAE
HHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCHHHEEEECCCCCCH
KEQYCDIIQKNSDLLLHLINDILDISRMESGRIKFVWEECDVVELCQTALSTVEYGRKTS
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC
ALFLFETPVPSLVVKTDAQRLKQVLINLLSNAAKFTPSGSIKLTVAIDKQHQQLELSVSD
EEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECC
TGCGIPPDKSEKVFERFEKLNEYSQGTGLGLAISRLIIENLGGKIWVDKDYTEGARFVFT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
HPLTKKE
CCCCCCC
>Mature Secondary Structure 
PIGCVLFLFYYLCSEFLEIILKEMRGLAIIFRFFMLLVLLLPVVALCPVKAETTSEGPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LIVTSYNPETRSISDNLSAFMDEYRQRGGKYTPIIESMNCKNLSEAYLWKSRMASILGKY
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
KGKNRPSLVILLGQEAWSAYISQDTEIAKKTPSICGMVSVNGLVLPDDSIDTRVWEPESK
CCCCCCEEEEEECCHHHHHHHCCCHHHHHCCCCCEEEEEECCEEECCCCCCCCEECCCCC
NIYTDFSDYNIVAGYVYEYDVDKNIKLMRRFYPDMRRVAFISDNTYGGLSMQALVKKEMK
CEEECCCCCCEEEEEEEEECCCCCHHHHHHHCCCHHEEEEEECCCCCCCHHHHHHHHHHH
KYPDLETIWLDGRTETFMEVSERMRRLPQNTCVLLGTWRVDCTESYVIGNTTYMLRDANP
HCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCEEEEEECCCC
TLPVFTIASVGLGHWALGGYTPEYHAVGKNIGAVTYDFLDKGDREGVDLLTIPGNYTFDI
CCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEHHHCCCCCCCCEEEECCCCCEEEH
KRLHEFKLDSLNLPQGAVLVNKTPSFYEQYKYWVIGVVSAFMFLIACFLIAIYYIIRINH
HHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHNLEVSGEELLVAKEKAEESNRLKTAFLANMSHEIRTPLNAIVGFSSVLVSDDSSPAE
HHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCHHHEEEECCCCCCH
KEQYCDIIQKNSDLLLHLINDILDISRMESGRIKFVWEECDVVELCQTALSTVEYGRKTS
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC
ALFLFETPVPSLVVKTDAQRLKQVLINLLSNAAKFTPSGSIKLTVAIDKQHQQLELSVSD
EEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECC
TGCGIPPDKSEKVFERFEKLNEYSQGTGLGLAISRLIIENLGGKIWVDKDYTEGARFVFT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
HPLTKKE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]